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The map label for this gene is nadB [H]

Identifier: 150009620

GI number: 150009620

Start: 3631361

End: 3632932

Strand: Reverse

Name: nadB [H]

Synonym: BDI_3033

Alternate gene names: 150009620

Gene position: 3632932-3631361 (Counterclockwise)

Preceding gene: 150009621

Following gene: 150009618

Centisome position: 75.51

GC content: 48.6

Gene sequence:

>1572_bases
ATGGTACAAAGATTCGATTACTTAGTGATAGGCTCAGGAATCGCCGGTATGAGTTTCGCTCTGAAAGTAGCCAATGAAGG
CAAGAGCGTAGCACTTATCTGTAAAGCCGGACAAGAAGAAGCCAACACATATTTCGCGCAAGGAGGTATCGCTTCCGTAA
CCAACTTGAAAGTAGATAATTTCGAGAAGCACATAGAAGACACGATGATCGCCGGCGACTGGATCAGTGACCGGGCGGCC
GTGGAGAAAGTCGTGCGCGAGGCCCCTGCCCAGATCGAGGCGCTTATCCGATGGGGAGTTAATTTCGATAAGAAAGAAAG
CGGAGAATTCGATCTGCATAAAGAAGGAGGACACTCCGAGTTTCGTATCCTGCACCATGCCGACAATACGGGAGCCGAGA
TACAGACGAGCTTGATAGAAACGGTAAACAACCATCCCAACATCGCCGTATTCACGAACCATTATGCGGTAGAGATTATC
ACCCAGCACCATATGGGCATTATCGTCACCCGCCATACCCCCGGTATCAAATGTTTCGGTGCCTATGTATTGAACGAGGA
TACGGGCGAGGTACATACTTTCTTGTCAAAGATAACCGTTATGGCGACAGGCGGTTGCGAGGCCGTTTACCGGAATACGA
CAAACCCGCTGGTAGCTACCGGAGACGGTATCGCCATGGTTTACCGCGCGAAAGGCTCCGTAAAAGATATGGAGTTTATC
CAGTTCCACCCGACCGCCTTGTATCATCCGGGCGACCGTCCCTCTTTCCTCATCACCGAGGCGATGCGCGGATATGGAGC
TGTATTGAGAAACCTTGGCGGCGAGGAGTTCATGCATAAATATGATCCCCGTCTTTCCTTGGCTCCCCGTGATATCGTAG
CCCGTGCGATCGATAGCGAGATGAAACAACGGGGTGAAGACCATGTCTATTTGGACGTGACGCACAAAGATCCGGAAGAG
ACGAAGAAACACTTCCCCAATATCTATAAGAAATGCCTCAGCTTAGGGATCGATATCACGAAAGATTACATCCCGGTAGC
CCCTGCGGCCCATTATTTATGTGGAGGTATCAAGGTAGACTTAAACGGGCAATCCAGCATCCGTCGCCTATATGCGATCG
GAGAATGTTCTTGTACCGGCCTGCACGGTGGTAACCGCTTAGCTTCAAACTCATTGATCGAGGCAGTTGTATATGCCGAC
GCCGCGGCAAAACACTCATTAAGCGTCATGGATCGCTATGAGTTTAACCAAGATGTACCGGAATGGAACGATGAGGGAAC
GATCACCAATGAAGAGCGTGTATTGATCACTCAAAGCATGAAAGAGGTAAACCAGATCATGGAAGCGTATGTAGGTATCG
TTCGTAGTAACACACGTTTGATCCGTGCGTGGAACCGTTTGGATATTTTATATGAGGAAACAGAGAGCCTTTTCAAACGT
TGTAAGGCATCCCGTGAATTATGTGAGTTGCGTAATATGATCAATGTAGGTTATTTGATCACTCGTCAAGCGATGGAACG
GAAAGAGAGCCGGGGATTACATTATACGATTGATTACCCACCGATTAAATAA

Upstream 100 bases:

>100_bases
GGAAACCTGTAGTCAATATTTATTTTGTACGAAAAGCAAGCGCAAAAGGAACAACCTAATCAGTTAATCCCTAACTTTGT
GAGCCTAAACAGTAGAAAAC

Downstream 100 bases:

>100_bases
TTGAAAATTGAGAATTGAGAATTACTAGCTATGTTAATTCTCAATTCTCAACTTTTAATTATCAATAGTTCTGTTTCATC
GGTTCAAAGTAAGCTTGCGG

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 523; Mature: 523

Protein sequence:

>523_residues
MVQRFDYLVIGSGIAGMSFALKVANEGKSVALICKAGQEEANTYFAQGGIASVTNLKVDNFEKHIEDTMIAGDWISDRAA
VEKVVREAPAQIEALIRWGVNFDKKESGEFDLHKEGGHSEFRILHHADNTGAEIQTSLIETVNNHPNIAVFTNHYAVEII
TQHHMGIIVTRHTPGIKCFGAYVLNEDTGEVHTFLSKITVMATGGCEAVYRNTTNPLVATGDGIAMVYRAKGSVKDMEFI
QFHPTALYHPGDRPSFLITEAMRGYGAVLRNLGGEEFMHKYDPRLSLAPRDIVARAIDSEMKQRGEDHVYLDVTHKDPEE
TKKHFPNIYKKCLSLGIDITKDYIPVAPAAHYLCGGIKVDLNGQSSIRRLYAIGECSCTGLHGGNRLASNSLIEAVVYAD
AAAKHSLSVMDRYEFNQDVPEWNDEGTITNEERVLITQSMKEVNQIMEAYVGIVRSNTRLIRAWNRLDILYEETESLFKR
CKASRELCELRNMINVGYLITRQAMERKESRGLHYTIDYPPIK

Sequences:

>Translated_523_residues
MVQRFDYLVIGSGIAGMSFALKVANEGKSVALICKAGQEEANTYFAQGGIASVTNLKVDNFEKHIEDTMIAGDWISDRAA
VEKVVREAPAQIEALIRWGVNFDKKESGEFDLHKEGGHSEFRILHHADNTGAEIQTSLIETVNNHPNIAVFTNHYAVEII
TQHHMGIIVTRHTPGIKCFGAYVLNEDTGEVHTFLSKITVMATGGCEAVYRNTTNPLVATGDGIAMVYRAKGSVKDMEFI
QFHPTALYHPGDRPSFLITEAMRGYGAVLRNLGGEEFMHKYDPRLSLAPRDIVARAIDSEMKQRGEDHVYLDVTHKDPEE
TKKHFPNIYKKCLSLGIDITKDYIPVAPAAHYLCGGIKVDLNGQSSIRRLYAIGECSCTGLHGGNRLASNSLIEAVVYAD
AAAKHSLSVMDRYEFNQDVPEWNDEGTITNEERVLITQSMKEVNQIMEAYVGIVRSNTRLIRAWNRLDILYEETESLFKR
CKASRELCELRNMINVGYLITRQAMERKESRGLHYTIDYPPIK
>Mature_523_residues
MVQRFDYLVIGSGIAGMSFALKVANEGKSVALICKAGQEEANTYFAQGGIASVTNLKVDNFEKHIEDTMIAGDWISDRAA
VEKVVREAPAQIEALIRWGVNFDKKESGEFDLHKEGGHSEFRILHHADNTGAEIQTSLIETVNNHPNIAVFTNHYAVEII
TQHHMGIIVTRHTPGIKCFGAYVLNEDTGEVHTFLSKITVMATGGCEAVYRNTTNPLVATGDGIAMVYRAKGSVKDMEFI
QFHPTALYHPGDRPSFLITEAMRGYGAVLRNLGGEEFMHKYDPRLSLAPRDIVARAIDSEMKQRGEDHVYLDVTHKDPEE
TKKHFPNIYKKCLSLGIDITKDYIPVAPAAHYLCGGIKVDLNGQSSIRRLYAIGECSCTGLHGGNRLASNSLIEAVVYAD
AAAKHSLSVMDRYEFNQDVPEWNDEGTITNEERVLITQSMKEVNQIMEAYVGIVRSNTRLIRAWNRLDILYEETESLFKR
CKASRELCELRNMINVGYLITRQAMERKESRGLHYTIDYPPIK

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=541, Percent_Identity=31.4232902033272, Blast_Score=230, Evalue=2e-60,
Organism=Escherichia coli, GI1788928, Length=524, Percent_Identity=47.1374045801527, Blast_Score=459, Evalue=1e-130,
Organism=Escherichia coli, GI1786942, Length=572, Percent_Identity=31.1188811188811, Blast_Score=229, Evalue=2e-61,
Organism=Escherichia coli, GI1790597, Length=529, Percent_Identity=31.0018903591682, Blast_Score=227, Evalue=2e-60,
Organism=Caenorhabditis elegans, GI17505833, Length=538, Percent_Identity=31.2267657992565, Blast_Score=229, Evalue=2e-60,
Organism=Caenorhabditis elegans, GI17550100, Length=537, Percent_Identity=30.5400372439479, Blast_Score=221, Evalue=5e-58,
Organism=Caenorhabditis elegans, GI71986328, Length=477, Percent_Identity=27.4633123689727, Blast_Score=96, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6322701, Length=556, Percent_Identity=32.1942446043165, Blast_Score=244, Evalue=2e-65,
Organism=Saccharomyces cerevisiae, GI6322416, Length=556, Percent_Identity=31.6546762589928, Blast_Score=234, Evalue=3e-62,
Organism=Saccharomyces cerevisiae, GI6320788, Length=484, Percent_Identity=27.0661157024793, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24663005, Length=566, Percent_Identity=31.9787985865724, Blast_Score=229, Evalue=5e-60,
Organism=Drosophila melanogaster, GI17137288, Length=558, Percent_Identity=30.6451612903226, Blast_Score=226, Evalue=4e-59,
Organism=Drosophila melanogaster, GI24655642, Length=558, Percent_Identity=30.6451612903226, Blast_Score=226, Evalue=4e-59,
Organism=Drosophila melanogaster, GI24655647, Length=558, Percent_Identity=30.6451612903226, Blast_Score=226, Evalue=4e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 58561; Mature: 58561

Theoretical pI: Translated: 6.47; Mature: 6.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQRFDYLVIGSGIAGMSFALKVANEGKSVALICKAGQEEANTYFAQGGIASVTNLKVDN
CCCEEEEEEEECCCCCCEEEEEEECCCCEEEEEEECCCCCCCEEEECCCCCEEEECCHHH
FEKHIEDTMIAGDWISDRAAVEKVVREAPAQIEALIRWGVNFDKKESGEFDLHKEGGHSE
HHHHHHHHEEECCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCEEEECCCCCCE
FRILHHADNTGAEIQTSLIETVNNHPNIAVFTNHYAVEIITQHHMGIIVTRHTPGIKCFG
EEEEEECCCCCHHHHHHHHHHHCCCCCEEEEECCEEEEEEEECCCEEEEEECCCCCEEEE
AYVLNEDTGEVHTFLSKITVMATGGCEAVYRNTTNPLVATGDGIAMVYRAKGSVKDMEFI
EEEEECCCHHHHHHHHHHEEEECCCCHHEEECCCCCEEEECCCEEEEEECCCCCCCCEEE
QFHPTALYHPGDRPSFLITEAMRGYGAVLRNLGGEEFMHKYDPRLSLAPRDIVARAIDSE
EECCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCHHHHHHHHHHHH
MKQRGEDHVYLDVTHKDPEETKKHFPNIYKKCLSLGIDITKDYIPVAPAAHYLCGGIKVD
HHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCCEEEE
LNGQSSIRRLYAIGECSCTGLHGGNRLASNSLIEAVVYADAAAKHSLSVMDRYEFNQDVP
ECCHHHHHHHHEECCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
EWNDEGTITNEERVLITQSMKEVNQIMEAYVGIVRSNTRLIRAWNRLDILYEETESLFKR
CCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHH
CKASRELCELRNMINVGYLITRQAMERKESRGLHYTIDYPPIK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCC
>Mature Secondary Structure
MVQRFDYLVIGSGIAGMSFALKVANEGKSVALICKAGQEEANTYFAQGGIASVTNLKVDN
CCCEEEEEEEECCCCCCEEEEEEECCCCEEEEEEECCCCCCCEEEECCCCCEEEECCHHH
FEKHIEDTMIAGDWISDRAAVEKVVREAPAQIEALIRWGVNFDKKESGEFDLHKEGGHSE
HHHHHHHHEEECCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCEEEECCCCCCE
FRILHHADNTGAEIQTSLIETVNNHPNIAVFTNHYAVEIITQHHMGIIVTRHTPGIKCFG
EEEEEECCCCCHHHHHHHHHHHCCCCCEEEEECCEEEEEEEECCCEEEEEECCCCCEEEE
AYVLNEDTGEVHTFLSKITVMATGGCEAVYRNTTNPLVATGDGIAMVYRAKGSVKDMEFI
EEEEECCCHHHHHHHHHHEEEECCCCHHEEECCCCCEEEECCCEEEEEECCCCCCCCEEE
QFHPTALYHPGDRPSFLITEAMRGYGAVLRNLGGEEFMHKYDPRLSLAPRDIVARAIDSE
EECCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCHHHHHHHHHHHH
MKQRGEDHVYLDVTHKDPEETKKHFPNIYKKCLSLGIDITKDYIPVAPAAHYLCGGIKVD
HHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCCEEEE
LNGQSSIRRLYAIGECSCTGLHGGNRLASNSLIEAVVYADAAAKHSLSVMDRYEFNQDVP
ECCHHHHHHHHEECCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
EWNDEGTITNEERVLITQSMKEVNQIMEAYVGIVRSNTRLIRAWNRLDILYEETESLFKR
CCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHH
CKASRELCELRNMINVGYLITRQAMERKESRGLHYTIDYPPIK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7737518; 10984043; 7961421 [H]