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The map label for this gene is ksgA

Identifier: 150399067

GI number: 150399067

Start: 364543

End: 365346

Strand: Reverse

Name: ksgA

Synonym: Mevan_0313

Alternate gene names: 150399067

Gene position: 365346-364543 (Counterclockwise)

Preceding gene: 150399068

Following gene: 150399066

Centisome position: 21.24

GC content: 29.35

Gene sequence:

>804_bases
ATGAAGCAATCTAAAAAATTAGGGCAGTGTTTTTTAAAAGATAAAAATTTTGTTAAAAAAGCGATTAAAAGTGCGGATAT
AACTGAAAACGATATTGTTTTAGAAGTAGGTCTTGGAGAAGGGGCTCTTACAAAAGAACTTGCTAAACTAGCAAAATTTG
TATACGTAATTGAATTGGATATGCGACTTGAACCTTTTGCAAACCAGATAATGTCTGAATTTAAAAATGTTAAAGTAATT
TGGAATGATGCTCTAAAAGTTGATTTAAAAGAACTTGGATTTAATAAAATCGTTGCAAATCTACCATATCAAATTTCTTC
GCCAATAACTTTTAAGTTTTTAGAAAATGACTTTGACGTTGCAGTTTTAATGTATCAGTACGAATTTGCGAAAAGAATGA
TTGGAAAACCCGATACAGATGAATATAGTAGATTAAGTGTTTCTATACAGTATAACTCCGATGTTGAATTTATTTGTAAG
GTTCCACCCACTGCTTTTTCACCAAAACCGGACGTAAATTCTGCAATTGTAAAACTAACTAAGCGGGAACCTTTATTCCA
TATTGAAAATGAAGAATTTTTTAGAAACGTTTTAAATGCAATATTCCAGCATAGAAATAGAACTGTAAAACGTGCTTTAA
TTGATTCAAGTCATGAAATGAATATTGAACGAGAACGTTTAAAAGAAATTTTAGAAAACATTAAACTTGATTTTGACTTT
TCAGAGCGGGTTTTTAAATTAGCGCCTGAAAAAATTGGGGAATTAAGCAATATATTGTACCTGAATATAATTTCAAAAAA
ATAA

Upstream 100 bases:

>100_bases
TGTGTTTTATCGACCTTTTTCCACACATGAACGCCATAAATTTCATTGAAAAGGCAAAAAAAATCTCTAAACAAGTAGTA
ATTATATAATAAGTGAAATA

Downstream 100 bases:

>100_bases
TGAAATAATGGTTAACAGGTGGTTTAAATGCGAATTATCGTGTTATACTCTGGAAATTACGGTGAACGGGTTTTAAATAA
TATTTTAACCAAATTTGCAC

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N',N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MKQSKKLGQCFLKDKNFVKKAIKSADITENDIVLEVGLGEGALTKELAKLAKFVYVIELDMRLEPFANQIMSEFKNVKVI
WNDALKVDLKELGFNKIVANLPYQISSPITFKFLENDFDVAVLMYQYEFAKRMIGKPDTDEYSRLSVSIQYNSDVEFICK
VPPTAFSPKPDVNSAIVKLTKREPLFHIENEEFFRNVLNAIFQHRNRTVKRALIDSSHEMNIERERLKEILENIKLDFDF
SERVFKLAPEKIGELSNILYLNIISKK

Sequences:

>Translated_267_residues
MKQSKKLGQCFLKDKNFVKKAIKSADITENDIVLEVGLGEGALTKELAKLAKFVYVIELDMRLEPFANQIMSEFKNVKVI
WNDALKVDLKELGFNKIVANLPYQISSPITFKFLENDFDVAVLMYQYEFAKRMIGKPDTDEYSRLSVSIQYNSDVEFICK
VPPTAFSPKPDVNSAIVKLTKREPLFHIENEEFFRNVLNAIFQHRNRTVKRALIDSSHEMNIERERLKEILENIKLDFDF
SERVFKLAPEKIGELSNILYLNIISKK
>Mature_267_residues
MKQSKKLGQCFLKDKNFVKKAIKSADITENDIVLEVGLGEGALTKELAKLAKFVYVIELDMRLEPFANQIMSEFKNVKVI
WNDALKVDLKELGFNKIVANLPYQISSPITFKFLENDFDVAVLMYQYEFAKRMIGKPDTDEYSRLSVSIQYNSDVEFICK
VPPTAFSPKPDVNSAIVKLTKREPLFHIENEEFFRNVLNAIFQHRNRTVKRALIDSSHEMNIERERLKEILENIKLDFDF
SERVFKLAPEKIGELSNILYLNIISKK

Specific function: Specifically dimethylates two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI7657198, Length=248, Percent_Identity=35.0806451612903, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI156415992, Length=244, Percent_Identity=29.5081967213115, Blast_Score=91, Evalue=7e-19,
Organism=Escherichia coli, GI1786236, Length=223, Percent_Identity=27.8026905829596, Blast_Score=101, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI25146882, Length=205, Percent_Identity=35.609756097561, Blast_Score=114, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI25141369, Length=268, Percent_Identity=25, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6324989, Length=230, Percent_Identity=33.0434782608696, Blast_Score=114, Evalue=1e-26,
Organism=Drosophila melanogaster, GI21358017, Length=256, Percent_Identity=32.421875, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI21357273, Length=241, Percent_Identity=26.5560165975104, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_METVS (A6UP00)

Other databases:

- EMBL:   CP000742
- RefSeq:   YP_001322834.1
- ProteinModelPortal:   A6UP00
- SMR:   A6UP00
- STRING:   A6UP00
- GeneID:   5326037
- GenomeReviews:   CP000742_GR
- KEGG:   mvn:Mevan_0313
- eggNOG:   arNOG05894
- HOGENOM:   HBG319664
- OMA:   TPIIMKL
- ProtClustDB:   PRK14896
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: 2.1.1.- [C]

Molecular weight: Translated: 30966; Mature: 30966

Theoretical pI: Translated: 8.60; Mature: 8.60

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 12-12 BINDING 37-37 BINDING 58-58 BINDING 83-83 BINDING 100-100

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQSKKLGQCFLKDKNFVKKAIKSADITENDIVLEVGLGEGALTKELAKLAKFVYVIELD
CCCCHHHHHHHHCCHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHEEEEEC
MRLEPFANQIMSEFKNVKVIWNDALKVDLKELGFNKIVANLPYQISSPITFKFLENDFDV
CCCCHHHHHHHHHHCCEEEEECCCHHHHHHHCCHHHHHHCCCCCCCCCEEEEEECCCCCE
AVLMYQYEFAKRMIGKPDTDEYSRLSVSIQYNSDVEFICKVPPTAFSPKPDVNSAIVKLT
EEHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCEEEEECCCCCCCCCCCCCHHHHEEC
KREPLFHIENEEFFRNVLNAIFQHRNRTVKRALIDSSHEMNIERERLKEILENIKLDFDF
CCCCCEEECCHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCH
SERVFKLAPEKIGELSNILYLNIISKK
HHHHHHHCHHHHHHHHCEEEEEEECCC
>Mature Secondary Structure
MKQSKKLGQCFLKDKNFVKKAIKSADITENDIVLEVGLGEGALTKELAKLAKFVYVIELD
CCCCHHHHHHHHCCHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHEEEEEC
MRLEPFANQIMSEFKNVKVIWNDALKVDLKELGFNKIVANLPYQISSPITFKFLENDFDV
CCCCHHHHHHHHHHCCEEEEECCCHHHHHHHCCHHHHHHCCCCCCCCCEEEEEECCCCCE
AVLMYQYEFAKRMIGKPDTDEYSRLSVSIQYNSDVEFICKVPPTAFSPKPDVNSAIVKLT
EEHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCEEEEECCCCCCCCCCCCCHHHHEEC
KREPLFHIENEEFFRNVLNAIFQHRNRTVKRALIDSSHEMNIERERLKEILENIKLDFDF
CCCCCEEECCHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCH
SERVFKLAPEKIGELSNILYLNIISKK
HHHHHHHCHHHHHHHHCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Dimethylation [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA