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The map label for this gene is asnB [C]

Identifier: 150400643

GI number: 150400643

Start: 218553

End: 220145

Strand: Reverse

Name: asnB [C]

Synonym: Maeo_0205

Alternate gene names: 150400643

Gene position: 220145-218553 (Counterclockwise)

Preceding gene: 150400644

Following gene: 150400639

Centisome position: 14.03

GC content: 29.63

Gene sequence:

>1593_bases
ATGTGTTCGATAAATGGAATTATATTAAAAGACGACGAGGAAGAGCCGAGTAGTTGTAGCAATGATTATAATTATAATGA
TATACAATTACCAAAACAATTAATAAAAGGAATGAAAATTTTGAAACATAGGGGTCCAGATAGTTCTGGCTTGATGTTTG
ATGATGAGGTAATTTATTTTAATGATTTTAATGAATTGAAAGAGTTAAACACAAGCCAAAAAAATAATAGGTTAGGTATT
GGACACAATAGACTTGCAATAGTGGGAGAAGCTAACCAGCCAATACCTAATGAAAATGAAAATTTATGGACTATTTGCAA
CGGGGAAATATATAACTACAACGAATTAAAAGAAGATTTAAGTAGTCATGACATATATTCAGATACGGACACAGAGATAA
TATTACATGCCTATGAAGAGGACATATTTGATGAATTAGATGGGGATTATGCCTACTGCATATACGATAAGGACGAAAAT
AAAATAATACTTGGTAGGGATTTATTTGGAGTAAAGCCACTTTATTATATTGATAATAAAAAATATTTTGCATTTGCATC
GGAAAAAAAGGCACTAATTAATTTATTGATGGACATAAATAATCTATCTTTTGAAGATGCATACAATTACAACATATCAA
ATTTAAAACCAAATAGTTGTATAGTTTATGATTTAAATGACAATGAGCTTACAAAATGGGAAAATATTAGAAAGATTACT
ACAAATTATTTCAATAAATATAAAAATATGGATTATGATGTCTGTAAAGAAGAACTTGAAGAGGCACTATGGAGCTCCGT
GTCAAAACGAGTTAATGGAATTGATGAAATTGGAATAATTTATTCAGGGGGAGTAGATAGCACACTTATAGCAAAAATGG
CATCAGAATACGGAGAAGTAATATTGTATGTAGTGGGAATTGATGAAAAAAGCGATGACATAATATGGGCTGAAAAAGCT
GCAAATGATATGGGGCTAAAATTAAGAAAAAAAATAATAAACCCCAAAGATTATGAAAACTATCTTTTAAAAGTTGCCTA
TGCCATTGATGAAATTGATTTAATGAAAATGGCTGTTGGTATCCCTATGTTTGTAGCTTCGGAAATGGCAAAAGAGGACG
GCATAAAAGTTGTATTATCTGGACAAGGTGCAGACGAATTATTTGGGGGATACAATAGATATAAAAGAATATTTGATGAA
AACGGGGCAAATGGATTAAAAGAGGCATTATATAATGATGTAATGGATATATATAAAGTCAATTTGGAGAGAGATGACCA
CTGCACAATGGCAAATGGTGTGGAGCTCCGTGTTCCATTTTTAGATAAAAATGTTGTTGAGCTAGGATTATCAATTCCTA
TCGAATATAAATTAAATGAAAAAGAGAGAAAAATAATTTTAAGAGATATAGCTTTAAAATATATTCCCGAATATATCGCC
TATCGTCCAAAAAAAGCTGCCCAATATGGTAGTGGAAGCGAAAAAATAATATTTAAAGTTGCTAAAAATTATGGATATTC
TAAAAAAGAAATTAATAAATTCTTTGACGAAATATTATTTAAGAAATTAAAGGGGATGTATATTAATAAATAA

Upstream 100 bases:

>100_bases
AAAATAAATTAAATCATAAAATATAAATATAATTTAAAATAAACTATAATGCTAAAAAGTTAATAATAAAAAAAAGTTAA
AAAAATTTAAGTGATGTATA

Downstream 100 bases:

>100_bases
AAATATAAATTCGGTAAAAGGTGCAATATTGAAAAGAATTGTTGAACTGGATTATCTTAGGTGTATTGCAATATGGGAGT
TATTGCTATCCATATAAGTC

Product: asparagine synthase (glutamine-hydrolyzing)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 530; Mature: 530

Protein sequence:

>530_residues
MCSINGIILKDDEEEPSSCSNDYNYNDIQLPKQLIKGMKILKHRGPDSSGLMFDDEVIYFNDFNELKELNTSQKNNRLGI
GHNRLAIVGEANQPIPNENENLWTICNGEIYNYNELKEDLSSHDIYSDTDTEIILHAYEEDIFDELDGDYAYCIYDKDEN
KIILGRDLFGVKPLYYIDNKKYFAFASEKKALINLLMDINNLSFEDAYNYNISNLKPNSCIVYDLNDNELTKWENIRKIT
TNYFNKYKNMDYDVCKEELEEALWSSVSKRVNGIDEIGIIYSGGVDSTLIAKMASEYGEVILYVVGIDEKSDDIIWAEKA
ANDMGLKLRKKIINPKDYENYLLKVAYAIDEIDLMKMAVGIPMFVASEMAKEDGIKVVLSGQGADELFGGYNRYKRIFDE
NGANGLKEALYNDVMDIYKVNLERDDHCTMANGVELRVPFLDKNVVELGLSIPIEYKLNEKERKIILRDIALKYIPEYIA
YRPKKAAQYGSGSEKIIFKVAKNYGYSKKEINKFFDEILFKKLKGMYINK

Sequences:

>Translated_530_residues
MCSINGIILKDDEEEPSSCSNDYNYNDIQLPKQLIKGMKILKHRGPDSSGLMFDDEVIYFNDFNELKELNTSQKNNRLGI
GHNRLAIVGEANQPIPNENENLWTICNGEIYNYNELKEDLSSHDIYSDTDTEIILHAYEEDIFDELDGDYAYCIYDKDEN
KIILGRDLFGVKPLYYIDNKKYFAFASEKKALINLLMDINNLSFEDAYNYNISNLKPNSCIVYDLNDNELTKWENIRKIT
TNYFNKYKNMDYDVCKEELEEALWSSVSKRVNGIDEIGIIYSGGVDSTLIAKMASEYGEVILYVVGIDEKSDDIIWAEKA
ANDMGLKLRKKIINPKDYENYLLKVAYAIDEIDLMKMAVGIPMFVASEMAKEDGIKVVLSGQGADELFGGYNRYKRIFDE
NGANGLKEALYNDVMDIYKVNLERDDHCTMANGVELRVPFLDKNVVELGLSIPIEYKLNEKERKIILRDIALKYIPEYIA
YRPKKAAQYGSGSEKIIFKVAKNYGYSKKEINKFFDEILFKKLKGMYINK
>Mature_530_residues
MCSINGIILKDDEEEPSSCSNDYNYNDIQLPKQLIKGMKILKHRGPDSSGLMFDDEVIYFNDFNELKELNTSQKNNRLGI
GHNRLAIVGEANQPIPNENENLWTICNGEIYNYNELKEDLSSHDIYSDTDTEIILHAYEEDIFDELDGDYAYCIYDKDEN
KIILGRDLFGVKPLYYIDNKKYFAFASEKKALINLLMDINNLSFEDAYNYNISNLKPNSCIVYDLNDNELTKWENIRKIT
TNYFNKYKNMDYDVCKEELEEALWSSVSKRVNGIDEIGIIYSGGVDSTLIAKMASEYGEVILYVVGIDEKSDDIIWAEKA
ANDMGLKLRKKIINPKDYENYLLKVAYAIDEIDLMKMAVGIPMFVASEMAKEDGIKVVLSGQGADELFGGYNRYKRIFDE
NGANGLKEALYNDVMDIYKVNLERDDHCTMANGVELRVPFLDKNVVELGLSIPIEYKLNEKERKIILRDIALKYIPEYIA
YRPKKAAQYGSGSEKIIFKVAKNYGYSKKEINKFFDEILFKKLKGMYINK

Specific function: Asparagine biosynthesis. [C]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=507, Percent_Identity=28.0078895463511, Blast_Score=152, Evalue=1e-36,
Organism=Homo sapiens, GI168229252, Length=507, Percent_Identity=28.0078895463511, Blast_Score=152, Evalue=1e-36,
Organism=Homo sapiens, GI168229250, Length=507, Percent_Identity=28.0078895463511, Blast_Score=152, Evalue=1e-36,
Organism=Homo sapiens, GI296010848, Length=495, Percent_Identity=28.0808080808081, Blast_Score=149, Evalue=8e-36,
Organism=Homo sapiens, GI296010852, Length=403, Percent_Identity=27.7915632754342, Blast_Score=129, Evalue=5e-30,
Organism=Homo sapiens, GI296010850, Length=403, Percent_Identity=27.7915632754342, Blast_Score=129, Evalue=5e-30,
Organism=Homo sapiens, GI9506697, Length=144, Percent_Identity=38.1944444444444, Blast_Score=82, Evalue=1e-15,
Organism=Escherichia coli, GI1786889, Length=522, Percent_Identity=27.0114942528736, Blast_Score=174, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI25147557, Length=516, Percent_Identity=28.4883720930233, Blast_Score=166, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI71993933, Length=516, Percent_Identity=28.4883720930233, Blast_Score=165, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI17560178, Length=469, Percent_Identity=28.5714285714286, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI25147560, Length=295, Percent_Identity=28.8135593220339, Blast_Score=92, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6321563, Length=519, Percent_Identity=28.9017341040462, Blast_Score=158, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6325403, Length=550, Percent_Identity=27.4545454545455, Blast_Score=157, Evalue=5e-39,
Organism=Saccharomyces cerevisiae, GI6323542, Length=412, Percent_Identity=28.1553398058252, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI45553209, Length=534, Percent_Identity=26.7790262172285, Blast_Score=155, Evalue=7e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 61068; Mature: 61068

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCSINGIILKDDEEEPSSCSNDYNYNDIQLPKQLIKGMKILKHRGPDSSGLMFDDEVIYF
CCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEECCEEEE
NDFNELKELNTSQKNNRLGIGHNRLAIVGEANQPIPNENENLWTICNGEIYNYNELKEDL
CCHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCEEEEECCCEECHHHHHHHH
SSHDIYSDTDTEIILHAYEEDIFDELDGDYAYCIYDKDENKIILGRDLFGVKPLYYIDNK
HCCCCCCCCCCEEEEEHHHHHHHHHCCCCEEEEEEECCCCEEEEECCCCCCCCEEEECCC
KYFAFASEKKALINLLMDINNLSFEDAYNYNISNLKPNSCIVYDLNDNELTKWENIRKIT
EEEEEECHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHH
TNYFNKYKNMDYDVCKEELEEALWSSVSKRVNGIDEIGIIYSGGVDSTLIAKMASEYGEV
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHCCCCE
ILYVVGIDEKSDDIIWAEKAANDMGLKLRKKIINPKDYENYLLKVAYAIDEIDLMKMAVG
EEEEEECCCCCCCEEEEHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
IPMFVASEMAKEDGIKVVLSGQGADELFGGYNRYKRIFDENGANGLKEALYNDVMDIYKV
CHHHHHHHHHHCCCCEEEEECCCHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHEE
NLERDDHCTMANGVELRVPFLDKNVVELGLSIPIEYKLNEKERKIILRDIALKYIPEYIA
CCCCCCCCEEECCCEEEEECCCCCHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHH
YRPKKAAQYGSGSEKIIFKVAKNYGYSKKEINKFFDEILFKKLKGMYINK
CCCHHHHCCCCCCCEEEEEEHHHCCCCHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MCSINGIILKDDEEEPSSCSNDYNYNDIQLPKQLIKGMKILKHRGPDSSGLMFDDEVIYF
CCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEECCEEEE
NDFNELKELNTSQKNNRLGIGHNRLAIVGEANQPIPNENENLWTICNGEIYNYNELKEDL
CCHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCEEEEECCCEECHHHHHHHH
SSHDIYSDTDTEIILHAYEEDIFDELDGDYAYCIYDKDENKIILGRDLFGVKPLYYIDNK
HCCCCCCCCCCEEEEEHHHHHHHHHCCCCEEEEEEECCCCEEEEECCCCCCCCEEEECCC
KYFAFASEKKALINLLMDINNLSFEDAYNYNISNLKPNSCIVYDLNDNELTKWENIRKIT
EEEEEECHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHH
TNYFNKYKNMDYDVCKEELEEALWSSVSKRVNGIDEIGIIYSGGVDSTLIAKMASEYGEV
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHCCCCE
ILYVVGIDEKSDDIIWAEKAANDMGLKLRKKIINPKDYENYLLKVAYAIDEIDLMKMAVG
EEEEEECCCCCCCEEEEHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
IPMFVASEMAKEDGIKVVLSGQGADELFGGYNRYKRIFDENGANGLKEALYNDVMDIYKV
CHHHHHHHHHHCCCCEEEEECCCHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHEE
NLERDDHCTMANGVELRVPFLDKNVVELGLSIPIEYKLNEKERKIILRDIALKYIPEYIA
CCCCCCCCEEECCCEEEEECCCCCHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHH
YRPKKAAQYGSGSEKIIFKVAKNYGYSKKEINKFFDEILFKKLKGMYINK
CCCHHHHCCCCCCCEEEEEEHHHCCCCHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]