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The map label for this gene is gyaR [H]

Identifier: 150009546

GI number: 150009546

Start: 3541913

End: 3542872

Strand: Reverse

Name: gyaR [H]

Synonym: BDI_2959

Alternate gene names: 150009546

Gene position: 3542872-3541913 (Counterclockwise)

Preceding gene: 150009550

Following gene: 150009545

Centisome position: 73.64

GC content: 51.77

Gene sequence:

>960_bases
ATGAAGATATTAGTGACATACGACATGTTCCGCGAGGGATTTACCGAGTTGGAAAGTAAGTACGAGGTAACCTTTCCCGA
AGAAAGAGATTTTACGTACGAGGAGGTTTTCGAGATGATACCGGAGTACGATGTGCTCTGCTCCATGTTCGATTTCCCGG
TAAACAAAGAGTTGATCGACCATGCGTCGAAATTGCGGCTGATCGCTAACTACGCCGTTGGTTATAATAATATCGATGTG
GCCTACGCCCTCGAGAAAGGCTTGACGGTAGCGAATACGCCTGATCCTGTCACGGCCCCTACCGCCAATATCGCTTTAGG
CTTGATGCTGGATACCGCCCGGCGTATCACGGAATGTGATCGTAAGCTGCGTACGCTTGGTAAGGACATGAAGGTCGGTG
TACTGGAGAACCTCGGTATGCCGGTTACCGGACAGACCTTGGGCATTATCGGTATGGGACGTATCGGCAAGGCCTTGGCG
AAACGGGCGAATGCTTTGGGCATGGATGTGATCTACCACAATCGCCGTCAGCTTTGCATCGAGGACGAGACCAAATTGAA
TGTTACCTACGTCTCCAAGGAAGAGCTTCTGGCGAAAGCCGATTTCGTATCCTTGAACGCCCCTTATACCCCCGATACCT
ACCATATCCTCGGCGAGGAAGAGTTCAAGCAGATGAAGCCTTCCGCCATCCTGATCAACACGGCCCGTGGCCCGTTGGTG
GACGAGCATGCCTTGGTAAAAGCCTTGCGAGAAGGTACGATTCATGGTGCCGGTTTGGATGTATTCGAGTTTGGAGACTA
TCCTCTCCCCGAGTTATTGGAAATGGACAACGTGGTATTAACCCCTCATATCGGCACCCAGACGATGGAAACCCGTATCA
TTATGGCACGCACGGTATGCAACAACGTGATCGGTTTCTTGGAGGGCGACCGTCCCGTATCACGGGTACTCCGGCCATGA

Upstream 100 bases:

>100_bases
TATAGTTTAGTACCATAATAAATATATATTGGTCACAAATTTAGGAATTAATTGCCAATTGATTTGTATTTTTGCTTTTT
GAATAACAAATAGATCCAAG

Downstream 100 bases:

>100_bases
CGGCGGCGTTTATCCTATCCGAGCTACAGTCGGTCGGTACACCCGAGAAGGCTGTTCATTTGAGCCGTTTTTTCAAGACT
GGTCCCGGTCAATATGGCGA

Product: D-isomer specific 2-hydroxyacid dehydrogenase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MKILVTYDMFREGFTELESKYEVTFPEERDFTYEEVFEMIPEYDVLCSMFDFPVNKELIDHASKLRLIANYAVGYNNIDV
AYALEKGLTVANTPDPVTAPTANIALGLMLDTARRITECDRKLRTLGKDMKVGVLENLGMPVTGQTLGIIGMGRIGKALA
KRANALGMDVIYHNRRQLCIEDETKLNVTYVSKEELLAKADFVSLNAPYTPDTYHILGEEEFKQMKPSAILINTARGPLV
DEHALVKALREGTIHGAGLDVFEFGDYPLPELLEMDNVVLTPHIGTQTMETRIIMARTVCNNVIGFLEGDRPVSRVLRP

Sequences:

>Translated_319_residues
MKILVTYDMFREGFTELESKYEVTFPEERDFTYEEVFEMIPEYDVLCSMFDFPVNKELIDHASKLRLIANYAVGYNNIDV
AYALEKGLTVANTPDPVTAPTANIALGLMLDTARRITECDRKLRTLGKDMKVGVLENLGMPVTGQTLGIIGMGRIGKALA
KRANALGMDVIYHNRRQLCIEDETKLNVTYVSKEELLAKADFVSLNAPYTPDTYHILGEEEFKQMKPSAILINTARGPLV
DEHALVKALREGTIHGAGLDVFEFGDYPLPELLEMDNVVLTPHIGTQTMETRIIMARTVCNNVIGFLEGDRPVSRVLRP
>Mature_319_residues
MKILVTYDMFREGFTELESKYEVTFPEERDFTYEEVFEMIPEYDVLCSMFDFPVNKELIDHASKLRLIANYAVGYNNIDV
AYALEKGLTVANTPDPVTAPTANIALGLMLDTARRITECDRKLRTLGKDMKVGVLENLGMPVTGQTLGIIGMGRIGKALA
KRANALGMDVIYHNRRQLCIEDETKLNVTYVSKEELLAKADFVSLNAPYTPDTYHILGEEEFKQMKPSAILINTARGPLV
DEHALVKALREGTIHGAGLDVFEFGDYPLPELLEMDNVVLTPHIGTQTMETRIIMARTVCNNVIGFLEGDRPVSRVLRP

Specific function: Unknown

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GyaR subfamily [H]

Homologues:

Organism=Homo sapiens, GI6912396, Length=272, Percent_Identity=34.5588235294118, Blast_Score=151, Evalue=7e-37,
Organism=Homo sapiens, GI23308577, Length=266, Percent_Identity=30.8270676691729, Blast_Score=139, Evalue=4e-33,
Organism=Homo sapiens, GI61743967, Length=290, Percent_Identity=31.7241379310345, Blast_Score=132, Evalue=5e-31,
Organism=Homo sapiens, GI4557497, Length=293, Percent_Identity=32.0819112627986, Blast_Score=131, Evalue=7e-31,
Organism=Homo sapiens, GI145580578, Length=293, Percent_Identity=31.3993174061433, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI4557499, Length=293, Percent_Identity=31.3993174061433, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI145580575, Length=291, Percent_Identity=31.6151202749141, Blast_Score=129, Evalue=5e-30,
Organism=Escherichia coli, GI87082289, Length=260, Percent_Identity=42.6923076923077, Blast_Score=190, Evalue=1e-49,
Organism=Escherichia coli, GI1789279, Length=274, Percent_Identity=33.9416058394161, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1787645, Length=272, Percent_Identity=30.5147058823529, Blast_Score=120, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17532191, Length=310, Percent_Identity=29.0322580645161, Blast_Score=134, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI25147481, Length=267, Percent_Identity=28.0898876404494, Blast_Score=98, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6324055, Length=243, Percent_Identity=37.8600823045267, Blast_Score=156, Evalue=5e-39,
Organism=Saccharomyces cerevisiae, GI6320925, Length=306, Percent_Identity=31.3725490196078, Blast_Score=135, Evalue=6e-33,
Organism=Saccharomyces cerevisiae, GI6322116, Length=312, Percent_Identity=30.4487179487179, Blast_Score=135, Evalue=9e-33,
Organism=Saccharomyces cerevisiae, GI6321253, Length=325, Percent_Identity=25.5384615384615, Blast_Score=71, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324964, Length=272, Percent_Identity=24.6323529411765, Blast_Score=71, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6325144, Length=196, Percent_Identity=28.0612244897959, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI28571528, Length=320, Percent_Identity=34.6875, Blast_Score=155, Evalue=3e-38,
Organism=Drosophila melanogaster, GI45552429, Length=316, Percent_Identity=32.5949367088608, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI45551003, Length=316, Percent_Identity=32.5949367088608, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24585514, Length=316, Percent_Identity=32.5949367088608, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI28574282, Length=316, Percent_Identity=32.5949367088608, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI28574284, Length=316, Percent_Identity=32.5949367088608, Blast_Score=149, Evalue=2e-36,
Organism=Drosophila melanogaster, GI28574286, Length=264, Percent_Identity=34.8484848484849, Blast_Score=148, Evalue=6e-36,
Organism=Drosophila melanogaster, GI24585516, Length=298, Percent_Identity=32.2147651006711, Blast_Score=142, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24646446, Length=237, Percent_Identity=35.4430379746835, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24646448, Length=237, Percent_Identity=35.4430379746835, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24646452, Length=237, Percent_Identity=35.4430379746835, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24646450, Length=237, Percent_Identity=35.4430379746835, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI62472511, Length=236, Percent_Identity=34.7457627118644, Blast_Score=118, Evalue=6e-27,
Organism=Drosophila melanogaster, GI19921140, Length=323, Percent_Identity=26.0061919504644, Blast_Score=113, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.26 [H]

Molecular weight: Translated: 35754; Mature: 35754

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1 ; PS00670 D_2_HYDROXYACID_DH_2 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVTYDMFREGFTELESKYEVTFPEERDFTYEEVFEMIPEYDVLCSMFDFPVNKELID
CEEEEEHHHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHCCCHHHHHHHHCCCCCHHHHH
HASKLRLIANYAVGYNNIDVAYALEKGLTVANTPDPVTAPTANIALGLMLDTARRITECD
HHHHHHHHHHHHCCCCCCEEEEEHHCCCEECCCCCCCCCCCHHHEEHHHHHHHHHHHHHH
RKLRTLGKDMKVGVLENLGMPVTGQTLGIIGMGRIGKALAKRANALGMDVIYHNRRQLCI
HHHHHHCCCCEEHHHHHCCCCCCCCEEEEEECHHHHHHHHHHHHHCCHHEEEECCCCEEE
EDETKLNVTYVSKEELLAKADFVSLNAPYTPDTYHILGEEEFKQMKPSAILINTARGPLV
CCCCEEEEEEECHHHHHHHHCEEECCCCCCCCCEEECCHHHHHHCCCCEEEEECCCCCCC
DEHALVKALREGTIHGAGLDVFEFGDYPLPELLEMDNVVLTPHIGTQTMETRIIMARTVC
HHHHHHHHHHHCCCCCCCCCCEECCCCCCHHHHCCCCEEECCCCCCHHHHHHHHHHHHHH
NNVIGFLEGDRPVSRVLRP
HHHHHHCCCCCHHHHHCCC
>Mature Secondary Structure
MKILVTYDMFREGFTELESKYEVTFPEERDFTYEEVFEMIPEYDVLCSMFDFPVNKELID
CEEEEEHHHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHCCCHHHHHHHHCCCCCHHHHH
HASKLRLIANYAVGYNNIDVAYALEKGLTVANTPDPVTAPTANIALGLMLDTARRITECD
HHHHHHHHHHHHCCCCCCEEEEEHHCCCEECCCCCCCCCCCHHHEEHHHHHHHHHHHHHH
RKLRTLGKDMKVGVLENLGMPVTGQTLGIIGMGRIGKALAKRANALGMDVIYHNRRQLCI
HHHHHHCCCCEEHHHHHCCCCCCCCEEEEEECHHHHHHHHHHHHHCCHHEEEECCCCEEE
EDETKLNVTYVSKEELLAKADFVSLNAPYTPDTYHILGEEEFKQMKPSAILINTARGPLV
CCCCEEEEEEECHHHHHHHHCEEECCCCCCCCCEEECCHHHHHHCCCCEEEEECCCCCCC
DEHALVKALREGTIHGAGLDVFEFGDYPLPELLEMDNVVLTPHIGTQTMETRIIMARTVC
HHHHHHHHHHHCCCCCCCCCCEECCCCCCHHHHCCCCEEECCCCCCHHHHHHHHHHHHHH
NNVIGFLEGDRPVSRVLRP
HHHHHHCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA