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The map label for this gene is cysU [H]

Identifier: 150400355

GI number: 150400355

Start: 1634857

End: 1635657

Strand: Reverse

Name: cysU [H]

Synonym: Mevan_1618

Alternate gene names: 150400355

Gene position: 1635657-1634857 (Counterclockwise)

Preceding gene: 150400356

Following gene: 150400354

Centisome position: 95.09

GC content: 30.46

Gene sequence:

>801_bases
ATGAAAAACAATGATTTTTTAAAGTTTTTTTCATTTTCCTCACTTCTTATTTTCAGTTTATTGATAATTTCAATTATAAT
GGCAATTATTTCAAATATAACTGCTGAAACACTTTTTTCAGCAATATTATCCAAAGAAATACACTTTGCAATAAAATTAA
GTGTATTAACTGCAAGTATTGCAACAATTTTTGGCGGTATTTTGGGCATCCCTACAGCATATGCACTTGCAAGATATGAA
TTTAAGGGGAAATTCCTAATAGATAGCCTTGTAGAACTTCCAATAATTCTTCCACCGTTAATTACCGGTTTTGCACTTTT
GGTATTTTTTGGAAGTACTGAATTTGGAAGATTTATTTCTGAAAATATCATTGATGTTTTATTTACACCAAAGGGAATCG
TTGTTGCACAATTTTTTGTTGCAACGCCATTTATAGTTAGGACTTCAAAGTCGGTTTTTGAAAGCATTGATGAAAAATAT
GAACTAATTGCACAAAGCCTTGGGTCAAAACGTCATGAAAGTTTTATAAATGTAATACTTCCAATGGCAAAAAATGGAAT
ATTTACGGGCCTAATTTTGGCTTGGGCAAGGAGTATCGGAGAATTTGGTGCAACAGTAATGCTTGCAGGGGCTACAAAAA
TGAAAACAGAAACTCTTCCAATAGCGGTTTTTCTAAACATTTCTCTTGGAAACTTTGAAAAGGCACTTGCTGTTTCATTT
ATATTTTTAGTTGTTGCAATAACAATATTAAGTATTCTAAAGATGATTTTTAATAATGGTGAAAAAAATGATAGATTTTA
A

Upstream 100 bases:

>100_bases
CTATGATAGTTCAAAATTATTCTACGATTACGTAAATAATGAAGGAAAAGCTATATTCAAAAAACATGGATTTATGGTAA
TTGAATAGTTGGGGCGTATT

Downstream 100 bases:

>100_bases
ATTAGAATTGGAAAAAATTGCAAAAGAAAATAACTTTTTAAACGAAACTGTCGAAATAAAACCTATTAATATAGATTTAA
AATCAAAAACAATTAATGAT

Product: NifC-like ABC-type transporter

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MKNNDFLKFFSFSSLLIFSLLIISIIMAIISNITAETLFSAILSKEIHFAIKLSVLTASIATIFGGILGIPTAYALARYE
FKGKFLIDSLVELPIILPPLITGFALLVFFGSTEFGRFISENIIDVLFTPKGIVVAQFFVATPFIVRTSKSVFESIDEKY
ELIAQSLGSKRHESFINVILPMAKNGIFTGLILAWARSIGEFGATVMLAGATKMKTETLPIAVFLNISLGNFEKALAVSF
IFLVVAITILSILKMIFNNGEKNDRF

Sequences:

>Translated_266_residues
MKNNDFLKFFSFSSLLIFSLLIISIIMAIISNITAETLFSAILSKEIHFAIKLSVLTASIATIFGGILGIPTAYALARYE
FKGKFLIDSLVELPIILPPLITGFALLVFFGSTEFGRFISENIIDVLFTPKGIVVAQFFVATPFIVRTSKSVFESIDEKY
ELIAQSLGSKRHESFINVILPMAKNGIFTGLILAWARSIGEFGATVMLAGATKMKTETLPIAVFLNISLGNFEKALAVSF
IFLVVAITILSILKMIFNNGEKNDRF
>Mature_266_residues
MKNNDFLKFFSFSSLLIFSLLIISIIMAIISNITAETLFSAILSKEIHFAIKLSVLTASIATIFGGILGIPTAYALARYE
FKGKFLIDSLVELPIILPPLITGFALLVFFGSTEFGRFISENIIDVLFTPKGIVVAQFFVATPFIVRTSKSVFESIDEKY
ELIAQSLGSKRHESFINVILPMAKNGIFTGLILAWARSIGEFGATVMLAGATKMKTETLPIAVFLNISLGNFEKALAVSF
IFLVVAITILSILKMIFNNGEKNDRF

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788764, Length=211, Percent_Identity=33.6492890995261, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI87082099, Length=227, Percent_Identity=29.0748898678414, Blast_Score=100, Evalue=8e-23,
Organism=Escherichia coli, GI1786980, Length=217, Percent_Identity=34.5622119815668, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1787369, Length=204, Percent_Identity=26.9607843137255, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011865
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 29259; Mature: 29259

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNNDFLKFFSFSSLLIFSLLIISIIMAIISNITAETLFSAILSKEIHFAIKLSVLTASI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATIFGGILGIPTAYALARYEFKGKFLIDSLVELPIILPPLITGFALLVFFGSTEFGRFIS
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
ENIIDVLFTPKGIVVAQFFVATPFIVRTSKSVFESIDEKYELIAQSLGSKRHESFINVIL
HHHHHHHCCCCHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
PMAKNGIFTGLILAWARSIGEFGATVMLAGATKMKTETLPIAVFLNISLGNFEKALAVSF
HHHCCCHHHHHHHHHHHHHHHHCCCEEEECCHHHCCCCCEEEEEEEECCCCHHHHHHHHH
IFLVVAITILSILKMIFNNGEKNDRF
HHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKNNDFLKFFSFSSLLIFSLLIISIIMAIISNITAETLFSAILSKEIHFAIKLSVLTASI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATIFGGILGIPTAYALARYEFKGKFLIDSLVELPIILPPLITGFALLVFFGSTEFGRFIS
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
ENIIDVLFTPKGIVVAQFFVATPFIVRTSKSVFESIDEKYELIAQSLGSKRHESFINVIL
HHHHHHHCCCCHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
PMAKNGIFTGLILAWARSIGEFGATVMLAGATKMKTETLPIAVFLNISLGNFEKALAVSF
HHHCCCHHHHHHHHHHHHHHHHCCCEEEECCHHHCCCCCEEEEEEEECCCCHHHHHHHHH
IFLVVAITILSILKMIFNNGEKNDRF
HHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2188958; 9205837; 9278503; 2188959 [H]