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The map label for this gene is radB [H]

Identifier: 150400720

GI number: 150400720

Start: 308391

End: 309032

Strand: Direct

Name: radB [H]

Synonym: Maeo_0285

Alternate gene names: 150400720

Gene position: 308391-309032 (Clockwise)

Preceding gene: 150400719

Following gene: 150400721

Centisome position: 19.65

GC content: 28.5

Gene sequence:

>642_bases
ATGTTAGAGGATATTTTAAAAGGAGATATTGAAAGAAAAACTATCACCCAAATATATGGTCCTCCGGGTGTTGGAAAAAC
CAATTTATGCATCATTTCTGCTATAAATTTTATAAGGCATGGATATAAAGTTGCATATATTGATACAGAGGGAGGATTAT
CGACCGAAAGAATAAAACAAATTTCAAATAATAATGAAGAATTTAATAATATATTAAATAATTTAATAATATATGAACCT
GAAAGCTTTGAGGAACAATCAACCATACTTAAACAACTTGCCTTAATCGATAATTTGGGACTAATTATTGTAGATGGTAT
TGCATCACTTTATAGGCTAGAACTCTCCGATGATATCAATCAAAATGCAAAAGTAAATAGGATAATGGGAAACCAGATAT
TAATACTAAATAAAATATCAAAAAAGAACAATTCTGCGGTTTTAATTACTAACCAAATAACTGATACCAATAAAGGATTT
AAAGCATCAGGGGGTAAGATTTTAGAATATTGGAGTAAAATAATAATTAGGATAGATAAACCAAACACCATAAGAGAAAT
GACATTAGAAAAACATAGACATGCAGCAGAAGGTCAGAAATTACGATATAAAATAGTCAATAACGGCGTTCATAAATTAT
AG

Upstream 100 bases:

>100_bases
ATCATTCATTATTTAATCGATATTGTTATTATTTCATTATTTATCTTTATTTATTATTTTATTTAATTGTATAGTTATTT
TAAATTTGGTGAATTAAAAT

Downstream 100 bases:

>100_bases
TCATTGTAGGACACCCTGCATAATGCTCTATGTTAAATAAGCGATATTTATAGTTAATCTTCAATGACTGTCCCTTATCC
GCCCTAAATTATTAAATAAA

Product: DNA repair and recombination protein RadB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MLEDILKGDIERKTITQIYGPPGVGKTNLCIISAINFIRHGYKVAYIDTEGGLSTERIKQISNNNEEFNNILNNLIIYEP
ESFEEQSTILKQLALIDNLGLIIVDGIASLYRLELSDDINQNAKVNRIMGNQILILNKISKKNNSAVLITNQITDTNKGF
KASGGKILEYWSKIIIRIDKPNTIREMTLEKHRHAAEGQKLRYKIVNNGVHKL

Sequences:

>Translated_213_residues
MLEDILKGDIERKTITQIYGPPGVGKTNLCIISAINFIRHGYKVAYIDTEGGLSTERIKQISNNNEEFNNILNNLIIYEP
ESFEEQSTILKQLALIDNLGLIIVDGIASLYRLELSDDINQNAKVNRIMGNQILILNKISKKNNSAVLITNQITDTNKGF
KASGGKILEYWSKIIIRIDKPNTIREMTLEKHRHAAEGQKLRYKIVNNGVHKL
>Mature_213_residues
MLEDILKGDIERKTITQIYGPPGVGKTNLCIISAINFIRHGYKVAYIDTEGGLSTERIKQISNNNEEFNNILNNLIIYEP
ESFEEQSTILKQLALIDNLGLIIVDGIASLYRLELSDDINQNAKVNRIMGNQILILNKISKKNNSAVLITNQITDTNKGF
KASGGKILEYWSKIIIRIDKPNTIREMTLEKHRHAAEGQKLRYKIVNNGVHKL

Specific function: Involved in DNA repair and in homologous recombination. May regulate the cleavage reactions of the branch-structured DNA. Has a very weak ATPase activity that is not stimulated by DNA. Binds DNA but does not promote DNA strands exchange [H]

COG id: COG0468

COG function: function code L; RecA/RadA recombinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the eukaryotic recA-like protein family. RadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI46255039, Length=192, Percent_Identity=27.6041666666667, Blast_Score=68, Evalue=7e-12,
Organism=Homo sapiens, GI19924117, Length=187, Percent_Identity=28.3422459893048, Blast_Score=67, Evalue=8e-12,
Organism=Homo sapiens, GI10835029, Length=187, Percent_Identity=28.3422459893048, Blast_Score=67, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6321027, Length=198, Percent_Identity=31.3131313131313, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: 1548 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800-1200 (L-broth) 40,000-60,000 (L-broth + Nalidixate) 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013632
- InterPro:   IPR011939
- InterPro:   IPR020588 [H]

Pfam domain/function: PF08423 Rad51 [H]

EC number: NA

Molecular weight: Translated: 24088; Mature: 24088

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: PS50162 RECA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEDILKGDIERKTITQIYGPPGVGKTNLCIISAINFIRHGYKVAYIDTEGGLSTERIKQ
CCCHHHHCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHH
ISNNNEEFNNILNNLIIYEPESFEEQSTILKQLALIDNLGLIIVDGIASLYRLELSDDIN
HCCCHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHCCCEEEEHHHHHHHEECCCCCCC
QNAKVNRIMGNQILILNKISKKNNSAVLITNQITDTNKGFKASGGKILEYWSKIIIRIDK
CCCEEEEECCCEEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHEEEECC
PNTIREMTLEKHRHAAEGQKLRYKIVNNGVHKL
CCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCC
>Mature Secondary Structure
MLEDILKGDIERKTITQIYGPPGVGKTNLCIISAINFIRHGYKVAYIDTEGGLSTERIKQ
CCCHHHHCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHH
ISNNNEEFNNILNNLIIYEPESFEEQSTILKQLALIDNLGLIIVDGIASLYRLELSDDIN
HCCCHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHCCCEEEEHHHHHHHEECCCCCCC
QNAKVNRIMGNQILILNKISKKNNSAVLITNQITDTNKGFKASGGKILEYWSKIIIRIDK
CCCEEEEECCCEEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHEEEECC
PNTIREMTLEKHRHAAEGQKLRYKIVNNGVHKL
CCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA