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The map label for this gene is ispB [H]

Identifier: 150009558

GI number: 150009558

Start: 3555112

End: 3556086

Strand: Reverse

Name: ispB [H]

Synonym: BDI_2971

Alternate gene names: 150009558

Gene position: 3556086-3555112 (Counterclockwise)

Preceding gene: 150009562

Following gene: 150009551

Centisome position: 73.91

GC content: 50.05

Gene sequence:

>975_bases
ATGGATGAAAGAAGGAAAATAGAAGAACCGGTTTCCGCTGAATTTGAACGTTTCAATAAGGATTTCGAGGCATCTCTACG
CAGCGAGACGACCCGGTTACAATCGGCTATCGACGTTATACTGAATTCTACCGGGAAGCATGTGCGTCCGTTATTGGTGC
TCCTTACCGCCAAGGTCTGTGGACAGGTTACGGATAACACGATCAATTCCGCTGTCTTGCTGGAGCTTTTGCATACCGCC
ACGTTGATTCACGATGACGTGATCGATGAGACGAAGCAACGTCGTGGCGTTCCCTCATTGAACGCTATCTTCGATAACCG
TATTTCCGTGTTGGTAGGCGATTACGTGCTTTCTACCGCTTTGATCCGTTCCATACAAACCGGCGATTTGCGGATCGTCG
GGATTATCTCCAATCTGGGCAGGGACTTGTCCGAAGGTGAGATCAAGCAATTGGAGACCGCCGAGGAGAGTATCCTAGAC
GAGAATTGCTATCTGCAAGTGATCAAGAAGAAGACGGCTACCTTGCTTTCCGCTTGCACGGAGATCGGAGCCATTTCATC
GGGCGCCTCCGCGGAGGTGATCGCTCTTTGCCGTGAGTTTGGCGAGTATCTGGGGTATTGCTTCCAGATCAAGGATGATA
TCTTTGATTATTTCAAGGAGGCGAATATCGGTAAGCCGACGGGAAATGACATTCGTGAGGGTAAGGTTACGCTTCCATTA
TTATATGCCTTGCGGCAGGGTAGGGAAGAGGAGGCCGCCCGTTATCTGGATATGATCTTGCGGAAGGATTTCGGGGCGGA
GAATGTCGATTCTTTGATCGAGTTCGCCAAGGCGAACGGTGGAATCGAATATGCCGAGGCCCGCATGAAGGAATATCATG
ATAAGGCGGTCGATGTTCTTTTGAGATTGCCAGAGTCTGAGGCACGGACATCCTTGATCCAGCTGGCCGATTATATCATG
ACTCGCTCCAAATAG

Upstream 100 bases:

>100_bases
ACTTTTAACACGTTTTAGTGGGACAAAATCTGGGTAAATGTACGAAAAAACTCGCTTACTCGATAGTTTTTGTTACTTTT
GCGAATCTTTGCATGGAATT

Downstream 100 bases:

>100_bases
AAATTCCTTTATCTATTAGTTGTAGAGAGATGGCTCTTGTTCGTAAAAAAGTAAGTATGCTTCAATAGCCAATGAAGTTA
ACTTACTTGACGAAGTCAGT

Product: polyprenyl synthetase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MDERRKIEEPVSAEFERFNKDFEASLRSETTRLQSAIDVILNSTGKHVRPLLVLLTAKVCGQVTDNTINSAVLLELLHTA
TLIHDDVIDETKQRRGVPSLNAIFDNRISVLVGDYVLSTALIRSIQTGDLRIVGIISNLGRDLSEGEIKQLETAEESILD
ENCYLQVIKKKTATLLSACTEIGAISSGASAEVIALCREFGEYLGYCFQIKDDIFDYFKEANIGKPTGNDIREGKVTLPL
LYALRQGREEEAARYLDMILRKDFGAENVDSLIEFAKANGGIEYAEARMKEYHDKAVDVLLRLPESEARTSLIQLADYIM
TRSK

Sequences:

>Translated_324_residues
MDERRKIEEPVSAEFERFNKDFEASLRSETTRLQSAIDVILNSTGKHVRPLLVLLTAKVCGQVTDNTINSAVLLELLHTA
TLIHDDVIDETKQRRGVPSLNAIFDNRISVLVGDYVLSTALIRSIQTGDLRIVGIISNLGRDLSEGEIKQLETAEESILD
ENCYLQVIKKKTATLLSACTEIGAISSGASAEVIALCREFGEYLGYCFQIKDDIFDYFKEANIGKPTGNDIREGKVTLPL
LYALRQGREEEAARYLDMILRKDFGAENVDSLIEFAKANGGIEYAEARMKEYHDKAVDVLLRLPESEARTSLIQLADYIM
TRSK
>Mature_324_residues
MDERRKIEEPVSAEFERFNKDFEASLRSETTRLQSAIDVILNSTGKHVRPLLVLLTAKVCGQVTDNTINSAVLLELLHTA
TLIHDDVIDETKQRRGVPSLNAIFDNRISVLVGDYVLSTALIRSIQTGDLRIVGIISNLGRDLSEGEIKQLETAEESILD
ENCYLQVIKKKTATLLSACTEIGAISSGASAEVIALCREFGEYLGYCFQIKDDIFDYFKEANIGKPTGNDIREGKVTLPL
LYALRQGREEEAARYLDMILRKDFGAENVDSLIEFAKANGGIEYAEARMKEYHDKAVDVLLRLPESEARTSLIQLADYIM
TRSK

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=313, Percent_Identity=29.7124600638978, Blast_Score=145, Evalue=4e-35,
Organism=Escherichia coli, GI1789578, Length=320, Percent_Identity=33.4375, Blast_Score=188, Evalue=3e-49,
Organism=Escherichia coli, GI1786623, Length=297, Percent_Identity=27.2727272727273, Blast_Score=78, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17505681, Length=304, Percent_Identity=29.9342105263158, Blast_Score=137, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6319475, Length=301, Percent_Identity=32.5581395348837, Blast_Score=142, Evalue=7e-35,
Organism=Drosophila melanogaster, GI24651612, Length=326, Percent_Identity=29.1411042944785, Blast_Score=137, Evalue=8e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 36199; Mature: 36199

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2 ; PS00924 ASP_GLU_RACEMASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDERRKIEEPVSAEFERFNKDFEASLRSETTRLQSAIDVILNSTGKHVRPLLVLLTAKVC
CCCHHHHCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
GQVTDNTINSAVLLELLHTATLIHDDVIDETKQRRGVPSLNAIFDNRISVLVGDYVLSTA
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCHHHHHHHHHHHHHH
LIRSIQTGDLRIVGIISNLGRDLSEGEIKQLETAEESILDENCYLQVIKKKTATLLSACT
HHHHHCCCCEEEEEEHHHHCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
EIGAISSGASAEVIALCREFGEYLGYCFQIKDDIFDYFKEANIGKPTGNDIREGKVTLPL
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHH
LYALRQGREEEAARYLDMILRKDFGAENVDSLIEFAKANGGIEYAEARMKEYHDKAVDVL
HHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
LRLPESEARTSLIQLADYIMTRSK
HHCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDERRKIEEPVSAEFERFNKDFEASLRSETTRLQSAIDVILNSTGKHVRPLLVLLTAKVC
CCCHHHHCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
GQVTDNTINSAVLLELLHTATLIHDDVIDETKQRRGVPSLNAIFDNRISVLVGDYVLSTA
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCHHHHHHHHHHHHHH
LIRSIQTGDLRIVGIISNLGRDLSEGEIKQLETAEESILDENCYLQVIKKKTATLLSACT
HHHHHCCCCEEEEEEHHHHCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
EIGAISSGASAEVIALCREFGEYLGYCFQIKDDIFDYFKEANIGKPTGNDIREGKVTLPL
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHH
LYALRQGREEEAARYLDMILRKDFGAENVDSLIEFAKANGGIEYAEARMKEYHDKAVDVL
HHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
LRLPESEARTSLIQLADYIMTRSK
HHCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]