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The map label for this gene is thrS

Identifier: 150400144

GI number: 150400144

Start: 1435117

End: 1436985

Strand: Reverse

Name: thrS

Synonym: Mevan_1403

Alternate gene names: 150400144

Gene position: 1436985-1435117 (Counterclockwise)

Preceding gene: 150400152

Following gene: 150400141

Centisome position: 83.54

GC content: 34.3

Gene sequence:

>1869_bases
ATGAAAACACTACTTATACATTCTGATTATTTGGAATTTACTGCTAAAGAAAAAACCAAAATTGCAGAGGATGCAGAAAT
TTTAAACGGAAAAATGGATGAATGTTTAACCGTTTTTATTGCCGTTGAAAAGGAAGACGAGTCTGACCCTGTTGCAGTAA
TTAAAAATGCAGTTTTTGAAATAATAAAAACTGCTGGAAACTTGAATGTTAAAAACATTGTAGTTTATCCTTATGCGCAC
CTTTCAAGTGATTTAGGATCCCCAGAAACTGCAAAAGAAATTTTAAAAGGAATTGAAAAGGAACTTTCAAGCTCTTTTGA
AGTACTTAGGGCCCCATTTGGATGGTATAAATCATTTAAAATTAGCTGTAAAGGCCATCCATTAAGTGAACTTTCAAGAA
AGATCGGTACTGAAAGGATTGAAAAGGAGAAAAAGGAAAAGGTTGTTTCAAAATTTTACATAATAAATGGGGAAAATTCA
GAACTTACCGAAGTAAATGATGAATTACTCTCTAAACTAAAAGATAAAGGACTTTTAGCACTTTTAAAACACGAACTCGA
TATTAAAGAAGAAGGAAAAGAAAATGGGGAACCCCCTCATGTAAAATACATTAAAGAAAAAGAAATTTGTGATTATGAAC
CTAGTTCTGATGCGGGTCACTTTAGATGGTATCCTAAAGGAAAATTAATAAGAGATTTGCTCTCTGATTACGTTTATAAC
CTTGTTGTAGAAAATGGCGGAATGCCTGTTGAAACACCTGTTATGTACGACCTTCAAAATAATGCAATTAGGGAACATGC
AGATAAATTTGGAGAAAGACAATACCGGTTTAGGCAAGGAAATAAGGACTTAATGTTAAGATTTGCGGCATGTTTTGGAC
AGTTCATGATGAAAAAAGACATGTACCTTCTTCCAAAACATCTTCCTTTAAAATTGTATGAACTTTCAACATACAGTTTT
AGGTACGAACAGAGGGGGGAGTTAGTTGGTCTAAAAAGACTAAGAGGATTTACAATGCCTGACATGCATACGGTATGTAT
CGACATGAATCAGGCCATGGAAGCATTTGAAAACCAGTTTTGGATGGGATTAAAAACAGGAGATGACTTTAAAACCCCAT
ACTCTATCATATTTAGATTTACAAATGAGTTCTTTGAAGAAAATAAAGAATGGTTCTTCAAAATGGCGAAAGAATATAAA
CTAAAATACGGAAAAGATGCAATTCTAGAAATTTTACCGGGTAGAAAGCATTATTGGGTCGGAAAAGTTGATATGGCAGT
TGTTGATAGTTTCGGTAGGCCAATTGAAAATCCAACCGTTCAAATTGACGTTGAGAGTGCACAAAGATTTGGAATTACGG
TTCATGATGGCGACAAAACAATTCACCCAATAATACTACACTGTTCCCCAACAGGAAGTATTGAAAGAGTGTTATGCGGC
CTTTTGGAAAATGCGTATATTAAAACTTTAGAAGATAAACCACCTGCACTTCCTACATGGTTAACCCCAATTCAGGCAAG
AGTTATTCCAGTTTCCGAAAGAAATGAAGAATTTGCTCTTGAAGTAGTGAATAAATTAAGAGAATCCGGTATCAGGACAG
ACTTTGACGATAGGGAAGACAGCATGGGTAAAAAAATAAGAAATGCAGGAACAGATTGGGTAAATTACGTCGTTGTAATT
GGAGATAATGAAATGGAAAGTAAAAAGTTAACTGTTACATTAAGAGAAGAATCTTCAATAAAACAGCCCAAAAAAGAATC
TTTAACTGTTGGAGAACTGATTGAAAAAGTAACTTCTGAAATTAAAGGATCACCCAATAGGCCACTTCCACTTCCAATGA
AGTGTTCAGTACAGCCAATATTTAGATAA

Upstream 100 bases:

>100_bases
CCTTATTAAACATATATATTTATGTAGAATTTATGTAATTTTTAATTCGATATAGTTTGATAAAAGAGTTTAATACAAGA
ATTAAATATAGAGGGAAATA

Downstream 100 bases:

>100_bases
CCGTAACTCTTTTAAATTTTTAATTTTTTATCGGGTTAAAAATCTTTTTTAAAAGTAAAATAAAATTAAAATAAAAAAAT
AGGAATTTATTCTATTTTGA

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS

Number of amino acids: Translated: 622; Mature: 622

Protein sequence:

>622_residues
MKTLLIHSDYLEFTAKEKTKIAEDAEILNGKMDECLTVFIAVEKEDESDPVAVIKNAVFEIIKTAGNLNVKNIVVYPYAH
LSSDLGSPETAKEILKGIEKELSSSFEVLRAPFGWYKSFKISCKGHPLSELSRKIGTERIEKEKKEKVVSKFYIINGENS
ELTEVNDELLSKLKDKGLLALLKHELDIKEEGKENGEPPHVKYIKEKEICDYEPSSDAGHFRWYPKGKLIRDLLSDYVYN
LVVENGGMPVETPVMYDLQNNAIREHADKFGERQYRFRQGNKDLMLRFAACFGQFMMKKDMYLLPKHLPLKLYELSTYSF
RYEQRGELVGLKRLRGFTMPDMHTVCIDMNQAMEAFENQFWMGLKTGDDFKTPYSIIFRFTNEFFEENKEWFFKMAKEYK
LKYGKDAILEILPGRKHYWVGKVDMAVVDSFGRPIENPTVQIDVESAQRFGITVHDGDKTIHPIILHCSPTGSIERVLCG
LLENAYIKTLEDKPPALPTWLTPIQARVIPVSERNEEFALEVVNKLRESGIRTDFDDREDSMGKKIRNAGTDWVNYVVVI
GDNEMESKKLTVTLREESSIKQPKKESLTVGELIEKVTSEIKGSPNRPLPLPMKCSVQPIFR

Sequences:

>Translated_622_residues
MKTLLIHSDYLEFTAKEKTKIAEDAEILNGKMDECLTVFIAVEKEDESDPVAVIKNAVFEIIKTAGNLNVKNIVVYPYAH
LSSDLGSPETAKEILKGIEKELSSSFEVLRAPFGWYKSFKISCKGHPLSELSRKIGTERIEKEKKEKVVSKFYIINGENS
ELTEVNDELLSKLKDKGLLALLKHELDIKEEGKENGEPPHVKYIKEKEICDYEPSSDAGHFRWYPKGKLIRDLLSDYVYN
LVVENGGMPVETPVMYDLQNNAIREHADKFGERQYRFRQGNKDLMLRFAACFGQFMMKKDMYLLPKHLPLKLYELSTYSF
RYEQRGELVGLKRLRGFTMPDMHTVCIDMNQAMEAFENQFWMGLKTGDDFKTPYSIIFRFTNEFFEENKEWFFKMAKEYK
LKYGKDAILEILPGRKHYWVGKVDMAVVDSFGRPIENPTVQIDVESAQRFGITVHDGDKTIHPIILHCSPTGSIERVLCG
LLENAYIKTLEDKPPALPTWLTPIQARVIPVSERNEEFALEVVNKLRESGIRTDFDDREDSMGKKIRNAGTDWVNYVVVI
GDNEMESKKLTVTLREESSIKQPKKESLTVGELIEKVTSEIKGSPNRPLPLPMKCSVQPIFR
>Mature_622_residues
MKTLLIHSDYLEFTAKEKTKIAEDAEILNGKMDECLTVFIAVEKEDESDPVAVIKNAVFEIIKTAGNLNVKNIVVYPYAH
LSSDLGSPETAKEILKGIEKELSSSFEVLRAPFGWYKSFKISCKGHPLSELSRKIGTERIEKEKKEKVVSKFYIINGENS
ELTEVNDELLSKLKDKGLLALLKHELDIKEEGKENGEPPHVKYIKEKEICDYEPSSDAGHFRWYPKGKLIRDLLSDYVYN
LVVENGGMPVETPVMYDLQNNAIREHADKFGERQYRFRQGNKDLMLRFAACFGQFMMKKDMYLLPKHLPLKLYELSTYSF
RYEQRGELVGLKRLRGFTMPDMHTVCIDMNQAMEAFENQFWMGLKTGDDFKTPYSIIFRFTNEFFEENKEWFFKMAKEYK
LKYGKDAILEILPGRKHYWVGKVDMAVVDSFGRPIENPTVQIDVESAQRFGITVHDGDKTIHPIILHCSPTGSIERVLCG
LLENAYIKTLEDKPPALPTWLTPIQARVIPVSERNEEFALEVVNKLRESGIRTDFDDREDSMGKKIRNAGTDWVNYVVVI
GDNEMESKKLTVTLREESSIKQPKKESLTVGELIEKVTSEIKGSPNRPLPLPMKCSVQPIFR

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI38570111, Length=384, Percent_Identity=27.34375, Blast_Score=124, Evalue=3e-28,
Organism=Homo sapiens, GI38202255, Length=414, Percent_Identity=26.5700483091787, Blast_Score=122, Evalue=9e-28,
Organism=Homo sapiens, GI20070344, Length=407, Percent_Identity=23.5872235872236, Blast_Score=101, Evalue=2e-21,
Organism=Escherichia coli, GI1788013, Length=399, Percent_Identity=24.812030075188, Blast_Score=107, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71984192, Length=399, Percent_Identity=25.8145363408521, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI71984184, Length=399, Percent_Identity=25.8145363408521, Blast_Score=117, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6322112, Length=412, Percent_Identity=26.2135922330097, Blast_Score=119, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6322654, Length=319, Percent_Identity=26.6457680250784, Blast_Score=91, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24583839, Length=423, Percent_Identity=25.0591016548463, Blast_Score=123, Evalue=4e-28,
Organism=Drosophila melanogaster, GI24583842, Length=423, Percent_Identity=25.0591016548463, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI28574141, Length=423, Percent_Identity=25.0591016548463, Blast_Score=122, Evalue=5e-28,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYT_METVS (A6US27)

Other databases:

- EMBL:   CP000742
- RefSeq:   YP_001323911.1
- ProteinModelPortal:   A6US27
- SMR:   A6US27
- STRING:   A6US27
- GeneID:   5325134
- GenomeReviews:   CP000742_GR
- KEGG:   mvn:Mevan_1403
- eggNOG:   arNOG04477
- HOGENOM:   HBG392335
- OMA:   ETPVMYD
- ProtClustDB:   PRK03991
- GO:   GO:0005737
- HAMAP:   MF_00184_A
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002320
- InterPro:   IPR015011
- Gene3D:   G3DSA:3.40.50.800
- PRINTS:   PR01047
- ProDom:   PD016189
- TIGRFAMs:   TIGR00418

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF08915 tRNA-Thr_ED; SSF52954 Anticodon_bd

EC number: =6.1.1.3

Molecular weight: Translated: 71495; Mature: 71495

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTLLIHSDYLEFTAKEKTKIAEDAEILNGKMDECLTVFIAVEKEDESDPVAVIKNAVFE
CCEEEEECHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEEEEEECCCCCCHHHHHHHHHHH
IIKTAGNLNVKNIVVYPYAHLSSDLGSPETAKEILKGIEKELSSSFEVLRAPFGWYKSFK
HHHHCCCCCCCEEEEEECHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
ISCKGHPLSELSRKIGTERIEKEKKEKVVSKFYIINGENSELTEVNDELLSKLKDKGLLA
EEECCCCHHHHHHHHCHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHCCCHHH
LLKHELDIKEEGKENGEPPHVKYIKEKEICDYEPSSDAGHFRWYPKGKLIRDLLSDYVYN
HHHHHCCCHHCCCCCCCCCCEEEECCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHE
LVVENGGMPVETPVMYDLQNNAIREHADKFGERQYRFRQGNKDLMLRFAACFGQFMMKKD
EEEECCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCC
MYLLPKHLPLKLYELSTYSFRYEQRGELVGLKRLRGFTMPDMHTVCIDMNQAMEAFENQF
CCCCCCCCCEEEEEECEEEEEHHHCCCEEEHHHHCCCCCCCCCEEEEEHHHHHHHHHHCE
WMGLKTGDDFKTPYSIIFRFTNEFFEENKEWFFKMAKEYKLKYGKDAILEILPGRKHYWV
EEEEECCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHCCCCCEEEE
GKVDMAVVDSFGRPIENPTVQIDVESAQRFGITVHDGDKTIHPIILHCSPTGSIERVLCG
CCHHHHHHHHCCCCCCCCEEEEEECCCHHCCEEEECCCCEEEEEEEEECCCCCHHHHHHH
LLENAYIKTLEDKPPALPTWLTPIQARVIPVSERNEEFALEVVNKLRESGIRTDFDDRED
HHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCHH
SMGKKIRNAGTDWVNYVVVIGDNEMESKKLTVTLREESSIKQPKKESLTVGELIEKVTSE
HHHHHHHHCCCCEEEEEEEECCCCCCCCEEEEEEECCCCCCCCCHHCCCHHHHHHHHHHH
IKGSPNRPLPLPMKCSVQPIFR
HCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKTLLIHSDYLEFTAKEKTKIAEDAEILNGKMDECLTVFIAVEKEDESDPVAVIKNAVFE
CCEEEEECHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEEEEEECCCCCCHHHHHHHHHHH
IIKTAGNLNVKNIVVYPYAHLSSDLGSPETAKEILKGIEKELSSSFEVLRAPFGWYKSFK
HHHHCCCCCCCEEEEEECHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
ISCKGHPLSELSRKIGTERIEKEKKEKVVSKFYIINGENSELTEVNDELLSKLKDKGLLA
EEECCCCHHHHHHHHCHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHCCCHHH
LLKHELDIKEEGKENGEPPHVKYIKEKEICDYEPSSDAGHFRWYPKGKLIRDLLSDYVYN
HHHHHCCCHHCCCCCCCCCCEEEECCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHE
LVVENGGMPVETPVMYDLQNNAIREHADKFGERQYRFRQGNKDLMLRFAACFGQFMMKKD
EEEECCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCC
MYLLPKHLPLKLYELSTYSFRYEQRGELVGLKRLRGFTMPDMHTVCIDMNQAMEAFENQF
CCCCCCCCCEEEEEECEEEEEHHHCCCEEEHHHHCCCCCCCCCEEEEEHHHHHHHHHHCE
WMGLKTGDDFKTPYSIIFRFTNEFFEENKEWFFKMAKEYKLKYGKDAILEILPGRKHYWV
EEEEECCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHCCCCCEEEE
GKVDMAVVDSFGRPIENPTVQIDVESAQRFGITVHDGDKTIHPIILHCSPTGSIERVLCG
CCHHHHHHHHCCCCCCCCEEEEEECCCHHCCEEEECCCCEEEEEEEEECCCCCHHHHHHH
LLENAYIKTLEDKPPALPTWLTPIQARVIPVSERNEEFALEVVNKLRESGIRTDFDDRED
HHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCHH
SMGKKIRNAGTDWVNYVVVIGDNEMESKKLTVTLREESSIKQPKKESLTVGELIEKVTSE
HHHHHHHHCCCCEEEEEEEECCCCCCCCEEEEEEECCCCCCCCCHHCCCHHHHHHHHHHH
IKGSPNRPLPLPMKCSVQPIFR
HCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA