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The map label for this gene is cofE [H]

Identifier: 150399006

GI number: 150399006

Start: 295788

End: 296540

Strand: Reverse

Name: cofE [H]

Synonym: Mevan_0252

Alternate gene names: 150399006

Gene position: 296540-295788 (Counterclockwise)

Preceding gene: 150399007

Following gene: 150398998

Centisome position: 17.24

GC content: 34.13

Gene sequence:

>753_bases
ATGATAAAAGAAAGAGTTAAAATGGAAGTAATTGGTTTAGAAATTCCAATAATTACCGGGGATGAAGAATATAGTCTTGC
AGAATTGATTTCAAAATATCCTTTGTTTGATAACGATATTATTGTTATTGCAGAAACTGTTGTATCAAAAAAAGAAAAAA
ATGTGATAGATAAAAGCGATATTATAATATCCGAAAATGCAATTCAGCTCTCTAAAAAATTAGGAAAAGACCCTGAAGTA
GTTCAGGTGATTTTAGATGAATCAAATGAAATTGTAAAGTTAGGCCCTAATTTTATAGTTACCGAAACTAAACACGGTTT
CGTATGTGCAAACAGCGGCGTTGACGAAAGTAACACTTCAAAAGGGATAAAACCCCTACCAAAAAATCCGGATAAAAGTT
CTGATGAAATAAGGGTTGAAATTGAAAAAATAACTGGTAAAAAGGTTGGAGTAATTATTAATGACAGCATGGGGAGACCA
TTTAGGAAAGGTTCTTGCGGTGTTGCAATAGGAGTTAGCGGAGTTACTGCCCTATGGGATAGACGGGGGGAAAAAGATTT
ATTTGAAAGAACATTAAAAACAACCGAAGTTGGGATTTCAGACGAACTTGCAGCTGCAGCATCTGTTGTAATGGGACAAT
CAAATGAAGGAATCCCCCTTGTAATTATAAGAAATGCACCAGTACCTTTTAATGGCGGGACAGGTAAAGACTTAGTTCGA
AAAAAAGAAGAAGACGTTTTTAGAAATTTATAA

Upstream 100 bases:

>100_bases
ATTGTTAATTTACTTAAAAGTTAATTCATAAATAATATAAATTTATAAATAAATAAAATTTTAGATTTTAAATTTAAAAA
AATAGTTTTGGGAAAAGAAC

Downstream 100 bases:

>100_bases
TTTAAATCTATTTTTTTAAAATAAATCTAATTAAGTAGTTAAGTAACTAACTACTTAATTAACTAATTAAAGGGGCCGCT
ATAATAACTGAAATTAAAAG

Product: F420-0--gamma-glutamyl ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MIKERVKMEVIGLEIPIITGDEEYSLAELISKYPLFDNDIIVIAETVVSKKEKNVIDKSDIIISENAIQLSKKLGKDPEV
VQVILDESNEIVKLGPNFIVTETKHGFVCANSGVDESNTSKGIKPLPKNPDKSSDEIRVEIEKITGKKVGVIINDSMGRP
FRKGSCGVAIGVSGVTALWDRRGEKDLFERTLKTTEVGISDELAAAASVVMGQSNEGIPLVIIRNAPVPFNGGTGKDLVR
KKEEDVFRNL

Sequences:

>Translated_250_residues
MIKERVKMEVIGLEIPIITGDEEYSLAELISKYPLFDNDIIVIAETVVSKKEKNVIDKSDIIISENAIQLSKKLGKDPEV
VQVILDESNEIVKLGPNFIVTETKHGFVCANSGVDESNTSKGIKPLPKNPDKSSDEIRVEIEKITGKKVGVIINDSMGRP
FRKGSCGVAIGVSGVTALWDRRGEKDLFERTLKTTEVGISDELAAAASVVMGQSNEGIPLVIIRNAPVPFNGGTGKDLVR
KKEEDVFRNL
>Mature_250_residues
MIKERVKMEVIGLEIPIITGDEEYSLAELISKYPLFDNDIIVIAETVVSKKEKNVIDKSDIIISENAIQLSKKLGKDPEV
VQVILDESNEIVKLGPNFIVTETKHGFVCANSGVDESNTSKGIKPLPKNPDKSSDEIRVEIEKITGKKVGVIINDSMGRP
FRKGSCGVAIGVSGVTALWDRRGEKDLFERTLKTTEVGISDELAAAASVVMGQSNEGIPLVIIRNAPVPFNGGTGKDLVR
KKEEDVFRNL

Specific function: Catalyzes the GTP-dependent successive addition of multiple gamma-linked L-glutamates to the L-lactyl phosphodiester of 7,8-didemethyl-8-hydroxy-5-deazariboflavin (F420-0) to form polyglutamated F420 derivatives [H]

COG id: COG1478

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CofE family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002847
- InterPro:   IPR008225 [H]

Pfam domain/function: PF01996 F420_ligase [H]

EC number: NA

Molecular weight: Translated: 27288; Mature: 27288

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKERVKMEVIGLEIPIITGDEEYSLAELISKYPLFDNDIIVIAETVVSKKEKNVIDKSD
CCCCCEEEEEEEEEEEEEECCCCHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHCCCCCCC
IIISENAIQLSKKLGKDPEVVQVILDESNEIVKLGPNFIVTETKHGFVCANSGVDESNTS
EEEECHHHHHHHHHCCCHHHEEEEECCCCCEEEECCCEEEEECCCCEEEECCCCCCCCCC
KGIKPLPKNPDKSSDEIRVEIEKITGKKVGVIINDSMGRPFRKGSCGVAIGVSGVTALWD
CCCCCCCCCCCCCCCEEEEEEEEECCCEEEEEEECCCCCCCCCCCCCEEEECCHHHHHHH
RRGEKDLFERTLKTTEVGISDELAAAASVVMGQSNEGIPLVIIRNAPVPFNGGTGKDLVR
CCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCHHHHH
KKEEDVFRNL
HHHHHHHHCC
>Mature Secondary Structure
MIKERVKMEVIGLEIPIITGDEEYSLAELISKYPLFDNDIIVIAETVVSKKEKNVIDKSD
CCCCCEEEEEEEEEEEEEECCCCHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHCCCCCCC
IIISENAIQLSKKLGKDPEVVQVILDESNEIVKLGPNFIVTETKHGFVCANSGVDESNTS
EEEECHHHHHHHHHCCCHHHEEEEECCCCCEEEECCCEEEEECCCCEEEECCCCCCCCCC
KGIKPLPKNPDKSSDEIRVEIEKITGKKVGVIINDSMGRPFRKGSCGVAIGVSGVTALWD
CCCCCCCCCCCCCCCEEEEEEEEECCCEEEEEEECCCCCCCCCCCCCEEEECCHHHHHHH
RRGEKDLFERTLKTTEVGISDELAAAASVVMGQSNEGIPLVIIRNAPVPFNGGTGKDLVR
CCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCHHHHH
KKEEDVFRNL
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA