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The map label for this gene is nifD [H]

Identifier: 150398821

GI number: 150398821

Start: 61371

End: 62798

Strand: Reverse

Name: nifD [H]

Synonym: Mevan_0062

Alternate gene names: 150398821

Gene position: 62798-61371 (Counterclockwise)

Preceding gene: 150398822

Following gene: 150398820

Centisome position: 3.65

GC content: 39.57

Gene sequence:

>1428_bases
ATGCCACACTGCTTACTAGATGTAGATAAAGACATCCCCGAAAGAGAGAAGCACGTTTACATCAAAAACTCAAACGAACC
AAGACATTGCCAGCCACACTGTAACAAAAGTACAATCCCCGGAAGTATGACTGAGAGAGGTTGTGCATTTGCAGGAGTTA
AGGGCGTAATAACCGGTGCTTTAAAAGACGTAGCACACGTAGTCCACTCGCCTGTTGGATGCACTGCATACGGAAATGGT
ACAACAAAAAGATACCCAACAAACCCATTAATGCCTGATGGAACCGTATTTCCAATTGAAAACTTCAACCTCAAGCACAT
TATTGGAACTGACTTAAACGAATCCGATGTCGTGTTTGGTGGAATGAACAAGCTTAAAAAAGTAATTAGGGAAGCTGCAA
AAGAATATCCTGAAGCAAATGCAATTTATACCTACGCAACATGTACAACCGGCCTTATCGGGGATGATTTAGATGCAGTA
TGTAAAGAAATGTCCGAAGAACTTGGAAAAGACGTTGTAGCATTCAATGCCCCCGGTTTTGCAGGGCCTACCCAATCAAA
AGGACACCACGTTGGAAATTACACAATATTTTCAAAATTAGTCGGTACAAAAGAGCCACCAAAAACAACGGACTACGACA
TCAACCTGATTGGGGAATATAACATTGATGGTGACTACTGGGTACTTGAAAAATATTTTAAAGAAATGGGTATTAACGTT
CTCAGTAAATTTACTGGAGATGCATGCCACGACGAGCTCTGTTGGATGCACAAAGCAAAATTAAGTCTTGTAAGGTGCCA
AAGATCTGCTACCTACGTTGCAAAATTAATCGAAGAAAAATACGGTGTTCCATATATCAAAGTAGACTTTTTCGGCCCAA
GATACTGTGCAGAAAACTTAATTGCAGTTGGTAAATACTTTGGAAAAGAGGAAGAAGCAAACGCAGTTATCAAAGACAGA
ATGGACAAAATCCAGCCTGAACTCGACTTTTATAAATCAAAATTACAAGGCAAAAAAATCTGGATTTCAGCAGGGGGCCC
AAAAAGCTGGCACTTTGCAGAACCAATTAATGAATTCTTAGGAATGGAAATTGTAGCACTTTCGGGTCTTTTCGAACATG
AAGATGGCTATGAAAAAATGAAAGACAGGGCAAAAGATGGTACAATTATCATTGATGACCCAAACACCCTCGAAATGGAA
GAAATTGTGGAAAAATACAAACCTGAAATTGTTCTTGGCGGCATTAAAGAGAAATATCTGTTCCACAAATTGGGTGTACC
TTCAGTAATGATCCACTCATACGAAAACGGCCCATACATCGGATTTGAAGGATTTGTGAATATGGCAAAAGACATATATT
CAGCAATATATAACCCAGCCTGGAAATTGATGGAATTCCAAGAAGAAGAGCCAGGTGATGCAAAATGA

Upstream 100 bases:

>100_bases
AAGTATGATCAAGACTAAAAAAGAAAATTCATGTCCAAAATAAACTTAAAATAAAATATACAGAATATATTACATTTAAA
AATTTTTTAGGAGTGCTAAA

Downstream 100 bases:

>100_bases
GTAATCTAAACGTAATCAACAAAAACAGGACCGCTGTAATCAACCCGCTCGTAACATGCCAACCATTAGGTGCAATGTTT
GCAGTATCAGGAATTAAACG

Product: nitrogenase subunit alpha

Products: NA

Alternate protein names: Dinitrogenase; Nitrogenase component I [H]

Number of amino acids: Translated: 475; Mature: 474

Protein sequence:

>475_residues
MPHCLLDVDKDIPEREKHVYIKNSNEPRHCQPHCNKSTIPGSMTERGCAFAGVKGVITGALKDVAHVVHSPVGCTAYGNG
TTKRYPTNPLMPDGTVFPIENFNLKHIIGTDLNESDVVFGGMNKLKKVIREAAKEYPEANAIYTYATCTTGLIGDDLDAV
CKEMSEELGKDVVAFNAPGFAGPTQSKGHHVGNYTIFSKLVGTKEPPKTTDYDINLIGEYNIDGDYWVLEKYFKEMGINV
LSKFTGDACHDELCWMHKAKLSLVRCQRSATYVAKLIEEKYGVPYIKVDFFGPRYCAENLIAVGKYFGKEEEANAVIKDR
MDKIQPELDFYKSKLQGKKIWISAGGPKSWHFAEPINEFLGMEIVALSGLFEHEDGYEKMKDRAKDGTIIIDDPNTLEME
EIVEKYKPEIVLGGIKEKYLFHKLGVPSVMIHSYENGPYIGFEGFVNMAKDIYSAIYNPAWKLMEFQEEEPGDAK

Sequences:

>Translated_475_residues
MPHCLLDVDKDIPEREKHVYIKNSNEPRHCQPHCNKSTIPGSMTERGCAFAGVKGVITGALKDVAHVVHSPVGCTAYGNG
TTKRYPTNPLMPDGTVFPIENFNLKHIIGTDLNESDVVFGGMNKLKKVIREAAKEYPEANAIYTYATCTTGLIGDDLDAV
CKEMSEELGKDVVAFNAPGFAGPTQSKGHHVGNYTIFSKLVGTKEPPKTTDYDINLIGEYNIDGDYWVLEKYFKEMGINV
LSKFTGDACHDELCWMHKAKLSLVRCQRSATYVAKLIEEKYGVPYIKVDFFGPRYCAENLIAVGKYFGKEEEANAVIKDR
MDKIQPELDFYKSKLQGKKIWISAGGPKSWHFAEPINEFLGMEIVALSGLFEHEDGYEKMKDRAKDGTIIIDDPNTLEME
EIVEKYKPEIVLGGIKEKYLFHKLGVPSVMIHSYENGPYIGFEGFVNMAKDIYSAIYNPAWKLMEFQEEEPGDAK
>Mature_474_residues
PHCLLDVDKDIPEREKHVYIKNSNEPRHCQPHCNKSTIPGSMTERGCAFAGVKGVITGALKDVAHVVHSPVGCTAYGNGT
TKRYPTNPLMPDGTVFPIENFNLKHIIGTDLNESDVVFGGMNKLKKVIREAAKEYPEANAIYTYATCTTGLIGDDLDAVC
KEMSEELGKDVVAFNAPGFAGPTQSKGHHVGNYTIFSKLVGTKEPPKTTDYDINLIGEYNIDGDYWVLEKYFKEMGINVL
SKFTGDACHDELCWMHKAKLSLVRCQRSATYVAKLIEEKYGVPYIKVDFFGPRYCAENLIAVGKYFGKEEEANAVIKDRM
DKIQPELDFYKSKLQGKKIWISAGGPKSWHFAEPINEFLGMEIVALSGLFEHEDGYEKMKDRAKDGTIIIDDPNTLEMEE
IVEKYKPEIVLGGIKEKYLFHKLGVPSVMIHSYENGPYIGFEGFVNMAKDIYSAIYNPAWKLMEFQEEEPGDAK

Specific function: This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR005974
- InterPro:   IPR010143
- InterPro:   IPR000318 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 53267; Mature: 53136

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: PS00090 NITROGENASE_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHCLLDVDKDIPEREKHVYIKNSNEPRHCQPHCNKSTIPGSMTERGCAFAGVKGVITGA
CCCCEEECCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHH
LKDVAHVVHSPVGCTAYGNGTTKRYPTNPLMPDGTVFPIENFNLKHIIGTDLNESDVVFG
HHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCEEEECCCCCEEEEECCCCCCCCEEEC
GMNKLKKVIREAAKEYPEANAIYTYATCTTGLIGDDLDAVCKEMSEELGKDVVAFNAPGF
CHHHHHHHHHHHHHHCCCCCCEEEEEEHHCCCCCCCHHHHHHHHHHHHCCCEEEECCCCC
AGPTQSKGHHVGNYTIFSKLVGTKEPPKTTDYDINLIGEYNIDGDYWVLEKYFKEMGINV
CCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHCHHH
LSKFTGDACHDELCWMHKAKLSLVRCQRSATYVAKLIEEKYGVPYIKVDFFGPRYCAENL
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHH
IAVGKYFGKEEEANAVIKDRMDKIQPELDFYKSKLQGKKIWISAGGPKSWHFAEPINEFL
HHHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHH
GMEIVALSGLFEHEDGYEKMKDRAKDGTIIIDDPNTLEMEEIVEKYKPEIVLGGIKEKYL
CCCHHHHHHHHHCCCCHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCEEECCCHHHHH
FHKLGVPSVMIHSYENGPYIGFEGFVNMAKDIYSAIYNPAWKLMEFQEEEPGDAK
HHHCCCCEEEEEECCCCCEECHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
PHCLLDVDKDIPEREKHVYIKNSNEPRHCQPHCNKSTIPGSMTERGCAFAGVKGVITGA
CCCEEECCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHH
LKDVAHVVHSPVGCTAYGNGTTKRYPTNPLMPDGTVFPIENFNLKHIIGTDLNESDVVFG
HHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCEEEECCCCCEEEEECCCCCCCCEEEC
GMNKLKKVIREAAKEYPEANAIYTYATCTTGLIGDDLDAVCKEMSEELGKDVVAFNAPGF
CHHHHHHHHHHHHHHCCCCCCEEEEEEHHCCCCCCCHHHHHHHHHHHHCCCEEEECCCCC
AGPTQSKGHHVGNYTIFSKLVGTKEPPKTTDYDINLIGEYNIDGDYWVLEKYFKEMGINV
CCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHCHHH
LSKFTGDACHDELCWMHKAKLSLVRCQRSATYVAKLIEEKYGVPYIKVDFFGPRYCAENL
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHH
IAVGKYFGKEEEANAVIKDRMDKIQPELDFYKSKLQGKKIWISAGGPKSWHFAEPINEFL
HHHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHH
GMEIVALSGLFEHEDGYEKMKDRAKDGTIIIDDPNTLEMEEIVEKYKPEIVLGGIKEKYL
CCCHHHHHHHHHCCCCHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCEEECCCHHHHH
FHKLGVPSVMIHSYENGPYIGFEGFVNMAKDIYSAIYNPAWKLMEFQEEEPGDAK
HHHCCCCEEEEEECCCCCEECHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8990309 [H]