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The map label for this gene is nifH2 [H]

Identifier: 150399896

GI number: 150399896

Start: 1166538

End: 1167416

Strand: Reverse

Name: nifH2 [H]

Synonym: Mevan_1152

Alternate gene names: 150399896

Gene position: 1167416-1166538 (Counterclockwise)

Preceding gene: 150399897

Following gene: 150399895

Centisome position: 67.87

GC content: 33.9

Gene sequence:

>879_bases
TTGAAACAGATTGCATTTTACGGAAAAGGAGGGATTGGAAAGTCAACTACAGTATGTAACTTAGCCGCAGCACTGTCAAA
AAGCGGAAAAAAGGTTATTGTCGTTGGTTGTGATCCAAAACATGACTGTACATCAAATTTAAGAGGTGGAATAGAAATTC
CAACCGTTTTAGACATTTTAAGAGAAAAAGGAATAGATACGTTGGGAATAGATACAATAATTCATGAAAATTTGCTTAAA
AAAGAAGATATACTTTACAAAGGATTTAATGGCATATACTGCGTTGAAGCTGGGGGCCCTAAACCCGGATACGGTTGTGC
AGGGCGAGGAGTTATTGTAGTTATTGATTTACTTAAAAAAATGAATGTTTTTAAAGATTTAGGCGTTGATATTGTTCTTT
ATGACGTTTTAGGAGATGTTGTTTGTGGAGGATTTGCAATGCCCTTAAGAATGGGATTAGCCGACCAAGTGTATGTTGTA
ACATCTTCAGACTACATGGCATTATATGCTGCAAATAATATATGTAATGGAATGAGTCAATTTGCCGTTCGTGGCGGGAG
CAATTTAGGCGGCATTATTTATAACGTTAGGGGTTCAATGGACGCCTTTGATATCGTTGATGAATTTGCAAAACATTTAA
ATGGTAAAATAATTGGAAAGGTTCCAAATTCGTCAATAATAAATGAAGCAGAAATTGATGGAAAAACTGCGATTGAATAT
TCGCCTGATTCAGAAATAAGTAATATTTACATAGAACTTTCAAAAAAAATTTACGAAAACAATTCTGGAACAGTTCCAAA
ACCACTTGAAAATATTCAAATAATGCAGATTGGAAAAATGATTAAGGAAAGGCTTAAAAAAGAGAGGATTTTAGAATAA

Upstream 100 bases:

>100_bases
GTATGATATGTATCTTACTTTTAATTAACAGACTAAATGGAATTTTTAAGCGAATTGCACAACTTTACAATAGATTGATT
GATTATTTCCTGGTGATATA

Downstream 100 bases:

>100_bases
AAGGTGATTGTATGGATATAATTATAATTGGTGGGGGAACGTCTGGACTTCTATCCGCCCTAGTAATGGAAAAAGAAGGG
CATAATGTAACAATATTTGA

Product: nitrogenase reductase-like protein

Products: NA

Alternate protein names: Nitrogenase Fe protein 2; Nitrogenase component II; Nitrogenase reductase [H]

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MKQIAFYGKGGIGKSTTVCNLAAALSKSGKKVIVVGCDPKHDCTSNLRGGIEIPTVLDILREKGIDTLGIDTIIHENLLK
KEDILYKGFNGIYCVEAGGPKPGYGCAGRGVIVVIDLLKKMNVFKDLGVDIVLYDVLGDVVCGGFAMPLRMGLADQVYVV
TSSDYMALYAANNICNGMSQFAVRGGSNLGGIIYNVRGSMDAFDIVDEFAKHLNGKIIGKVPNSSIINEAEIDGKTAIEY
SPDSEISNIYIELSKKIYENNSGTVPKPLENIQIMQIGKMIKERLKKERILE

Sequences:

>Translated_292_residues
MKQIAFYGKGGIGKSTTVCNLAAALSKSGKKVIVVGCDPKHDCTSNLRGGIEIPTVLDILREKGIDTLGIDTIIHENLLK
KEDILYKGFNGIYCVEAGGPKPGYGCAGRGVIVVIDLLKKMNVFKDLGVDIVLYDVLGDVVCGGFAMPLRMGLADQVYVV
TSSDYMALYAANNICNGMSQFAVRGGSNLGGIIYNVRGSMDAFDIVDEFAKHLNGKIIGKVPNSSIINEAEIDGKTAIEY
SPDSEISNIYIELSKKIYENNSGTVPKPLENIQIMQIGKMIKERLKKERILE
>Mature_292_residues
MKQIAFYGKGGIGKSTTVCNLAAALSKSGKKVIVVGCDPKHDCTSNLRGGIEIPTVLDILREKGIDTLGIDTIIHENLLK
KEDILYKGFNGIYCVEAGGPKPGYGCAGRGVIVVIDLLKKMNVFKDLGVDIVLYDVLGDVVCGGFAMPLRMGLADQVYVV
TSSDYMALYAANNICNGMSQFAVRGGSNLGGIIYNVRGSMDAFDIVDEFAKHLNGKIIGKVPNSSIINEAEIDGKTAIEY
SPDSEISNIYIELSKKIYENNSGTVPKPLENIQIMQIGKMIKERLKKERILE

Specific function: The key enzymatic reactions in nitrogen fixation are catalyzed by the nitrogenase complex, which has 2 components:the iron protein and the molybdenum-iron protein [H]

COG id: COG1348

COG function: function code P; Nitrogenase subunit NifH (ATPase)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NifH/BchL/ChlL family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000392
- InterPro:   IPR005977 [H]

Pfam domain/function: PF00142 Fer4_NifH [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 31605; Mature: 31605

Theoretical pI: Translated: 7.27; Mature: 7.27

Prosite motif: PS00746 NIFH_FRXC_1 ; PS00692 NIFH_FRXC_2 ; PS51026 NIFH_FRXC_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQIAFYGKGGIGKSTTVCNLAAALSKSGKKVIVVGCDPKHDCTSNLRGGIEIPTVLDIL
CCCEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHCCCCCCHHHHHHHH
REKGIDTLGIDTIIHENLLKKEDILYKGFNGIYCVEAGGPKPGYGCAGRGVIVVIDLLKK
HHCCCCCCCHHHHHHHHHHHHHHHHEECCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHH
MNVFKDLGVDIVLYDVLGDVVCGGFAMPLRMGLADQVYVVTSSDYMALYAANNICNGMSQ
HHHHHHCCCCEEHHHHHHHHHHCCHHHHHHCCCCCEEEEEECCCEEEEEECCHHHHHHHH
FAVRGGSNLGGIIYNVRGSMDAFDIVDEFAKHLNGKIIGKVPNSSIINEAEIDGKTAIEY
HHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCEEEEECCCCCCCCHHCCCCCEEEEE
SPDSEISNIYIELSKKIYENNSGTVPKPLENIQIMQIGKMIKERLKKERILE
CCCCCHHHHHHEEHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKQIAFYGKGGIGKSTTVCNLAAALSKSGKKVIVVGCDPKHDCTSNLRGGIEIPTVLDIL
CCCEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHCCCCCCHHHHHHHH
REKGIDTLGIDTIIHENLLKKEDILYKGFNGIYCVEAGGPKPGYGCAGRGVIVVIDLLKK
HHCCCCCCCHHHHHHHHHHHHHHHHEECCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHH
MNVFKDLGVDIVLYDVLGDVVCGGFAMPLRMGLADQVYVVTSSDYMALYAANNICNGMSQ
HHHHHHCCCCEEHHHHHHHHHHCCHHHHHHCCCCCEEEEEECCCEEEEEECCHHHHHHHH
FAVRGGSNLGGIIYNVRGSMDAFDIVDEFAKHLNGKIIGKVPNSSIINEAEIDGKTAIEY
HHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCEEEEECCCCCCCCHHCCCCCEEEEE
SPDSEISNIYIELSKKIYENNSGTVPKPLENIQIMQIGKMIKERLKKERILE
CCCCCHHHHHHEEHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2838639 [H]