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The map label for this gene is purF [H]

Identifier: 150400727

GI number: 150400727

Start: 315488

End: 316867

Strand: Direct

Name: purF [H]

Synonym: Maeo_0292

Alternate gene names: 150400727

Gene position: 315488-316867 (Clockwise)

Preceding gene: 150400726

Following gene: 150400732

Centisome position: 20.1

GC content: 33.62

Gene sequence:

>1380_bases
ATGTGTGGAATTTTTGGAATATATTCGTTTTTAAATAATGATGTTGCAAGTAAAATTTATTATGGCCTTTACGCTTTACA
GCATAGGGGGCAGGAAGGTTCAGGCATAGCTACCTTTGATGGGGAAAAAATTAATTCCTACAAGGGCCTTGGTTTAGTTC
CTGAAATATATACAAATGAAATATTATCGGAGCTCCATGGTTCTGTTGGTATTGGTCATGTTAGATACTCCACAACAGGA
GATAGCTGTATTGAAAATTGTCAGCCATTTGTAGTAAGTAGCTCCATAGGTTCTCTCTCAATAGTCCATAATGGAGATAT
TGTAAATTCCGATAAATTAAAAATTGAGTTAGAAAAATTAGGGCATATTTTTATGTCTTCAACAGATTCAGAAGTTATAG
CCCACCTTTTAGTTAGGGAGCTCCTAAAAACTGACGATATTGTTGAGGCAGTGACAAATATTTCAAAAGAATTAATCGGT
TCATATGCCATTATAATTATGCACAATAATAAACTTATAGCTGTTAGAGGTCCAAACGGATTTAAACCACTTTGTGTAGG
AAAAGATGAAGATGGTTCCTTATATGTTTCCTCTGAAAGCTGTGGATTAGATGTAATTAATGCAAAATTAATAAGAGATG
TGAAACCAGGAGAAATTATAGTAATAGATGGCAATGGTATGGAATCATATAATATGATGCCTGAAAATGAAAAACCAGTG
CCATCAAGCTGTATGTTTGAATATGTTTATTTTGCAAGACCTGATTCCATAATTGATGGAGTTAATGTATATGATGTTAG
GAGAAATATAGGAAAAATACTTGCAAAAGAACACCCTTGCGATGCTGATATTGTTGTCCCAGTTCCTGATTCTGGAATTA
CCTCTGCTATGGGATATTCCGAGGAAGCCAATATTCCATATTATGAAGGCCTAATAAAAAATAGATATGTGGGAAGAACA
TTTATCATACCCTCGCAGGAAGCCAGAGTTTTAGCTGTTAGATTAAAGTTGAATCCCATAAAGCATCTTATTAAAGATAA
AAAAGTGGTTTTAATAGATGATAGTATAGTCAGAGGGACTACTTCACAAAGATTAATCGACTTAGTAAGAAAAGCAGGGG
CAAAAGAAGTTCATTTAAGAGTAGGTTCTCCAAAAATAATTTCACCATGCTTTTATGGTATAGATATGCCAACAAAAGAA
GAATTAATAGGGAGCTCCAAAACGGTTGAAGAAATAAGGGAGCATATAACAGCTGATTCATTAGGTTATTTAAGTATAAA
GGGATTAATAAAGGCCATAGGAAGAAAAGATTTATGTTTTGCCTGCTTAACAGGAAACTATCCTACTGAAGTATCCAATA
AGTTAAACAATGTCAAATAA

Upstream 100 bases:

>100_bases
ACTTTTCGAACATGCCATAATTTTAGAAACATTCATTAATTATAAATATATATTATAGATATTAGAATAATATATATTAA
AAATATTATTAGTGATAACA

Downstream 100 bases:

>100_bases
TATTGCGTAATTTATTTAATTTTCTGATCAATCATAAACAAATATCATAATATATTTAAATATGCACCTATATATTATCA
ATATTTTTATTTACTTCTTT

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MCGIFGIYSFLNNDVASKIYYGLYALQHRGQEGSGIATFDGEKINSYKGLGLVPEIYTNEILSELHGSVGIGHVRYSTTG
DSCIENCQPFVVSSSIGSLSIVHNGDIVNSDKLKIELEKLGHIFMSSTDSEVIAHLLVRELLKTDDIVEAVTNISKELIG
SYAIIIMHNNKLIAVRGPNGFKPLCVGKDEDGSLYVSSESCGLDVINAKLIRDVKPGEIIVIDGNGMESYNMMPENEKPV
PSSCMFEYVYFARPDSIIDGVNVYDVRRNIGKILAKEHPCDADIVVPVPDSGITSAMGYSEEANIPYYEGLIKNRYVGRT
FIIPSQEARVLAVRLKLNPIKHLIKDKKVVLIDDSIVRGTTSQRLIDLVRKAGAKEVHLRVGSPKIISPCFYGIDMPTKE
ELIGSSKTVEEIREHITADSLGYLSIKGLIKAIGRKDLCFACLTGNYPTEVSNKLNNVK

Sequences:

>Translated_459_residues
MCGIFGIYSFLNNDVASKIYYGLYALQHRGQEGSGIATFDGEKINSYKGLGLVPEIYTNEILSELHGSVGIGHVRYSTTG
DSCIENCQPFVVSSSIGSLSIVHNGDIVNSDKLKIELEKLGHIFMSSTDSEVIAHLLVRELLKTDDIVEAVTNISKELIG
SYAIIIMHNNKLIAVRGPNGFKPLCVGKDEDGSLYVSSESCGLDVINAKLIRDVKPGEIIVIDGNGMESYNMMPENEKPV
PSSCMFEYVYFARPDSIIDGVNVYDVRRNIGKILAKEHPCDADIVVPVPDSGITSAMGYSEEANIPYYEGLIKNRYVGRT
FIIPSQEARVLAVRLKLNPIKHLIKDKKVVLIDDSIVRGTTSQRLIDLVRKAGAKEVHLRVGSPKIISPCFYGIDMPTKE
ELIGSSKTVEEIREHITADSLGYLSIKGLIKAIGRKDLCFACLTGNYPTEVSNKLNNVK
>Mature_459_residues
MCGIFGIYSFLNNDVASKIYYGLYALQHRGQEGSGIATFDGEKINSYKGLGLVPEIYTNEILSELHGSVGIGHVRYSTTG
DSCIENCQPFVVSSSIGSLSIVHNGDIVNSDKLKIELEKLGHIFMSSTDSEVIAHLLVRELLKTDDIVEAVTNISKELIG
SYAIIIMHNNKLIAVRGPNGFKPLCVGKDEDGSLYVSSESCGLDVINAKLIRDVKPGEIIVIDGNGMESYNMMPENEKPV
PSSCMFEYVYFARPDSIIDGVNVYDVRRNIGKILAKEHPCDADIVVPVPDSGITSAMGYSEEANIPYYEGLIKNRYVGRT
FIIPSQEARVLAVRLKLNPIKHLIKDKKVVLIDDSIVRGTTSQRLIDLVRKAGAKEVHLRVGSPKIISPCFYGIDMPTKE
ELIGSSKTVEEIREHITADSLGYLSIKGLIKAIGRKDLCFACLTGNYPTEVSNKLNNVK

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=504, Percent_Identity=40.4761904761905, Blast_Score=362, Evalue=1e-100,
Organism=Escherichia coli, GI1788651, Length=453, Percent_Identity=41.5011037527594, Blast_Score=330, Evalue=2e-91,
Organism=Escherichia coli, GI1790167, Length=215, Percent_Identity=33.953488372093, Blast_Score=106, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17554892, Length=485, Percent_Identity=40.8247422680412, Blast_Score=332, Evalue=2e-91,
Organism=Caenorhabditis elegans, GI17539970, Length=222, Percent_Identity=30.6306306306306, Blast_Score=70, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17532897, Length=211, Percent_Identity=28.9099526066351, Blast_Score=68, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17532899, Length=211, Percent_Identity=28.9099526066351, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323958, Length=480, Percent_Identity=37.7083333333333, Blast_Score=291, Evalue=2e-79,
Organism=Saccharomyces cerevisiae, GI6322745, Length=220, Percent_Identity=31.8181818181818, Blast_Score=71, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6323730, Length=205, Percent_Identity=30.2439024390244, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24659598, Length=492, Percent_Identity=40.4471544715447, Blast_Score=367, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24659604, Length=471, Percent_Identity=41.1889596602972, Blast_Score=361, Evalue=1e-100,
Organism=Drosophila melanogaster, GI28573187, Length=496, Percent_Identity=40.3225806451613, Blast_Score=360, Evalue=1e-100,
Organism=Drosophila melanogaster, GI21357745, Length=185, Percent_Identity=30.8108108108108, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 50397; Mature: 50397

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIFGIYSFLNNDVASKIYYGLYALQHRGQEGSGIATFDGEKINSYKGLGLVPEIYTNE
CCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHH
ILSELHGSVGIGHVRYSTTGDSCIENCQPFVVSSSIGSLSIVHNGDIVNSDKLKIELEKL
HHHHHCCCCCCCEEEECCCCHHHHHCCCCEEEECCCCEEEEEECCCEECCCCEEEEHHHH
GHIFMSSTDSEVIAHLLVRELLKTDDIVEAVTNISKELIGSYAIIIMHNNKLIAVRGPNG
CHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCEEEEEEECCEEEEEECCCC
FKPLCVGKDEDGSLYVSSESCGLDVINAKLIRDVKPGEIIVIDGNGMESYNMMPENEKPV
CCCEEECCCCCCCEEEECCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCC
PSSCMFEYVYFARPDSIIDGVNVYDVRRNIGKILAKEHPCDADIVVPVPDSGITSAMGYS
CHHHHHHHHEEECCCHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHCCCC
EEANIPYYEGLIKNRYVGRTFIIPSQEARVLAVRLKLNPIKHLIKDKKVVLIDDSIVRGT
CCCCCCHHHHHHHCCCCCEEEEECCCCCEEEEEEEECCHHHHHHCCCEEEEEECCHHCCC
TSQRLIDLVRKAGAKEVHLRVGSPKIISPCFYGIDMPTKEELIGSSKTVEEIREHITADS
HHHHHHHHHHHCCCCEEEEEECCCCEECCEEECCCCCCHHHHCCCHHHHHHHHHHHCCCC
LGYLSIKGLIKAIGRKDLCFACLTGNYPTEVSNKLNNVK
CCHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHCCCC
>Mature Secondary Structure
MCGIFGIYSFLNNDVASKIYYGLYALQHRGQEGSGIATFDGEKINSYKGLGLVPEIYTNE
CCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHH
ILSELHGSVGIGHVRYSTTGDSCIENCQPFVVSSSIGSLSIVHNGDIVNSDKLKIELEKL
HHHHHCCCCCCCEEEECCCCHHHHHCCCCEEEECCCCEEEEEECCCEECCCCEEEEHHHH
GHIFMSSTDSEVIAHLLVRELLKTDDIVEAVTNISKELIGSYAIIIMHNNKLIAVRGPNG
CHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCEEEEEEECCEEEEEECCCC
FKPLCVGKDEDGSLYVSSESCGLDVINAKLIRDVKPGEIIVIDGNGMESYNMMPENEKPV
CCCEEECCCCCCCEEEECCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCC
PSSCMFEYVYFARPDSIIDGVNVYDVRRNIGKILAKEHPCDADIVVPVPDSGITSAMGYS
CHHHHHHHHEEECCCHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHCCCC
EEANIPYYEGLIKNRYVGRTFIIPSQEARVLAVRLKLNPIKHLIKDKKVVLIDDSIVRGT
CCCCCCHHHHHHHCCCCCEEEEECCCCCEEEEEEEECCHHHHHHCCCEEEEEECCHHCCC
TSQRLIDLVRKAGAKEVHLRVGSPKIISPCFYGIDMPTKEELIGSSKTVEEIREHITADS
HHHHHHHHHHHCCCCEEEEEECCCCEECCEEECCCCCCHHHHCCCHHHHHHHHHHHCCCC
LGYLSIKGLIKAIGRKDLCFACLTGNYPTEVSNKLNNVK
CCHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]