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The map label for this gene is wecC

Identifier: 150400321

GI number: 150400321

Start: 1599409

End: 1600692

Strand: Reverse

Name: wecC

Synonym: Mevan_1584

Alternate gene names: 150400321

Gene position: 1600692-1599409 (Counterclockwise)

Preceding gene: 150400322

Following gene: 150400320

Centisome position: 93.06

GC content: 32.17

Gene sequence:

>1284_bases
ATGGATAAACACGAAAAGCAAAAAATTAAGAAAATTTGTGTGATAGGGCTTGGATATATAGGACTTCCAACTGCATCAAT
GCTTGCAAACCACGGTTACGAAGTAATTGGGGTAGATATTAGTGAAAAAAGAGTAAACGAAATTAAAAATGGGGACTTTA
AAATTGAAGAACCAGGGCTCTTAACTCTTTTAAAAGGAGCAATTAATTCAAAAAATCTAAATGTAAAAACTAAAGCAGAA
AAAGCCGATGCATTTATTATTTGCGTTCCAACCCCTGCAATAGGTTGCGATGACGGTTCAAAAAAATGCGATTTAAGCTA
TGTTTTAGATGCTGTTAATTCAATCTTACCATATATTGATGAAGGAAACCTGATTGTAATTGAAAGCACAATCCCACCTG
AAACAACTCAAAAAATTTATGATATCATTGATAAAAAAGTATATGTTGCCCACTGCCCTGAAAGAGTTCTTCCCGGAAAA
ATATTAAAAGAACTCGTTGAAAATGACCGGATAATCGGTGGAATTAATAAAAAATCTGCGGAAATGGCAAAAGAAATTTA
TAAATCTTTTGTAGAAGGGAAAATTTACATAACTGATTCAAATACTGCGGAAATGGTAAAATTAATGGAAAATACGTATC
GAGATATTAATATCGCTCTTGCAAATGAATTTGCAAAAATCTGCGATGAAATAGGTGTAAATGTATGGGATGCGATAAAA
ATTGCAAATAAGCACCCTAGAGTAAATATTTTAAACCCTGGCCCGGGGGTTGGAGGGCATTGCATAAGTATTGACCCATG
GTTTATTGTTGAAAAAACCAATAATGCAAAATTTATTCGATCAGCAAGGGAATTGAATGACAAAATGCCTTATTACGTGT
GTAATATGATAATATCCGAATTAAAGAATTTAAACATTGAAAAACCAAAAGTTACCGTATTTGGTGCAACATACAAGGGA
AATGTTGAAGATACTAGAGAAAGTCCCTCAAAAAAAGTTATAGATGCACTTGCTGAAAAAAATATTCCAGTTTCAACTTA
TGACCCTCATGCGAATTCTTTTGAATACGAACTACACTCCCTTGAAGATAGCATTGTTAATTCAGACTGTATTGTAGTAT
TAACTGACCATAATGAATTTAAAAGCTTTAAAAAGGAAGAAATAGATGAAATATCTAAAAAACTTAAAAATAAGCTCATA
ATTGACACTAAGAATATATTAAATCATAACCTTTGGAAAAAAGCAGGATTTAAAATAAAACTGCTCGGTAATGGGGCTTG
GTGA

Upstream 100 bases:

>100_bases
ACTTGTTGCAACGTTCCTTACAATATTTTTAAGTGTCGTAGTTGAGGCTTCGTGGGCATACCCATTAGCAAACAAACTTT
TTAAATAATTTGGTGATTTC

Downstream 100 bases:

>100_bases
CTAAATGTTAAAAATAGGGATAATCCTTGGAACTCGACCCGAGATAATTAAAATGTCCCCCTTAATTCGGGGACTACAAA
ATAAAAATTCTTTTTTAATT

Product: UDP-glucose/GDP-mannose dehydrogenase

Products: UDP-N-acetyl-D-mannosaminuronic-acid; NADH; Proton [C]

Alternate protein names: UDP-ManNAc 6-dehydrogenase

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MDKHEKQKIKKICVIGLGYIGLPTASMLANHGYEVIGVDISEKRVNEIKNGDFKIEEPGLLTLLKGAINSKNLNVKTKAE
KADAFIICVPTPAIGCDDGSKKCDLSYVLDAVNSILPYIDEGNLIVIESTIPPETTQKIYDIIDKKVYVAHCPERVLPGK
ILKELVENDRIIGGINKKSAEMAKEIYKSFVEGKIYITDSNTAEMVKLMENTYRDINIALANEFAKICDEIGVNVWDAIK
IANKHPRVNILNPGPGVGGHCISIDPWFIVEKTNNAKFIRSARELNDKMPYYVCNMIISELKNLNIEKPKVTVFGATYKG
NVEDTRESPSKKVIDALAEKNIPVSTYDPHANSFEYELHSLEDSIVNSDCIVVLTDHNEFKSFKKEEIDEISKKLKNKLI
IDTKNILNHNLWKKAGFKIKLLGNGAW

Sequences:

>Translated_427_residues
MDKHEKQKIKKICVIGLGYIGLPTASMLANHGYEVIGVDISEKRVNEIKNGDFKIEEPGLLTLLKGAINSKNLNVKTKAE
KADAFIICVPTPAIGCDDGSKKCDLSYVLDAVNSILPYIDEGNLIVIESTIPPETTQKIYDIIDKKVYVAHCPERVLPGK
ILKELVENDRIIGGINKKSAEMAKEIYKSFVEGKIYITDSNTAEMVKLMENTYRDINIALANEFAKICDEIGVNVWDAIK
IANKHPRVNILNPGPGVGGHCISIDPWFIVEKTNNAKFIRSARELNDKMPYYVCNMIISELKNLNIEKPKVTVFGATYKG
NVEDTRESPSKKVIDALAEKNIPVSTYDPHANSFEYELHSLEDSIVNSDCIVVLTDHNEFKSFKKEEIDEISKKLKNKLI
IDTKNILNHNLWKKAGFKIKLLGNGAW
>Mature_427_residues
MDKHEKQKIKKICVIGLGYIGLPTASMLANHGYEVIGVDISEKRVNEIKNGDFKIEEPGLLTLLKGAINSKNLNVKTKAE
KADAFIICVPTPAIGCDDGSKKCDLSYVLDAVNSILPYIDEGNLIVIESTIPPETTQKIYDIIDKKVYVAHCPERVLPGK
ILKELVENDRIIGGINKKSAEMAKEIYKSFVEGKIYITDSNTAEMVKLMENTYRDINIALANEFAKICDEIGVNVWDAIK
IANKHPRVNILNPGPGVGGHCISIDPWFIVEKTNNAKFIRSARELNDKMPYYVCNMIISELKNLNIEKPKVTVFGATYKG
NVEDTRESPSKKVIDALAEKNIPVSTYDPHANSFEYELHSLEDSIVNSDCIVVLTDHNEFKSFKKEEIDEISKKLKNKLI
IDTKNILNHNLWKKAGFKIKLLGNGAW

Specific function: Catalyzes the four-electron oxidation of UDP-N-acetyl-D- mannosamine (UDP-ManNAc), reducing NAD(+) and releasing UDP-N- acetylmannosaminuronic acid (UDP-ManNAcA)

COG id: COG0677

COG function: function code M; UDP-N-acetyl-D-mannosaminuronate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UDP-glucose/GDP-mannose dehydrogenase family

Homologues:

Organism=Homo sapiens, GI4507813, Length=466, Percent_Identity=27.0386266094421, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI296040443, Length=454, Percent_Identity=24.8898678414097, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI296040438, Length=365, Percent_Identity=24.1095890410959, Blast_Score=81, Evalue=2e-15,
Organism=Escherichia coli, GI48994968, Length=424, Percent_Identity=38.4433962264151, Blast_Score=322, Evalue=4e-89,
Organism=Escherichia coli, GI1788340, Length=406, Percent_Identity=24.6305418719212, Blast_Score=78, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17560350, Length=465, Percent_Identity=26.6666666666667, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI17136908, Length=460, Percent_Identity=25, Blast_Score=125, Evalue=8e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): WECC_METVS (A6USK4)

Other databases:

- EMBL:   CP000742
- RefSeq:   YP_001324088.1
- ProteinModelPortal:   A6USK4
- SMR:   A6USK4
- STRING:   A6USK4
- GeneID:   5324798
- GenomeReviews:   CP000742_GR
- KEGG:   mvn:Mevan_1584
- eggNOG:   arNOG05577
- HOGENOM:   HBG400967
- OMA:   ANHGYEV
- ProtClustDB:   CLSK876185
- InterPro:   IPR008927
- InterPro:   IPR016040
- InterPro:   IPR017476
- InterPro:   IPR014027
- InterPro:   IPR014026
- InterPro:   IPR014028
- InterPro:   IPR001732
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR11374
- PIRSF:   PIRSF000124
- TIGRFAMs:   TIGR03026

Pfam domain/function: PF00984 UDPG_MGDP_dh; PF03720 UDPG_MGDP_dh_C; PF03721 UDPG_MGDP_dh_N; SSF48179 6DGDH_C_like; SSF52413 UDP-Glc/GDP-Man_DH_C

EC number: 1.1.1.- [C]

Molecular weight: Translated: 47792; Mature: 47792

Theoretical pI: Translated: 7.21; Mature: 7.21

Prosite motif: NA

Important sites: ACT_SITE 261-261

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKHEKQKIKKICVIGLGYIGLPTASMLANHGYEVIGVDISEKRVNEIKNGDFKIEEPGL
CCCHHHHHHHHHHHHCCCHHCCCHHHHHHHCCCEEEEECCCHHHHHHHCCCCEEECCCCH
LTLLKGAINSKNLNVKTKAEKADAFIICVPTPAIGCDDGSKKCDLSYVLDAVNSILPYID
HHHHHHCCCCCCCCEEEECCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCEEC
EGNLIVIESTIPPETTQKIYDIIDKKVYVAHCPERVLPGKILKELVENDRIIGGINKKSA
CCCEEEEECCCCCHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHCCCEEECCCHHHH
EMAKEIYKSFVEGKIYITDSNTAEMVKLMENTYRDINIALANEFAKICDEIGVNVWDAIK
HHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHCCCHHHHHE
IANKHPRVNILNPGPGVGGHCISIDPWFIVEKTNNAKFIRSARELNDKMPYYVCNMIISE
ECCCCCEEEEECCCCCCCCEEEEECCEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHH
LKNLNIEKPKVTVFGATYKGNVEDTRESPSKKVIDALAEKNIPVSTYDPHANSFEYELHS
HHCCCCCCCEEEEEEEEEECCCCHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHH
LEDSIVNSDCIVVLTDHNEFKSFKKEEIDEISKKLKNKLIIDTKNILNHNLWKKAGFKIK
HHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCEEEECHHHHHHHHHHHCCCEEE
LLGNGAW
EEECCCC
>Mature Secondary Structure
MDKHEKQKIKKICVIGLGYIGLPTASMLANHGYEVIGVDISEKRVNEIKNGDFKIEEPGL
CCCHHHHHHHHHHHHCCCHHCCCHHHHHHHCCCEEEEECCCHHHHHHHCCCCEEECCCCH
LTLLKGAINSKNLNVKTKAEKADAFIICVPTPAIGCDDGSKKCDLSYVLDAVNSILPYID
HHHHHHCCCCCCCCEEEECCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCEEC
EGNLIVIESTIPPETTQKIYDIIDKKVYVAHCPERVLPGKILKELVENDRIIGGINKKSA
CCCEEEEECCCCCHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHCCCEEECCCHHHH
EMAKEIYKSFVEGKIYITDSNTAEMVKLMENTYRDINIALANEFAKICDEIGVNVWDAIK
HHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHCCCHHHHHE
IANKHPRVNILNPGPGVGGHCISIDPWFIVEKTNNAKFIRSARELNDKMPYYVCNMIISE
ECCCCCEEEEECCCCCCCCEEEEECCEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHH
LKNLNIEKPKVTVFGATYKGNVEDTRESPSKKVIDALAEKNIPVSTYDPHANSFEYELHS
HHCCCCCCCEEEEEEEEEECCCCHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHH
LEDSIVNSDCIVVLTDHNEFKSFKKEEIDEISKKLKNKLIIDTKNILNHNLWKKAGFKIK
HHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCEEEECHHHHHHHHHHHCCCEEE
LLGNGAW
EEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-N-acetyl-D-mannosamine; NAD [C]

Specific reaction: UDP-N-acetyl-D-mannosamine + NAD = UDP-N-acetyl-D-mannosaminuronic-acid + NADH + Proton [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA