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The map label for this gene is ilvG [C]

Identifier: 150399891

GI number: 150399891

Start: 1161148

End: 1162653

Strand: Reverse

Name: ilvG [C]

Synonym: Mevan_1147

Alternate gene names: 150399891

Gene position: 1162653-1161148 (Counterclockwise)

Preceding gene: 150399892

Following gene: 150399890

Centisome position: 67.59

GC content: 30.74

Gene sequence:

>1506_bases
ATGAAATATGTCGAATCCATAATTGATTTTTTTGAAAATAATCATGTAAAAACGATTTTTTCATATCCTGGCGAACAAAT
ACTGCCACTTTATAGGAAATTACATGAAAATAGTAATATAAAATGTATTGACGTAAAACATGAGCAGTCTTCAGCCCATG
CTGCTGATGGATATTTTAGAATAACCAATAAAACAGGAATATGTCTTTCAACTGCAGGCCCTGGTGCAACTAACCTAACA
ACTGGAATTGCAACTGCTTTTAAAGACTCTTCGTCAATAATTGCAATTACTGGGCGTTGTGATAAAAAGTATATTGGTAA
AAACTATTTTCAAGAACTCCCAATGGAATTTTTAAATTTTAGTTCAGGATACTTCACTAAAGTTGCGGATTTATGTTATT
TTAAAGAAGCTTTTACAAATTCAAAAAATCTTAAAAAACCAATTCAAATAAACATTCCTTCTAACATGTACTATGAAAAT
ACAATAACTTCCAAAATGAATCTTGATGAATGTTTTGACAATTGCAAAATAGAAACTGCATTAAATGATATTAAAAAACA
TGAACATTCGAAAAACCCTGTTTTACTAATTGGCCAAGGAATATATGGAAATTTATCGTATAATGAAATAGGGGATCTAA
ATGATATTTTAAAAAATCTTGAAATGCCCATTGTAACGACTTATCCTGCAAGAGGGGTTGTTGATGAAAGTTTAGAAAAT
TGTTTTGGACTTATAGGTAGGAGGGGAAACAGTGTATCAAATTGCGCCCTTTTAAGTGCAGATACTATATTTTCAATTGG
TTCAAGCTTTTCTTACAATACACTTTTTGAAAGTATCAGGGATAAAATTTTATATAAGGTCGTTCCCCTTAATTTTGGTA
TCAATAGTACCAGTAATATTTCAACTATTGTAAACAGTTTCAATGAAACCTTTTCAAAGGCCCTTAAAACTGATTTTCCA
GACTTACGCCATAATAAGTTCCAATTTGGCGATTATTCAAATAAAATAAATGAAATACTGGTAAATTTACCAGATAATGC
GATAATAACGACAGATGCAGGAAACCATACTGTTTTTGTTTCACTATTAAAAAAATGCGTGGCTCCAAAAAACATTATAT
CGTCTCATTCAATGGGTACTATGGGTTTTGGACTTTCAGCATCTATTGGTGTCAAATTTGGATGTCTTGATTATAATATA
AATCGTGAAGTCATATCCATTAGTGGAGATGGCGGTTTTTCAATGAATATTCAAGAACTTGGAACCGTTTTTGAAAACAA
TTTAAAAATACTCATTATAGTAATGAAGAACAATCATTTAAACATGTTTGGCAGAATTAAAAACCCAGATTTCAACAAAA
TTGCCGATGCATACGGGATAGACAGTGTATACATCCAAAATGCAGACGAAATTTGTGAAAATGTGCATTACTTTTTGAAA
AATAACAAACCTTACGTCATGGTCGTAGAATGCGAAAATGAAAAACTTCCAAAGCCGTTTATTTAA

Upstream 100 bases:

>100_bases
TATATAGAAAATATAAAAGATACTATGGTTAAAAATAATATTGAGTTTGAAGAAATAACTTATTAAATTATTAACTCATT
AAATTATTGAAAGTGAATTT

Downstream 100 bases:

>100_bases
AAATTATATTTATTTCGGTGAAAATATGTCAAACGAATTATTTGAAACCCTTACGAGCAATATCCATTTTAACGTGACAG
AATTAGGTAGAAAAACGTGG

Product: thiamine pyrophosphate binding domain-containing protein

Products: 2-acetolactate; CO2

Alternate protein names: NA

Number of amino acids: Translated: 501; Mature: 501

Protein sequence:

>501_residues
MKYVESIIDFFENNHVKTIFSYPGEQILPLYRKLHENSNIKCIDVKHEQSSAHAADGYFRITNKTGICLSTAGPGATNLT
TGIATAFKDSSSIIAITGRCDKKYIGKNYFQELPMEFLNFSSGYFTKVADLCYFKEAFTNSKNLKKPIQINIPSNMYYEN
TITSKMNLDECFDNCKIETALNDIKKHEHSKNPVLLIGQGIYGNLSYNEIGDLNDILKNLEMPIVTTYPARGVVDESLEN
CFGLIGRRGNSVSNCALLSADTIFSIGSSFSYNTLFESIRDKILYKVVPLNFGINSTSNISTIVNSFNETFSKALKTDFP
DLRHNKFQFGDYSNKINEILVNLPDNAIITTDAGNHTVFVSLLKKCVAPKNIISSHSMGTMGFGLSASIGVKFGCLDYNI
NREVISISGDGGFSMNIQELGTVFENNLKILIIVMKNNHLNMFGRIKNPDFNKIADAYGIDSVYIQNADEICENVHYFLK
NNKPYVMVVECENEKLPKPFI

Sequences:

>Translated_501_residues
MKYVESIIDFFENNHVKTIFSYPGEQILPLYRKLHENSNIKCIDVKHEQSSAHAADGYFRITNKTGICLSTAGPGATNLT
TGIATAFKDSSSIIAITGRCDKKYIGKNYFQELPMEFLNFSSGYFTKVADLCYFKEAFTNSKNLKKPIQINIPSNMYYEN
TITSKMNLDECFDNCKIETALNDIKKHEHSKNPVLLIGQGIYGNLSYNEIGDLNDILKNLEMPIVTTYPARGVVDESLEN
CFGLIGRRGNSVSNCALLSADTIFSIGSSFSYNTLFESIRDKILYKVVPLNFGINSTSNISTIVNSFNETFSKALKTDFP
DLRHNKFQFGDYSNKINEILVNLPDNAIITTDAGNHTVFVSLLKKCVAPKNIISSHSMGTMGFGLSASIGVKFGCLDYNI
NREVISISGDGGFSMNIQELGTVFENNLKILIIVMKNNHLNMFGRIKNPDFNKIADAYGIDSVYIQNADEICENVHYFLK
NNKPYVMVVECENEKLPKPFI
>Mature_501_residues
MKYVESIIDFFENNHVKTIFSYPGEQILPLYRKLHENSNIKCIDVKHEQSSAHAADGYFRITNKTGICLSTAGPGATNLT
TGIATAFKDSSSIIAITGRCDKKYIGKNYFQELPMEFLNFSSGYFTKVADLCYFKEAFTNSKNLKKPIQINIPSNMYYEN
TITSKMNLDECFDNCKIETALNDIKKHEHSKNPVLLIGQGIYGNLSYNEIGDLNDILKNLEMPIVTTYPARGVVDESLEN
CFGLIGRRGNSVSNCALLSADTIFSIGSSFSYNTLFESIRDKILYKVVPLNFGINSTSNISTIVNSFNETFSKALKTDFP
DLRHNKFQFGDYSNKINEILVNLPDNAIITTDAGNHTVFVSLLKKCVAPKNIISSHSMGTMGFGLSASIGVKFGCLDYNI
NREVISISGDGGFSMNIQELGTVFENNLKILIIVMKNNHLNMFGRIKNPDFNKIADAYGIDSVYIQNADEICENVHYFLK
NNKPYVMVVECENEKLPKPFI

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081685, Length=534, Percent_Identity=27.3408239700375, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI1790104, Length=561, Percent_Identity=24.9554367201426, Blast_Score=183, Evalue=3e-47,
Organism=Escherichia coli, GI1786717, Length=492, Percent_Identity=21.1382113821138, Blast_Score=105, Evalue=9e-24,
Organism=Escherichia coli, GI1787096, Length=540, Percent_Identity=21.8518518518519, Blast_Score=101, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6323755, Length=559, Percent_Identity=24.6869409660107, Blast_Score=180, Evalue=4e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF02776 TPP_enzyme_N [H]

EC number: 2.2.1.6

Molecular weight: Translated: 56083; Mature: 56083

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: PS00639 THIOL_PROTEASE_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYVESIIDFFENNHVKTIFSYPGEQILPLYRKLHENSNIKCIDVKHEQSSAHAADGYFR
CCHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCEEE
ITNKTGICLSTAGPGATNLTTGIATAFKDSSSIIAITGRCDKKYIGKNYFQELPMEFLNF
EECCCCEEEECCCCCCCCHHHHHHHHCCCCCCEEEEECCCCHHHHCHHHHHHCCHHHHCC
SSGYFTKVADLCYFKEAFTNSKNLKKPIQINIPSNMYYENTITSKMNLDECFDNCKIETA
CCCHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCEEECCCCCCCCCHHHHHHCCEEHHH
LNDIKKHEHSKNPVLLIGQGIYGNLSYNEIGDLNDILKNLEMPIVTTYPARGVVDESLEN
HHHHHHHHCCCCCEEEEECCCCCCCCHHHCCCHHHHHHHCCCCEEEECCCCCCHHHHHHH
CFGLIGRRGNSVSNCALLSADTIFSIGSSFSYNTLFESIRDKILYKVVPLNFGINSTSNI
HHHHHCCCCCCCCCEEEEECHHHHHCCCCCCHHHHHHHHHHHHEEEEEEEECCCCCCCHH
STIVNSFNETFSKALKTDFPDLRHNKFQFGDYSNKINEILVNLPDNAIITTDAGNHTVFV
HHHHHHHHHHHHHHHHCCCCCCCCCCEECCCHHCCHHHEEEECCCCEEEEECCCCCCHHH
SLLKKCVAPKNIISSHSMGTMGFGLSASIGVKFGCLDYNINREVISISGDGGFSMNIQEL
HHHHHHCCCHHHHHCCCCCCCCCCCEEECCEEEEEEEECCCCEEEEEECCCCCEEEHHHH
GTVFENNLKILIIVMKNNHLNMFGRIKNPDFNKIADAYGIDSVYIQNADEICENVHYFLK
HHHHHCCCEEEEEEEECCCEEEEEEECCCCHHHHHHHHCCCEEEECCHHHHHHHHHHHHC
NNKPYVMVVECENEKLPKPFI
CCCCEEEEEEECCCCCCCCCC
>Mature Secondary Structure
MKYVESIIDFFENNHVKTIFSYPGEQILPLYRKLHENSNIKCIDVKHEQSSAHAADGYFR
CCHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCEEE
ITNKTGICLSTAGPGATNLTTGIATAFKDSSSIIAITGRCDKKYIGKNYFQELPMEFLNF
EECCCCEEEECCCCCCCCHHHHHHHHCCCCCCEEEEECCCCHHHHCHHHHHHCCHHHHCC
SSGYFTKVADLCYFKEAFTNSKNLKKPIQINIPSNMYYENTITSKMNLDECFDNCKIETA
CCCHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCEEECCCCCCCCCHHHHHHCCEEHHH
LNDIKKHEHSKNPVLLIGQGIYGNLSYNEIGDLNDILKNLEMPIVTTYPARGVVDESLEN
HHHHHHHHCCCCCEEEEECCCCCCCCHHHCCCHHHHHHHCCCCEEEECCCCCCHHHHHHH
CFGLIGRRGNSVSNCALLSADTIFSIGSSFSYNTLFESIRDKILYKVVPLNFGINSTSNI
HHHHHCCCCCCCCCEEEEECHHHHHCCCCCCHHHHHHHHHHHHEEEEEEEECCCCCCCHH
STIVNSFNETFSKALKTDFPDLRHNKFQFGDYSNKINEILVNLPDNAIITTDAGNHTVFV
HHHHHHHHHHHHHHHHCCCCCCCCCCEECCCHHCCHHHEEEECCCCEEEEECCCCCCHHH
SLLKKCVAPKNIISSHSMGTMGFGLSASIGVKFGCLDYNINREVISISGDGGFSMNIQEL
HHHHHHCCCHHHHHCCCCCCCCCCCEEECCEEEEEEEECCCCEEEEEECCCCCEEEHHHH
GTVFENNLKILIIVMKNNHLNMFGRIKNPDFNKIADAYGIDSVYIQNADEICENVHYFLK
HHHHHCCCEEEEEEEECCCEEEEEEECCCCHHHHHHHHCCCEEEECCHHHHHHHHHHHHC
NNKPYVMVVECENEKLPKPFI
CCCCEEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 4002 [C]

Specific activity: 53

Km value (mM): 5 {2-oxobutanoate}} 1 {2-oxobutanoate}} 7.6 {pyruvate}} 7 {pyruvate}} 2.9 {pyruvate}} 2.6 {pyruvate}} 1.3 {pyruvate}} 1 {pyruvate}} [C]

Substrates: pyruvate

Specific reaction: 2 pyruvate = 2-acetolactate + CO2

General reaction: C-C bond formation; decarboxylation [C]

Inhibitor: @OXOB02.txt*2-oxobutanoate!; chlorimuronethyl; chlorsulphuron; Ile; imazapyr; Leu; Val [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]