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The map label for this gene is cobA [H]

Identifier: 150399043

GI number: 150399043

Start: 340488

End: 341207

Strand: Reverse

Name: cobA [H]

Synonym: Mevan_0289

Alternate gene names: 150399043

Gene position: 341207-340488 (Counterclockwise)

Preceding gene: 150399049

Following gene: 150399041

Centisome position: 19.84

GC content: 33.75

Gene sequence:

>720_bases
ATGAAAGTAATTTTAGTTGGTGCAGGGCCTGGTGACGAAGAATTAATTACACTAAAAGGAATTGAAGCAATAAAAAATGC
AGATGTAATCGTTTATGATGATTTAATAGGGGAAAAATTGCTAAAATATTCTAAAAAAGATGCAGAACTTATCTATGTGG
GTAAACGGAAAGGAAAACATTCACATAAGCAAGAGGAAATAAACAAAATTTTGATAGATAAAGCAAAGGAAAATAAATTG
GTTGTAAGGCTTAAGGGTGGAGATTCTTTTGTTTTTGGCCGAGGGGGCGAGGAAGTTTTAGCACTAAAAGGAGAAGGAAT
AGAATATGAAATGGTTCCAGGAATTACTTCATCAATTGCAGTTCCGGAAATTTTTGGAATTCCTGTAACCCATAGAAAAG
TTTCAACGTCATTTACTGTTGTCACAGGACACGAAGCAGAAGATAAGTCCGAAGGTGAAATGCAAGTTAAATTAAGTGAA
CTAAATGCAGATACGATTGTTATATTAATGGGGATTACAACACTTGAAAAACATGTATCTGAACTTTTGAAAAACCCTAA
ACGGACTTTGGATACGCCTGTTGCAATATTGATGAATGGTTCACGTGAAAATCAAAGAATTGTTCGTGGAACTCTTTCAA
ATATTGTTGATACGTCAAAACTTGAAAATGTATCTCCCCCAGGAATTATAATCATTGGAGATGTTGTTAATATTCTTTAA

Upstream 100 bases:

>100_bases
AAATTCTATTTTAATTAGTATGTATTATTGAATTTAAAGTAAGGTTACGAAACTACGATTAAATATAATATCGTACATAA
AAATTGAAAAAAGGGAAACT

Downstream 100 bases:

>100_bases
AATAAAAAAGAAATCTAATTCCTCCCCCCAAAGGAATTAGATTTTGTCAGGTGTATTCAAAAAAAATAGTAAAAACTAAT
AGACTCCCCTATTATACCCC

Product: uroporphyrin-III C-methyltransferase

Products: NA

Alternate protein names: Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM [H]

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MKVILVGAGPGDEELITLKGIEAIKNADVIVYDDLIGEKLLKYSKKDAELIYVGKRKGKHSHKQEEINKILIDKAKENKL
VVRLKGGDSFVFGRGGEEVLALKGEGIEYEMVPGITSSIAVPEIFGIPVTHRKVSTSFTVVTGHEAEDKSEGEMQVKLSE
LNADTIVILMGITTLEKHVSELLKNPKRTLDTPVAILMNGSRENQRIVRGTLSNIVDTSKLENVSPPGIIIIGDVVNIL

Sequences:

>Translated_239_residues
MKVILVGAGPGDEELITLKGIEAIKNADVIVYDDLIGEKLLKYSKKDAELIYVGKRKGKHSHKQEEINKILIDKAKENKL
VVRLKGGDSFVFGRGGEEVLALKGEGIEYEMVPGITSSIAVPEIFGIPVTHRKVSTSFTVVTGHEAEDKSEGEMQVKLSE
LNADTIVILMGITTLEKHVSELLKNPKRTLDTPVAILMNGSRENQRIVRGTLSNIVDTSKLENVSPPGIIIIGDVVNIL
>Mature_239_residues
MKVILVGAGPGDEELITLKGIEAIKNADVIVYDDLIGEKLLKYSKKDAELIYVGKRKGKHSHKQEEINKILIDKAKENKL
VVRLKGGDSFVFGRGGEEVLALKGEGIEYEMVPGITSSIAVPEIFGIPVTHRKVSTSFTVVTGHEAEDKSEGEMQVKLSE
LNADTIVILMGITTLEKHVSELLKNPKRTLDTPVAILMNGSRENQRIVRGTLSNIVDTSKLENVSPPGIIIIGDVVNIL

Specific function: Catalyzes both methylations at C-2 and C-7 of uroporphyrinogen III leading to precorrin-1 and precorrin-2; their oxidative esterification gives respectively factor I octamethyl ester and sirohydrochlorin [H]

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=239, Percent_Identity=41.8410041841004, Blast_Score=187, Evalue=4e-49,
Organism=Saccharomyces cerevisiae, GI6322922, Length=238, Percent_Identity=31.0924369747899, Blast_Score=115, Evalue=5e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: =2.1.1.107 [H]

Molecular weight: Translated: 26061; Mature: 26061

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVILVGAGPGDEELITLKGIEAIKNADVIVYDDLIGEKLLKYSKKDAELIYVGKRKGKH
CEEEEEECCCCCCCEEEEECHHHHCCCCEEEEHHHHHHHHHHCCCCCCEEEEEECCCCCC
SHKQEEINKILIDKAKENKLVVRLKGGDSFVFGRGGEEVLALKGEGIEYEMVPGITSSIA
CHHHHHHHHHHHCCCCCCEEEEEEECCCEEEEECCCCEEEEEECCCEEEEECCCCCCCCC
VPEIFGIPVTHRKVSTSFTVVTGHEAEDKSEGEMQVKLSELNADTIVILMGITTLEKHVS
CCHHCCCCCCCEEECCEEEEEECCCCCCCCCCEEEEEEEECCCCEEEEEECHHHHHHHHH
ELLKNPKRTLDTPVAILMNGSRENQRIVRGTLSNIVDTSKLENVSPPGIIIIGDVVNIL
HHHHCCHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHCC
>Mature Secondary Structure
MKVILVGAGPGDEELITLKGIEAIKNADVIVYDDLIGEKLLKYSKKDAELIYVGKRKGKH
CEEEEEECCCCCCCEEEEECHHHHCCCCEEEEHHHHHHHHHHCCCCCCEEEEEECCCCCC
SHKQEEINKILIDKAKENKLVVRLKGGDSFVFGRGGEEVLALKGEGIEYEMVPGITSSIA
CHHHHHHHHHHHCCCCCCEEEEEEECCCEEEEECCCCEEEEEECCCEEEEECCCCCCCCC
VPEIFGIPVTHRKVSTSFTVVTGHEAEDKSEGEMQVKLSELNADTIVILMGITTLEKHVS
CCHHCCCCCCCEEECCEEEEEECCCCCCCCCCEEEEEEEECCCCEEEEEECHHHHHHHHH
ELLKNPKRTLDTPVAILMNGSRENQRIVRGTLSNIVDTSKLENVSPPGIIIIGDVVNIL
HHHHCCHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]