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The map label for this gene is leuB [H]

Identifier: 150394573

GI number: 150394573

Start: 2210256

End: 2211302

Strand: Direct

Name: leuB [H]

Synonym: SaurJH1_2132

Alternate gene names: 150394573

Gene position: 2210256-2211302 (Clockwise)

Preceding gene: 150394572

Following gene: 150394574

Centisome position: 76.05

GC content: 35.82

Gene sequence:

>1047_bases
ATGACTTATAACATTGTTGCCCTACCTGGTGATGGAATCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAAT
TATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGC
CTTTAACTGAGAAAACCTTAAATGCGTGTAAAAGAGCAGATGCTATTTTACTGGGTGCAATCGGTGGACCTAAATGGACA
GATCCTAACAATCGACCAGAACAAGGATTATTAAAATTGCGTAAATCCTTAAATTTATTTGCAAATATTCGCCCCACTAC
CGTTGTCAAAGGCGCTAGTTCTTTATCACCTTTAAAGGAAGAACGCGTTGAAGGCACAGATTTAGTTATAGTCCGTGAAT
TGACAAGTGGTATTTATTTTGGAGAACCTAGACATTTTAATAATCACGAGGCCTTAGATTCTCTTACTTATACAAGAGAA
GAAATAGAACGCATTGTTCACGTAGCATTTAAATTGGCCGCTTCAAGACGAGGAAAACTAACATCAGTTGATAAAGAAAA
TGTATTAGCTTCTAGTAAATTGTGGCGCAAAGTCGTAAATGAAGTAAGTCAATTATATCCAGAAGTAACAGTAAATCACT
TACTTGTTGATGCTTGTAGTATGCATTTAATCACAAATCCAAAACAATTTGACGTCATCGTATGTGAAAACTTATTTGGC
GATATTTTAAGTGATGAAGCTTCAGTGATTCCTGGTTCACTTGGTTTATCACCTTCTGCCAGTTTTAGTAACGATGGTCC
AAGATTGTATGAGCCTATTCATGGATCAGCACCAGATATTGCAGGTAAAAACGTTGCCAATCCATTTGGAATGATTCTAT
CTTTAGCGATGTGTTTACGTGAAAGCTTAAATCAACCAGATGCTGCAGATGAATTAGAACAACATATTTATAACATGATT
GAACATGGGCAAACGACAGCAGATTTAGGCGGCAAATTGAATACTACTGATATTTTCGAAATTCTATCTCAAAAATTGAA
TCACTAA

Upstream 100 bases:

>100_bases
TTGGTATTGATCATGATATTTTACAAGCCTCTTGTAAAGCATACGTAGAAGCACATGCTAAATTTGCAGCTGAAAATGTT
GAGAAGGTAGGTAATTAATT

Downstream 100 bases:

>100_bases
GGGGGAGATGTAAATGGGTCAAACATTATTTGACAAGGTGTGGAACAGACATGTGTTATACGGGAAATTGGGCGAACCGC
AACTATTATACATTGATTTA

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 348; Mature: 347

Protein sequence:

>348_residues
MTYNIVALPGDGIGPEILNGSLSLLEIISNKYNFNYQIEHHEFGGASIDTFGEPLTEKTLNACKRADAILLGAIGGPKWT
DPNNRPEQGLLKLRKSLNLFANIRPTTVVKGASSLSPLKEERVEGTDLVIVRELTSGIYFGEPRHFNNHEALDSLTYTRE
EIERIVHVAFKLAASRRGKLTSVDKENVLASSKLWRKVVNEVSQLYPEVTVNHLLVDACSMHLITNPKQFDVIVCENLFG
DILSDEASVIPGSLGLSPSASFSNDGPRLYEPIHGSAPDIAGKNVANPFGMILSLAMCLRESLNQPDAADELEQHIYNMI
EHGQTTADLGGKLNTTDIFEILSQKLNH

Sequences:

>Translated_348_residues
MTYNIVALPGDGIGPEILNGSLSLLEIISNKYNFNYQIEHHEFGGASIDTFGEPLTEKTLNACKRADAILLGAIGGPKWT
DPNNRPEQGLLKLRKSLNLFANIRPTTVVKGASSLSPLKEERVEGTDLVIVRELTSGIYFGEPRHFNNHEALDSLTYTRE
EIERIVHVAFKLAASRRGKLTSVDKENVLASSKLWRKVVNEVSQLYPEVTVNHLLVDACSMHLITNPKQFDVIVCENLFG
DILSDEASVIPGSLGLSPSASFSNDGPRLYEPIHGSAPDIAGKNVANPFGMILSLAMCLRESLNQPDAADELEQHIYNMI
EHGQTTADLGGKLNTTDIFEILSQKLNH
>Mature_347_residues
TYNIVALPGDGIGPEILNGSLSLLEIISNKYNFNYQIEHHEFGGASIDTFGEPLTEKTLNACKRADAILLGAIGGPKWTD
PNNRPEQGLLKLRKSLNLFANIRPTTVVKGASSLSPLKEERVEGTDLVIVRELTSGIYFGEPRHFNNHEALDSLTYTREE
IERIVHVAFKLAASRRGKLTSVDKENVLASSKLWRKVVNEVSQLYPEVTVNHLLVDACSMHLITNPKQFDVIVCENLFGD
ILSDEASVIPGSLGLSPSASFSNDGPRLYEPIHGSAPDIAGKNVANPFGMILSLAMCLRESLNQPDAADELEQHIYNMIE
HGQTTADLGGKLNTTDIFEILSQKLNH

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=350, Percent_Identity=31.1428571428571, Blast_Score=148, Evalue=9e-36,
Organism=Homo sapiens, GI28178821, Length=342, Percent_Identity=28.3625730994152, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI28178816, Length=350, Percent_Identity=28, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI4758582, Length=341, Percent_Identity=28.1524926686217, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI28178838, Length=318, Percent_Identity=29.2452830188679, Blast_Score=109, Evalue=4e-24,
Organism=Homo sapiens, GI28178819, Length=185, Percent_Identity=31.3513513513514, Blast_Score=100, Evalue=3e-21,
Organism=Escherichia coli, GI87081683, Length=353, Percent_Identity=47.0254957507082, Blast_Score=336, Evalue=1e-93,
Organism=Escherichia coli, GI1788101, Length=363, Percent_Identity=34.7107438016529, Blast_Score=188, Evalue=6e-49,
Organism=Escherichia coli, GI1787381, Length=374, Percent_Identity=25.9358288770053, Blast_Score=100, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71986051, Length=335, Percent_Identity=30.7462686567164, Blast_Score=137, Evalue=8e-33,
Organism=Caenorhabditis elegans, GI25144293, Length=351, Percent_Identity=30.1994301994302, Blast_Score=132, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17505779, Length=352, Percent_Identity=29.8295454545455, Blast_Score=124, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI17550882, Length=350, Percent_Identity=29.1428571428571, Blast_Score=117, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6319830, Length=357, Percent_Identity=45.3781512605042, Blast_Score=292, Evalue=5e-80,
Organism=Saccharomyces cerevisiae, GI6322097, Length=368, Percent_Identity=34.2391304347826, Blast_Score=164, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6324709, Length=353, Percent_Identity=34.2776203966006, Blast_Score=141, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6324291, Length=358, Percent_Identity=30.4469273743017, Blast_Score=132, Evalue=9e-32,
Organism=Drosophila melanogaster, GI24643268, Length=360, Percent_Identity=33.0555555555556, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24643270, Length=361, Percent_Identity=33.5180055401662, Blast_Score=159, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24661184, Length=357, Percent_Identity=29.6918767507003, Blast_Score=128, Evalue=5e-30,
Organism=Drosophila melanogaster, GI281362242, Length=340, Percent_Identity=29.1176470588235, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24648872, Length=340, Percent_Identity=29.1176470588235, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI161078635, Length=364, Percent_Identity=28.021978021978, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI161078633, Length=364, Percent_Identity=28.021978021978, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24650122, Length=364, Percent_Identity=28.021978021978, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI161078637, Length=364, Percent_Identity=28.021978021978, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI161078639, Length=361, Percent_Identity=28.2548476454294, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI20130355, Length=350, Percent_Identity=22.5714285714286, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 38213; Mature: 38082

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYNIVALPGDGIGPEILNGSLSLLEIISNKYNFNYQIEHHEFGGASIDTFGEPLTEKTL
CCEEEEEECCCCCCHHHHCCHHHHHHHHHCCCCCEEEEEECCCCCCCHHHCCCHHHHHHH
NACKRADAILLGAIGGPKWTDPNNRPEQGLLKLRKSLNLFANIRPTTVVKGASSLSPLKE
HHHHHCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHEECCCHHCCHHHH
ERVEGTDLVIVRELTSGIYFGEPRHFNNHEALDSLTYTREEIERIVHVAFKLAASRRGKL
HHCCCCCEEEEEEHHCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
TSVDKENVLASSKLWRKVVNEVSQLYPEVTVNHLLVDACSMHLITNPKQFDVIVCENLFG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHH
DILSDEASVIPGSLGLSPSASFSNDGPRLYEPIHGSAPDIAGKNVANPFGMILSLAMCLR
HHHCCCCHHCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
ESLNQPDAADELEQHIYNMIEHGQTTADLGGKLNTTDIFEILSQKLNH
HHCCCCCHHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
TYNIVALPGDGIGPEILNGSLSLLEIISNKYNFNYQIEHHEFGGASIDTFGEPLTEKTL
CEEEEEECCCCCCHHHHCCHHHHHHHHHCCCCCEEEEEECCCCCCCHHHCCCHHHHHHH
NACKRADAILLGAIGGPKWTDPNNRPEQGLLKLRKSLNLFANIRPTTVVKGASSLSPLKE
HHHHHCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHEECCCHHCCHHHH
ERVEGTDLVIVRELTSGIYFGEPRHFNNHEALDSLTYTREEIERIVHVAFKLAASRRGKL
HHCCCCCEEEEEEHHCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
TSVDKENVLASSKLWRKVVNEVSQLYPEVTVNHLLVDACSMHLITNPKQFDVIVCENLFG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHH
DILSDEASVIPGSLGLSPSASFSNDGPRLYEPIHGSAPDIAGKNVANPFGMILSLAMCLR
HHHCCCCHHCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
ESLNQPDAADELEQHIYNMIEHGQTTADLGGKLNTTDIFEILSQKLNH
HHCCCCCHHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA