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The map label for this gene is hyfG [C]

Identifier: 150399225

GI number: 150399225

Start: 524770

End: 525897

Strand: Reverse

Name: hyfG [C]

Synonym: Mevan_0473

Alternate gene names: 150399225

Gene position: 525897-524770 (Counterclockwise)

Preceding gene: 150399252

Following gene: 150399224

Centisome position: 30.57

GC content: 37.68

Gene sequence:

>1128_bases
ATGTACGAAGGAGAAATCGCAATTGGGCCTGTTCACGCAACAATGCTTGAACCGCATCGTTTAAGACTTTTTATTGAAGA
TGAAATAGTAAAAGATGCTGAACTTACAATTGGTGTAAACCATAGGGGTGTAGAGAGGTTAATGGAGGGACTTCCCGTTG
AAAAAGCATGTATATTGTGCGAAAAAATATGCGGAATTTGTTCACACATTCACCTTTGGAGTGCTGCAAGGGTTGTTGAA
ATCGGGTGCAAAATTGAAGTGCCAGAACGTGCAAACCATATACGGCTTATTGTAGAAGAATTAGAGAGGCTACATTCCCA
CACACTACTTTTTGGACATGCTTTTGAAATTTTGGGTCATGAAACAATGTCAATGAGAAGTTTCATGTTAAGGGAGCCCA
TAATGCAGTTATTTTTTGAAATATCCGGAAACCGAGTTCATTATTCATGCCCCATTATTGGGGGTGTACGGCCAAGATGC
AATATTTCGGATAGCCAAATTCCAGTTATACTTGATCGAATTGAAAAATATGAAGAAGGACTTACTAAATTGATAAACCG
GACATTAAATGACCCGATGATTGTTTCAAGGGTTAAAAATATTGGTATTTTGGATAGAAAAACCGCTGCAAAATTTCATG
CAGTGGGCCCGACCGCAAGGGGGAGTAACATAAAATCAGACGTAAGAAAAATGGGCTATGTTCCTGAATACGACCCTTAT
GAATTTGATGAAATATTGCTTGATGGCGGAGATGTACTTTCAAGAGTTGCTGTTAGATTTTACGAGTGTTTTGAAAGTAT
AAAAATCATCAAGCAGGCAATAAATATGCTAAAAGACTGTAAAGACAATAGAATTTACAATCCAAACTATGAACTCCACA
AATTTAAGCCAATTGATTGTTATACTGAAGCACAGAGGGGGGAACAATACTACTCCTATGGGCTTGATGATGAGGGGCAA
GTAAGACAGGCAAAAGTAAGGACTCCAACTGCAACAAACCTTGGAGCAATGGAAGAAATTGTAAAAGGTTACCATGTAAG
CGATGCAGAATTAATTATCGCAAGTTGCGACCCATGTTTTACTTGTACTGATAGATTAATGGTTTTAAAAGAGCCATTTA
AAAAATAA

Upstream 100 bases:

>100_bases
AATGATGATTAATTAATAACGATAAATATTAGTTTTCTATATACTTACGTATAATTTCAATCGAGTTAGATTATTATAAA
TTACTTTAAAAGTGAGAATC

Downstream 100 bases:

>100_bases
TTTTTTTACGTTTTAAGGTGGTTTTTTGCTTATAAAAGACGTCATGAACGAAGACTTTTTAACGTTTTTACTTAATGATA
TAGCTGGAGAAGTAATTCAA

Product: NADH dehydrogenase (quinone)

Products: NAD+; ubiquinol

Alternate protein names: NA

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MYEGEIAIGPVHATMLEPHRLRLFIEDEIVKDAELTIGVNHRGVERLMEGLPVEKACILCEKICGICSHIHLWSAARVVE
IGCKIEVPERANHIRLIVEELERLHSHTLLFGHAFEILGHETMSMRSFMLREPIMQLFFEISGNRVHYSCPIIGGVRPRC
NISDSQIPVILDRIEKYEEGLTKLINRTLNDPMIVSRVKNIGILDRKTAAKFHAVGPTARGSNIKSDVRKMGYVPEYDPY
EFDEILLDGGDVLSRVAVRFYECFESIKIIKQAINMLKDCKDNRIYNPNYELHKFKPIDCYTEAQRGEQYYSYGLDDEGQ
VRQAKVRTPTATNLGAMEEIVKGYHVSDAELIIASCDPCFTCTDRLMVLKEPFKK

Sequences:

>Translated_375_residues
MYEGEIAIGPVHATMLEPHRLRLFIEDEIVKDAELTIGVNHRGVERLMEGLPVEKACILCEKICGICSHIHLWSAARVVE
IGCKIEVPERANHIRLIVEELERLHSHTLLFGHAFEILGHETMSMRSFMLREPIMQLFFEISGNRVHYSCPIIGGVRPRC
NISDSQIPVILDRIEKYEEGLTKLINRTLNDPMIVSRVKNIGILDRKTAAKFHAVGPTARGSNIKSDVRKMGYVPEYDPY
EFDEILLDGGDVLSRVAVRFYECFESIKIIKQAINMLKDCKDNRIYNPNYELHKFKPIDCYTEAQRGEQYYSYGLDDEGQ
VRQAKVRTPTATNLGAMEEIVKGYHVSDAELIIASCDPCFTCTDRLMVLKEPFKK
>Mature_375_residues
MYEGEIAIGPVHATMLEPHRLRLFIEDEIVKDAELTIGVNHRGVERLMEGLPVEKACILCEKICGICSHIHLWSAARVVE
IGCKIEVPERANHIRLIVEELERLHSHTLLFGHAFEILGHETMSMRSFMLREPIMQLFFEISGNRVHYSCPIIGGVRPRC
NISDSQIPVILDRIEKYEEGLTKLINRTLNDPMIVSRVKNIGILDRKTAAKFHAVGPTARGSNIKSDVRKMGYVPEYDPY
EFDEILLDGGDVLSRVAVRFYECFESIKIIKQAINMLKDCKDNRIYNPNYELHKFKPIDCYTEAQRGEQYYSYGLDDEGQ
VRQAKVRTPTATNLGAMEEIVKGYHVSDAELIIASCDPCFTCTDRLMVLKEPFKK

Specific function: Unknown

COG id: COG3261

COG function: function code C; Ni,Fe-hydrogenase III large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 49 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI4758786, Length=390, Percent_Identity=25.3846153846154, Blast_Score=102, Evalue=7e-22,
Organism=Homo sapiens, GI260898743, Length=380, Percent_Identity=25, Blast_Score=97, Evalue=3e-20,
Organism=Escherichia coli, GI1788832, Length=368, Percent_Identity=33.1521739130435, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI1789076, Length=377, Percent_Identity=32.8912466843501, Blast_Score=178, Evalue=6e-46,
Organism=Escherichia coli, GI145693162, Length=393, Percent_Identity=24.9363867684478, Blast_Score=98, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17568379, Length=388, Percent_Identity=26.8041237113402, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17555284, Length=385, Percent_Identity=25.7142857142857, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI221459469, Length=391, Percent_Identity=26.5984654731458, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24638644, Length=387, Percent_Identity=27.6485788113695, Blast_Score=114, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001135
- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00346 Complex1_49kDa; PF00374 NiFeSe_Hases [H]

EC number: 1.6.5.3

Molecular weight: Translated: 42844; Mature: 42844

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00507 NI_HGENASE_L_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYEGEIAIGPVHATMLEPHRLRLFIEDEIVKDAELTIGVNHRGVERLMEGLPVEKACILC
CCCCCEEECCCHHHHCCCCCEEEEEEHHHCCCCEEEEECCHHHHHHHHHCCCHHHHHHHH
EKICGICSHIHLWSAARVVEIGCKIEVPERANHIRLIVEELERLHSHTLLFGHAFEILGH
HHHHHHHHHHHHHHHHHEEEECCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
ETMSMRSFMLREPIMQLFFEISGNRVHYSCPIIGGVRPRCNISDSQIPVILDRIEKYEEG
HHHHHHHHHHHHHHHHHHHHHCCCEEEEECCEECCCCCCCCCCCCCCHHHHHHHHHHHHH
LTKLINRTLNDPMIVSRVKNIGILDRKTAAKFHAVGPTARGSNIKSDVRKMGYVPEYDPY
HHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHEEECCCCCCCCCHHHHHHHCCCCCCCCCC
EFDEILLDGGDVLSRVAVRFYECFESIKIIKQAINMLKDCKDNRIYNPNYELHKFKPIDC
CHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCEEEECCCCH
YTEAQRGEQYYSYGLDDEGQVRQAKVRTPTATNLGAMEEIVKGYHVSDAELIIASCDPCF
HHHHHCCCHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHCCCCCCCCEEEEECCCCCH
TCTDRLMVLKEPFKK
HHHHHHHHHHCCCCC
>Mature Secondary Structure
MYEGEIAIGPVHATMLEPHRLRLFIEDEIVKDAELTIGVNHRGVERLMEGLPVEKACILC
CCCCCEEECCCHHHHCCCCCEEEEEEHHHCCCCEEEEECCHHHHHHHHHCCCHHHHHHHH
EKICGICSHIHLWSAARVVEIGCKIEVPERANHIRLIVEELERLHSHTLLFGHAFEILGH
HHHHHHHHHHHHHHHHHEEEECCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
ETMSMRSFMLREPIMQLFFEISGNRVHYSCPIIGGVRPRCNISDSQIPVILDRIEKYEEG
HHHHHHHHHHHHHHHHHHHHHCCCEEEEECCEECCCCCCCCCCCCCCHHHHHHHHHHHHH
LTKLINRTLNDPMIVSRVKNIGILDRKTAAKFHAVGPTARGSNIKSDVRKMGYVPEYDPY
HHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHEEECCCCCCCCCHHHHHHHCCCCCCCCCC
EFDEILLDGGDVLSRVAVRFYECFESIKIIKQAINMLKDCKDNRIYNPNYELHKFKPIDC
CHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCEEEECCCCH
YTEAQRGEQYYSYGLDDEGQVRQAKVRTPTATNLGAMEEIVKGYHVSDAELIIASCDPCF
HHHHHCCCHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHCCCCCCCCEEEEECCCCCH
TCTDRLMVLKEPFKK
HHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]