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The map label for this gene is argF

Identifier: 150398938

GI number: 150398938

Start: 212637

End: 213554

Strand: Reverse

Name: argF

Synonym: Mevan_0182

Alternate gene names: 150398938

Gene position: 213554-212637 (Counterclockwise)

Preceding gene: 150398941

Following gene: 150398937

Centisome position: 12.42

GC content: 31.81

Gene sequence:

>918_bases
ATGGATATGCTTACACTTTGGAATTTAGAAAGAGAAGACATGGAAAAAATTTTAGAAGATTCAATTTACTTTAAAAAAAA
TAGGTATGGCCATGATATTTTAAAAAATAAAAATATTGCGCTAATTTTTGAAAGCCCCTCTACTAGAACTAGGATGAGCT
TTGATTTAGCCGTAAATGAACTTGGTGGGCATTCCATGGTGATGAATGAAAATGAAATTCATCTTGGTAAAAAGGAAAGT
ATTAAAGATACTGCAAAAGTAATGAGCAGATTTGTAGATGTAATAGTTGCAAGGGTAAAAAGCCATAAGACTCTAGAAGA
CCTTGCTTTTTATGGAACTGTTCCGGTAATTAATGCGTTATCTGACCTTTCACACCCCTGCCAAGTACTTGCAGACTTAC
TCACAATAAAAGAAAATGGAAAAGACTTTAAAAATTTAAAATTAGCATATTTTGGGGATGGAAATAACGTTTCAAATTCT
TTAATGATTGCAGGAGCGATACTTGGAATGAATATATTTATTGCAACACCTAGAAGCTATGAACCAAACGGTATTTTCGT
TAAAAAAGCACTGGAAATTATTGCAAAATATGGTGAAGGGAGCCTTACGTTAACTGATGACCCAATAGAAGCTTCAAAAA
ATGCAGATGTATTGTATACTGATGTATGGATTAGTATGAGTGATAAAAACAAAGATTTAGAAGATGTAAAAAGGATATTT
CCTAAATTCCAGATAAATTCAAAACTTTTATCGAATGCAAAAAAAGATGCAATGGTTCTTCACTGCCTCCCTGCAAATAG
AGGAATGGAAATAACTGACGAAGTAATTGATTCTAAACAGTCAAAAGTATTTGATCAGGCTGAAAATAGACTTCACGTTC
AAAAAGCAGTTTTAAAGTACGTTTTAAAAGATAAATAA

Upstream 100 bases:

>100_bases
ATTTCTTTATTTATAATTATTATTGTAATACTTATTACTGTATTAGTTTAATAGTATTCATCTGGAATATTTAACTAGTT
TTCGTTAGTGGTGGTAATTT

Downstream 100 bases:

>100_bases
TACTATTTTTTATTCCCTACTTTAAAAAATTTTCTAATTTTCTAATTTTAAAAGTTTATTACTATACAATTCATTTTAAA
ATTACTCTTTTTTAAATTCA

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MDMLTLWNLEREDMEKILEDSIYFKKNRYGHDILKNKNIALIFESPSTRTRMSFDLAVNELGGHSMVMNENEIHLGKKES
IKDTAKVMSRFVDVIVARVKSHKTLEDLAFYGTVPVINALSDLSHPCQVLADLLTIKENGKDFKNLKLAYFGDGNNVSNS
LMIAGAILGMNIFIATPRSYEPNGIFVKKALEIIAKYGEGSLTLTDDPIEASKNADVLYTDVWISMSDKNKDLEDVKRIF
PKFQINSKLLSNAKKDAMVLHCLPANRGMEITDEVIDSKQSKVFDQAENRLHVQKAVLKYVLKDK

Sequences:

>Translated_305_residues
MDMLTLWNLEREDMEKILEDSIYFKKNRYGHDILKNKNIALIFESPSTRTRMSFDLAVNELGGHSMVMNENEIHLGKKES
IKDTAKVMSRFVDVIVARVKSHKTLEDLAFYGTVPVINALSDLSHPCQVLADLLTIKENGKDFKNLKLAYFGDGNNVSNS
LMIAGAILGMNIFIATPRSYEPNGIFVKKALEIIAKYGEGSLTLTDDPIEASKNADVLYTDVWISMSDKNKDLEDVKRIF
PKFQINSKLLSNAKKDAMVLHCLPANRGMEITDEVIDSKQSKVFDQAENRLHVQKAVLKYVLKDK
>Mature_305_residues
MDMLTLWNLEREDMEKILEDSIYFKKNRYGHDILKNKNIALIFESPSTRTRMSFDLAVNELGGHSMVMNENEIHLGKKES
IKDTAKVMSRFVDVIVARVKSHKTLEDLAFYGTVPVINALSDLSHPCQVLADLLTIKENGKDFKNLKLAYFGDGNNVSNS
LMIAGAILGMNIFIATPRSYEPNGIFVKKALEIIAKYGEGSLTLTDDPIEASKNADVLYTDVWISMSDKNKDLEDVKRIF
PKFQINSKLLSNAKKDAMVLHCLPANRGMEITDEVIDSKQSKVFDQAENRLHVQKAVLKYVLKDK

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI38788445, Length=309, Percent_Identity=41.1003236245955, Blast_Score=219, Evalue=3e-57,
Organism=Homo sapiens, GI18105007, Length=276, Percent_Identity=28.9855072463768, Blast_Score=93, Evalue=3e-19,
Organism=Escherichia coli, GI1790703, Length=287, Percent_Identity=39.7212543554007, Blast_Score=208, Evalue=4e-55,
Organism=Escherichia coli, GI1786469, Length=302, Percent_Identity=38.7417218543046, Blast_Score=205, Evalue=4e-54,
Organism=Escherichia coli, GI2367364, Length=311, Percent_Identity=32.7974276527331, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI48994908, Length=353, Percent_Identity=23.2294617563739, Blast_Score=75, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI193204318, Length=339, Percent_Identity=24.4837758112094, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6322373, Length=322, Percent_Identity=34.472049689441, Blast_Score=172, Evalue=8e-44,
Organism=Saccharomyces cerevisiae, GI6322331, Length=276, Percent_Identity=27.8985507246377, Blast_Score=90, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24642586, Length=284, Percent_Identity=28.169014084507, Blast_Score=93, Evalue=2e-19,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): OTC_METVS (A6UNM1)

Other databases:

- EMBL:   CP000742
- RefSeq:   YP_001322705.1
- ProteinModelPortal:   A6UNM1
- SMR:   A6UNM1
- STRING:   A6UNM1
- GeneID:   5325104
- GenomeReviews:   CP000742_GR
- KEGG:   mvn:Mevan_0182
- eggNOG:   arNOG04256
- HOGENOM:   HBG579429
- OMA:   CVVTDTW
- ProtClustDB:   PRK00779
- HAMAP:   MF_01109
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292
- PRINTS:   PR00100
- PRINTS:   PR00102
- TIGRFAMs:   TIGR00658

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.3

Molecular weight: Translated: 34463; Mature: 34463

Theoretical pI: Translated: 7.21; Mature: 7.21

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: BINDING 98-98 BINDING 125-125

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDMLTLWNLEREDMEKILEDSIYFKKNRYGHDILKNKNIALIFESPSTRTRMSFDLAVNE
CCCEEEECCCHHHHHHHHHHHHEECCCCCCCHHHCCCCEEEEECCCCCCEEEEEEEEHHH
LGGHSMVMNENEIHLGKKESIKDTAKVMSRFVDVIVARVKSHKTLEDLAFYGTVPVINAL
CCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCHHHHHHHH
SDLSHPCQVLADLLTIKENGKDFKNLKLAYFGDGNNVSNSLMIAGAILGMNIFIATPRSY
HHCCHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHCCEEEEECCCCC
EPNGIFVKKALEIIAKYGEGSLTLTDDPIEASKNADVLYTDVWISMSDKNKDLEDVKRIF
CCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCEEEEEEEEEECCCCCCHHHHHHHC
PKFQINSKLLSNAKKDAMVLHCLPANRGMEITDEVIDSKQSKVFDQAENRLHVQKAVLKY
CCCCCCHHHHCCCCCCEEEEEEECCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
VLKDK
HHCCC
>Mature Secondary Structure
MDMLTLWNLEREDMEKILEDSIYFKKNRYGHDILKNKNIALIFESPSTRTRMSFDLAVNE
CCCEEEECCCHHHHHHHHHHHHEECCCCCCCHHHCCCCEEEEECCCCCCEEEEEEEEHHH
LGGHSMVMNENEIHLGKKESIKDTAKVMSRFVDVIVARVKSHKTLEDLAFYGTVPVINAL
CCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCHHHHHHHH
SDLSHPCQVLADLLTIKENGKDFKNLKLAYFGDGNNVSNSLMIAGAILGMNIFIATPRSY
HHCCHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHCCEEEEECCCCC
EPNGIFVKKALEIIAKYGEGSLTLTDDPIEASKNADVLYTDVWISMSDKNKDLEDVKRIF
CCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCEEEEEEEEEECCCCCCHHHHHHHC
PKFQINSKLLSNAKKDAMVLHCLPANRGMEITDEVIDSKQSKVFDQAENRLHVQKAVLKY
CCCCCCHHHHCCCCCCEEEEEEECCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
VLKDK
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA