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The map label for this gene is ply [H]

Identifier: 150009541

GI number: 150009541

Start: 3537307

End: 3538875

Strand: Direct

Name: ply [H]

Synonym: BDI_2954

Alternate gene names: 150009541

Gene position: 3537307-3538875 (Clockwise)

Preceding gene: 150009540

Following gene: 150009547

Centisome position: 73.52

GC content: 31.93

Gene sequence:

>1569_bases
ATGAGAATTACATCAATATTTTTATTTACAATCATCATATTTGGAGGATTATCATGTTCAAATGATGATAGCCTTATTCA
TAATAAGACTAAAGGAATCTTAGAGAAATATCCTGAACCCACTGTCACAATAACAGCTCATAATCATATACCTTTTCTTG
CTAAAAATATTTTAGAAGAAAAAGCGATTACAAAAAGTGTAAATATTATCTCCGATATTGCATGGGCAAATGGATTTGGA
AGAACAATTACAGCCCGATCGGATGAAATGGTAATACCAGACAATATGAATAGATACATATACCCGGGATCAATATTGGA
AGCATCTAGCATTGCAGAGACACGATTTACTCCAATACCTGTAAAAAACAATCCGGTCCATGTCTCTGTCTCTTTTCCTG
CTAAAAAAGTAGGAGGAACTATAAAAGAGCCTTCTTTATTGAATATCAGACAATTTGTCATGGATCTAATGCAACAAAAT
AATATAGGAAAACAAAGTGCAACATTAAGTTTTGATGTTCAAAAATTTGTATCATATGATGAATTAAAAATGACTTTTGG
CTCTAATGAAAATACAGGATTATTGTTTTGGGGAACTTCTTCTGCGCAGTATCAAAATAAATATAGAATCATACGCAGCT
CTGGACTTTGCATAAAATTTATTCAGAAATATTTCACATTAGACATGGATATTCCTAGTAATGGTCTAATTTCAGGAACT
ATACCAGGAGGATACTCACCTGTATACGTTAGCTCTATTGCCTATGGAAGAATAGGTATTCTCACACTAGAAACAAATTA
CGATTATGAAAAAGCTAACAAATTAGTGAAAGAAACTTTTAATTCGCTATTTATTAACAAGAATAATACACTCACAAAGG
AGCAAGAAGCCTTTTTCAATAGCGCAGAAATGAAAGTTTTCATAGCAGGAGGATCAGGGGTCACAGGAGTTAAAACTATA
GGAGGTATTAAAGAATTCACAAATTATATTACAGAGGGAGGAGAGTTTTCAGCATCCTCACCAGGTAAACCAATTTTTTG
TTCTTTTGCAAACTATAGTGACGATTCTCCATATCGCATTAATTTCAAAATAGATATAGACAGTGATCCTGTTTATGCAA
GACTTGAATATGATATAACCAAAGATGTATCAGGTGGAAATGTTAAATCATGGTATACTGATGCTAATGTTTATTTAAGG
TTTTATGCAGATAAAGCCTGTTCAATCAAAACGATAGCCCAAAACTATATTGGATTTAATATAAATCGCCTTATATTACA
ACAATATGTTGGACCTAATTATAGTGTTAGTAAAGCAAGAGAGGTAAAACTAGATCAAAACCTCACTTTTCATAATCAAA
CAAAAGGCATAGAGTATCCAATTGCATATGGTCATCGCATGACAGAAACAATAGGCACTACATATTTAGATCGAGGAAAT
TGGACTAATGACCATTTTTACCTAAGAGCAGGTAAATTTTATCAAGTACGACCTCCTATAAATATGAAATATACATGTTT
CATAGATTATCATAATCAAAATGAATATGACTGTTCGGATTTTGAATAA

Upstream 100 bases:

>100_bases
GTTCCTGGAGATCCTATATTTTATTCAGCAAGCTACTTAAGTGATGATTCTCCATTCTACGCAAAATTCAGAGTAAACAT
ACCTTATAAATAATTATACT

Downstream 100 bases:

>100_bases
TTAATATTCCAAAATAAAATACCCCTAGAGTTAAATAGACAACTTTGGGGGTATTTCTATCATAGGAGCTTGAACCTCTG
TATATATTAAGATTAATATA

Product: putative hemolysin

Products: NA

Alternate protein names: Thiol-activated cytolysin [H]

Number of amino acids: Translated: 522; Mature: 522

Protein sequence:

>522_residues
MRITSIFLFTIIIFGGLSCSNDDSLIHNKTKGILEKYPEPTVTITAHNHIPFLAKNILEEKAITKSVNIISDIAWANGFG
RTITARSDEMVIPDNMNRYIYPGSILEASSIAETRFTPIPVKNNPVHVSVSFPAKKVGGTIKEPSLLNIRQFVMDLMQQN
NIGKQSATLSFDVQKFVSYDELKMTFGSNENTGLLFWGTSSAQYQNKYRIIRSSGLCIKFIQKYFTLDMDIPSNGLISGT
IPGGYSPVYVSSIAYGRIGILTLETNYDYEKANKLVKETFNSLFINKNNTLTKEQEAFFNSAEMKVFIAGGSGVTGVKTI
GGIKEFTNYITEGGEFSASSPGKPIFCSFANYSDDSPYRINFKIDIDSDPVYARLEYDITKDVSGGNVKSWYTDANVYLR
FYADKACSIKTIAQNYIGFNINRLILQQYVGPNYSVSKAREVKLDQNLTFHNQTKGIEYPIAYGHRMTETIGTTYLDRGN
WTNDHFYLRAGKFYQVRPPINMKYTCFIDYHNQNEYDCSDFE

Sequences:

>Translated_522_residues
MRITSIFLFTIIIFGGLSCSNDDSLIHNKTKGILEKYPEPTVTITAHNHIPFLAKNILEEKAITKSVNIISDIAWANGFG
RTITARSDEMVIPDNMNRYIYPGSILEASSIAETRFTPIPVKNNPVHVSVSFPAKKVGGTIKEPSLLNIRQFVMDLMQQN
NIGKQSATLSFDVQKFVSYDELKMTFGSNENTGLLFWGTSSAQYQNKYRIIRSSGLCIKFIQKYFTLDMDIPSNGLISGT
IPGGYSPVYVSSIAYGRIGILTLETNYDYEKANKLVKETFNSLFINKNNTLTKEQEAFFNSAEMKVFIAGGSGVTGVKTI
GGIKEFTNYITEGGEFSASSPGKPIFCSFANYSDDSPYRINFKIDIDSDPVYARLEYDITKDVSGGNVKSWYTDANVYLR
FYADKACSIKTIAQNYIGFNINRLILQQYVGPNYSVSKAREVKLDQNLTFHNQTKGIEYPIAYGHRMTETIGTTYLDRGN
WTNDHFYLRAGKFYQVRPPINMKYTCFIDYHNQNEYDCSDFE
>Mature_522_residues
MRITSIFLFTIIIFGGLSCSNDDSLIHNKTKGILEKYPEPTVTITAHNHIPFLAKNILEEKAITKSVNIISDIAWANGFG
RTITARSDEMVIPDNMNRYIYPGSILEASSIAETRFTPIPVKNNPVHVSVSFPAKKVGGTIKEPSLLNIRQFVMDLMQQN
NIGKQSATLSFDVQKFVSYDELKMTFGSNENTGLLFWGTSSAQYQNKYRIIRSSGLCIKFIQKYFTLDMDIPSNGLISGT
IPGGYSPVYVSSIAYGRIGILTLETNYDYEKANKLVKETFNSLFINKNNTLTKEQEAFFNSAEMKVFIAGGSGVTGVKTI
GGIKEFTNYITEGGEFSASSPGKPIFCSFANYSDDSPYRINFKIDIDSDPVYARLEYDITKDVSGGNVKSWYTDANVYLR
FYADKACSIKTIAQNYIGFNINRLILQQYVGPNYSVSKAREVKLDQNLTFHNQTKGIEYPIAYGHRMTETIGTTYLDRGN
WTNDHFYLRAGKFYQVRPPINMKYTCFIDYHNQNEYDCSDFE

Specific function: Sulfhydryl-activated toxin that causes cytolysis by forming pores in cholesterol containing host membranes. After binding to target membranes, the protein undergoes a major conformation change, leading to its insertion in the host membrane and formation o

COG id: NA

COG function: NA

Gene ontology:

Cell location: Secreted. Host cell membrane; Multi-pass membrane protein (By similarity). Note=Secreted as soluble protein that then inserts into the host cell membrane and forms pores formed by transmembrane beta-strands (By similarity) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiol-activated cytolysin family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001869 [H]

Pfam domain/function: PF01289 Thiol_cytolysin [H]

EC number: NA

Molecular weight: Translated: 58912; Mature: 58912

Theoretical pI: Translated: 8.07; Mature: 8.07

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRITSIFLFTIIIFGGLSCSNDDSLIHNKTKGILEKYPEPTVTITAHNHIPFLAKNILEE
CEEHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHH
KAITKSVNIISDIAWANGFGRTITARSDEMVIPDNMNRYIYPGSILEASSIAETRFTPIP
HHHHHHHHHHHHHHHCCCCCCEEEECCCCEECCCCCCCEECCCCCCCCCHHHHCCCCCCC
VKNNPVHVSVSFPAKKVGGTIKEPSLLNIRQFVMDLMQQNNIGKQSATLSFDVQKFVSYD
CCCCCEEEEEECCHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHCCCC
ELKMTFGSNENTGLLFWGTSSAQYQNKYRIIRSSGLCIKFIQKYFTLDMDIPSNGLISGT
CEEEEECCCCCCEEEEECCCCCCCCCEEEEEECCCHHHHHHHHHHEEEECCCCCCEEEEC
IPGGYSPVYVSSIAYGRIGILTLETNYDYEKANKLVKETFNSLFINKNNTLTKEQEAFFN
CCCCCCCEEEEEECCCEEEEEEEECCCCHHHHHHHHHHHHHHEEEECCCCCCCHHHHHCC
SAEMKVFIAGGSGVTGVKTIGGIKEFTNYITEGGEFSASSPGKPIFCSFANYSDDSPYRI
CCCEEEEEECCCCCCCHHHHCCHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCEEE
NFKIDIDSDPVYARLEYDITKDVSGGNVKSWYTDANVYLRFYADKACSIKTIAQNYIGFN
EEEEEECCCCEEEEEEEEEEECCCCCCCEEEEECCEEEEEEEECCCCCHHHHHHHHCCCC
INRLILQQYVGPNYSVSKAREVKLDQNLTFHNQTKGIEYPIAYGHRMTETIGTTYLDRGN
HHHHHHHHHCCCCCCCCCCEEEEECCCCEECCCCCCEECCHHCCCHHHHHHCCEEECCCC
WTNDHFYLRAGKFYQVRPPINMKYTCFIDYHNQNEYDCSDFE
CCCCEEEEEECCEEEECCCCCEEEEEEEEECCCCCCCCCCCC
>Mature Secondary Structure
MRITSIFLFTIIIFGGLSCSNDDSLIHNKTKGILEKYPEPTVTITAHNHIPFLAKNILEE
CEEHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHH
KAITKSVNIISDIAWANGFGRTITARSDEMVIPDNMNRYIYPGSILEASSIAETRFTPIP
HHHHHHHHHHHHHHHCCCCCCEEEECCCCEECCCCCCCEECCCCCCCCCHHHHCCCCCCC
VKNNPVHVSVSFPAKKVGGTIKEPSLLNIRQFVMDLMQQNNIGKQSATLSFDVQKFVSYD
CCCCCEEEEEECCHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHCCCC
ELKMTFGSNENTGLLFWGTSSAQYQNKYRIIRSSGLCIKFIQKYFTLDMDIPSNGLISGT
CEEEEECCCCCCEEEEECCCCCCCCCEEEEEECCCHHHHHHHHHHEEEECCCCCCEEEEC
IPGGYSPVYVSSIAYGRIGILTLETNYDYEKANKLVKETFNSLFINKNNTLTKEQEAFFN
CCCCCCCEEEEEECCCEEEEEEEECCCCHHHHHHHHHHHHHHEEEECCCCCCCHHHHHCC
SAEMKVFIAGGSGVTGVKTIGGIKEFTNYITEGGEFSASSPGKPIFCSFANYSDDSPYRI
CCCEEEEEECCCCCCCHHHHCCHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCEEE
NFKIDIDSDPVYARLEYDITKDVSGGNVKSWYTDANVYLRFYADKACSIKTIAQNYIGFN
EEEEEECCCCEEEEEEEEEEECCCCCCCEEEEECCEEEEEEEECCCCCHHHHHHHHCCCC
INRLILQQYVGPNYSVSKAREVKLDQNLTFHNQTKGIEYPIAYGHRMTETIGTTYLDRGN
HHHHHHHHHCCCCCCCCCCEEEEECCCCEECCCCCCEECCHHCCCHHHHHHCCEEECCCC
WTNDHFYLRAGKFYQVRPPINMKYTCFIDYHNQNEYDCSDFE
CCCCEEEEEECCEEEECCCCCEEEEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3552992 [H]