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The map label for this gene is rnhB

Identifier: 150010125

GI number: 150010125

Start: 4340838

End: 4341437

Strand: Direct

Name: rnhB

Synonym: BDI_3545

Alternate gene names: 150010125

Gene position: 4340838-4341437 (Clockwise)

Preceding gene: 150010124

Following gene: 150010133

Centisome position: 90.22

GC content: 47.33

Gene sequence:

>600_bases
ATGTTATTACCATATATGGAAACCGGGCGTATAGAGGCCGGGTGTGATGAGGCGGGAAGAGGCTGCTTGGCGGGTGCGGT
ATTCGCCGCCGCCGTGATTCTTCCGGAAGATTTTAAGAATGAGGATTTAAACGATAGCAAGCAATTAAGCGAGAAGAAAC
GGTATAAGCTTCGTCCTGTCATCGAACGTGAGGCGATCGCTTGGGCGGTAGGCATCGTCAGTCCGGAAGAGATCGATAAG
ATTAATATCTTAAAGGCTTCTTTCTTGGCTATGCATCGTGCGATCGAGCAACTGAAAGTACGGCCGGAACATTTATTGAT
CGATGGAAACCGTTTTACCCCTTATCCCGATATTCCGCATACGACCGTAGTAAAAGGCGATGGCAAATACCTGAGTATCG
CCGCCGCCTCTATATTGGCTAAAACCTATCGTGATGATTATATGAATGAATTGGCCTTGGAATACCCCGCTTATCATTGG
CTGGACAATAAAGGTTATCCTACCAAGGTCCACAGGGAAGCGATTCGTACCCACGGAATCACGCCTTATCATCGCAAGAC
ATTCACGCTTTTACCGGAGCAGCTTACACTAGGTTTTTAA

Upstream 100 bases:

>100_bases
CCTTGGATTGTGAATGAGCAAAAATAATGTACAACCTTGTAGAGACGGGGCGTGCTCCGTCTCTACAAAGCTATATATAC
GATAAATAATAAACTGGGAT

Downstream 100 bases:

>100_bases
TACGTATATAGGCATAACCTTATGCGTATATACGTATGAGGCTATGCCTATATCGGTGTAGGCTTACACGTATATACGTA
TCGGCTCGTGCCTATATACG

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 199; Mature: 199

Protein sequence:

>199_residues
MLLPYMETGRIEAGCDEAGRGCLAGAVFAAAVILPEDFKNEDLNDSKQLSEKKRYKLRPVIEREAIAWAVGIVSPEEIDK
INILKASFLAMHRAIEQLKVRPEHLLIDGNRFTPYPDIPHTTVVKGDGKYLSIAAASILAKTYRDDYMNELALEYPAYHW
LDNKGYPTKVHREAIRTHGITPYHRKTFTLLPEQLTLGF

Sequences:

>Translated_199_residues
MLLPYMETGRIEAGCDEAGRGCLAGAVFAAAVILPEDFKNEDLNDSKQLSEKKRYKLRPVIEREAIAWAVGIVSPEEIDK
INILKASFLAMHRAIEQLKVRPEHLLIDGNRFTPYPDIPHTTVVKGDGKYLSIAAASILAKTYRDDYMNELALEYPAYHW
LDNKGYPTKVHREAIRTHGITPYHRKTFTLLPEQLTLGF
>Mature_199_residues
MLLPYMETGRIEAGCDEAGRGCLAGAVFAAAVILPEDFKNEDLNDSKQLSEKKRYKLRPVIEREAIAWAVGIVSPEEIDK
INILKASFLAMHRAIEQLKVRPEHLLIDGNRFTPYPDIPHTTVVKGDGKYLSIAAASILAKTYRDDYMNELALEYPAYHW
LDNKGYPTKVHREAIRTHGITPYHRKTFTLLPEQLTLGF

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Escherichia coli, GI1786380, Length=190, Percent_Identity=47.3684210526316, Blast_Score=164, Evalue=3e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_PARD8 (A6LHT2)

Other databases:

- EMBL:   CP000140
- RefSeq:   YP_001304868.1
- ProteinModelPortal:   A6LHT2
- SMR:   A6LHT2
- STRING:   A6LHT2
- GeneID:   5308694
- GenomeReviews:   CP000140_GR
- KEGG:   pdi:BDI_3545
- eggNOG:   COG0164
- HOGENOM:   HBG584843
- OMA:   RLGPTPI
- ProtClustDB:   PRK00015
- BioCyc:   PDIS435591:BDI_3545-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00052_B
- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337
- PANTHER:   PTHR10954

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 22538; Mature: 22538

Theoretical pI: Translated: 7.21; Mature: 7.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLPYMETGRIEAGCDEAGRGCLAGAVFAAAVILPEDFKNEDLNDSKQLSEKKRYKLRPV
CCCCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCH
IEREAIAWAVGIVSPEEIDKINILKASFLAMHRAIEQLKVRPEHLLIDGNRFTPYPDIPH
HHHHHHHHEEECCCHHHHCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCC
TTVVKGDGKYLSIAAASILAKTYRDDYMNELALEYPAYHWLDNKGYPTKVHREAIRTHGI
CEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHCCC
TPYHRKTFTLLPEQLTLGF
CCCCCCCEEECCHHHHCCC
>Mature Secondary Structure
MLLPYMETGRIEAGCDEAGRGCLAGAVFAAAVILPEDFKNEDLNDSKQLSEKKRYKLRPV
CCCCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCH
IEREAIAWAVGIVSPEEIDKINILKASFLAMHRAIEQLKVRPEHLLIDGNRFTPYPDIPH
HHHHHHHHEEECCCHHHHCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCC
TTVVKGDGKYLSIAAASILAKTYRDDYMNELALEYPAYHWLDNKGYPTKVHREAIRTHGI
CEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHCCC
TPYHRKTFTLLPEQLTLGF
CCCCCCCEEECCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA