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The map label for this gene is thrA [C]

Identifier: 150399100

GI number: 150399100

Start: 397548

End: 398945

Strand: Reverse

Name: thrA [C]

Synonym: Mevan_0346

Alternate gene names: 150399100

Gene position: 398945-397548 (Counterclockwise)

Preceding gene: 150399102

Following gene: 150399098

Centisome position: 23.19

GC content: 35.12

Gene sequence:

>1398_bases
TTGGTTACAGTAATGAAATTTGGTGGAACCTCTGTTGGTAATGGCGAAAGGATAAGAAACGTTGCAAAAATCGTTGTAAA
CAAGACAAAAGAAGATAAGGACGTATGTGTTGTAACCTCGGCAATGACACAAGTTACAAATTCACTTATCGAAATTTCTA
CACAGGCACTTGATGTTCGAGATATTGCAAAAATAAACAATTTTATCGAAGATTTAAGGATAAAACACGAAATTGCAATC
GATCAGGCAATCTTTAACCATGAAATAAAGACCGAAGTTTCAAAAACAATTGAAAGTTCGATAAATGAACTTGAAAAAGT
TCTTGTTGGTGTTTCTTACCTTGGAGAATTAACTCCTAAATCAAAAGACTTTATATTGTCCTTTGGTGAAAGACTCTCTG
CACCAATTTTAAGCGGTGCAATAAGGGATCTTGGAAAACATTCTCTTTTTTTAACAGGAAGAGATGCAGGAATTGTTACC
GATGACTCATTTAGCTGTGCAAAAGTAGTCCGACTTGAAGTTTTAGATAAGGTCTCCCCTATATTAAAAGATGGATTTAT
TCCCGTTATAACCGGATTTATCGGGGGAACTGATGATAATCAAATAACTACATTTGGAAGGGGCGGTAGTGACTATTCCG
CAGCACTAGTTGGTTCGGGATTGAATGCGGACATGGTCGAAATATGGACTGATGTTAGCGGAGTTTTGTCGGCAGACCCA
AGAATGGTCGAAAATGTAAAGCAGATTCCAAGAATGTCATATATCGAAGCAATGGAACTTGCATATTTTGGCGCTAAAGT
TCTTCACCCAAGAACAATGGAGCCAGTAATGGAAAAAAAGATTCCTTTAAGGATTAAAAATACATTTGACCCTGAAAATG
AAGGTACCTTAGTAACAGACAGTGCAGAAACATGTAATTCGGTAATAAAAGCAATTACGACAATTAAAGACGTTATATTA
ATTAATATTTTTGGTGGCGGAATGGTTGGAGTTAGCGGGACTGCTGCAAGAATATTTAACGTACTTGGAAATTCAAATGC
AAATGTAATACTAATTACACAAGGTTCATCTGAAACAAACATATCAATTGTAATATACGATGGCGAACTTGAAGCTAAAA
AATGTGTAAGAGAATTAAAAGAAGAATTTGGCGAATGCCATTTAATTAAGGATATAACCTTTGATAAAGAGGTATGTGTG
GTTTCCGTAGTCGGTTCAGGAATGAAAGGTTCAAAAGGAATTGCAGGAAAATTATTTGATGCAGTTGCAGAAAGTGGGGC
AAACATAAAAATGATTGCACAAGGTTCATCTGAAACAAACATATCGTTTGTAATTGATGAAGAAAACCTTGAAAACTGCT
TAAAAACGTTACACAAAACATTTATTGAAAATAACTAA

Upstream 100 bases:

>100_bases
TAAATATAATTTCATTGATTAATTGATAAAACACAAGTAGTGTATAATTGCAGGTTGTAAATCAATATAATCTAATTTAT
TATTTAAAAGGTGGTGTTGT

Downstream 100 bases:

>100_bases
TTTAAATTTTAATTTAAATTTTTAAGATCAAAAAAGAAAAACATTTATTCTAAGTTAATTTCTTCGGAAAATTCTTCGAC
TAAAACATCTTTTTTGCCAC

Product: aspartate kinase

Products: NA

Alternate protein names: Aspartate kinase [H]

Number of amino acids: Translated: 465; Mature: 465

Protein sequence:

>465_residues
MVTVMKFGGTSVGNGERIRNVAKIVVNKTKEDKDVCVVTSAMTQVTNSLIEISTQALDVRDIAKINNFIEDLRIKHEIAI
DQAIFNHEIKTEVSKTIESSINELEKVLVGVSYLGELTPKSKDFILSFGERLSAPILSGAIRDLGKHSLFLTGRDAGIVT
DDSFSCAKVVRLEVLDKVSPILKDGFIPVITGFIGGTDDNQITTFGRGGSDYSAALVGSGLNADMVEIWTDVSGVLSADP
RMVENVKQIPRMSYIEAMELAYFGAKVLHPRTMEPVMEKKIPLRIKNTFDPENEGTLVTDSAETCNSVIKAITTIKDVIL
INIFGGGMVGVSGTAARIFNVLGNSNANVILITQGSSETNISIVIYDGELEAKKCVRELKEEFGECHLIKDITFDKEVCV
VSVVGSGMKGSKGIAGKLFDAVAESGANIKMIAQGSSETNISFVIDEENLENCLKTLHKTFIENN

Sequences:

>Translated_465_residues
MVTVMKFGGTSVGNGERIRNVAKIVVNKTKEDKDVCVVTSAMTQVTNSLIEISTQALDVRDIAKINNFIEDLRIKHEIAI
DQAIFNHEIKTEVSKTIESSINELEKVLVGVSYLGELTPKSKDFILSFGERLSAPILSGAIRDLGKHSLFLTGRDAGIVT
DDSFSCAKVVRLEVLDKVSPILKDGFIPVITGFIGGTDDNQITTFGRGGSDYSAALVGSGLNADMVEIWTDVSGVLSADP
RMVENVKQIPRMSYIEAMELAYFGAKVLHPRTMEPVMEKKIPLRIKNTFDPENEGTLVTDSAETCNSVIKAITTIKDVIL
INIFGGGMVGVSGTAARIFNVLGNSNANVILITQGSSETNISIVIYDGELEAKKCVRELKEEFGECHLIKDITFDKEVCV
VSVVGSGMKGSKGIAGKLFDAVAESGANIKMIAQGSSETNISFVIDEENLENCLKTLHKTFIENN
>Mature_465_residues
MVTVMKFGGTSVGNGERIRNVAKIVVNKTKEDKDVCVVTSAMTQVTNSLIEISTQALDVRDIAKINNFIEDLRIKHEIAI
DQAIFNHEIKTEVSKTIESSINELEKVLVGVSYLGELTPKSKDFILSFGERLSAPILSGAIRDLGKHSLFLTGRDAGIVT
DDSFSCAKVVRLEVLDKVSPILKDGFIPVITGFIGGTDDNQITTFGRGGSDYSAALVGSGLNADMVEIWTDVSGVLSADP
RMVENVKQIPRMSYIEAMELAYFGAKVLHPRTMEPVMEKKIPLRIKNTFDPENEGTLVTDSAETCNSVIKAITTIKDVIL
INIFGGGMVGVSGTAARIFNVLGNSNANVILITQGSSETNISIVIYDGELEAKKCVRELKEEFGECHLIKDITFDKEVCV
VSVVGSGMKGSKGIAGKLFDAVAESGANIKMIAQGSSETNISFVIDEENLENCLKTLHKTFIENN

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=476, Percent_Identity=32.9831932773109, Blast_Score=253, Evalue=2e-68,
Organism=Escherichia coli, GI1790455, Length=467, Percent_Identity=27.4089935760171, Blast_Score=162, Evalue=6e-41,
Organism=Escherichia coli, GI1790376, Length=327, Percent_Identity=26.9113149847095, Blast_Score=103, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6320893, Length=495, Percent_Identity=30.7070707070707, Blast_Score=207, Evalue=4e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR005260
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 50338; Mature: 50338

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTVMKFGGTSVGNGERIRNVAKIVVNKTKEDKDVCVVTSAMTQVTNSLIEISTQALDVR
CEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCHH
DIAKINNFIEDLRIKHEIAIDQAIFNHEIKTEVSKTIESSINELEKVLVGVSYLGELTPK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SKDFILSFGERLSAPILSGAIRDLGKHSLFLTGRDAGIVTDDSFSCAKVVRLEVLDKVSP
CHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEEECCCCEEECCCCCHHHHHHHHHHHHHHH
ILKDGFIPVITGFIGGTDDNQITTFGRGGSDYSAALVGSGLNADMVEIWTDVSGVLSADP
HHHCCCHHHHHHHCCCCCCCCEEEECCCCCCCCHHEECCCCCCHHHHHHHHHHHHHCCCH
RMVENVKQIPRMSYIEAMELAYFGAKVLHPRTMEPVMEKKIPLRIKNTFDPENEGTLVTD
HHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCEEEECCCCCCCCCEEEEC
SAETCNSVIKAITTIKDVILINIFGGGMVGVSGTAARIFNVLGNSNANVILITQGSSETN
CHHHHHHHHHHHHHHHHEEEEEEECCCEECCCCHHHHHHHHHCCCCCCEEEEECCCCCCE
ISIVIYDGELEAKKCVRELKEEFGECHLIKDITFDKEVCVVSVVGSGMKGSKGIAGKLFD
EEEEEECCCCCHHHHHHHHHHHHCCEEEEECCCCCCCEEEEEEECCCCCCCCCCHHHHHH
AVAESGANIKMIAQGSSETNISFVIDEENLENCLKTLHKTFIENN
HHHHCCCCEEEEECCCCCCCEEEEEEHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVTVMKFGGTSVGNGERIRNVAKIVVNKTKEDKDVCVVTSAMTQVTNSLIEISTQALDVR
CEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCHH
DIAKINNFIEDLRIKHEIAIDQAIFNHEIKTEVSKTIESSINELEKVLVGVSYLGELTPK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SKDFILSFGERLSAPILSGAIRDLGKHSLFLTGRDAGIVTDDSFSCAKVVRLEVLDKVSP
CHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEEECCCCEEECCCCCHHHHHHHHHHHHHHH
ILKDGFIPVITGFIGGTDDNQITTFGRGGSDYSAALVGSGLNADMVEIWTDVSGVLSADP
HHHCCCHHHHHHHCCCCCCCCEEEECCCCCCCCHHEECCCCCCHHHHHHHHHHHHHCCCH
RMVENVKQIPRMSYIEAMELAYFGAKVLHPRTMEPVMEKKIPLRIKNTFDPENEGTLVTD
HHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCEEEECCCCCCCCCEEEEC
SAETCNSVIKAITTIKDVILINIFGGGMVGVSGTAARIFNVLGNSNANVILITQGSSETN
CHHHHHHHHHHHHHHHHEEEEEEECCCEECCCCHHHHHHHHHCCCCCCEEEEECCCCCCE
ISIVIYDGELEAKKCVRELKEEFGECHLIKDITFDKEVCVVSVVGSGMKGSKGIAGKLFD
EEEEEECCCCCHHHHHHHHHHHHCCEEEEECCCCCCCEEEEEEECCCCCCCCCCHHHHHH
AVAESGANIKMIAQGSSETNISFVIDEENLENCLKTLHKTFIENN
HHHHCCCCEEEEECCCCCCCEEEEEEHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]