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The map label for this gene is bepIM [H]

Identifier: 150009588

GI number: 150009588

Start: 3590573

End: 3591850

Strand: Reverse

Name: bepIM [H]

Synonym: BDI_3001

Alternate gene names: 150009588

Gene position: 3591850-3590573 (Counterclockwise)

Preceding gene: 150009589

Following gene: 150009587

Centisome position: 74.65

GC content: 44.29

Gene sequence:

>1278_bases
ATGAAAATGAGTGAAAAGGAGGTTGATACATCAAATGAACTACGGGTCTTGTCCCTGTTCTCTGGATGTGGGGGCATGGA
TTTAGGATTCGAGGGGGATTTTATATGTCATCGTAAGTCTATATCTCCTCAAAGTACCTGGGTTCAAGAGGTTATCGATG
AACACTGGGTTCGTTTACAGAAGAATCGTTTTCGAACGGTCTTTGCGAATGATATATTGAAAGAGGCACAAACCGCTTGG
CTTCAATATATGGATCGATTCGGATATGATAAAAACGTTTATAATACAAAGAGTATCGTAGACTTGGTGAAAGAGCATCG
AGCGGGGAAAAAAGTTTTCCCTAAAAATATAGATGTCGTCACGGGAGGTTTTCCTTGCCAGGATTTCAGTGTCTCTGGTA
AACGTCTGGGATTTGAGTCGAATAAGGATCATACCGGGAAATTGCGTGAGGACGATGAGGCCTCGGAAGAGACGAGGGGT
AAACTTTACTATTGGATGAAGCAGGTGATCGATATTACGAGACCCAAGATATTTATCGCGGAGAACGTGAAGGGACTGGT
GAATCTGGGAGACGTGAAAAGTATTATTCAGAGAGACTTCGCCCATGCGGCTGGGGATGGATATATTGTTTTAGATCCTC
AAGTTTTACATGCGGGCAATTATGGGGTTCCGGAGTCTAGAGAGCGTGTGATTTTTATCGGGATACGTAAGTCCGCGTTA
AAGGTTAAGGCTTTAAGAGCGCTTCAAGAGGAGAAGATACCCAAAGCTTACGATCCTTATCCGAGTCCGACACATGCTTG
CACCGTGGAAGATAAGAAACTATTGCCACCGGTGGTTTGTCGGGATGTATTTGTAGGATTGGAGGAACCGGATAGGACAG
AAGATCCATCCCAGCGGTATTATTCACAGGCGAAATATATGGGAGCTCATTGTCAAGGTCAAAAGGAGATCGCGCTGGAT
AATATTGGCCCGACGATTCGTTCCGAGCATCATGGAAATATAGAATACCGTCGTTTATCCCGGGAGCATGGGGGCGTTAT
TACGGAAGAATTAGACGCCGGTTTACAAGAACGTCGCTTAACTCCTCGAGAATGTGCTTTGATACAGACGTTTCCGCCGG
ATTATACGTTTGTCTCCCATAAAAAGAACAGTACACGTTTTGAGTTAAGTGCCTCAGGCGCTTATAAGGTGATCGGTAAC
GCGGTTCCTCCAGTATTAGCGTATCATATAGCGAATCGCATACAGACTTTATGGCCGTTGTATTTTAAAAAGAGATAA

Upstream 100 bases:

>100_bases
GTGGATGCCGAATCAAAATCAATTGACTATGAAAACAAAAAAATTGAAATTCTTCGTAAATTTGTGGTTCTTTAAGCATA
TCTTATAGTAAGGATCGAAT

Downstream 100 bases:

>100_bases
TATGGAAGAGGAGAATATTATTACTGTGCGTAATAGAAATCTTCAAAAAGCGGATGAGGCTTTTGCGGATTTTATGTTTA
TGCTGGAATCATACTTGAAT

Product: site-specific DNA methylase

Products: NA

Alternate protein names: M.BepI; Cytosine-specific methyltransferase BepI [H]

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MKMSEKEVDTSNELRVLSLFSGCGGMDLGFEGDFICHRKSISPQSTWVQEVIDEHWVRLQKNRFRTVFANDILKEAQTAW
LQYMDRFGYDKNVYNTKSIVDLVKEHRAGKKVFPKNIDVVTGGFPCQDFSVSGKRLGFESNKDHTGKLREDDEASEETRG
KLYYWMKQVIDITRPKIFIAENVKGLVNLGDVKSIIQRDFAHAAGDGYIVLDPQVLHAGNYGVPESRERVIFIGIRKSAL
KVKALRALQEEKIPKAYDPYPSPTHACTVEDKKLLPPVVCRDVFVGLEEPDRTEDPSQRYYSQAKYMGAHCQGQKEIALD
NIGPTIRSEHHGNIEYRRLSREHGGVITEELDAGLQERRLTPRECALIQTFPPDYTFVSHKKNSTRFELSASGAYKVIGN
AVPPVLAYHIANRIQTLWPLYFKKR

Sequences:

>Translated_425_residues
MKMSEKEVDTSNELRVLSLFSGCGGMDLGFEGDFICHRKSISPQSTWVQEVIDEHWVRLQKNRFRTVFANDILKEAQTAW
LQYMDRFGYDKNVYNTKSIVDLVKEHRAGKKVFPKNIDVVTGGFPCQDFSVSGKRLGFESNKDHTGKLREDDEASEETRG
KLYYWMKQVIDITRPKIFIAENVKGLVNLGDVKSIIQRDFAHAAGDGYIVLDPQVLHAGNYGVPESRERVIFIGIRKSAL
KVKALRALQEEKIPKAYDPYPSPTHACTVEDKKLLPPVVCRDVFVGLEEPDRTEDPSQRYYSQAKYMGAHCQGQKEIALD
NIGPTIRSEHHGNIEYRRLSREHGGVITEELDAGLQERRLTPRECALIQTFPPDYTFVSHKKNSTRFELSASGAYKVIGN
AVPPVLAYHIANRIQTLWPLYFKKR
>Mature_425_residues
MKMSEKEVDTSNELRVLSLFSGCGGMDLGFEGDFICHRKSISPQSTWVQEVIDEHWVRLQKNRFRTVFANDILKEAQTAW
LQYMDRFGYDKNVYNTKSIVDLVKEHRAGKKVFPKNIDVVTGGFPCQDFSVSGKRLGFESNKDHTGKLREDDEASEETRG
KLYYWMKQVIDITRPKIFIAENVKGLVNLGDVKSIIQRDFAHAAGDGYIVLDPQVLHAGNYGVPESRERVIFIGIRKSAL
KVKALRALQEEKIPKAYDPYPSPTHACTVEDKKLLPPVVCRDVFVGLEEPDRTEDPSQRYYSQAKYMGAHCQGQKEIALD
NIGPTIRSEHHGNIEYRRLSREHGGVITEELDAGLQERRLTPRECALIQTFPPDYTFVSHKKNSTRFELSASGAYKVIGN
AVPPVLAYHIANRIQTLWPLYFKKR

Specific function: This methylase recognizes the double-stranded sequence CGCG, causes specific methylation on C-1 on both strands, and protects the DNA from cleavage by the BepI endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1788271, Length=436, Percent_Identity=25.9174311926606, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 48470; Mature: 48470

Theoretical pI: Translated: 8.60; Mature: 8.60

Prosite motif: PS00094 C5_MTASE_1 ; PS00095 C5_MTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMSEKEVDTSNELRVLSLFSGCGGMDLGFEGDFICHRKSISPQSTWVQEVIDEHWVRLQ
CCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHH
KNRFRTVFANDILKEAQTAWLQYMDRFGYDKNVYNTKSIVDLVKEHRAGKKVFPKNIDVV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEE
TGGFPCQDFSVSGKRLGFESNKDHTGKLREDDEASEETRGKLYYWMKQVIDITRPKIFIA
ECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHCCCEEEEE
ENVKGLVNLGDVKSIIQRDFAHAAGDGYIVLDPQVLHAGNYGVPESRERVIFIGIRKSAL
CCCCHHHCHHHHHHHHHHHHHHHCCCCEEEECCCEEECCCCCCCCCCCEEEEEEECCHHH
KVKALRALQEEKIPKAYDPYPSPTHACTVEDKKLLPPVVCRDVFVGLEEPDRTEDPSQRY
HHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHH
YSQAKYMGAHCQGQKEIALDNIGPTIRSEHHGNIEYRRLSREHGGVITEELDAGLQERRL
HHHHHHHCCCCCCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHCCEEHHHHHCCHHHHCC
TPRECALIQTFPPDYTFVSHKKNSTRFELSASGAYKVIGNAVPPVLAYHIANRIQTLWPL
CHHHEEEEEECCCCCEEEECCCCCCEEEEECCCCHHHHCCCCHHHHHHHHHHHHHHHHHH
YFKKR
HHCCC
>Mature Secondary Structure
MKMSEKEVDTSNELRVLSLFSGCGGMDLGFEGDFICHRKSISPQSTWVQEVIDEHWVRLQ
CCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHH
KNRFRTVFANDILKEAQTAWLQYMDRFGYDKNVYNTKSIVDLVKEHRAGKKVFPKNIDVV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEE
TGGFPCQDFSVSGKRLGFESNKDHTGKLREDDEASEETRGKLYYWMKQVIDITRPKIFIA
ECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHCCCEEEEE
ENVKGLVNLGDVKSIIQRDFAHAAGDGYIVLDPQVLHAGNYGVPESRERVIFIGIRKSAL
CCCCHHHCHHHHHHHHHHHHHHHCCCCEEEECCCEEECCCCCCCCCCCEEEEEEECCHHH
KVKALRALQEEKIPKAYDPYPSPTHACTVEDKKLLPPVVCRDVFVGLEEPDRTEDPSQRY
HHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHH
YSQAKYMGAHCQGQKEIALDNIGPTIRSEHHGNIEYRRLSREHGGVITEELDAGLQERRL
HHHHHHHCCCCCCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHCCEEHHHHHCCHHHHCC
TPRECALIQTFPPDYTFVSHKKNSTRFELSASGAYKVIGNAVPPVLAYHIANRIQTLWPL
CHHHEEEEEECCCCCEEEECCCCCCEEEEECCCCHHHHCCCCHHHHHHHHHHHHHHHHHH
YFKKR
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2784204 [H]