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The map label for this gene is hycE [C]

Identifier: 150400740

GI number: 150400740

Start: 330267

End: 331391

Strand: Reverse

Name: hycE [C]

Synonym: Maeo_0306

Alternate gene names: 150400740

Gene position: 331391-330267 (Counterclockwise)

Preceding gene: 150400746

Following gene: 150400739

Centisome position: 21.11

GC content: 37.07

Gene sequence:

>1125_bases
ATGTATGAAGGAGAAATTGCAATAGGACCTATTCATTCTGCAATATTGGAACCGCATAGATTAAGATTATTTATAGAAGA
CGAAATTATAAAAGATGCCGAGCTCACCATAGGGGTTAATCATAGAGGCATAGAGCGACTTATGGAAGGACTACCTCCTG
AAAAAGCAACAATCCTAACTGAAAAAATTTGTGGAATATGTTCCCATATACATCTTTGGAGTGCTGTTAGGGTATGTGAG
ATAGGAAGCGATATAGAAGTTCCAGAGCGTGCAAACCACATAAGAGTAATAGTTGAAGAATTAGAAAGATTACACTCACA
TACGCTACTATTTGGGCATGCCTTTGAAATTCTTGGACATGAAACAATGTCAATGAGGGCATTCATGCTAAGAGAGCCAA
TAATGCAAACATTATTTGATATAAGTGGAAGTAGGGTTCAGTATTCATGCCCAATTATAGGAGGAATAAGACCAAGATGC
GATATAAAAGACAGTCAGATTTCAGCATATCTTGAAAGAATGGAAAAATATGAAGATGCATTAAATAAAATAATTGGAAG
GACCATTAACGACCCATTGTTAGTTTCTCGTATGAAAGATATTGGGGTTTTAGATAGGAAAACAGCGGCAAAATACCATG
CTTTGGGACCGACTGCAAGGGGAAGTGGGATAAAAAGCGACATGAGAAAAATGGGCTATGTGCCTGAATATGACTGTTTT
GAGTTCGATGAGATAATTCTTGATGATGGCGATGTATATGCAAGAGTAGCCGTTAGATTCTACGAATGTATTGAAAGTGT
TAAAATAATAAAACAGGCATTAAAGGAGCTCCCAAGTCTCCCTGAAAAATTTTATAATCCAAATTATGAGATAAAGGAAT
TTAAACCGATAGATTGTTATACTGAGGCACAAAGAGGTCAGCAATATTATACATATGGAGTAGATAACGAAGGTAGAGTA
AGACAGGCTAAGGTCAGGACTCCCACAGCTACAAATTTGGCATGTATGGAGGAAGTATTAAAAGATTATCATGTGTCCGA
TGCCGAGCTTATAGTGGCAAGTTGCGACCCCTGTTTTACCTGCACCGATAGATTGATAATATTGCGGGAACAGAATAATA
AATAA

Upstream 100 bases:

>100_bases
ATAATGAAAAATATAATATAAAATTACTTTAATAAAAGAAAATGGAATAAAAAAATATTAATGATAATTAAATTATTAAA
AAAATTAGTAAGGTAATATT

Downstream 100 bases:

>100_bases
CACATAATACGATAACTAAAAAAATAACTAAAAAAAATAATGAAACTATGGCCAATAATTTATTAAAATTATATGTAAAT
TCAACATGCCCACTATGCAA

Product: NADH dehydrogenase (quinone)

Products: NAD+; ubiquinol

Alternate protein names: NA

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MYEGEIAIGPIHSAILEPHRLRLFIEDEIIKDAELTIGVNHRGIERLMEGLPPEKATILTEKICGICSHIHLWSAVRVCE
IGSDIEVPERANHIRVIVEELERLHSHTLLFGHAFEILGHETMSMRAFMLREPIMQTLFDISGSRVQYSCPIIGGIRPRC
DIKDSQISAYLERMEKYEDALNKIIGRTINDPLLVSRMKDIGVLDRKTAAKYHALGPTARGSGIKSDMRKMGYVPEYDCF
EFDEIILDDGDVYARVAVRFYECIESVKIIKQALKELPSLPEKFYNPNYEIKEFKPIDCYTEAQRGQQYYTYGVDNEGRV
RQAKVRTPTATNLACMEEVLKDYHVSDAELIVASCDPCFTCTDRLIILREQNNK

Sequences:

>Translated_374_residues
MYEGEIAIGPIHSAILEPHRLRLFIEDEIIKDAELTIGVNHRGIERLMEGLPPEKATILTEKICGICSHIHLWSAVRVCE
IGSDIEVPERANHIRVIVEELERLHSHTLLFGHAFEILGHETMSMRAFMLREPIMQTLFDISGSRVQYSCPIIGGIRPRC
DIKDSQISAYLERMEKYEDALNKIIGRTINDPLLVSRMKDIGVLDRKTAAKYHALGPTARGSGIKSDMRKMGYVPEYDCF
EFDEIILDDGDVYARVAVRFYECIESVKIIKQALKELPSLPEKFYNPNYEIKEFKPIDCYTEAQRGQQYYTYGVDNEGRV
RQAKVRTPTATNLACMEEVLKDYHVSDAELIVASCDPCFTCTDRLIILREQNNK
>Mature_374_residues
MYEGEIAIGPIHSAILEPHRLRLFIEDEIIKDAELTIGVNHRGIERLMEGLPPEKATILTEKICGICSHIHLWSAVRVCE
IGSDIEVPERANHIRVIVEELERLHSHTLLFGHAFEILGHETMSMRAFMLREPIMQTLFDISGSRVQYSCPIIGGIRPRC
DIKDSQISAYLERMEKYEDALNKIIGRTINDPLLVSRMKDIGVLDRKTAAKYHALGPTARGSGIKSDMRKMGYVPEYDCF
EFDEIILDDGDVYARVAVRFYECIESVKIIKQALKELPSLPEKFYNPNYEIKEFKPIDCYTEAQRGQQYYTYGVDNEGRV
RQAKVRTPTATNLACMEEVLKDYHVSDAELIVASCDPCFTCTDRLIILREQNNK

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG3261

COG function: function code C; Ni,Fe-hydrogenase III large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 49 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI4758786, Length=388, Percent_Identity=26.0309278350515, Blast_Score=102, Evalue=8e-22,
Organism=Homo sapiens, GI260898743, Length=378, Percent_Identity=25.6613756613757, Blast_Score=97, Evalue=3e-20,
Organism=Escherichia coli, GI1789076, Length=371, Percent_Identity=31.5363881401617, Blast_Score=174, Evalue=7e-45,
Organism=Escherichia coli, GI1788832, Length=372, Percent_Identity=31.7204301075269, Blast_Score=174, Evalue=9e-45,
Organism=Escherichia coli, GI145693162, Length=389, Percent_Identity=25.9640102827763, Blast_Score=102, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI17568379, Length=398, Percent_Identity=26.3819095477387, Blast_Score=101, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17555284, Length=395, Percent_Identity=25.5696202531646, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI221459469, Length=384, Percent_Identity=25.5208333333333, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24638644, Length=388, Percent_Identity=26.5463917525773, Blast_Score=115, Evalue=6e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001135
- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00346 Complex1_49kDa; PF00374 NiFeSe_Hases [H]

EC number: 1.6.5.3

Molecular weight: Translated: 42775; Mature: 42775

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS00507 NI_HGENASE_L_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYEGEIAIGPIHSAILEPHRLRLFIEDEIIKDAELTIGVNHRGIERLMEGLPPEKATILT
CCCCCEEECCHHHHHCCCCCEEEEEEHHHCCCCEEEEECCHHHHHHHHHCCCCCHHHHHH
EKICGICSHIHLWSAVRVCEIGSDIEVPERANHIRVIVEELERLHSHTLLFGHAFEILGH
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
ETMSMRAFMLREPIMQTLFDISGSRVQYSCPIIGGIRPRCDIKDSQISAYLERMEKYEDA
HHHHHHHHHHHHHHHHHHHHCCCCEEEEECCEECCCCCCCCCCHHHHHHHHHHHHHHHHH
LNKIIGRTINDPLLVSRMKDIGVLDRKTAAKYHALGPTARGSGIKSDMRKMGYVPEYDCF
HHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCCCC
EFDEIILDDGDVYARVAVRFYECIESVKIIKQALKELPSLPEKFYNPNYEIKEFKPIDCY
CCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCHH
TEAQRGQQYYTYGVDNEGRVRQAKVRTPTATNLACMEEVLKDYHVSDAELIVASCDPCFT
HHHHCCCEEEEECCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCC
CTDRLIILREQNNK
CCCEEEEEEECCCC
>Mature Secondary Structure
MYEGEIAIGPIHSAILEPHRLRLFIEDEIIKDAELTIGVNHRGIERLMEGLPPEKATILT
CCCCCEEECCHHHHHCCCCCEEEEEEHHHCCCCEEEEECCHHHHHHHHHCCCCCHHHHHH
EKICGICSHIHLWSAVRVCEIGSDIEVPERANHIRVIVEELERLHSHTLLFGHAFEILGH
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
ETMSMRAFMLREPIMQTLFDISGSRVQYSCPIIGGIRPRCDIKDSQISAYLERMEKYEDA
HHHHHHHHHHHHHHHHHHHHCCCCEEEEECCEECCCCCCCCCCHHHHHHHHHHHHHHHHH
LNKIIGRTINDPLLVSRMKDIGVLDRKTAAKYHALGPTARGSGIKSDMRKMGYVPEYDCF
HHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCCCC
EFDEIILDDGDVYARVAVRFYECIESVKIIKQALKELPSLPEKFYNPNYEIKEFKPIDCY
CCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCHH
TEAQRGQQYYTYGVDNEGRVRQAKVRTPTATNLACMEEVLKDYHVSDAELIVASCDPCFT
HHHHCCCEEEEECCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCC
CTDRLIILREQNNK
CCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]