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The map label for this gene is bioB

Identifier: 150400621

GI number: 150400621

Start: 201028

End: 202005

Strand: Direct

Name: bioB

Synonym: Maeo_0183

Alternate gene names: 150400621

Gene position: 201028-202005 (Clockwise)

Preceding gene: 150400620

Following gene: 150400622

Centisome position: 12.81

GC content: 28.12

Gene sequence:

>978_bases
ATGGAAGAATTTACAGGTATAGGCTATTTTTACAAAAAATCCATTAAAAACCAGTTGGATAAAAAGGATGCTTCGTTATT
ATGGGAGCTCCCCTATTACACATTATTGTATCTTGCAAATTGTGTAAAAAAACACAATAATAGTAATAATATCGATTTAT
GTTCCATAATAAATGCCAAATCTGGATTATGTTCTGAAAATTGTGCTTTTTGTTCTCAATCATTACATAACAGCTCAAAA
ATAAAAGAATATCCTTTAAAACCAAAAGAAGATATTTTAAAACAGGCGAAATACATAGAAAAATATAGTAATAGATTTTC
AATAGTTGTTAGTGGTAAAACAGTAAATGATAGAGAATATAATGAAATAATAGATGCTATAAAGGACATCAAAAAAGAAA
CCAATTTAAAAGTTTGTGCTTCTTTGGGATTATTGGATAATAGTCAATTAAAAGAATTAAAGGATTTAGATGTTAGAATA
CACAATAATTTAGAAACTTCAAAAGAATATTTTGATAAAATATGCACCACCCACACATATGATGACAAAGTAAAATCCAT
AACAATGGCAAAAAAAATAGGTTTGGAGGTCTGTAGTGGTGGCATAATTGGACTAGGGGAAAGCCTTGAAAATAGAATAA
ATTTATTTTATGAATTAAAAGAATTGGATGTTGAGGGAATACCCATAAATATATACAATCCAATTAAAGGAACTAAAACC
TATGAATTATTAAATAACAATGCGATAAAACAAATTACTCCAATTGAAGCTTTAAAATCCATTGCCATATGTAAATTAAT
ATTGCCTAATAAAGATGTTAGATTATGTGGTGGCAGAGAATACAATTTGAGAGATTGGCAAAGTTTATCATTATTGGCAA
TTGATGGGCTTATGATTGGCAACTATTTAACCACCAATGGTAGAAATATCGAAGACGATATAAAAATGATAGTTGACGGG
GGATTTGATGGAAAATAA

Upstream 100 bases:

>100_bases
CTTCTTGATTTAACTGGAAAAGCCCATAAATACGGTGCTTAAATTTAATTTATTTAAATTAAATATTATTATTTTATTAT
TTTATAATATGTGTGATATT

Downstream 100 bases:

>100_bases
TATATTAAAAAATACAATAGATGATGAGTTAAAGAAAAAATACAATTTATTAAAAGAATATTTTAAAGATAAAACTGTAA
TTATATCATATTCGGGGGGA

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MEEFTGIGYFYKKSIKNQLDKKDASLLWELPYYTLLYLANCVKKHNNSNNIDLCSIINAKSGLCSENCAFCSQSLHNSSK
IKEYPLKPKEDILKQAKYIEKYSNRFSIVVSGKTVNDREYNEIIDAIKDIKKETNLKVCASLGLLDNSQLKELKDLDVRI
HNNLETSKEYFDKICTTHTYDDKVKSITMAKKIGLEVCSGGIIGLGESLENRINLFYELKELDVEGIPINIYNPIKGTKT
YELLNNNAIKQITPIEALKSIAICKLILPNKDVRLCGGREYNLRDWQSLSLLAIDGLMIGNYLTTNGRNIEDDIKMIVDG
GFDGK

Sequences:

>Translated_325_residues
MEEFTGIGYFYKKSIKNQLDKKDASLLWELPYYTLLYLANCVKKHNNSNNIDLCSIINAKSGLCSENCAFCSQSLHNSSK
IKEYPLKPKEDILKQAKYIEKYSNRFSIVVSGKTVNDREYNEIIDAIKDIKKETNLKVCASLGLLDNSQLKELKDLDVRI
HNNLETSKEYFDKICTTHTYDDKVKSITMAKKIGLEVCSGGIIGLGESLENRINLFYELKELDVEGIPINIYNPIKGTKT
YELLNNNAIKQITPIEALKSIAICKLILPNKDVRLCGGREYNLRDWQSLSLLAIDGLMIGNYLTTNGRNIEDDIKMIVDG
GFDGK
>Mature_325_residues
MEEFTGIGYFYKKSIKNQLDKKDASLLWELPYYTLLYLANCVKKHNNSNNIDLCSIINAKSGLCSENCAFCSQSLHNSSK
IKEYPLKPKEDILKQAKYIEKYSNRFSIVVSGKTVNDREYNEIIDAIKDIKKETNLKVCASLGLLDNSQLKELKDLDVRI
HNNLETSKEYFDKICTTHTYDDKVKSITMAKKIGLEVCSGGIIGLGESLENRINLFYELKELDVEGIPINIYNPIKGTKT
YELLNNNAIKQITPIEALKSIAICKLILPNKDVRLCGGREYNLRDWQSLSLLAIDGLMIGNYLTTNGRNIEDDIKMIVDG
GFDGK

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=306, Percent_Identity=28.4313725490196, Blast_Score=139, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6321725, Length=314, Percent_Identity=29.9363057324841, Blast_Score=155, Evalue=7e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_META3 (A6UTF3)

Other databases:

- EMBL:   CP000743
- RefSeq:   YP_001324387.1
- ProteinModelPortal:   A6UTF3
- SMR:   A6UTF3
- STRING:   A6UTF3
- GeneID:   5326588
- GenomeReviews:   CP000743_GR
- KEGG:   mae:Maeo_0183
- eggNOG:   arNOG05930
- HOGENOM:   HBG405569
- OMA:   MGMETCM
- BioCyc:   MAEO419665:MAEO_0183-MONOMER
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 36835; Mature: 36835

Theoretical pI: Translated: 7.61; Mature: 7.61

Prosite motif: PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEFTGIGYFYKKSIKNQLDKKDASLLWELPYYTLLYLANCVKKHNNSNNIDLCSIINAK
CCCCCCCHHHHHHHHHHHHCCCCHHHEEHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCC
SGLCSENCAFCSQSLHNSSKIKEYPLKPKEDILKQAKYIEKYSNRFSIVVSGKTVNDREY
CCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHH
NEIIDAIKDIKKETNLKVCASLGLLDNSQLKELKDLDVRIHNNLETSKEYFDKICTTHTY
HHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHCCEEEECCCHHHHHHHHHHHCCCCC
DDKVKSITMAKKIGLEVCSGGIIGLGESLENRINLFYELKELDVEGIPINIYNPIKGTKT
HHHHHHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHEEHHHCCCCCCEEEEECCCCCCCH
YELLNNNAIKQITPIEALKSIAICKLILPNKDVRLCGGREYNLRDWQSLSLLAIDGLMIG
HHHHCCCCCHHCCHHHHHHHHHHHHEECCCCCEEEECCCCCCCCCCCCEEEEEECCEEEC
NYLTTNGRNIEDDIKMIVDGGFDGK
CEEECCCCCCHHHHHEEEECCCCCC
>Mature Secondary Structure
MEEFTGIGYFYKKSIKNQLDKKDASLLWELPYYTLLYLANCVKKHNNSNNIDLCSIINAK
CCCCCCCHHHHHHHHHHHHCCCCHHHEEHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCC
SGLCSENCAFCSQSLHNSSKIKEYPLKPKEDILKQAKYIEKYSNRFSIVVSGKTVNDREY
CCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHH
NEIIDAIKDIKKETNLKVCASLGLLDNSQLKELKDLDVRIHNNLETSKEYFDKICTTHTY
HHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHCCEEEECCCHHHHHHHHHHHCCCCC
DDKVKSITMAKKIGLEVCSGGIIGLGESLENRINLFYELKELDVEGIPINIYNPIKGTKT
HHHHHHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHEEHHHCCCCCCEEEEECCCCCCCH
YELLNNNAIKQITPIEALKSIAICKLILPNKDVRLCGGREYNLRDWQSLSLLAIDGLMIG
HHHHCCCCCHHCCHHHHHHHHHHHHEECCCCCEEEECCCCCCCCCCCCEEEEEECCEEEC
NYLTTNGRNIEDDIKMIVDGGFDGK
CEEECCCCCCHHHHHEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA