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The map label for this gene is rfbJ [H]

Identifier: 150400818

GI number: 150400818

Start: 399167

End: 400063

Strand: Direct

Name: rfbJ [H]

Synonym: Maeo_0386

Alternate gene names: 150400818

Gene position: 399167-400063 (Clockwise)

Preceding gene: 150400817

Following gene: 150400819

Centisome position: 25.43

GC content: 27.09

Gene sequence:

>897_bases
TTGTCTAAAACAATATTATTAACAGGGGCCACTGGGTTCTTGGGAAGTCATTTATTGGAAATGTTGGTAAAAGAAGGGAA
TAATGTTATCATTTTAAAGCGTAGTTTTTCCAATATATCAAGAATTAGACATCTCTTAGGGGATGTTAAGTATTACGATA
TTGATAAAGTTGAATTAAAAACACCTTTTGCAGAAAATGAAATAGATATGGTAATACATACTGCCACATCCTATGGTAGA
AAAAACGAAAAAATTAGTGAAATTGTAGAAACTAATCTAATGCTTCCATTAAAACTATTAGAATTATGCACATTTTTTAA
TACTGACACATTTTTTAATACTGACACAATTTTACCTAAAAACTTAAATTACTATGTGTTATCAAAAAAGCAATTTTTAG
AATATGGGAAAAGAATAAGTGATGAATGTAAATTAAGATTTATAAATATGAAGTTAGAACACATGTATGGTCCAAAAGAC
GATAATACAAAATTTATCCCATATATAATTGAAAAGATGTTAAAAAATGAGAAAGAAATCAACCTAACAAAAGGGGAACA
GAAAAGGGATTTTATATATGTTGAGGATGTAGCTAACGCATATGCAACTATTCTGTCCAAAATAAATAGTTTTGATAAAA
AGTTTTATGATATTGAAGTAGGGACTGGTAACCCAGTTAAAATAAAAGATTTAGTTATGCTGATAAAAAATTTATGTAAT
TCAAATATAAAATTGAATTTTGGAGCAATTCCATATAGAAAAAATGAAATTATGAACTCTGACGCAAATCCTGAATTTTT
AAGGAATTTGGGATGGTTTCCTAAGTTTTCATTGGATGAAGGTTTAAGAAAAACAATATCTTACTATCAAAAACATCTGG
TGAAGAATGATGAATAA

Upstream 100 bases:

>100_bases
ATGGTTTCTGGAAACCTATGGATAAATTAAAAGATAAGATGGAATTGGAAGAAATGTGGAATTCAGGTAATGCACCATGG
AAAATATGGGGTGAGTAATA

Downstream 100 bases:

>100_bases
TAATGCAAATGATTCTTTGTGCAAATTTTTCAAAGGAAAAAAAATTCTCATTACAGGACATACAGGGTTTAAAGGTAGTT
GGCTAACACAGGTTTTATTG

Product: NAD-dependent epimerase/dehydratase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MSKTILLTGATGFLGSHLLEMLVKEGNNVIILKRSFSNISRIRHLLGDVKYYDIDKVELKTPFAENEIDMVIHTATSYGR
KNEKISEIVETNLMLPLKLLELCTFFNTDTFFNTDTILPKNLNYYVLSKKQFLEYGKRISDECKLRFINMKLEHMYGPKD
DNTKFIPYIIEKMLKNEKEINLTKGEQKRDFIYVEDVANAYATILSKINSFDKKFYDIEVGTGNPVKIKDLVMLIKNLCN
SNIKLNFGAIPYRKNEIMNSDANPEFLRNLGWFPKFSLDEGLRKTISYYQKHLVKNDE

Sequences:

>Translated_298_residues
MSKTILLTGATGFLGSHLLEMLVKEGNNVIILKRSFSNISRIRHLLGDVKYYDIDKVELKTPFAENEIDMVIHTATSYGR
KNEKISEIVETNLMLPLKLLELCTFFNTDTFFNTDTILPKNLNYYVLSKKQFLEYGKRISDECKLRFINMKLEHMYGPKD
DNTKFIPYIIEKMLKNEKEINLTKGEQKRDFIYVEDVANAYATILSKINSFDKKFYDIEVGTGNPVKIKDLVMLIKNLCN
SNIKLNFGAIPYRKNEIMNSDANPEFLRNLGWFPKFSLDEGLRKTISYYQKHLVKNDE
>Mature_297_residues
SKTILLTGATGFLGSHLLEMLVKEGNNVIILKRSFSNISRIRHLLGDVKYYDIDKVELKTPFAENEIDMVIHTATSYGRK
NEKISEIVETNLMLPLKLLELCTFFNTDTFFNTDTILPKNLNYYVLSKKQFLEYGKRISDECKLRFINMKLEHMYGPKDD
NTKFIPYIIEKMLKNEKEINLTKGEQKRDFIYVEDVANAYATILSKINSFDKKFYDIEVGTGNPVKIKDLVMLIKNLCNS
NIKLNFGAIPYRKNEIMNSDANPEFLRNLGWFPKFSLDEGLRKTISYYQKHLVKNDE

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI42516563, Length=318, Percent_Identity=24.5283018867925, Blast_Score=82, Evalue=8e-16,
Organism=Homo sapiens, GI7657641, Length=337, Percent_Identity=26.1127596439169, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71982035, Length=329, Percent_Identity=24.0121580547112, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71982038, Length=331, Percent_Identity=23.8670694864048, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI21356223, Length=327, Percent_Identity=25.3822629969419, Blast_Score=89, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 34693; Mature: 34562

Theoretical pI: Translated: 9.08; Mature: 9.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKTILLTGATGFLGSHLLEMLVKEGNNVIILKRSFSNISRIRHLLGDVKYYDIDKVELK
CCCEEEEECCHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHCCCEEECCCEEEEE
TPFAENEIDMVIHTATSYGRKNEKISEIVETNLMLPLKLLELCTFFNTDTFFNTDTILPK
CCCCCCCCEEEEEECHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEECCCCCCCC
NLNYYVLSKKQFLEYGKRISDECKLRFINMKLEHMYGPKDDNTKFIPYIIEKMLKNEKEI
CCCEEEECHHHHHHHHHCCCCCCEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCE
NLTKGEQKRDFIYVEDVANAYATILSKINSFDKKFYDIEVGTGNPVKIKDLVMLIKNLCN
ECCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHHHHCC
SNIKLNFGAIPYRKNEIMNSDANPEFLRNLGWFPKFSLDEGLRKTISYYQKHLVKNDE
CCEEEEECCCCCCHHHHCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SKTILLTGATGFLGSHLLEMLVKEGNNVIILKRSFSNISRIRHLLGDVKYYDIDKVELK
CCEEEEECCHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHCCCEEECCCEEEEE
TPFAENEIDMVIHTATSYGRKNEKISEIVETNLMLPLKLLELCTFFNTDTFFNTDTILPK
CCCCCCCCEEEEEECHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEECCCCCCCC
NLNYYVLSKKQFLEYGKRISDECKLRFINMKLEHMYGPKDDNTKFIPYIIEKMLKNEKEI
CCCEEEECHHHHHHHHHCCCCCCEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCE
NLTKGEQKRDFIYVEDVANAYATILSKINSFDKKFYDIEVGTGNPVKIKDLVMLIKNLCN
ECCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHHHHCC
SNIKLNFGAIPYRKNEIMNSDANPEFLRNLGWFPKFSLDEGLRKTISYYQKHLVKNDE
CCEEEEECCCCCCHHHHCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.019 {NAD+}} 0.034 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: TDP; TTP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1379320 [H]