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The map label for this gene is pflB [H]

Identifier: 150009973

GI number: 150009973

Start: 4124661

End: 4126901

Strand: Direct

Name: pflB [H]

Synonym: BDI_3392

Alternate gene names: 150009973

Gene position: 4124661-4126901 (Clockwise)

Preceding gene: 150009963

Following gene: 150009974

Centisome position: 85.73

GC content: 47.61

Gene sequence:

>2241_bases
ATGAAATTTGAGAATGAGTACTGGGATCACTTTAAGGGTGAGACCTGGAAGAGAGAGATCAATGTAAGAGATTTTATCCA
GAGTAACTATACGCCTTATGAAGGGGATGATTCCTTTTTAGTCGCTTCTTCCGAGAAGACCCGTAAGGTCTGGAACAAGC
TGACCGAGATGTTTAAGGTCGAGAGAGAAAAGGGAGTTTATGACGCCGAGACAAAATTACCTCAAGGGATCGATGTATAT
GGTCCGGGCTATATTGATAAGGAGAATGAGGTTATCGTTGGGTTACAAACCGATGCTCCGCTGAAACGTGGTATCTTCCC
GAAGGGTGGTATCCGTATGGTCGAGAATAGCTTGGAAGCCTACGGTTATCATTTAGACCCGATGACGAAAGAGATCTTCA
CGAAGTACCGTAAGACGCATAACGAGGGCGTTTTCTCCGCATATACGGAAGAGATGGTTGCCGCACGTCGTTCGGCTATC
ATCACCGGCTTGCCCGATGCGTATGGTAGAGGACGTATTATCGGTGACTATCGTCGTGTCGCTTTATATGGTACGGCAAT
CTTGATCGAGGAGAAAAAGCGTTTCTTGAATCGTTTGGATATCCAAGAGATCACGGAAGAGATTATCCAGAGCCGTGAGG
AGATTTCTGAGCAAATCAAGGCGTTGAAAGCGTTCGAGAGAATGTGCGCTAGTTATGGCTTCGACGTGACCCGCCCGGCG
CAAAATGCTCGTGAGGCGGTTCAGTTTGTGTATTTGGCTTATCTGGCAGCGGTAAAAGATCAGGATGGTGCGGCAATGTC
TATCGGACGTACCTCTACATTCTTGGATATTTACATCGAGAAAGACATCCGTGAGGGTAAGATGACGGAAGAGGAGGCGC
AAGAGTTAGTAGACCAGCTGATCATCAAGTTGCGTATCGTTCGTTTCTTACGTACACCGGAATACAATGATTTGTTCTCC
GGTGATCCGGTTTGGGTAACAGAGTCGTTGGGCGGTCAAGGAGTGGACGGACGTTCTTTGGTAACGAGAACTTCCTATCG
TTATCTCCATACATTGTATAATTTAGGTCCGGCACCGGAACCCAACTTGACGGTGCTGTGGTTCAAGAACGCTCCCGAGA
ACTGGAAACGTTTCTGTGCCAAGGTCTCTATCGATACGTCGGCTATCCAATATGAGAATGACGATTTGATGCGTCCGGAT
TATGGGGATGATTATGGTATCGCTTGTTGCGTTTCCCCCATGAAGATCGGAAAGCAGATGCAGTTCTTCGGCGCTCGTGC
GAATTTAGCGAAGTGTTTATTGTATGCGATCAATGGAGGACGGGATGAGCGTAGCGGCGTACAGGTAGCCCCGATGTTCG
AACCGGTTCGCAGTGAGTATCTGGAGTATGATGAGGTGATGGCTAAGTATGAACAAATGATGCGCTGGTTGGCGAAGGTA
TATGTGAACGCCTTGAAGATAATCCATTATATGCATGATAAATATGCGTATGAGGCATTCGAGATGTCGCTTCACGATGG
GGATGTAGAGCGTATCCGTGCGACAGGTATCGCCGGTCTTTCTATCGTTGCCGATTCGTTGGCTGCTATCCGTGATACGA
AAGTACGTGTGATCCGTGACGAGCGTGGCTTGGCGGTTGATTTCGAACGTGAGGGCGAATATGTTCCTTTCGGTAATAAC
GATGATCGTACGGATAGTATCGCTGTAGAGATCACCGAGAAGTTCATGGAATATTTGCGTCAGCACGAGACCTATCGCCA
TGCGAAACCTACGCAGTCCATCCTTACGATCACCTCTAATGTGGTATATGGTAAGAAGACTGGTACGACACCGGATGGCC
GTATCGGTGGAACTCCTTTCGCTCCGGGTGCGAACCCTATGAACGGTCGTGATACGAAAGGTGCTATCGCTGCGCTAGCT
TCGGTAGCGAAGCTTCCATTCCAGCATGCGCACGACGGCATCTCTTATACTTTCGCTGTTTCTCCGGCTACGTTGGGAAA
GGAGCGTGAAATGCAGATCAATAATCTGGTTAGCTTATTGGATGGCTATTTTACTCCGGATGGAGGTCAGCATCTGAATG
TTAACGTATTTGATAAGGATTTGTTGATCGACGCTATGGAACATCCCGAGAAATATCCGCAACTTACGATCCGTGTATCC
GGATATGCGGTAAACTTCGTGAAACTGACTCGTGAACAACAGTTGGATGTTATTTCAAGAACCATTAACCATTCACTGTA
A

Upstream 100 bases:

>100_bases
TTATAGGGATTTTAGAGGGGTGTCGCAAATGTTACAGGAACATGAGGATAAAAGTTTTACCTTTGTGCCGGTAAAAATGT
GGTATTAGATAAGATTGTTT

Downstream 100 bases:

>100_bases
GTGATAAAAGGTAAGATACATTCATTAGAGAGTTTCGGGACCGTAGATGGTCCCGGAATTCGCTTTGTCGTTTTTATGCA
GGGCTGCCCGTTGCGTTGCC

Product: formate acetyltransferase

Products: NA

Alternate protein names: Pyruvate formate-lyase [H]

Number of amino acids: Translated: 746; Mature: 746

Protein sequence:

>746_residues
MKFENEYWDHFKGETWKREINVRDFIQSNYTPYEGDDSFLVASSEKTRKVWNKLTEMFKVEREKGVYDAETKLPQGIDVY
GPGYIDKENEVIVGLQTDAPLKRGIFPKGGIRMVENSLEAYGYHLDPMTKEIFTKYRKTHNEGVFSAYTEEMVAARRSAI
ITGLPDAYGRGRIIGDYRRVALYGTAILIEEKKRFLNRLDIQEITEEIIQSREEISEQIKALKAFERMCASYGFDVTRPA
QNAREAVQFVYLAYLAAVKDQDGAAMSIGRTSTFLDIYIEKDIREGKMTEEEAQELVDQLIIKLRIVRFLRTPEYNDLFS
GDPVWVTESLGGQGVDGRSLVTRTSYRYLHTLYNLGPAPEPNLTVLWFKNAPENWKRFCAKVSIDTSAIQYENDDLMRPD
YGDDYGIACCVSPMKIGKQMQFFGARANLAKCLLYAINGGRDERSGVQVAPMFEPVRSEYLEYDEVMAKYEQMMRWLAKV
YVNALKIIHYMHDKYAYEAFEMSLHDGDVERIRATGIAGLSIVADSLAAIRDTKVRVIRDERGLAVDFEREGEYVPFGNN
DDRTDSIAVEITEKFMEYLRQHETYRHAKPTQSILTITSNVVYGKKTGTTPDGRIGGTPFAPGANPMNGRDTKGAIAALA
SVAKLPFQHAHDGISYTFAVSPATLGKEREMQINNLVSLLDGYFTPDGGQHLNVNVFDKDLLIDAMEHPEKYPQLTIRVS
GYAVNFVKLTREQQLDVISRTINHSL

Sequences:

>Translated_746_residues
MKFENEYWDHFKGETWKREINVRDFIQSNYTPYEGDDSFLVASSEKTRKVWNKLTEMFKVEREKGVYDAETKLPQGIDVY
GPGYIDKENEVIVGLQTDAPLKRGIFPKGGIRMVENSLEAYGYHLDPMTKEIFTKYRKTHNEGVFSAYTEEMVAARRSAI
ITGLPDAYGRGRIIGDYRRVALYGTAILIEEKKRFLNRLDIQEITEEIIQSREEISEQIKALKAFERMCASYGFDVTRPA
QNAREAVQFVYLAYLAAVKDQDGAAMSIGRTSTFLDIYIEKDIREGKMTEEEAQELVDQLIIKLRIVRFLRTPEYNDLFS
GDPVWVTESLGGQGVDGRSLVTRTSYRYLHTLYNLGPAPEPNLTVLWFKNAPENWKRFCAKVSIDTSAIQYENDDLMRPD
YGDDYGIACCVSPMKIGKQMQFFGARANLAKCLLYAINGGRDERSGVQVAPMFEPVRSEYLEYDEVMAKYEQMMRWLAKV
YVNALKIIHYMHDKYAYEAFEMSLHDGDVERIRATGIAGLSIVADSLAAIRDTKVRVIRDERGLAVDFEREGEYVPFGNN
DDRTDSIAVEITEKFMEYLRQHETYRHAKPTQSILTITSNVVYGKKTGTTPDGRIGGTPFAPGANPMNGRDTKGAIAALA
SVAKLPFQHAHDGISYTFAVSPATLGKEREMQINNLVSLLDGYFTPDGGQHLNVNVFDKDLLIDAMEHPEKYPQLTIRVS
GYAVNFVKLTREQQLDVISRTINHSL
>Mature_746_residues
MKFENEYWDHFKGETWKREINVRDFIQSNYTPYEGDDSFLVASSEKTRKVWNKLTEMFKVEREKGVYDAETKLPQGIDVY
GPGYIDKENEVIVGLQTDAPLKRGIFPKGGIRMVENSLEAYGYHLDPMTKEIFTKYRKTHNEGVFSAYTEEMVAARRSAI
ITGLPDAYGRGRIIGDYRRVALYGTAILIEEKKRFLNRLDIQEITEEIIQSREEISEQIKALKAFERMCASYGFDVTRPA
QNAREAVQFVYLAYLAAVKDQDGAAMSIGRTSTFLDIYIEKDIREGKMTEEEAQELVDQLIIKLRIVRFLRTPEYNDLFS
GDPVWVTESLGGQGVDGRSLVTRTSYRYLHTLYNLGPAPEPNLTVLWFKNAPENWKRFCAKVSIDTSAIQYENDDLMRPD
YGDDYGIACCVSPMKIGKQMQFFGARANLAKCLLYAINGGRDERSGVQVAPMFEPVRSEYLEYDEVMAKYEQMMRWLAKV
YVNALKIIHYMHDKYAYEAFEMSLHDGDVERIRATGIAGLSIVADSLAAIRDTKVRVIRDERGLAVDFEREGEYVPFGNN
DDRTDSIAVEITEKFMEYLRQHETYRHAKPTQSILTITSNVVYGKKTGTTPDGRIGGTPFAPGANPMNGRDTKGAIAALA
SVAKLPFQHAHDGISYTFAVSPATLGKEREMQINNLVSLLDGYFTPDGGQHLNVNVFDKDLLIDAMEHPEKYPQLTIRVS
GYAVNFVKLTREQQLDVISRTINHSL

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1787131, Length=749, Percent_Identity=61.415220293725, Blast_Score=971, Evalue=0.0,
Organism=Escherichia coli, GI48994926, Length=755, Percent_Identity=59.8675496688742, Blast_Score=940, Evalue=0.0,
Organism=Escherichia coli, GI1787044, Length=611, Percent_Identity=25.8592471358429, Blast_Score=185, Evalue=8e-48,
Organism=Escherichia coli, GI1790388, Length=664, Percent_Identity=25.1506024096386, Blast_Score=139, Evalue=6e-34,
Organism=Escherichia coli, GI1788933, Length=60, Percent_Identity=70, Blast_Score=89, Evalue=1e-18,

Paralogues:

None

Copy number: 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005949
- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 84884; Mature: 84884

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFENEYWDHFKGETWKREINVRDFIQSNYTPYEGDDSFLVASSEKTRKVWNKLTEMFKV
CCCCCCHHHHCCCCCHHHCCCHHHHHHCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHH
EREKGVYDAETKLPQGIDVYGPGYIDKENEVIVGLQTDAPLKRGIFPKGGIRMVENSLEA
HHHCCCCCCHHCCCCCCEECCCCCCCCCCCEEEEEECCCCHHHCCCCCCCHHHHHHHHHH
YGYHLDPMTKEIFTKYRKTHNEGVFSAYTEEMVAARRSAIITGLPDAYGRGRIIGDYRRV
CCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHEEECCCCCCCCCCEEHHHHEE
ALYGTAILIEEKKRFLNRLDIQEITEEIIQSREEISEQIKALKAFERMCASYGFDVTRPA
EEHHHHEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
QNAREAVQFVYLAYLAAVKDQDGAAMSIGRTSTFLDIYIEKDIREGKMTEEEAQELVDQL
HHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEEECCCCCCCCCHHHHHHHHHHH
IIKLRIVRFLRTPEYNDLFSGDPVWVTESLGGQGVDGRSLVTRTSYRYLHTLYNLGPAPE
HHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCC
PNLTVLWFKNAPENWKRFCAKVSIDTSAIQYENDDLMRPDYGDDYGIACCVSPMKIGKQM
CCEEEEEECCCCHHHHHHHHHHEECCCEEEECCCCCCCCCCCCCCCEEEECCHHHHCCHH
QFFGARANLAKCLLYAINGGRDERSGVQVAPMFEPVRSEYLEYDEVMAKYEQMMRWLAKV
HHHCCHHHHHHHHHHHHCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YVNALKIIHYMHDKYAYEAFEMSLHDGDVERIRATGIAGLSIVADSLAAIRDTKVRVIRD
HHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHCCCCHHHHHHHHHHHHHCCEEEEEEC
ERGLAVDFEREGEYVPFGNNDDRTDSIAVEITEKFMEYLRQHETYRHAKPTQSILTITSN
CCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHC
VVYGKKTGTTPDGRIGGTPFAPGANPMNGRDTKGAIAALASVAKLPFQHAHDGISYTFAV
EEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHCCCCCEEEEEE
SPATLGKEREMQINNLVSLLDGYFTPDGGQHLNVNVFDKDLLIDAMEHPEKYPQLTIRVS
CCHHCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECHHHHHHHHHCCCCCCEEEEEEE
GYAVNFVKLTREQQLDVISRTINHSL
CEEEEEEHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKFENEYWDHFKGETWKREINVRDFIQSNYTPYEGDDSFLVASSEKTRKVWNKLTEMFKV
CCCCCCHHHHCCCCCHHHCCCHHHHHHCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHH
EREKGVYDAETKLPQGIDVYGPGYIDKENEVIVGLQTDAPLKRGIFPKGGIRMVENSLEA
HHHCCCCCCHHCCCCCCEECCCCCCCCCCCEEEEEECCCCHHHCCCCCCCHHHHHHHHHH
YGYHLDPMTKEIFTKYRKTHNEGVFSAYTEEMVAARRSAIITGLPDAYGRGRIIGDYRRV
CCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHEEECCCCCCCCCCEEHHHHEE
ALYGTAILIEEKKRFLNRLDIQEITEEIIQSREEISEQIKALKAFERMCASYGFDVTRPA
EEHHHHEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
QNAREAVQFVYLAYLAAVKDQDGAAMSIGRTSTFLDIYIEKDIREGKMTEEEAQELVDQL
HHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEEECCCCCCCCCHHHHHHHHHHH
IIKLRIVRFLRTPEYNDLFSGDPVWVTESLGGQGVDGRSLVTRTSYRYLHTLYNLGPAPE
HHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCC
PNLTVLWFKNAPENWKRFCAKVSIDTSAIQYENDDLMRPDYGDDYGIACCVSPMKIGKQM
CCEEEEEECCCCHHHHHHHHHHEECCCEEEECCCCCCCCCCCCCCCEEEECCHHHHCCHH
QFFGARANLAKCLLYAINGGRDERSGVQVAPMFEPVRSEYLEYDEVMAKYEQMMRWLAKV
HHHCCHHHHHHHHHHHHCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YVNALKIIHYMHDKYAYEAFEMSLHDGDVERIRATGIAGLSIVADSLAAIRDTKVRVIRD
HHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHCCCCHHHHHHHHHHHHHCCEEEEEEC
ERGLAVDFEREGEYVPFGNNDDRTDSIAVEITEKFMEYLRQHETYRHAKPTQSILTITSN
CCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHC
VVYGKKTGTTPDGRIGGTPFAPGANPMNGRDTKGAIAALASVAKLPFQHAHDGISYTFAV
EEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHCCCCCEEEEEE
SPATLGKEREMQINNLVSLLDGYFTPDGGQHLNVNVFDKDLLIDAMEHPEKYPQLTIRVS
CCHHCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECHHHHHHHHHCCCCCCEEEEEEE
GYAVNFVKLTREQQLDVISRTINHSL
CEEEEEEHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA