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The map label for this gene is algU [H]

Identifier: 150010042

GI number: 150010042

Start: 4220434

End: 4220982

Strand: Direct

Name: algU [H]

Synonym: BDI_3461

Alternate gene names: 150010042

Gene position: 4220434-4220982 (Clockwise)

Preceding gene: 150010041

Following gene: 150010043

Centisome position: 87.72

GC content: 50.64

Gene sequence:

>549_bases
ATGGATGAACAACTGTTAATCGATGGATGTATGCGAGGCGAATCATGGGCTCGTAAGGAATTGTACGAGCAATATGCTCC
TGCTATGATGAGTGTTTGCATGCGTTACGTGGGTGACAGGGAGACGGCCCGTGATCTTCTGCAGGACGGGTTCGTGAAGA
TATTTACGAAGATCCGGTCGTATTCCGGCTCCGGGGCTTTCGGGGGGTGGATACGGCGGATCTTTGTCACTACGGCTCTT
GAATATCTCCGGTCTTCAGAGAACACGAGGCTAAGTGTAGGTTTGGATGGCTATGACGACAGTTTGGAAGATATCAACTA
CTCCGTATTGGACCGTTTATCGGCGGACGACTTGTTGGCTTGCGTGGCCCAATTGCCGCCGGGATATCGTACGGTCTTCA
ACTTATATGCGATAGAGGGATATACGCATAGCGAGATCGCCGAGATGCTGCATGTACAGGAGGCGACTTCCCGCTCCCAG
TTTATCCGGGCGAGGAACGCTTTGCAAAAGAGTGTACAATCTTTAATAAGGCATGAAAATGCGGGATGA

Upstream 100 bases:

>100_bases
CACTCTCCTCCTATTTTTGATGTGAAGGTAACTTTTTTTTGAATAAGATGCAACTTTTAGGATACCTATTTCATCCATAT
AATAGAGAGCATAAGTACTT

Downstream 100 bases:

>100_bases
TAAGGATCATAACGAACGGCTGGATGATTTCAGCGGCTATATGCGGCAGCGCTTGGAAGAGCACCGGTTGCCGGTAGATG
CCGGCTGCTGGGATGAGGTG

Product: putative RNA polymerase ECF-type sigma factor

Products: NA

Alternate protein names: Sigma-30 [H]

Number of amino acids: Translated: 182; Mature: 182

Protein sequence:

>182_residues
MDEQLLIDGCMRGESWARKELYEQYAPAMMSVCMRYVGDRETARDLLQDGFVKIFTKIRSYSGSGAFGGWIRRIFVTTAL
EYLRSSENTRLSVGLDGYDDSLEDINYSVLDRLSADDLLACVAQLPPGYRTVFNLYAIEGYTHSEIAEMLHVQEATSRSQ
FIRARNALQKSVQSLIRHENAG

Sequences:

>Translated_182_residues
MDEQLLIDGCMRGESWARKELYEQYAPAMMSVCMRYVGDRETARDLLQDGFVKIFTKIRSYSGSGAFGGWIRRIFVTTAL
EYLRSSENTRLSVGLDGYDDSLEDINYSVLDRLSADDLLACVAQLPPGYRTVFNLYAIEGYTHSEIAEMLHVQEATSRSQ
FIRARNALQKSVQSLIRHENAG
>Mature_182_residues
MDEQLLIDGCMRGESWARKELYEQYAPAMMSVCMRYVGDRETARDLLQDGFVKIFTKIRSYSGSGAFGGWIRRIFVTTAL
EYLRSSENTRLSVGLDGYDDSLEDINYSVLDRLSADDLLACVAQLPPGYRTVFNLYAIEGYTHSEIAEMLHVQEATSRSQ
FIRARNALQKSVQSLIRHENAG

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor regulates genes such as algD, involved in alginate biosynthesis [H]

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=188, Percent_Identity=27.6595744680851, Blast_Score=72, Evalue=2e-14,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR014286
- InterPro:   IPR013325
- InterPro:   IPR013324 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 20655; Mature: 20655

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDEQLLIDGCMRGESWARKELYEQYAPAMMSVCMRYVGDRETARDLLQDGFVKIFTKIRS
CCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
YSGSGAFGGWIRRIFVTTALEYLRSSENTRLSVGLDGYDDSLEDINYSVLDRLSADDLLA
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHCCHHHHHHCCHHHHHH
CVAQLPPGYRTVFNLYAIEGYTHSEIAEMLHVQEATSRSQFIRARNALQKSVQSLIRHEN
HHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
AG
CC
>Mature Secondary Structure
MDEQLLIDGCMRGESWARKELYEQYAPAMMSVCMRYVGDRETARDLLQDGFVKIFTKIRS
CCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
YSGSGAFGGWIRRIFVTTALEYLRSSENTRLSVGLDGYDDSLEDINYSVLDRLSADDLLA
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHCCHHHHHHCCHHHHHH
CVAQLPPGYRTVFNLYAIEGYTHSEIAEMLHVQEATSRSQFIRARNALQKSVQSLIRHEN
HHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
AG
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8432708; 8378309; 7961421; 10984043; 7737518 [H]