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The map label for this gene is icaA

Identifier: 150395181

GI number: 150395181

Start: 2862353

End: 2863552

Strand: Direct

Name: icaA

Synonym: SaurJH1_2747

Alternate gene names: 150395181

Gene position: 2862353-2863552 (Clockwise)

Preceding gene: 150395173

Following gene: 150395182

Centisome position: 98.48

GC content: 34.75

Gene sequence:

>1200_bases
ATGTCTATTTACTGGATTGTCGGTTCAATTTATTTCTATTTTACCAGAGAAATTAGATATTCATTGAACAAGAAGCCTGA
CATAAATGTGGATGAATTAGAAGGCATTACATTTTTACTTGCCTGTTATAACGAAAGTGAAACGATTGAAGATACGTTGT
CTAATGTTCTTGCACTCAAATACGAGAAGAAAGAAATTATTATCATTAATGATGGAAGTTCAGATAATACAGCAGAACTC
ATCTATAAAATCAAAGAAAATAATGACTTTATTTTCGTCGATTTACAAGAAAACAGAGGTAAAGCCAACGCACTCAATCA
AGGCATTAAACAGGCTTCATATGATTATGTAATGTGCTTGGATGCAGATACTATCGTTGATCAAGATGCACCATATTATA
TGATTGAGAATTTCAAACATGATCCAAAACTTGGTGCAGTTACAGGTAATCCTAGAATTCGAAATAAGAGTTCTATTTTA
GGTAAAATTCAAACGATAGAATATGCAAGTTTAATTGGCTGTATTAAGCGAAGTCAGACACTTGCTGGCGCAGTCAATAC
TATTTCGGGTGTCTTCACTCTATTTAAAAAAAGTGCAGTTGTCGACGTTGGCTACTGGGATACTGATATGATTACCGAAG
ATATTGCAGTTTCTTGGAAATTGCATTTACGTGGATATCGTATTAAGTATGAACCGCTTGCCATGTGTTGGATGTTGGTT
CCAGAAACATTGGGAGGTCTTTGGAAGCAACGCGTGAGATGGGCTCAAGGGGGACACGAAGTATTACTACGAGACTTTTT
TAGCACAATGAAAACGAAAAGGTTTCCTTTATATATTTTGATGTTTGAGCAAATCATCTCAATTTTATGGGTATATATAG
TGCTTCTATATTTAGGCTATTTGTTCATAACAGCAAACTTCTTAGACTATACATTTATGACATATAGTTTTTCAATATTT
CTACTATCATCATTTACTATGACTTTTATAAACGTTATTCAATTTACAGTCGCACTCTTTATTGATAGTCGCTACGAGAA
AAAGAATATGGCTGGACTCATATTTGTAAGTTGGTATCCGACAGTATACTGGATTATTAACGCAGCAGTAGTTCTTGTCG
CATTTCCAAAAGCATTAAAACGTAAGAAAGGTGGTTACGCAACATGGTCAAGCCCAGACAGAGGGAATACCCAACGCTAA

Upstream 100 bases:

>100_bases
TTAAAATACTATCAATTACCATATGGCTTACAACCTAACTAACGAAAGGTAGGTAAAGAAATTGCAATTTTTTAACTTTT
TGCTTTTTTATCCTGTATTT

Downstream 100 bases:

>100_bases
AATCATCGCTAAATATTGTAAGAGAAACAGCACTTATCGCTATATCGTGTGTCTTTTGGATATATTGTTTAGTTGTTCTA
CTCGTTTATATTGGTACTAT

Product: N-glycosyltransferase

Products: NA

Alternate protein names: PNAG synthase; Poly-beta-1,6-GlcNAc synthase; Biofilm polysaccharide intercellular adhesin synthesis protein IcaA; Biofilm PIA synthesis protein IcaA; Intercellular adhesion protein A; N-acetylglucosaminyltransferase IcaA

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MSIYWIVGSIYFYFTREIRYSLNKKPDINVDELEGITFLLACYNESETIEDTLSNVLALKYEKKEIIIINDGSSDNTAEL
IYKIKENNDFIFVDLQENRGKANALNQGIKQASYDYVMCLDADTIVDQDAPYYMIENFKHDPKLGAVTGNPRIRNKSSIL
GKIQTIEYASLIGCIKRSQTLAGAVNTISGVFTLFKKSAVVDVGYWDTDMITEDIAVSWKLHLRGYRIKYEPLAMCWMLV
PETLGGLWKQRVRWAQGGHEVLLRDFFSTMKTKRFPLYILMFEQIISILWVYIVLLYLGYLFITANFLDYTFMTYSFSIF
LLSSFTMTFINVIQFTVALFIDSRYEKKNMAGLIFVSWYPTVYWIINAAVVLVAFPKALKRKKGGYATWSSPDRGNTQR

Sequences:

>Translated_399_residues
MSIYWIVGSIYFYFTREIRYSLNKKPDINVDELEGITFLLACYNESETIEDTLSNVLALKYEKKEIIIINDGSSDNTAEL
IYKIKENNDFIFVDLQENRGKANALNQGIKQASYDYVMCLDADTIVDQDAPYYMIENFKHDPKLGAVTGNPRIRNKSSIL
GKIQTIEYASLIGCIKRSQTLAGAVNTISGVFTLFKKSAVVDVGYWDTDMITEDIAVSWKLHLRGYRIKYEPLAMCWMLV
PETLGGLWKQRVRWAQGGHEVLLRDFFSTMKTKRFPLYILMFEQIISILWVYIVLLYLGYLFITANFLDYTFMTYSFSIF
LLSSFTMTFINVIQFTVALFIDSRYEKKNMAGLIFVSWYPTVYWIINAAVVLVAFPKALKRKKGGYATWSSPDRGNTQR
>Mature_398_residues
SIYWIVGSIYFYFTREIRYSLNKKPDINVDELEGITFLLACYNESETIEDTLSNVLALKYEKKEIIIINDGSSDNTAELI
YKIKENNDFIFVDLQENRGKANALNQGIKQASYDYVMCLDADTIVDQDAPYYMIENFKHDPKLGAVTGNPRIRNKSSILG
KIQTIEYASLIGCIKRSQTLAGAVNTISGVFTLFKKSAVVDVGYWDTDMITEDIAVSWKLHLRGYRIKYEPLAMCWMLVP
ETLGGLWKQRVRWAQGGHEVLLRDFFSTMKTKRFPLYILMFEQIISILWVYIVLLYLGYLFITANFLDYTFMTYSFSIFL
LSSFTMTFINVIQFTVALFIDSRYEKKNMAGLIFVSWYPTVYWIINAAVVLVAFPKALKRKKGGYATWSSPDRGNTQR

Specific function: N-acetylglucosaminyltransferase that catalyzes the polymerization of single monomer units of UDP-N-acetylglucosamine to produce the linear homopolymer poly-beta-1,6-N-acetyl-D- glucosamine (PNAG, also referred to as PIA), a biofilm adhesin polysaccharide.

COG id: COG1215

COG function: function code M; Glycosyltransferases, probably involved in cell wall biogenesis

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 2 family

Homologues:

Organism=Escherichia coli, GI1787259, Length=405, Percent_Identity=39.5061728395062, Blast_Score=332, Evalue=3e-92,
Organism=Escherichia coli, GI1786560, Length=215, Percent_Identity=32.093023255814, Blast_Score=89, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ICAA_STAA8 (Q9RQP9)

Other databases:

- EMBL:   AF086783
- EMBL:   CP000253
- RefSeq:   YP_501451.1
- ProteinModelPortal:   Q9RQP9
- STRING:   Q9RQP9
- EnsemblBacteria:   EBSTAT00000028991
- GeneID:   3921484
- GenomeReviews:   CP000253_GR
- KEGG:   sao:SAOUHSC_03002
- eggNOG:   COG1215
- GeneTree:   EBGT00050000024002
- HOGENOM:   HBG514801
- OMA:   AWAYSMS
- ProtClustDB:   PRK11204
- InterPro:   IPR001173

Pfam domain/function: PF00535 Glycos_transf_2

EC number: NA

Molecular weight: Translated: 46075; Mature: 45944

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x16e34d14)-; HASH(0x190d1144)-; HASH(0x18a1f320)-; HASH(0x186883f8)-;

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIYWIVGSIYFYFTREIRYSLNKKPDINVDELEGITFLLACYNESETIEDTLSNVLALK
CEEEEEEHHHHHHHHHHHHHCCCCCCCCCHHHHCCEEEEEEEECCCCHHHHHHHHHHEEE
YEKKEIIIINDGSSDNTAELIYKIKENNDFIFVDLQENRGKANALNQGIKQASYDYVMCL
ECCCEEEEEECCCCCCCEEEEEEEECCCCEEEEEEECCCCCHHHHHHHHHHCCCCEEEEE
DADTIVDQDAPYYMIENFKHDPKLGAVTGNPRIRNKSSILGKIQTIEYASLIGCIKRSQT
CCHHHCCCCCCCHHHHCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LAGAVNTISGVFTLFKKSAVVDVGYWDTDMITEDIAVSWKLHLRGYRIKYEPLAMCWMLV
HHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHEEEEEEEEEEEEEEEHHHHHHHHHH
PETLGGLWKQRVRWAQGGHEVLLRDFFSTMKTKRFPLYILMFEQIISILWVYIVLLYLGY
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LFITANFLDYTFMTYSFSIFLLSSFTMTFINVIQFTVALFIDSRYEKKNMAGLIFVSWYP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEHHH
TVYWIINAAVVLVAFPKALKRKKGGYATWSSPDRGNTQR
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCH
>Mature Secondary Structure 
SIYWIVGSIYFYFTREIRYSLNKKPDINVDELEGITFLLACYNESETIEDTLSNVLALK
EEEEEEHHHHHHHHHHHHHCCCCCCCCCHHHHCCEEEEEEEECCCCHHHHHHHHHHEEE
YEKKEIIIINDGSSDNTAELIYKIKENNDFIFVDLQENRGKANALNQGIKQASYDYVMCL
ECCCEEEEEECCCCCCCEEEEEEEECCCCEEEEEEECCCCCHHHHHHHHHHCCCCEEEEE
DADTIVDQDAPYYMIENFKHDPKLGAVTGNPRIRNKSSILGKIQTIEYASLIGCIKRSQT
CCHHHCCCCCCCHHHHCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LAGAVNTISGVFTLFKKSAVVDVGYWDTDMITEDIAVSWKLHLRGYRIKYEPLAMCWMLV
HHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHEEEEEEEEEEEEEEEHHHHHHHHHH
PETLGGLWKQRVRWAQGGHEVLLRDFFSTMKTKRFPLYILMFEQIISILWVYIVLLYLGY
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LFITANFLDYTFMTYSFSIFLLSSFTMTFINVIQFTVALFIDSRYEKKNMAGLIFVSWYP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEHHH
TVYWIINAAVVLVAFPKALKRKKGGYATWSSPDRGNTQR
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10496925