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The map label for this gene is lacZ [H]

Identifier: 150009880

GI number: 150009880

Start: 3985741

End: 3987981

Strand: Direct

Name: lacZ [H]

Synonym: BDI_3296

Alternate gene names: 150009880

Gene position: 3985741-3987981 (Clockwise)

Preceding gene: 150009879

Following gene: 150009881

Centisome position: 82.84

GC content: 44.67

Gene sequence:

>2241_bases
ATGAAAAGAATAATTTTAATTATGTGTTGCTTCCTATCCGTATTCGCTTGGGCCGATGACGGGAGGACAACGATCTCATT
AAATGGGGAATGGGATTTTGATCAGACGGAACATGCTTTTCCCCCACGGAAATATACCCGTAAGATACCGGTTCCGGGAT
TGGTGCATCTAGCTAGGCCGAAAATTTCTCAATACGAGAAATTCTTCAAGAAGCCTGACGGCGTGGAGTTGGTGGAACAG
TTTAATTTCTTGGAGAGAGATTATACGCCAATGTATAATTGGTATAAGCGAAAAGTATTTATCGACGAGAAATTCAAGGA
TGAGCAATTATTCCTTACGATAAAGAAAAGCCAATACGTTACACGAGTCTTCGTGAATGGACATCAAGTAGGGTCTTCCA
TGGAATGCTATACTCCAATGGATTTTAATATTACGAGTGCCGTAAAATATGGCTCGGACAATGAGATCTTAATTCAGGTG
GGCGACCGGGCGTGGTTGCCTAGCGAGGCTGCCGGTGGTACGGATAAGGAGAAAGTCCATTACCTACCCGGAATCTGGGA
CGACGTGTTCATCACGGCTACCGGTAAGATGCGGGTCGACAAGATTTTGTTCCTACCTTCTCTGGCTAAAGGATTGGTGA
CAGTTAAGACTTTGGTGAGAAGTTTGTATCCACCCCAAATGTTGTATGGGGATAAAATGAAGGACTCTTGTAAGATTGAG
TTCTGTGTCAAGGAGAAGACCACAGGGCAGGTGGTTGGAAAGAAAGTGATTGAAGGTGAGGTTAAACGGGATAATAGGAC
TTATTTCGAAACGAGTATCTCTTTGGATAATCCGAAGGCTTGGACTCCGGATTCCCCTTTCTTGTACGAAGGCGAGGTCT
CGGTGTATGACCAAGATGAGCTTGTAGATCGTTATTCGGTGAACTTCGGGATGCGTGATTTTTCGAGAAAAGGCAAATTC
TTTACGCTTAATGGGGATAAATTCTACTTACGAGGCTCCAATATCACGCTTCAACGTTTCTTTGAGGACCCGGATTGTCA
AGCGTTGGCTTGGGATCGGGAATGGGTGAAGAAGCTGATGATAGACTTGCCAAAGTCGATTGATTGGAACGCTATGCGAA
TATGTGTCGGTATTGTTCCGGACTTCTGGTATGATCTTTGCGATGAGTATGGCATCGTATTGCAAAATGAGTGGCTTTAT
TGGCAAAATCATGGATGGGATGAACAGGTACGTAAGGAGTATACGAATTGGGTATGGAGCGATGGCAATCATCCCAGTAT
CGTGATCTGGGATGCGATCAATGAGAATTGGGACAGTTATATAGGAAATACGCTGATTCCCGAACTAAAGGAATTAGACC
CGACCCGGATCTGGGATGCCGGCTATATGACATCTGATCAGATGGGAACGAATGATGAGATGGATGAGCCACACCCATAT
CGCGCGCTAACGTTGATGCATTCCAGTGAACTGAACGACTACTTCAAAAACAATCCCTATAACTTAGGTGCATTGCATGA
GAATTGGGTAGGTTTCTCTTCTATACTAGATGCGGGCGTTCCTCAGTTGGTCAATGAGTACGGATGGATTTGGTTATGGC
GTGATGGACGGCCCTCGAAATTGACACTCAATAATTATAATTATTATCTCGGCGAGAACGCTACTCCGGCTCAATGCCGT
GAATTGCAAGCCTATTGGTTACAGCTTGAGACCGAGTGGCTGCGTTCAGAACGCTCGGTGGGTGGAATCTTGGCCTTTTG
CCATCTGACCAATAATTATGGTTTCACGGGAGATTGGTTTATCAATGATATTAAGGACTTGCAACCTTCGCCGGCTTTCC
GGTGGTTCAAGCATTGCTTCGCTCCAACGGCGGTCTTTATCGACTTGACGGACCATCGTTATACAAAACATCTCCCTGCC
TTGAAACCGGGTAGTGATTTAGTCTTCAACTTGGTTGGGGTGAACGACTTGAATAAAGACTCGTCAGGAAAAGTTCTCTT
GAAACTACTAGACGAGAAAGGAACTATTATTTCGACACAAGAAGAGTCTATTGTGATTGAACCTTTTGGCAAGCGGCTTC
AACCTTGCCTTCTGAAATTGCCCTCAAAAGCAGGAGGTTATCTTTTGATAGCCGAGTATCATGAGAAGGGGGGGGCGAAA
CCAGTACTTAGTCGCCGTTATCTAAAAGTAGGCGATGCGGTAACCTCTTTCAAGGATTACTTTGAGTATACATTAAATTA
G

Upstream 100 bases:

>100_bases
TTTTGATAAGATTCTAAAAGCAGGAGGTAAATTTCTAAAAAGTTTATGTTTCTAATAATTGTAACTTGCCTTCGTGAAAG
AGTTAAATAAATCGGATGTC

Downstream 100 bases:

>100_bases
TTAGGTTAGCTTTTATATCCAATTTCCTACGGCGGCACTCCTCGTGAGTGCTGTCGAGGGGAATGCTACCCCTTTTTTGA
AAATACTCAGAGTCCGAATA

Product: beta-glycosidase

Products: NA

Alternate protein names: Beta-gal; Lactase [H]

Number of amino acids: Translated: 746; Mature: 746

Protein sequence:

>746_residues
MKRIILIMCCFLSVFAWADDGRTTISLNGEWDFDQTEHAFPPRKYTRKIPVPGLVHLARPKISQYEKFFKKPDGVELVEQ
FNFLERDYTPMYNWYKRKVFIDEKFKDEQLFLTIKKSQYVTRVFVNGHQVGSSMECYTPMDFNITSAVKYGSDNEILIQV
GDRAWLPSEAAGGTDKEKVHYLPGIWDDVFITATGKMRVDKILFLPSLAKGLVTVKTLVRSLYPPQMLYGDKMKDSCKIE
FCVKEKTTGQVVGKKVIEGEVKRDNRTYFETSISLDNPKAWTPDSPFLYEGEVSVYDQDELVDRYSVNFGMRDFSRKGKF
FTLNGDKFYLRGSNITLQRFFEDPDCQALAWDREWVKKLMIDLPKSIDWNAMRICVGIVPDFWYDLCDEYGIVLQNEWLY
WQNHGWDEQVRKEYTNWVWSDGNHPSIVIWDAINENWDSYIGNTLIPELKELDPTRIWDAGYMTSDQMGTNDEMDEPHPY
RALTLMHSSELNDYFKNNPYNLGALHENWVGFSSILDAGVPQLVNEYGWIWLWRDGRPSKLTLNNYNYYLGENATPAQCR
ELQAYWLQLETEWLRSERSVGGILAFCHLTNNYGFTGDWFINDIKDLQPSPAFRWFKHCFAPTAVFIDLTDHRYTKHLPA
LKPGSDLVFNLVGVNDLNKDSSGKVLLKLLDEKGTIISTQEESIVIEPFGKRLQPCLLKLPSKAGGYLLIAEYHEKGGAK
PVLSRRYLKVGDAVTSFKDYFEYTLN

Sequences:

>Translated_746_residues
MKRIILIMCCFLSVFAWADDGRTTISLNGEWDFDQTEHAFPPRKYTRKIPVPGLVHLARPKISQYEKFFKKPDGVELVEQ
FNFLERDYTPMYNWYKRKVFIDEKFKDEQLFLTIKKSQYVTRVFVNGHQVGSSMECYTPMDFNITSAVKYGSDNEILIQV
GDRAWLPSEAAGGTDKEKVHYLPGIWDDVFITATGKMRVDKILFLPSLAKGLVTVKTLVRSLYPPQMLYGDKMKDSCKIE
FCVKEKTTGQVVGKKVIEGEVKRDNRTYFETSISLDNPKAWTPDSPFLYEGEVSVYDQDELVDRYSVNFGMRDFSRKGKF
FTLNGDKFYLRGSNITLQRFFEDPDCQALAWDREWVKKLMIDLPKSIDWNAMRICVGIVPDFWYDLCDEYGIVLQNEWLY
WQNHGWDEQVRKEYTNWVWSDGNHPSIVIWDAINENWDSYIGNTLIPELKELDPTRIWDAGYMTSDQMGTNDEMDEPHPY
RALTLMHSSELNDYFKNNPYNLGALHENWVGFSSILDAGVPQLVNEYGWIWLWRDGRPSKLTLNNYNYYLGENATPAQCR
ELQAYWLQLETEWLRSERSVGGILAFCHLTNNYGFTGDWFINDIKDLQPSPAFRWFKHCFAPTAVFIDLTDHRYTKHLPA
LKPGSDLVFNLVGVNDLNKDSSGKVLLKLLDEKGTIISTQEESIVIEPFGKRLQPCLLKLPSKAGGYLLIAEYHEKGGAK
PVLSRRYLKVGDAVTSFKDYFEYTLN
>Mature_746_residues
MKRIILIMCCFLSVFAWADDGRTTISLNGEWDFDQTEHAFPPRKYTRKIPVPGLVHLARPKISQYEKFFKKPDGVELVEQ
FNFLERDYTPMYNWYKRKVFIDEKFKDEQLFLTIKKSQYVTRVFVNGHQVGSSMECYTPMDFNITSAVKYGSDNEILIQV
GDRAWLPSEAAGGTDKEKVHYLPGIWDDVFITATGKMRVDKILFLPSLAKGLVTVKTLVRSLYPPQMLYGDKMKDSCKIE
FCVKEKTTGQVVGKKVIEGEVKRDNRTYFETSISLDNPKAWTPDSPFLYEGEVSVYDQDELVDRYSVNFGMRDFSRKGKF
FTLNGDKFYLRGSNITLQRFFEDPDCQALAWDREWVKKLMIDLPKSIDWNAMRICVGIVPDFWYDLCDEYGIVLQNEWLY
WQNHGWDEQVRKEYTNWVWSDGNHPSIVIWDAINENWDSYIGNTLIPELKELDPTRIWDAGYMTSDQMGTNDEMDEPHPY
RALTLMHSSELNDYFKNNPYNLGALHENWVGFSSILDAGVPQLVNEYGWIWLWRDGRPSKLTLNNYNYYLGENATPAQCR
ELQAYWLQLETEWLRSERSVGGILAFCHLTNNYGFTGDWFINDIKDLQPSPAFRWFKHCFAPTAVFIDLTDHRYTKHLPA
LKPGSDLVFNLVGVNDLNKDSSGKVLLKLLDEKGTIISTQEESIVIEPFGKRLQPCLLKLPSKAGGYLLIAEYHEKGGAK
PVLSRRYLKVGDAVTSFKDYFEYTLN

Specific function: Unknown

COG id: COG3250

COG function: function code G; Beta-galactosidase/beta-glucuronidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1786539, Length=387, Percent_Identity=21.7054263565891, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI48994920, Length=457, Percent_Identity=21.2253829321663, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003344
- InterPro:   IPR008979
- InterPro:   IPR006101
- InterPro:   IPR013812
- InterPro:   IPR006104
- InterPro:   IPR006102
- InterPro:   IPR006103
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00703 Glyco_hydro_2; PF02836 Glyco_hydro_2_C; PF02837 Glyco_hydro_2_N [H]

EC number: =3.2.1.23 [H]

Molecular weight: Translated: 86630; Mature: 86630

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRIILIMCCFLSVFAWADDGRTTISLNGEWDFDQTEHAFPPRKYTRKIPVPGLVHLARP
CCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCHHHCCCCCCCCHHHHCCC
KISQYEKFFKKPDGVELVEQFNFLERDYTPMYNWYKRKVFIDEKFKDEQLFLTIKKSQYV
CHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHEEEEEEECCCCCCCEEEEEEECCCEE
TRVFVNGHQVGSSMECYTPMDFNITSAVKYGSDNEILIQVGDRAWLPSEAAGGTDKEKVH
EEEEEECHHCCCCCEECCCCCCCHHHHEEECCCCEEEEEECCCCCCCCCCCCCCCCCEEE
YLPGIWDDVFITATGKMRVDKILFLPSLAKGLVTVKTLVRSLYPPQMLYGDKMKDSCKIE
ECCCCCCCEEEEEECCEEEEHEEECHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCEEE
FCVKEKTTGQVVGKKVIEGEVKRDNRTYFETSISLDNPKAWTPDSPFLYEGEVSVYDQDE
EEECCCCCHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCCCCEEEECEEEEECHHH
LVDRYSVNFGMRDFSRKGKFFTLNGDKFYLRGSNITLQRFFEDPDCQALAWDREWVKKLM
HHHHHCCCCCCHHHHCCCCEEEECCCEEEEECCCEEEEEECCCCCCCEEECCHHHHHHHH
IDLPKSIDWNAMRICVGIVPDFWYDLCDEYGIVLQNEWLYWQNHGWDEQVRKEYTNWVWS
HHCCCCCCCCCEEEEEHHCHHHHHHHHHCCCEEEECCEEEECCCCCHHHHHHHHCCCEEC
DGNHPSIVIWDAINENWDSYIGNTLIPELKELDPTRIWDAGYMTSDQMGTNDEMDEPHPY
CCCCCEEEEECCCCCCHHHHCCCCCCCHHHHCCCCEEECCCCCCCCCCCCCCCCCCCCCC
RALTLMHSSELNDYFKNNPYNLGALHENWVGFSSILDAGVPQLVNEYGWIWLWRDGRPSK
EEEEEEEHHHHHHHHCCCCEEECCCCCCCCCHHHHHHCCHHHHHHCCCEEEEEECCCCCE
LTLNNYNYYLGENATPAQCRELQAYWLQLETEWLRSERSVGGILAFCHLTNNYGFTGDWF
EEEECCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCHH
INDIKDLQPSPAFRWFKHCFAPTAVFIDLTDHRYTKHLPALKPGSDLVFNLVGVNDLNKD
HHHHHHCCCCHHHHHHHHHCCCEEEEEEECCCCHHHCCCCCCCCCCEEEEEECCCCCCCC
SSGKVLLKLLDEKGTIISTQEESIVIEPFGKRLQPCLLKLPSKAGGYLLIAEYHEKGGAK
CCCCEEEEEECCCCCEEECCCCEEEECCCCCHHHHHHHCCCCCCCCEEEEEEECCCCCCC
PVLSRRYLKVGDAVTSFKDYFEYTLN
CHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKRIILIMCCFLSVFAWADDGRTTISLNGEWDFDQTEHAFPPRKYTRKIPVPGLVHLARP
CCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCHHHCCCCCCCCHHHHCCC
KISQYEKFFKKPDGVELVEQFNFLERDYTPMYNWYKRKVFIDEKFKDEQLFLTIKKSQYV
CHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHEEEEEEECCCCCCCEEEEEEECCCEE
TRVFVNGHQVGSSMECYTPMDFNITSAVKYGSDNEILIQVGDRAWLPSEAAGGTDKEKVH
EEEEEECHHCCCCCEECCCCCCCHHHHEEECCCCEEEEEECCCCCCCCCCCCCCCCCEEE
YLPGIWDDVFITATGKMRVDKILFLPSLAKGLVTVKTLVRSLYPPQMLYGDKMKDSCKIE
ECCCCCCCEEEEEECCEEEEHEEECHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCEEE
FCVKEKTTGQVVGKKVIEGEVKRDNRTYFETSISLDNPKAWTPDSPFLYEGEVSVYDQDE
EEECCCCCHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCCCCEEEECEEEEECHHH
LVDRYSVNFGMRDFSRKGKFFTLNGDKFYLRGSNITLQRFFEDPDCQALAWDREWVKKLM
HHHHHCCCCCCHHHHCCCCEEEECCCEEEEECCCEEEEEECCCCCCCEEECCHHHHHHHH
IDLPKSIDWNAMRICVGIVPDFWYDLCDEYGIVLQNEWLYWQNHGWDEQVRKEYTNWVWS
HHCCCCCCCCCEEEEEHHCHHHHHHHHHCCCEEEECCEEEECCCCCHHHHHHHHCCCEEC
DGNHPSIVIWDAINENWDSYIGNTLIPELKELDPTRIWDAGYMTSDQMGTNDEMDEPHPY
CCCCCEEEEECCCCCCHHHHCCCCCCCHHHHCCCCEEECCCCCCCCCCCCCCCCCCCCCC
RALTLMHSSELNDYFKNNPYNLGALHENWVGFSSILDAGVPQLVNEYGWIWLWRDGRPSK
EEEEEEEHHHHHHHHCCCCEEECCCCCCCCCHHHHHHCCHHHHHHCCCEEEEEECCCCCE
LTLNNYNYYLGENATPAQCRELQAYWLQLETEWLRSERSVGGILAFCHLTNNYGFTGDWF
EEEECCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCHH
INDIKDLQPSPAFRWFKHCFAPTAVFIDLTDHRYTKHLPALKPGSDLVFNLVGVNDLNKD
HHHHHHCCCCHHHHHHHHHCCCEEEEEEECCCCHHHCCCCCCCCCCEEEEEECCCCCCCC
SSGKVLLKLLDEKGTIISTQEESIVIEPFGKRLQPCLLKLPSKAGGYLLIAEYHEKGGAK
CCCCEEEEEECCCCCEEECCCCEEEECCCCCHHHHHHHCCCCCCCCEEEEEEECCCCCCC
PVLSRRYLKVGDAVTSFKDYFEYTLN
CHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA