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The map label for this gene is purA

Identifier: 150399494

GI number: 150399494

Start: 785606

End: 786622

Strand: Reverse

Name: purA

Synonym: Mevan_0743

Alternate gene names: 150399494

Gene position: 786622-785606 (Counterclockwise)

Preceding gene: 150399495

Following gene: 150399493

Centisome position: 45.73

GC content: 38.74

Gene sequence:

>1017_bases
ATGACATGTACCATAATTGTTGGTGGCCAATGGGGCGACGAAGGTAAAGGAAAAGTTATAAGCTACCTTTGTAAAAAAGA
CAACCCTTCAATTATTGCAAGGGGGGGCGTTGGCCCTAATGCAGGACATACTGTTGAAGTAGACGGAGAAAAATATGGAA
TTAGAATGGTTCCAACCGGTTTTCCAAATGTTAACGCAAAACTTGCAGTTGGTGCAGGAGTTTTGACTGACCCTGAAGTA
CTTGTTAGGGAAATTGAAAAACTTCAAAAGTTTAACGTTGGAGAAAGAATAATTATAGACTACCGGTGTGGAGTAATTGA
ATCAAAGCACAGGGATCTTGATAAATCAAATGAACACCTTTCAAAAGAAATTGGGTCAACTGGAACCGGATGCGGCCCCG
CAAATGTTGATAGGGCAATGAGGACGTTAAAATTAGCAAAAGATGTTTCAGAGCTTTCTAAATATTTGGGGGACGTTTCA
GAAGCAGTAAATAATGCAATTGAATCCGGAGATAATGTAATTATTGAAGGGACACAAGGTTCACTTCTTTCACTTTACTA
CGGAAGTTATCCTTACGTTACATCTAAAGACACGAATGCAGCATCATTTGCAGCAGATGTTGGTTTAGGCCCTACAAAAA
TTGATGAAGTAGTAGCTGTTTTCAAATCATACCCAACAAGGGTTGGTGAAGGGCCATTTCCAACAGAAATGTCATTAGAA
GAAGCCGAAAAATTAGGTGTAGTTGAATATGGTACAGTTACAGGCAGGAGAAGACGGGTTGGATACTTTGACTTTGAACT
TGCTAAAAAAGTTTGTAAGCTAAATGGTGCAACTCAAATTGCAATAACGTGCCTTGACAAATACGACCCACTATGCTATG
GTATAATTGACTATAATGAACTTTCCGAAAAAGGAAAGGCATTCATTAAAGAAGTAGAAGAGAAAGTTGGGGTTAGGGTA
ACCATTATATCAACAGGGCCCGAACTTAGCCAGACGATTGATATTAGAAATAAATAA

Upstream 100 bases:

>100_bases
ATTAATTTTCTTTTTTTAGGAATTCACATCTATTTATTCAGATATTAGTAGGGTTAAACTCCTCCGAATAGTTTTGAGGA
TTTAAATGGAGGTTCTGTAA

Downstream 100 bases:

>100_bases
TAAAAATACATAACTATAATAATTTTAAATGATTACTTTTTTATGATTATAATTATTATCACGTTATTCTACGTTTTTAG
ATTGGTAGGGATACGATGAC

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 338; Mature: 337

Protein sequence:

>338_residues
MTCTIIVGGQWGDEGKGKVISYLCKKDNPSIIARGGVGPNAGHTVEVDGEKYGIRMVPTGFPNVNAKLAVGAGVLTDPEV
LVREIEKLQKFNVGERIIIDYRCGVIESKHRDLDKSNEHLSKEIGSTGTGCGPANVDRAMRTLKLAKDVSELSKYLGDVS
EAVNNAIESGDNVIIEGTQGSLLSLYYGSYPYVTSKDTNAASFAADVGLGPTKIDEVVAVFKSYPTRVGEGPFPTEMSLE
EAEKLGVVEYGTVTGRRRRVGYFDFELAKKVCKLNGATQIAITCLDKYDPLCYGIIDYNELSEKGKAFIKEVEEKVGVRV
TIISTGPELSQTIDIRNK

Sequences:

>Translated_338_residues
MTCTIIVGGQWGDEGKGKVISYLCKKDNPSIIARGGVGPNAGHTVEVDGEKYGIRMVPTGFPNVNAKLAVGAGVLTDPEV
LVREIEKLQKFNVGERIIIDYRCGVIESKHRDLDKSNEHLSKEIGSTGTGCGPANVDRAMRTLKLAKDVSELSKYLGDVS
EAVNNAIESGDNVIIEGTQGSLLSLYYGSYPYVTSKDTNAASFAADVGLGPTKIDEVVAVFKSYPTRVGEGPFPTEMSLE
EAEKLGVVEYGTVTGRRRRVGYFDFELAKKVCKLNGATQIAITCLDKYDPLCYGIIDYNELSEKGKAFIKEVEEKVGVRV
TIISTGPELSQTIDIRNK
>Mature_337_residues
TCTIIVGGQWGDEGKGKVISYLCKKDNPSIIARGGVGPNAGHTVEVDGEKYGIRMVPTGFPNVNAKLAVGAGVLTDPEVL
VREIEKLQKFNVGERIIIDYRCGVIESKHRDLDKSNEHLSKEIGSTGTGCGPANVDRAMRTLKLAKDVSELSKYLGDVSE
AVNNAIESGDNVIIEGTQGSLLSLYYGSYPYVTSKDTNAASFAADVGLGPTKIDEVVAVFKSYPTRVGEGPFPTEMSLEE
AEKLGVVEYGTVTGRRRRVGYFDFELAKKVCKLNGATQIAITCLDKYDPLCYGIIDYNELSEKGKAFIKEVEEKVGVRVT
IISTGPELSQTIDIRNK

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI22748717, Length=339, Percent_Identity=32.4483775811209, Blast_Score=152, Evalue=3e-37,
Organism=Homo sapiens, GI34577063, Length=339, Percent_Identity=31.2684365781711, Blast_Score=151, Evalue=9e-37,
Organism=Homo sapiens, GI40316944, Length=305, Percent_Identity=30.8196721311475, Blast_Score=123, Evalue=2e-28,
Organism=Escherichia coli, GI1790620, Length=425, Percent_Identity=32, Blast_Score=201, Evalue=8e-53,
Organism=Caenorhabditis elegans, GI25146553, Length=340, Percent_Identity=31.1764705882353, Blast_Score=171, Evalue=5e-43,
Organism=Caenorhabditis elegans, GI25146555, Length=340, Percent_Identity=31.1764705882353, Blast_Score=171, Evalue=5e-43,
Organism=Saccharomyces cerevisiae, GI6324109, Length=423, Percent_Identity=31.4420803782506, Blast_Score=182, Evalue=9e-47,
Organism=Drosophila melanogaster, GI21356033, Length=336, Percent_Identity=31.547619047619, Blast_Score=152, Evalue=3e-37,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_METVS (A6UQ77)

Other databases:

- EMBL:   CP000742
- RefSeq:   YP_001323261.1
- ProteinModelPortal:   A6UQ77
- SMR:   A6UQ77
- STRING:   A6UQ77
- GeneID:   5325537
- GenomeReviews:   CP000742_GR
- KEGG:   mvn:Mevan_0743
- eggNOG:   arNOG04146
- HOGENOM:   HBG658237
- OMA:   YVLGIIK
- ProtClustDB:   PRK04293
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 36588; Mature: 36457

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 13-13 ACT_SITE 43-43 BINDING 127-127 BINDING 141-141 BINDING 179-179 BINDING 194-194 BINDING 256-256 BINDING 258-258

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTCTIIVGGQWGDEGKGKVISYLCKKDNPSIIARGGVGPNAGHTVEVDGEKYGIRMVPTG
CEEEEEECCCCCCCCCCHHHHEEECCCCCCEEEECCCCCCCCCEEEECCCEECEEECCCC
FPNVNAKLAVGAGVLTDPEVLVREIEKLQKFNVGERIIIDYRCGVIESKHRDLDKSNEHL
CCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHCCCCCHHHHH
SKEIGSTGTGCGPANVDRAMRTLKLAKDVSELSKYLGDVSEAVNNAIESGDNVIIEGTQG
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCC
SLLSLYYGSYPYVTSKDTNAASFAADVGLGPTKIDEVVAVFKSYPTRVGEGPFPTEMSLE
CEEEEEECCCCEEECCCCCCHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHH
EAEKLGVVEYGTVTGRRRRVGYFDFELAKKVCKLNGATQIAITCLDKYDPLCYGIIDYNE
HHHHCCCEEECCCCCCCEECCCCCHHHHHHHHHCCCCCEEEEEEECCCCCEEEEEECHHH
LSEKGKAFIKEVEEKVGVRVTIISTGPELSQTIDIRNK
HHHHHHHHHHHHHHHCCEEEEEEECCCCCHHEEECCCC
>Mature Secondary Structure 
TCTIIVGGQWGDEGKGKVISYLCKKDNPSIIARGGVGPNAGHTVEVDGEKYGIRMVPTG
EEEEEECCCCCCCCCCHHHHEEECCCCCCEEEECCCCCCCCCEEEECCCEECEEECCCC
FPNVNAKLAVGAGVLTDPEVLVREIEKLQKFNVGERIIIDYRCGVIESKHRDLDKSNEHL
CCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHCCCCCHHHHH
SKEIGSTGTGCGPANVDRAMRTLKLAKDVSELSKYLGDVSEAVNNAIESGDNVIIEGTQG
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCC
SLLSLYYGSYPYVTSKDTNAASFAADVGLGPTKIDEVVAVFKSYPTRVGEGPFPTEMSLE
CEEEEEECCCCEEECCCCCCHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHH
EAEKLGVVEYGTVTGRRRRVGYFDFELAKKVCKLNGATQIAITCLDKYDPLCYGIIDYNE
HHHHCCCEEECCCCCCCEECCCCCHHHHHHHHHCCCCCEEEEEEECCCCCEEEEEECHHH
LSEKGKAFIKEVEEKVGVRVTIISTGPELSQTIDIRNK
HHHHHHHHHHHHHHHCCEEEEEEECCCCCHHEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA