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The map label for this gene is act [H]

Identifier: 150009974

GI number: 150009974

Start: 4126902

End: 4127636

Strand: Direct

Name: act [H]

Synonym: BDI_3393

Alternate gene names: 150009974

Gene position: 4126902-4127636 (Clockwise)

Preceding gene: 150009973

Following gene: 150009975

Centisome position: 85.77

GC content: 45.31

Gene sequence:

>735_bases
GTGATAAAAGGTAAGATACATTCATTAGAGAGTTTCGGGACCGTAGATGGTCCCGGAATTCGCTTTGTCGTTTTTATGCA
GGGCTGCCCGTTGCGTTGCCTGTATTGCCATAACCCGGATACTTGGAACCCGAAAGGGAAGGTGAAATATCAGATGACTC
CCGGCGAGCTATTGACGGAAGTTTTACGGTATAAGAGTTTTATCGCCCGTGGGGGGGTGACTGTTACCGGTGGGGAACCT
TTATTGCAACCTGAGTTTCTGAAAGAGTTTTTCCGTTTGTGCCAAGAGCAAGGTCTTCACACAGCTTTGGACACATCTGG
TTTCGTATGTACCTCAAAAGCTTGGGAAGTCTTGGATTATGCGGACTTAGTGTTATTGGACATCAAAACCTTAAACCCGG
ATTTGCATCCGTTACTGGCGGGTGTCAAGCAGGATAATACGCTTCTGTTTTTGGATGAATTGGAGAGACGTGGCATAGAT
ACTTGGATTCGCCATGTTATTGTCCCCGGATATACGGATAATGACGAGTGGCTGGAGGCGTTAGCTCGGTATGTCAGTTC
ATATAAAGTGGTTCGCAAAGTGGAATTGTTGCCTTATCATACGATGGGAACCTATAAATATGAGCAATTAGGCCTCGATT
ATCCGTTAAAGGGGGTGGAGCCCTTATCAAAAGAACGTTTAGATAATGCGAAAGCGATATTCTCTCGCTACAAACCAGAA
AATAACAAGGCCTGA

Upstream 100 bases:

>100_bases
ACTTACGATCCGTGTATCCGGATATGCGGTAAACTTCGTGAAACTGACTCGTGAACAACAGTTGGATGTTATTTCAAGAA
CCATTAACCATTCACTGTAA

Downstream 100 bases:

>100_bases
TCTCTCGATCAAGCCTTGCGATTATGCAACAGCTAATCAGGGAAAACAATCTAATCCGATTAGCTTTTAAACGATAGTAA
TAATTTTTTGCCTAGACTAA

Product: pyruvate-formate lyase-activating enzyme

Products: NA

Alternate protein names: PFL-activating enzyme; Formate-C-acetyltransferase-activating enzyme [H]

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MIKGKIHSLESFGTVDGPGIRFVVFMQGCPLRCLYCHNPDTWNPKGKVKYQMTPGELLTEVLRYKSFIARGGVTVTGGEP
LLQPEFLKEFFRLCQEQGLHTALDTSGFVCTSKAWEVLDYADLVLLDIKTLNPDLHPLLAGVKQDNTLLFLDELERRGID
TWIRHVIVPGYTDNDEWLEALARYVSSYKVVRKVELLPYHTMGTYKYEQLGLDYPLKGVEPLSKERLDNAKAIFSRYKPE
NNKA

Sequences:

>Translated_244_residues
MIKGKIHSLESFGTVDGPGIRFVVFMQGCPLRCLYCHNPDTWNPKGKVKYQMTPGELLTEVLRYKSFIARGGVTVTGGEP
LLQPEFLKEFFRLCQEQGLHTALDTSGFVCTSKAWEVLDYADLVLLDIKTLNPDLHPLLAGVKQDNTLLFLDELERRGID
TWIRHVIVPGYTDNDEWLEALARYVSSYKVVRKVELLPYHTMGTYKYEQLGLDYPLKGVEPLSKERLDNAKAIFSRYKPE
NNKA
>Mature_244_residues
MIKGKIHSLESFGTVDGPGIRFVVFMQGCPLRCLYCHNPDTWNPKGKVKYQMTPGELLTEVLRYKSFIARGGVTVTGGEP
LLQPEFLKEFFRLCQEQGLHTALDTSGFVCTSKAWEVLDYADLVLLDIKTLNPDLHPLLAGVKQDNTLLFLDELERRGID
TWIRHVIVPGYTDNDEWLEALARYVSSYKVVRKVELLPYHTMGTYKYEQLGLDYPLKGVEPLSKERLDNAKAIFSRYKPE
NNKA

Specific function: Activation of pyruvate formate-lyase under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine [H]

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the organic radical-activating enzymes family [H]

Homologues:

Organism=Escherichia coli, GI1787130, Length=240, Percent_Identity=45, Blast_Score=226, Evalue=9e-61,
Organism=Escherichia coli, GI1790389, Length=263, Percent_Identity=25.8555133079848, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI226510931, Length=167, Percent_Identity=32.3353293413174, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012838
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =1.97.1.4 [H]

Molecular weight: Translated: 27845; Mature: 27845

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: PS01087 RADICAL_ACTIVATING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKGKIHSLESFGTVDGPGIRFVVFMQGCPLRCLYCHNPDTWNPKGKVKYQMTPGELLTE
CCCCCCCCHHHCCCCCCCCEEEEEEECCCCEEEEEECCCCCCCCCCCEEEEECHHHHHHH
VLRYKSFIARGGVTVTGGEPLLQPEFLKEFFRLCQEQGLHTALDTSGFVCTSKAWEVLDY
HHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHHCCCCEECCCCCCEECCHHHHHHHH
ADLVLLDIKTLNPDLHPLLAGVKQDNTLLFLDELERRGIDTWIRHVIVPGYTDNDEWLEA
HCEEEEEEECCCCCHHHHHHCCCCCCEEEEEEHHHHCCHHHHHHHHCCCCCCCCHHHHHH
LARYVSSYKVVRKVELLPYHTMGTYKYEQLGLDYPLKGVEPLSKERLDNAKAIFSRYKPE
HHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCC
NNKA
CCCC
>Mature Secondary Structure
MIKGKIHSLESFGTVDGPGIRFVVFMQGCPLRCLYCHNPDTWNPKGKVKYQMTPGELLTE
CCCCCCCCHHHCCCCCCCCEEEEEEECCCCEEEEEECCCCCCCCCCCEEEEECHHHHHHH
VLRYKSFIARGGVTVTGGEPLLQPEFLKEFFRLCQEQGLHTALDTSGFVCTSKAWEVLDY
HHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHHCCCCEECCCCCCEECCHHHHHHHH
ADLVLLDIKTLNPDLHPLLAGVKQDNTLLFLDELERRGIDTWIRHVIVPGYTDNDEWLEA
HCEEEEEEECCCCCHHHHHHCCCCCCEEEEEEHHHHCCHHHHHHHHCCCCCCCCHHHHHH
LARYVSSYKVVRKVELLPYHTMGTYKYEQLGLDYPLKGVEPLSKERLDNAKAIFSRYKPE
HHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCC
NNKA
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8636053 [H]