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The map label for this gene is yafV [C]

Identifier: 150394550

GI number: 150394550

Start: 2183913

End: 2184698

Strand: Direct

Name: yafV [C]

Synonym: SaurJH1_2108

Alternate gene names: 150394550

Gene position: 2183913-2184698 (Clockwise)

Preceding gene: 150394549

Following gene: 150394552

Centisome position: 75.14

GC content: 32.06

Gene sequence:

>786_bases
ATGAAAGTCCAAATTTATCAATTACCGATTATTTTTGGAGATAGTTCTAAAAATGAAACTCAAATAACGCAATGGTTCGA
AAAAAATATGAATGCTGAAGTAGATGTAGTCGTTTTACCTGAAATGTGGAATAATGGCTATGATTTAGAGCATTTAAATG
AAAAAGCAGATAATAATCTAGGACAAAGTTTTTCATTTATTAAACATTTAGCTGAAAAATATAAAGTCGATATCGTAGCT
GGGTCAGTTTCTAACATTAGAAACTATCAAATATTTAATACAGCTTTTAGTGTGAATAAAAGCGGCCAGCTGATTAATGA
ATACGACAAAGTTCATTTAGTTCCTATGTTAAGAGAGCATGAATTTTTAACCGCTGGCGAAAACGTAGCTGAACCCTTTC
AATTGTCTGACGGTACTTATGTGACCCAACTTATCTGTTATGATTTACGTTTTCCAGAATTATTAAGATATCCTGCTCGT
AGTGGTGCTAAAATTGCATTTTATGTGGCGCAATGGCCTATGTCACGTCTTCAACATTGGCATTCATTACTAAAGGCACG
TGCAATTGAAAATAATATGTTTGTCATTGGAACTAATAGCACTGGATTCGATGGTAACACAGAATATGCTGGTCATTCGA
TAGTTATAAACCCAAATGGAGATTTAGTTGGAGAATTAAATGAATCCGCAGATATTTTGACTGTAGATTTAAACTTAAAT
GAAGTAGAACAGCAACGCGAAAATATACCTGTATTTAAAAGTATTAAATTAGATTTGTATAAATAA

Upstream 100 bases:

>100_bases
GTAGAGCCTAGGACTTTGATTTTATGTCTTGGGCTCGTTTTTTGCGTTTCAACATAACTTTTAGTAAGTTATATATAAGA
TCTATCAAGGATGCGATGTT

Downstream 100 bases:

>100_bases
GAAGCGCCCGAAATAATATTTAACACATGTCATGACTAAACATAGATTTATGAGAGATTTTTCATAAGTCTATGTTTATT
TTTTTATAATAGATAACTCA

Product: Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase

Products: NA

Alternate protein names: ORF 5 [H]

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MKVQIYQLPIIFGDSSKNETQITQWFEKNMNAEVDVVVLPEMWNNGYDLEHLNEKADNNLGQSFSFIKHLAEKYKVDIVA
GSVSNIRNYQIFNTAFSVNKSGQLINEYDKVHLVPMLREHEFLTAGENVAEPFQLSDGTYVTQLICYDLRFPELLRYPAR
SGAKIAFYVAQWPMSRLQHWHSLLKARAIENNMFVIGTNSTGFDGNTEYAGHSIVINPNGDLVGELNESADILTVDLNLN
EVEQQRENIPVFKSIKLDLYK

Sequences:

>Translated_261_residues
MKVQIYQLPIIFGDSSKNETQITQWFEKNMNAEVDVVVLPEMWNNGYDLEHLNEKADNNLGQSFSFIKHLAEKYKVDIVA
GSVSNIRNYQIFNTAFSVNKSGQLINEYDKVHLVPMLREHEFLTAGENVAEPFQLSDGTYVTQLICYDLRFPELLRYPAR
SGAKIAFYVAQWPMSRLQHWHSLLKARAIENNMFVIGTNSTGFDGNTEYAGHSIVINPNGDLVGELNESADILTVDLNLN
EVEQQRENIPVFKSIKLDLYK
>Mature_261_residues
MKVQIYQLPIIFGDSSKNETQITQWFEKNMNAEVDVVVLPEMWNNGYDLEHLNEKADNNLGQSFSFIKHLAEKYKVDIVA
GSVSNIRNYQIFNTAFSVNKSGQLINEYDKVHLVPMLREHEFLTAGENVAEPFQLSDGTYVTQLICYDLRFPELLRYPAR
SGAKIAFYVAQWPMSRLQHWHSLLKARAIENNMFVIGTNSTGFDGNTEYAGHSIVINPNGDLVGELNESADILTVDLNLN
EVEQQRENIPVFKSIKLDLYK

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=257, Percent_Identity=28.4046692607004, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI297632350, Length=182, Percent_Identity=30.7692307692308, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI297632348, Length=182, Percent_Identity=30.7692307692308, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI5031947, Length=182, Percent_Identity=30.7692307692308, Blast_Score=96, Evalue=4e-20,
Organism=Escherichia coli, GI1786412, Length=257, Percent_Identity=32.6848249027237, Blast_Score=119, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17556280, Length=264, Percent_Identity=29.1666666666667, Blast_Score=104, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6323383, Length=277, Percent_Identity=29.6028880866426, Blast_Score=125, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6322335, Length=302, Percent_Identity=22.8476821192053, Blast_Score=74, Evalue=3e-14,
Organism=Drosophila melanogaster, GI21355835, Length=239, Percent_Identity=32.6359832635983, Blast_Score=118, Evalue=4e-27,
Organism=Drosophila melanogaster, GI17933642, Length=258, Percent_Identity=26.7441860465116, Blast_Score=96, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010
- InterPro:   IPR001110 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: 3.5.-.- [C]

Molecular weight: Translated: 29840; Mature: 29840

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS50263 CN_HYDROLASE ; PS01227 UPF0012

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVQIYQLPIIFGDSSKNETQITQWFEKNMNAEVDVVVLPEMWNNGYDLEHLNEKADNNL
CEEEEEEEEEEECCCCCCHHHHHHHHHHCCCCEEEEEECHHHCCCCCCHHHHHHHHCCCC
GQSFSFIKHLAEKYKVDIVAGSVSNIRNYQIFNTAFSVNKSGQLINEYDKVHLVPMLREH
CHHHHHHHHHHHHHEEEEEECCHHCCCCEEEEEEEEECCCCCCHHHHCCCEEEEEHHHCC
EFLTAGENVAEPFQLSDGTYVTQLICYDLRFPELLRYPARSGAKIAFYVAQWPMSRLQHW
CEEECCCCCCCCEECCCCCEEEEEEHHCCCCHHHHHCCCCCCCEEEEEEECCCHHHHHHH
HSLLKARAIENNMFVIGTNSTGFDGNTEYAGHSIVINPNGDLVGELNESADILTVDLNLN
HHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCEEEECCCCCEEEECCCCCCEEEEECCHH
EVEQQRENIPVFKSIKLDLYK
HHHHHHHCCCEEEEEEEEECC
>Mature Secondary Structure
MKVQIYQLPIIFGDSSKNETQITQWFEKNMNAEVDVVVLPEMWNNGYDLEHLNEKADNNL
CEEEEEEEEEEECCCCCCHHHHHHHHHHCCCCEEEEEECHHHCCCCCCHHHHHHHHCCCC
GQSFSFIKHLAEKYKVDIVAGSVSNIRNYQIFNTAFSVNKSGQLINEYDKVHLVPMLREH
CHHHHHHHHHHHHHEEEEEECCHHCCCCEEEEEEEEECCCCCCHHHHCCCEEEEEHHHCC
EFLTAGENVAEPFQLSDGTYVTQLICYDLRFPELLRYPARSGAKIAFYVAQWPMSRLQHW
CEEECCCCCCCCEECCCCCEEEEEEHHCCCCHHHHHCCCCCCCEEEEEEECCCHHHHHHH
HSLLKARAIENNMFVIGTNSTGFDGNTEYAGHSIVINPNGDLVGELNESADILTVDLNLN
HHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCEEEECCCCCEEEECCCCCCEEEEECCHH
EVEQQRENIPVFKSIKLDLYK
HHHHHHHCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7565609 [H]