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The map label for this gene is cofE [H]

Identifier: 150400644

GI number: 150400644

Start: 220239

End: 221009

Strand: Reverse

Name: cofE [H]

Synonym: Maeo_0206

Alternate gene names: 150400644

Gene position: 221009-220239 (Counterclockwise)

Preceding gene: 150400646

Following gene: 150400643

Centisome position: 14.08

GC content: 33.46

Gene sequence:

>771_bases
ATGGAAAATAAAATAAAGGAAATAAAGGAAATAAAAAAAATGGAAGTAATAGGATTAGAAATTCCAATAATTACAGAGCC
TTTGGGATTAGATTATTTGGTGGATTTAATAGCTCAATATCCATTAAAAGACAACGATATAATTGTAATAGCCGAAACAA
TAATATCAAAACTTGAAGGAAATATATTTAATAAAAAAGATATTGCCCCATCAGAAAAGGCAAAAAAACTTGCCGAACAG
CTTGATAAATGTCCTGAAGTGATGGAAATTATATTAAATGAAAGTAGTGAAATTGTAAAAATGGGCGATAAATTTATAAT
CACAGAAACAAAACATGGATTTGTCTGTGCCAACAGTGGAGTAGATGAAAGTAATTCCAACGATGCCATAAAACCACTAC
CAAAAAATCCAGATAACAGTGCAAAATATCTTAAAAATGCCATCATAGAAAAAACAGGGAAAAAAATTGGAATAATTATA
AATGATAGCATGGGGCGACCATTTAGAAAAGGAGCTTGTGGGGTAGCTATTGGAGTAAGTGGAATTCGTGCTTTATGGGA
TAGAAAGGGGGAGAAAGATTTATTTGGTAGGGAGCTCCATACAACAGAGGTTGGAATAGCCGATGAATTGGCATCAACGG
CATCGGTTGTAATGGGTCAATCTAATGAAGGAATACCACTGGTAATTATAAGGGGAGCTCCTGTTCCATTTACTGAGGGA
GTTGGTAGTGATTTAGTAAGGGAAAAAGAGGAAGATGTTTTTAGAAAATAA

Upstream 100 bases:

>100_bases
TACTATTATAATATTAGTTTATATTAAGTATAAATATTAATAATATATAAAAATATTGTAGGCATAAATATTGTGGAGCT
CCGAGAAAAAGGTAGAAAAT

Downstream 100 bases:

>100_bases
ATTAAATCATAAAATATAAATATAATTTAAAATAAACTATAATGCTAAAAAGTTAATAATAAAAAAAAGTTAAAAAAATT
TAAGTGATGTATAATGTGTT

Product: F420-0--gamma-glutamyl ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MENKIKEIKEIKKMEVIGLEIPIITEPLGLDYLVDLIAQYPLKDNDIIVIAETIISKLEGNIFNKKDIAPSEKAKKLAEQ
LDKCPEVMEIILNESSEIVKMGDKFIITETKHGFVCANSGVDESNSNDAIKPLPKNPDNSAKYLKNAIIEKTGKKIGIII
NDSMGRPFRKGACGVAIGVSGIRALWDRKGEKDLFGRELHTTEVGIADELASTASVVMGQSNEGIPLVIIRGAPVPFTEG
VGSDLVREKEEDVFRK

Sequences:

>Translated_256_residues
MENKIKEIKEIKKMEVIGLEIPIITEPLGLDYLVDLIAQYPLKDNDIIVIAETIISKLEGNIFNKKDIAPSEKAKKLAEQ
LDKCPEVMEIILNESSEIVKMGDKFIITETKHGFVCANSGVDESNSNDAIKPLPKNPDNSAKYLKNAIIEKTGKKIGIII
NDSMGRPFRKGACGVAIGVSGIRALWDRKGEKDLFGRELHTTEVGIADELASTASVVMGQSNEGIPLVIIRGAPVPFTEG
VGSDLVREKEEDVFRK
>Mature_256_residues
MENKIKEIKEIKKMEVIGLEIPIITEPLGLDYLVDLIAQYPLKDNDIIVIAETIISKLEGNIFNKKDIAPSEKAKKLAEQ
LDKCPEVMEIILNESSEIVKMGDKFIITETKHGFVCANSGVDESNSNDAIKPLPKNPDNSAKYLKNAIIEKTGKKIGIII
NDSMGRPFRKGACGVAIGVSGIRALWDRKGEKDLFGRELHTTEVGIADELASTASVVMGQSNEGIPLVIIRGAPVPFTEG
VGSDLVREKEEDVFRK

Specific function: Catalyzes the GTP-dependent successive addition of multiple gamma-linked L-glutamates to the L-lactyl phosphodiester of 7,8-didemethyl-8-hydroxy-5-deazariboflavin (F420-0) to form polyglutamated F420 derivatives [H]

COG id: COG1478

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CofE family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002847
- InterPro:   IPR008225 [H]

Pfam domain/function: PF01996 F420_ligase [H]

EC number: NA

Molecular weight: Translated: 28086; Mature: 28086

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENKIKEIKEIKKMEVIGLEIPIITEPLGLDYLVDLIAQYPLKDNDIIVIAETIISKLEG
CCHHHHHHHHHHHHHHEEEECCEEECCCCHHHHHHHHHHCCCCCCCEEEEHHHHHHHHCC
NIFNKKDIAPSEKAKKLAEQLDKCPEVMEIILNESSEIVKMGDKFIITETKHGFVCANSG
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCEEEEEECCCCEEEECCC
VDESNSNDAIKPLPKNPDNSAKYLKNAIIEKTGKKIGIIINDSMGRPFRKGACGVAIGVS
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCEEEEHHH
GIRALWDRKGEKDLFGRELHTTEVGIADELASTASVVMGQSNEGIPLVIIRGAPVPFTEG
HHHHHHHCCCCHHHCCCHHHHHHCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCC
VGSDLVREKEEDVFRK
CCHHHHHHHHHHHHCC
>Mature Secondary Structure
MENKIKEIKEIKKMEVIGLEIPIITEPLGLDYLVDLIAQYPLKDNDIIVIAETIISKLEG
CCHHHHHHHHHHHHHHEEEECCEEECCCCHHHHHHHHHHCCCCCCCEEEEHHHHHHHHCC
NIFNKKDIAPSEKAKKLAEQLDKCPEVMEIILNESSEIVKMGDKFIITETKHGFVCANSG
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCEEEEEECCCCEEEECCC
VDESNSNDAIKPLPKNPDNSAKYLKNAIIEKTGKKIGIIINDSMGRPFRKGACGVAIGVS
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCEEEEHHH
GIRALWDRKGEKDLFGRELHTTEVGIADELASTASVVMGQSNEGIPLVIIRGAPVPFTEG
HHHHHHHCCCCHHHCCCHHHHHHCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCC
VGSDLVREKEEDVFRK
CCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA