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The map label for this gene is hldE [H]

Identifier: 150400833

GI number: 150400833

Start: 415964

End: 416440

Strand: Reverse

Name: hldE [H]

Synonym: Maeo_0401

Alternate gene names: 150400833

Gene position: 416440-415964 (Counterclockwise)

Preceding gene: 150400834

Following gene: 150400832

Centisome position: 26.53

GC content: 25.58

Gene sequence:

>477_bases
ATGATAATACAAAATAAAAATTTACTTAATAATATGGTAAATGAATTAAAAAATCAAAATTTAAAAATAGTTTTTACAAA
TGGATGTTTTGATATACTACATAAAGGACATGTGGAATATTTAAATCATGCTAAAAAATTTGGAGATATATTAATAGTTG
GAATTAATTCAGATAAATCAATAAAAAATATAAAAGGAGATAAAAGACCAATTATACCATTGGAGTCAAGAATGTATGTT
TTGGACAGTTTAAAATCGGTTGATTTTATAGTTCCATTTGAAGAGGATACTCCAATGGACCTAATTGAAATTATAAAACC
AGCTGTGCATGTAAAGGGTGGAGATTATTCTGTGGAAGATTTACCCGAATCAAAAATAATTAAAAAATACGGCGGAAAAA
TAAAGATAATCCCATTTATTGAAGGATTTTCCACAACTACCGTAATTGAATGGGTTTTGGAAAAATATAAATCTTAA

Upstream 100 bases:

>100_bases
CACTAATAGTTAATTCAAATAATACCCCAAGAATACAGGAATGTCATTTAACAATATATCATGTTATATGTGAAGAAGTA
GAAAAAGAATTGGTAAAATT

Downstream 100 bases:

>100_bases
TAATTTTAATTGATTAAGAAACAACTTTTAAAAACTATAAAAGGAGGTAAAAAATGATTTGTGTTTTGGGGGATATAATG
CTTGATAAATATACCTATGG

Product: rfaE bifunctional protein

Products: diphosphate; CDPglycerol

Alternate protein names: D-beta-D-heptose 7-phosphate kinase; D-beta-D-heptose 7-phosphotransferase; D-beta-D-heptose 1-phosphate adenosyltransferase [H]

Number of amino acids: Translated: 158; Mature: 158

Protein sequence:

>158_residues
MIIQNKNLLNNMVNELKNQNLKIVFTNGCFDILHKGHVEYLNHAKKFGDILIVGINSDKSIKNIKGDKRPIIPLESRMYV
LDSLKSVDFIVPFEEDTPMDLIEIIKPAVHVKGGDYSVEDLPESKIIKKYGGKIKIIPFIEGFSTTTVIEWVLEKYKS

Sequences:

>Translated_158_residues
MIIQNKNLLNNMVNELKNQNLKIVFTNGCFDILHKGHVEYLNHAKKFGDILIVGINSDKSIKNIKGDKRPIIPLESRMYV
LDSLKSVDFIVPFEEDTPMDLIEIIKPAVHVKGGDYSVEDLPESKIIKKYGGKIKIIPFIEGFSTTTVIEWVLEKYKS
>Mature_158_residues
MIIQNKNLLNNMVNELKNQNLKIVFTNGCFDILHKGHVEYLNHAKKFGDILIVGINSDKSIKNIKGDKRPIIPLESRMYV
LDSLKSVDFIVPFEEDTPMDLIEIIKPAVHVKGGDYSVEDLPESKIIKKYGGKIKIIPFIEGFSTTTVIEWVLEKYKS

Specific function: Catalyzes the ADP transfer to D-glycero-D-manno-heptose 1-phosphate, yielding ADP-D,D-heptose [H]

COG id: COG2870

COG function: function code M; ADP-heptose synthase, bifunctional sugar kinase/adenylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the cytidylyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789432, Length=136, Percent_Identity=52.2058823529412, Blast_Score=146, Evalue=7e-37,
Organism=Caenorhabditis elegans, GI17509873, Length=76, Percent_Identity=40.7894736842105, Blast_Score=65, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI193203447, Length=76, Percent_Identity=40.7894736842105, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023030
- InterPro:   IPR011611
- InterPro:   IPR002173
- InterPro:   IPR004821
- InterPro:   IPR004820
- InterPro:   IPR011913
- InterPro:   IPR011914
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01467 CTP_transf_2; PF00294 PfkB [H]

EC number: 2.7.7.39

Molecular weight: Translated: 18017; Mature: 18017

Theoretical pI: Translated: 8.43; Mature: 8.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIQNKNLLNNMVNELKNQNLKIVFTNGCFDILHKGHVEYLNHAKKFGDILIVGINSDKS
CEECCHHHHHHHHHHHCCCCEEEEEECCHHHHHHCCHHHHHHHHHHCCCEEEEEECCCCC
IKNIKGDKRPIIPLESRMYVLDSLKSVDFIVPFEEDTPMDLIEIIKPAVHVKGGDYSVED
HHHCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHEEECCCCCCHHH
LPESKIIKKYGGKIKIIPFIEGFSTTTVIEWVLEKYKS
CCHHHHHHHHCCEEEEEEEECCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MIIQNKNLLNNMVNELKNQNLKIVFTNGCFDILHKGHVEYLNHAKKFGDILIVGINSDKS
CEECCHHHHHHHHHHHCCCCEEEEEECCHHHHHHCCHHHHHHHHHHCCCEEEEEECCCCC
IKNIKGDKRPIIPLESRMYVLDSLKSVDFIVPFEEDTPMDLIEIIKPAVHVKGGDYSVED
HHHCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHEEECCCCCCHHH
LPESKIIKKYGGKIKIIPFIEGFSTTTVIEWVLEKYKS
CCHHHHHHHHCCEEEEEEEECCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: CTP; sn-glycerol 3-phosphate

Specific reaction: CTP + sn-glycerol 3-phosphate = diphosphate + CDP-glycerol

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA