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The map label for this gene is queF

Identifier: 150010473

GI number: 150010473

Start: 4743176

End: 4743649

Strand: Direct

Name: queF

Synonym: BDI_3913

Alternate gene names: 150010473

Gene position: 4743176-4743649 (Clockwise)

Preceding gene: 150010472

Following gene: 150010474

Centisome position: 98.58

GC content: 40.93

Gene sequence:

>474_bases
ATGAAAGAAATGGACGAGAGAAAACAAGAAGGAGAGTTGCATTTATTAGGTGGCTCTACCGTATATAAACAGGATTACGC
TCCGGAGGTATTGGAGGCATTTACGAACAAGCATCAAGGAAATGATTATTGGGTACGGTTTAATTGCCCGGAATTCACCA
GTCTCTGCCCGATTACCGGACAACCGGATTTCGCTACGATTCATATCGATTATATCCCCGACGTGAAAATGGTAGAGAGC
AAGAGCTTGAAGCTTTACTTATTCAGCTTCCGCAACCATGGGGCGTTTCATGAGGATTGCGTGAATATTATCATGAAGGA
CTTGATAAAATTAATGGATCCGAAATATATCGAAATTACTGGTATATTCACACCGAGAGGCGGTATAAGTATTTATCCGT
ATTGCAATTACGGTCGTCCGAACACCAAGTTTGAGCAGTTGGCCGAGAAAAGATTATTTGATCATAATCAATAA

Upstream 100 bases:

>100_bases
CGGTGTTATGGCTGAGGGATGTGGGCATTGTCCGGCTTGCAAACTTCGTAAGGACGGATTGGAGAAATATCTGAAAAGGA
AAGAAGAGAATCAAAAGTAA

Downstream 100 bases:

>100_bases
GATTTCTGACAAATCGTTATCAGTCTCGGCAAAAAACGGTATATTTGCAGCAAGAAATGTTAACTAACTAATAAATCATT
TTAGACCATGAACAAAAGAT

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase

Number of amino acids: Translated: 157; Mature: 157

Protein sequence:

>157_residues
MKEMDERKQEGELHLLGGSTVYKQDYAPEVLEAFTNKHQGNDYWVRFNCPEFTSLCPITGQPDFATIHIDYIPDVKMVES
KSLKLYLFSFRNHGAFHEDCVNIIMKDLIKLMDPKYIEITGIFTPRGGISIYPYCNYGRPNTKFEQLAEKRLFDHNQ

Sequences:

>Translated_157_residues
MKEMDERKQEGELHLLGGSTVYKQDYAPEVLEAFTNKHQGNDYWVRFNCPEFTSLCPITGQPDFATIHIDYIPDVKMVES
KSLKLYLFSFRNHGAFHEDCVNIIMKDLIKLMDPKYIEITGIFTPRGGISIYPYCNYGRPNTKFEQLAEKRLFDHNQ
>Mature_157_residues
MKEMDERKQEGELHLLGGSTVYKQDYAPEVLEAFTNKHQGNDYWVRFNCPEFTSLCPITGQPDFATIHIDYIPDVKMVES
KSLKLYLFSFRNHGAFHEDCVNIIMKDLIKLMDPKYIEITGIFTPRGGISIYPYCNYGRPNTKFEQLAEKRLFDHNQ

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1)

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1789158, Length=91, Percent_Identity=37.3626373626374, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEF_PARD8 (A6LIT0)

Other databases:

- EMBL:   CP000140
- RefSeq:   YP_001305216.1
- ProteinModelPortal:   A6LIT0
- SMR:   A6LIT0
- STRING:   A6LIT0
- GeneID:   5309062
- GenomeReviews:   CP000140_GR
- KEGG:   pdi:BDI_3913
- eggNOG:   COG0780
- HOGENOM:   HBG294103
- OMA:   CINTILL
- ProtClustDB:   PRK13258
- BioCyc:   PDIS435591:BDI_3913-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00818
- InterPro:   IPR016856
- InterPro:   IPR020602
- PIRSF:   PIRSF027377
- TIGRFAMs:   TIGR03139

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =1.7.1.13

Molecular weight: Translated: 18335; Mature: 18335

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEMDERKQEGELHLLGGSTVYKQDYAPEVLEAFTNKHQGNDYWVRFNCPEFTSLCPITG
CCCHHHHHHCCCEEEECCCEEECCCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCC
QPDFATIHIDYIPDVKMVESKSLKLYLFSFRNHGAFHEDCVNIIMKDLIKLMDPKYIEIT
CCCEEEEEEECCCCEEEECCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEE
GIFTPRGGISIYPYCNYGRPNTKFEQLAEKRLFDHNQ
EEECCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKEMDERKQEGELHLLGGSTVYKQDYAPEVLEAFTNKHQGNDYWVRFNCPEFTSLCPITG
CCCHHHHHHCCCEEEECCCEEECCCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCC
QPDFATIHIDYIPDVKMVESKSLKLYLFSFRNHGAFHEDCVNIIMKDLIKLMDPKYIEIT
CCCEEEEEEECCCCEEEECCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEE
GIFTPRGGISIYPYCNYGRPNTKFEQLAEKRLFDHNQ
EEECCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA