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The map label for this gene is truD

Identifier: 150400227

GI number: 150400227

Start: 1510118

End: 1511290

Strand: Reverse

Name: truD

Synonym: Mevan_1488

Alternate gene names: 150400227

Gene position: 1511290-1510118 (Counterclockwise)

Preceding gene: 150400233

Following gene: 150400224

Centisome position: 87.86

GC content: 31.03

Gene sequence:

>1173_bases
ATGCCGTTAAATATTGAAAAATATATACTTAAAACGAATAGATTTGGGGGAACTTTAAAAAAATATCCTGAAGACTTTAT
TGTAGAAGAAATAATGCCTGATGGAACAGTTTTAGAAGTTGGAAAAGAAATTGATTTTTTAGATGAAACTCCTTGGAACG
GTTCATTTATCCATTTTACCCTTGAAAAACGAAATTGGAACACTATGGATGCATTAAGTGCAATTGTAAGGGCGACTAAA
ACTAAACGGAAAAATTTTGGATTTGCTGGAACAAAAGACAAATTTGCAGTAACTACTCAGAGAATGGGATGTTTTGGGCT
TAAAAAAGAACAGCTTGAAAGCGTTAAAATTCCTGAAATAATAATAAAAGATATTCAAAAGTCTAATAAAAAGCTTAGAA
TGGGGGACCTCTTTGGAAACCGGTTTACGATAAACATAAGAGATATTGAAAAAAAATATATGGAATTAGAAAACCTTTCA
GATCTTAAACTTGACCATGTTTTAAATTATTTTGGAATACAGCGTTTTGGGCTTAAAAGGCCTATTACCCATATAGTTGG
AAAATTTATATATGAGAGAGATTTTGAAAGTGCATTTTACACATACTGCGGAACCCCAATAAGTGAAACGGGTGATGAAA
AGGAGGCACGGGAACTTGTTGATTCAGGAAATTTTAAGGGCGCATTAAAACTATTTTCCAAAGGAAATGAATATGAAAAA
AGATTAATTCAACAGTATTTTAAATATAAGGACTTTAAAATGGCGTTTACCGCACTTCCTCCGCAATTAAATAGTATGTT
TGTAAATGCTTACCAAGCATATCTTTTTAATGAAATGGTAAATGAAAGGTATAACTTTGGATTTGAACCACTTACGGGAG
ATATTTTAGAAGATAATATACCAACTGGGGCATTAATTGGTTACAATACAGAATTTGGTAAAGGAATTCAGGGCGAAATT
GAAAAAGAAATATTTAATCGTGAAAACATTGACTTAAAAAAATTTAAAATAGAAGATTTTGGAAATTTCTATGGGACAAG
ACGAAAATTAATTACCCCAGTTTACGACTTTAAAAGCGAATTTAAGGAAAAAGTGCTTACTTTAAGCTTTAAATTAGAGC
GGGGAAATTATGCAACGATTGTAACTAGGGAATTTACTGGAAATTTAGGTTAA

Upstream 100 bases:

>100_bases
GGAAGCAAGAAAAAAAGAAACTAATGATATTATGTCGGATTTTAGAAAAAATATATCCTTAAAATTTAGAAAAAATAAAA
TAGATGGTAAACTAAAAGAA

Downstream 100 bases:

>100_bases
AATAACTATATATAATAACAAGACTAATTTATTTTGCAGTTGATTTTAATGATACTGTATTTAGCAGTTCTACCTACAAG
GCGGCCTTTTTGAGTATCCG

Product: tRNA pseudouridine synthase D TruD

Products: NA

Alternate protein names: tRNA pseudouridylate synthase D; tRNA-uridine isomerase D

Number of amino acids: Translated: 390; Mature: 389

Protein sequence:

>390_residues
MPLNIEKYILKTNRFGGTLKKYPEDFIVEEIMPDGTVLEVGKEIDFLDETPWNGSFIHFTLEKRNWNTMDALSAIVRATK
TKRKNFGFAGTKDKFAVTTQRMGCFGLKKEQLESVKIPEIIIKDIQKSNKKLRMGDLFGNRFTINIRDIEKKYMELENLS
DLKLDHVLNYFGIQRFGLKRPITHIVGKFIYERDFESAFYTYCGTPISETGDEKEARELVDSGNFKGALKLFSKGNEYEK
RLIQQYFKYKDFKMAFTALPPQLNSMFVNAYQAYLFNEMVNERYNFGFEPLTGDILEDNIPTGALIGYNTEFGKGIQGEI
EKEIFNRENIDLKKFKIEDFGNFYGTRRKLITPVYDFKSEFKEKVLTLSFKLERGNYATIVTREFTGNLG

Sequences:

>Translated_390_residues
MPLNIEKYILKTNRFGGTLKKYPEDFIVEEIMPDGTVLEVGKEIDFLDETPWNGSFIHFTLEKRNWNTMDALSAIVRATK
TKRKNFGFAGTKDKFAVTTQRMGCFGLKKEQLESVKIPEIIIKDIQKSNKKLRMGDLFGNRFTINIRDIEKKYMELENLS
DLKLDHVLNYFGIQRFGLKRPITHIVGKFIYERDFESAFYTYCGTPISETGDEKEARELVDSGNFKGALKLFSKGNEYEK
RLIQQYFKYKDFKMAFTALPPQLNSMFVNAYQAYLFNEMVNERYNFGFEPLTGDILEDNIPTGALIGYNTEFGKGIQGEI
EKEIFNRENIDLKKFKIEDFGNFYGTRRKLITPVYDFKSEFKEKVLTLSFKLERGNYATIVTREFTGNLG
>Mature_389_residues
PLNIEKYILKTNRFGGTLKKYPEDFIVEEIMPDGTVLEVGKEIDFLDETPWNGSFIHFTLEKRNWNTMDALSAIVRATKT
KRKNFGFAGTKDKFAVTTQRMGCFGLKKEQLESVKIPEIIIKDIQKSNKKLRMGDLFGNRFTINIRDIEKKYMELENLSD
LKLDHVLNYFGIQRFGLKRPITHIVGKFIYERDFESAFYTYCGTPISETGDEKEARELVDSGNFKGALKLFSKGNEYEKR
LIQQYFKYKDFKMAFTALPPQLNSMFVNAYQAYLFNEMVNERYNFGFEPLTGDILEDNIPTGALIGYNTEFGKGIQGEIE
KEIFNRENIDLKKFKIEDFGNFYGTRRKLITPVYDFKSEFKEKVLTLSFKLERGNYATIVTREFTGNLG

Specific function: Could be responsible for synthesis of pseudouridine from uracil-13 in transfer RNAs

COG id: COG0585

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRUD domain

Homologues:

Organism=Homo sapiens, GI50727002, Length=391, Percent_Identity=25.5754475703325, Blast_Score=115, Evalue=9e-26,
Organism=Homo sapiens, GI148762985, Length=396, Percent_Identity=22.7272727272727, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI148762961, Length=396, Percent_Identity=22.7272727272727, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI148762948, Length=396, Percent_Identity=22.7272727272727, Blast_Score=88, Evalue=2e-17,
Organism=Escherichia coli, GI1789102, Length=167, Percent_Identity=31.7365269461078, Blast_Score=72, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17557232, Length=428, Percent_Identity=23.3644859813084, Blast_Score=85, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6324817, Length=310, Percent_Identity=27.0967741935484, Blast_Score=110, Evalue=4e-25,
Organism=Drosophila melanogaster, GI21355799, Length=255, Percent_Identity=27.843137254902, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUD_METVS (A6USB0)

Other databases:

- EMBL:   CP000742
- RefSeq:   YP_001323994.1
- ProteinModelPortal:   A6USB0
- SMR:   A6USB0
- STRING:   A6USB0
- GeneID:   5325302
- GenomeReviews:   CP000742_GR
- KEGG:   mvn:Mevan_1488
- eggNOG:   arNOG05422
- HOGENOM:   HBG541221
- OMA:   WAGTKDK
- ProtClustDB:   CLSK876256
- HAMAP:   MF_01082
- InterPro:   IPR020103
- InterPro:   IPR001656
- InterPro:   IPR011760
- PANTHER:   PTHR13326
- TIGRFAMs:   TIGR00094

Pfam domain/function: PF01142 TruD; SSF55120 PsdUridine_synth_cat_dom

EC number: NA

Molecular weight: Translated: 45379; Mature: 45247

Theoretical pI: Translated: 8.98; Mature: 8.98

Prosite motif: PS50984 TRUD; PS01268 UPF0024

Important sites: ACT_SITE 93-93

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLNIEKYILKTNRFGGTLKKYPEDFIVEEIMPDGTVLEVGKEIDFLDETPWNGSFIHFT
CCCCHHHHHEECCCCCCCHHHCCHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCEEEEE
LEKRNWNTMDALSAIVRATKTKRKNFGFAGTKDKFAVTTQRMGCFGLKKEQLESVKIPEI
EECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHCCCCCCHHHHHHCCCHHH
IIKDIQKSNKKLRMGDLFGNRFTINIRDIEKKYMELENLSDLKLDHVLNYFGIQRFGLKR
HHHHHHHCCCCEEEHEECCCEEEEEEHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCC
PITHIVGKFIYERDFESAFYTYCGTPISETGDEKEARELVDSGNFKGALKLFSKGNEYEK
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHCCCHHHH
RLIQQYFKYKDFKMAFTALPPQLNSMFVNAYQAYLFNEMVNERYNFGFEPLTGDILEDNI
HHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCC
PTGALIGYNTEFGKGIQGEIEKEIFNRENIDLKKFKIEDFGNFYGTRRKLITPVYDFKSE
CCCEEEECCCCCCCCCCHHHHHHHHCCCCCCEEEEEHHHHHHHHHCHHHHHCHHHHHHHH
FKEKVLTLSFKLERGNYATIVTREFTGNLG
HHHHHEEEEEEEECCCEEEEEEEECCCCCC
>Mature Secondary Structure 
PLNIEKYILKTNRFGGTLKKYPEDFIVEEIMPDGTVLEVGKEIDFLDETPWNGSFIHFT
CCCHHHHHEECCCCCCCHHHCCHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCEEEEE
LEKRNWNTMDALSAIVRATKTKRKNFGFAGTKDKFAVTTQRMGCFGLKKEQLESVKIPEI
EECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHCCCCCCHHHHHHCCCHHH
IIKDIQKSNKKLRMGDLFGNRFTINIRDIEKKYMELENLSDLKLDHVLNYFGIQRFGLKR
HHHHHHHCCCCEEEHEECCCEEEEEEHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCC
PITHIVGKFIYERDFESAFYTYCGTPISETGDEKEARELVDSGNFKGALKLFSKGNEYEK
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHCCCHHHH
RLIQQYFKYKDFKMAFTALPPQLNSMFVNAYQAYLFNEMVNERYNFGFEPLTGDILEDNI
HHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCC
PTGALIGYNTEFGKGIQGEIEKEIFNRENIDLKKFKIEDFGNFYGTRRKLITPVYDFKSE
CCCEEEECCCCCCCCCCHHHHHHHHCCCCCCEEEEEHHHHHHHHHCHHHHHCHHHHHHHH
FKEKVLTLSFKLERGNYATIVTREFTGNLG
HHHHHEEEEEEEECCCEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA