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The map label for this gene is hyfA [C]

Identifier: 150399563

GI number: 150399563

Start: 843510

End: 844010

Strand: Reverse

Name: hyfA [C]

Synonym: Mevan_0812

Alternate gene names: 150399563

Gene position: 844010-843510 (Counterclockwise)

Preceding gene: 150399564

Following gene: 150399562

Centisome position: 49.07

GC content: 38.92

Gene sequence:

>501_bases
ATGAAAAAAGTTATGATGGTTAACGATACTTGCGATAACTGTGGGGATTGCGTTAAGGCATGCTCTGAAATTCACGAAAT
AAGTGGCATTAGTATTTGGGAGCATAATGGTAGGTACCTACAGGTAGTTTGCCAGCACTGTACCTCTTCCCCCTGTAGCG
AAGTATGCCCTGTTTCTGCAATTGAATCAAAAGATGGCGTAATTTATCTAGATAAAGAGACATGCATTGGATGCGGGCTC
TGTGCAATGGCATGTCCGTTTGGTGCAATTTACGTTACTGGAAAAACTGCCCACAAGTGCGACTTATGTTACAATCGAGA
AAATCATGCATGTGTTAAAGCATGCAGTAAAAGATGCCTTGACGTCTTAAATGTTGATGAAATTGTAATGGACAAAAAAC
TTAAAAATATTGAAAATTTAGCCGTAGTTGGGTCGAAATCAAAACCTAAAAAGAAAGGACTTCTCTCTTTAATTACTGCA
TCTTCACGATGCAACCCTTAA

Upstream 100 bases:

>100_bases
GATACCGGCACCTGACTCCTGCAGACATTTCCAAAATACAAGAAGCAATTGATTTGAAATGTCAGGAATACGGTCTTTAA
TTAATTTTTAAGGTGCAATC

Downstream 100 bases:

>100_bases
TTTAATCTGGTGAAGTTATGAAAGTAATACCAAATATCGGGCTTTGTGTAAGCTGTGGAAAATGCGAAAGAATATGCCCT
TTAAATGCAATCCATGTTTT

Product: 4Fe-4S ferredoxin iron-sulfur binding domain-containing protein

Products: H2; oxidized ferredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 166; Mature: 166

Protein sequence:

>166_residues
MKKVMMVNDTCDNCGDCVKACSEIHEISGISIWEHNGRYLQVVCQHCTSSPCSEVCPVSAIESKDGVIYLDKETCIGCGL
CAMACPFGAIYVTGKTAHKCDLCYNRENHACVKACSKRCLDVLNVDEIVMDKKLKNIENLAVVGSKSKPKKKGLLSLITA
SSRCNP

Sequences:

>Translated_166_residues
MKKVMMVNDTCDNCGDCVKACSEIHEISGISIWEHNGRYLQVVCQHCTSSPCSEVCPVSAIESKDGVIYLDKETCIGCGL
CAMACPFGAIYVTGKTAHKCDLCYNRENHACVKACSKRCLDVLNVDEIVMDKKLKNIENLAVVGSKSKPKKKGLLSLITA
SSRCNP
>Mature_166_residues
MKKVMMVNDTCDNCGDCVKACSEIHEISGISIWEHNGRYLQVVCQHCTSSPCSEVCPVSAIESKDGVIYLDKETCIGCGL
CAMACPFGAIYVTGKTAHKCDLCYNRENHACVKACSKRCLDVLNVDEIVMDKKLKNIENLAVVGSKSKPKKKGLLSLITA
SSRCNP

Specific function: Probable Electron Transfer Protein For Hydrogenase 4. [C]

COG id: COG1142

COG function: function code C; Fe-S-cluster-containing hydrogenase components 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI87082114, Length=180, Percent_Identity=32.2222222222222, Blast_Score=87, Evalue=8e-19,
Organism=Escherichia coli, GI1788811, Length=145, Percent_Identity=35.8620689655172, Blast_Score=86, Evalue=1e-18,
Organism=Escherichia coli, GI87082180, Length=99, Percent_Identity=38.3838383838384, Blast_Score=83, Evalue=9e-18,
Organism=Escherichia coli, GI2367245, Length=147, Percent_Identity=34.6938775510204, Blast_Score=81, Evalue=3e-17,
Organism=Escherichia coli, GI1787122, Length=121, Percent_Identity=37.1900826446281, Blast_Score=79, Evalue=1e-16,
Organism=Escherichia coli, GI1787872, Length=121, Percent_Identity=35.5371900826446, Blast_Score=77, Evalue=6e-16,
Organism=Escherichia coli, GI1789079, Length=180, Percent_Identity=28.3333333333333, Blast_Score=75, Evalue=1e-15,
Organism=Escherichia coli, GI87082179, Length=167, Percent_Identity=29.3413173652695, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI2367345, Length=146, Percent_Identity=32.1917808219178, Blast_Score=70, Evalue=8e-14,
Organism=Escherichia coli, GI1789067, Length=134, Percent_Identity=32.089552238806, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787740, Length=86, Percent_Identity=34.8837209302326, Blast_Score=65, Evalue=2e-12,
Organism=Escherichia coli, GI1787478, Length=86, Percent_Identity=33.7209302325581, Blast_Score=64, Evalue=3e-12,
Organism=Escherichia coli, GI226510944, Length=119, Percent_Identity=31.9327731092437, Blast_Score=63, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 18056; Mature: 18056

Theoretical pI: Translated: 7.81; Mature: 7.81

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

10.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
13.9 %Cys+Met (Translated Protein)
10.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
13.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVMMVNDTCDNCGDCVKACSEIHEISGISIWEHNGRYLQVVCQHCTSSPCSEVCPVSA
CCCEEEECCCCCCHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHCCCCCHHCCCCCC
IESKDGVIYLDKETCIGCGLCAMACPFGAIYVTGKTAHKCDLCYNRENHACVKACSKRCL
CCCCCCEEEEECCCCHHHHHHHHHCCCCEEEEECCCCCCCCEEECCCCCHHHHHHHHHHH
DVLNVDEIVMDKKLKNIENLAVVGSKSKPKKKGLLSLITASSRCNP
HHCCHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKKVMMVNDTCDNCGDCVKACSEIHEISGISIWEHNGRYLQVVCQHCTSSPCSEVCPVSA
CCCEEEECCCCCCHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHCCCCCHHCCCCCC
IESKDGVIYLDKETCIGCGLCAMACPFGAIYVTGKTAHKCDLCYNRENHACVKACSKRCL
CCCCCCEEEEECCCCHHHHHHHHHCCCCEEEEECCCCCCCCEEECCCCCHHHHHHHHHHH
DVLNVDEIVMDKKLKNIENLAVVGSKSKPKKKGLLSLITASSRCNP
HHCCHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]