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The map label for this gene is cysS

Identifier: 150009429

GI number: 150009429

Start: 3386343

End: 3387818

Strand: Direct

Name: cysS

Synonym: BDI_2837

Alternate gene names: 150009429

Gene position: 3386343-3387818 (Clockwise)

Preceding gene: 150009428

Following gene: 150009430

Centisome position: 70.38

GC content: 49.46

Gene sequence:

>1476_bases
ATGGAACATCCATTGAATATTTACAACACGCTTACGAGAAAGAAGGAGCAATTCATACCCCTGCACGAGCCGCATGTAGG
TATGTACGTATGTGGCCCCACGGTTTATGGTGACGCCCACTTGGGACACGCACGTCCGGCGATCACGTTCGACTTGCTAT
TCCGTTACCTTACCCACCTAGGATATAAAGTGCGTTATGTACGTAATATAACGGATGTAGGGCATCTGGAGCACGACGCC
GATGATGGGGAGGATAAGATCGCCAAGAAGGCACGGCTAGAACAGTTGGAGCCGATGGAAGTGGTTCAATATTACCTCAA
TCGTTATCATCAAGCGATGGATGCGCTGAACGTACTTCCTCCCAGCATCGAGCCGCACGCTTCCGGACATATTATCGAAC
AAATCGAGTTAGTCAAGAAGATTCTGGATAACGGCTACGCTTACGAAAGTCAAGGCTCCGTCTATTTCGACGTAGAGAAA
TATAATAAAGATCATAAATACGGCATCCTCTCGGGTCGCAATATCGAGGATATGTTGAATACGACCCGCGCGTTGGACGG
GCAGGACGAGAAGCATAACGCCATCGACTTCGCCCTTTGGAAATGCGCCCAGCCGGAGCATATCATGCGTTGGCCGTCTC
CGTGGAGCGACGGCTTCCCCGGTTGGCACTGCGAATGTACCGCTATGGGCAAGAAATACCTCGGGGAGCATTTCGACATC
CACGGAGGTGGCATGGACTTGATCTTCCCGCACCATGAATGCGAGATCGCACAAGCCGTGGCTTCCCAAGGAGATGATAT
GGTTCATTATTGGATGCACAACAATATGATCACCATCAACGGGCAGAAGATGGGTAAATCATTAGGTAATTTCATCACGC
TGGAGCAATTCTTCACGGGTGATCATCCCTCCTTGCAACAAGCGTATTCCGCCATGACCATCCGTTTCTTCATCCTGCAA
GCGCATTACCGTAGCACGGTCGATTTCAGCAACGAGGCTTTACAGGCTGCCGAGAAGGGCTTGTCACGCTTGATGGAGGC
ATACGGACATTTGATGAAGTTGCAGCCGTCCGCTACCTCTACCGTAGACACGAAAGGTCTTCGTGAGAAATGTTTCGAGG
CCATGAATGACGATTTGAACTCACCAATCGTGATTTCTCATCTATTCGACGCTACCCGTGCGATCAATTCCGTAAAAGAC
GGTAAGGCTACTCTGTCCGAGGAAGACTTGAAAGAGTTACAAGAGGTATTCCATTTATTCTTGTTCGATATCCTTGGCAT
GAAAGACGAGGCTTCCGCCAGCGGCAACCATTACGAGGCGTTTGGCAAAGCCGTGGATTTATTGCTGTCCATCCGCCAGC
AAGCCAAGGCAAACAAAGACTGGGCAACTTCCGATAAAATCCGTAACGAATTAACCGCTATGGGATTCGAGATCAAGGAT
ACCAAGGATGGAGCGGAATGGAAACTAAGCAAATAA

Upstream 100 bases:

>100_bases
GAGTCAATGAATGCCTATGAAAACTACAGACTATTCACGATCAGCTTGATAATACCAAGGATTTTATGTAAGTTCGCGAT
TCCTAAATATATAATAGAAT

Downstream 100 bases:

>100_bases
TCCACCAAGCGAATAGTAAGAGGTTATGACAATCCATGAATTTCTTCAAGGCGATAAATTCGCCTTGCTTGCGGGCGTTG
AATTATTAGAAACGGGCAAC

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MEHPLNIYNTLTRKKEQFIPLHEPHVGMYVCGPTVYGDAHLGHARPAITFDLLFRYLTHLGYKVRYVRNITDVGHLEHDA
DDGEDKIAKKARLEQLEPMEVVQYYLNRYHQAMDALNVLPPSIEPHASGHIIEQIELVKKILDNGYAYESQGSVYFDVEK
YNKDHKYGILSGRNIEDMLNTTRALDGQDEKHNAIDFALWKCAQPEHIMRWPSPWSDGFPGWHCECTAMGKKYLGEHFDI
HGGGMDLIFPHHECEIAQAVASQGDDMVHYWMHNNMITINGQKMGKSLGNFITLEQFFTGDHPSLQQAYSAMTIRFFILQ
AHYRSTVDFSNEALQAAEKGLSRLMEAYGHLMKLQPSATSTVDTKGLREKCFEAMNDDLNSPIVISHLFDATRAINSVKD
GKATLSEEDLKELQEVFHLFLFDILGMKDEASASGNHYEAFGKAVDLLLSIRQQAKANKDWATSDKIRNELTAMGFEIKD
TKDGAEWKLSK

Sequences:

>Translated_491_residues
MEHPLNIYNTLTRKKEQFIPLHEPHVGMYVCGPTVYGDAHLGHARPAITFDLLFRYLTHLGYKVRYVRNITDVGHLEHDA
DDGEDKIAKKARLEQLEPMEVVQYYLNRYHQAMDALNVLPPSIEPHASGHIIEQIELVKKILDNGYAYESQGSVYFDVEK
YNKDHKYGILSGRNIEDMLNTTRALDGQDEKHNAIDFALWKCAQPEHIMRWPSPWSDGFPGWHCECTAMGKKYLGEHFDI
HGGGMDLIFPHHECEIAQAVASQGDDMVHYWMHNNMITINGQKMGKSLGNFITLEQFFTGDHPSLQQAYSAMTIRFFILQ
AHYRSTVDFSNEALQAAEKGLSRLMEAYGHLMKLQPSATSTVDTKGLREKCFEAMNDDLNSPIVISHLFDATRAINSVKD
GKATLSEEDLKELQEVFHLFLFDILGMKDEASASGNHYEAFGKAVDLLLSIRQQAKANKDWATSDKIRNELTAMGFEIKD
TKDGAEWKLSK
>Mature_491_residues
MEHPLNIYNTLTRKKEQFIPLHEPHVGMYVCGPTVYGDAHLGHARPAITFDLLFRYLTHLGYKVRYVRNITDVGHLEHDA
DDGEDKIAKKARLEQLEPMEVVQYYLNRYHQAMDALNVLPPSIEPHASGHIIEQIELVKKILDNGYAYESQGSVYFDVEK
YNKDHKYGILSGRNIEDMLNTTRALDGQDEKHNAIDFALWKCAQPEHIMRWPSPWSDGFPGWHCECTAMGKKYLGEHFDI
HGGGMDLIFPHHECEIAQAVASQGDDMVHYWMHNNMITINGQKMGKSLGNFITLEQFFTGDHPSLQQAYSAMTIRFFILQ
AHYRSTVDFSNEALQAAEKGLSRLMEAYGHLMKLQPSATSTVDTKGLREKCFEAMNDDLNSPIVISHLFDATRAINSVKD
GKATLSEEDLKELQEVFHLFLFDILGMKDEASASGNHYEAFGKAVDLLLSIRQQAKANKDWATSDKIRNELTAMGFEIKD
TKDGAEWKLSK

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=534, Percent_Identity=32.3970037453184, Blast_Score=231, Evalue=1e-60,
Organism=Homo sapiens, GI303304987, Length=377, Percent_Identity=31.8302387267904, Blast_Score=183, Evalue=2e-46,
Organism=Homo sapiens, GI21269877, Length=377, Percent_Identity=31.8302387267904, Blast_Score=183, Evalue=3e-46,
Organism=Homo sapiens, GI10835051, Length=377, Percent_Identity=31.8302387267904, Blast_Score=183, Evalue=3e-46,
Organism=Homo sapiens, GI62240992, Length=377, Percent_Identity=31.8302387267904, Blast_Score=183, Evalue=3e-46,
Organism=Escherichia coli, GI1786737, Length=488, Percent_Identity=39.344262295082, Blast_Score=329, Evalue=2e-91,
Organism=Caenorhabditis elegans, GI17509797, Length=254, Percent_Identity=35.4330708661417, Blast_Score=152, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI17509795, Length=254, Percent_Identity=35.4330708661417, Blast_Score=152, Evalue=4e-37,
Organism=Saccharomyces cerevisiae, GI6324082, Length=336, Percent_Identity=31.547619047619, Blast_Score=162, Evalue=1e-40,
Organism=Drosophila melanogaster, GI19922218, Length=525, Percent_Identity=29.7142857142857, Blast_Score=201, Evalue=9e-52,
Organism=Drosophila melanogaster, GI19922364, Length=247, Percent_Identity=35.6275303643725, Blast_Score=167, Evalue=1e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_PARD8 (A6LFT6)

Other databases:

- EMBL:   CP000140
- RefSeq:   YP_001304172.1
- ProteinModelPortal:   A6LFT6
- SMR:   A6LFT6
- STRING:   A6LFT6
- GeneID:   5307986
- GenomeReviews:   CP000140_GR
- KEGG:   pdi:BDI_2837
- eggNOG:   COG0215
- HOGENOM:   HBG327651
- OMA:   DFDALNM
- ProtClustDB:   PRK00260
- BioCyc:   PDIS435591:BDI_2837-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 55871; Mature: 55871

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 286-286

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEHPLNIYNTLTRKKEQFIPLHEPHVGMYVCGPTVYGDAHLGHARPAITFDLLFRYLTHL
CCCCHHHHHHHHHHHHHCCCCCCCCCCEEEECCEEECCCCCCCCCCHHHHHHHHHHHHHC
GYKVRYVRNITDVGHLEHDADDGEDKIAKKARLEQLEPMEVVQYYLNRYHQAMDALNVLP
CCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCC
PSIEPHASGHIIEQIELVKKILDNGYAYESQGSVYFDVEKYNKDHKYGILSGRNIEDMLN
CCCCCCCCCHHHHHHHHHHHHHHCCCEEECCCCEEEEHHHCCCCCCEEECCCCCHHHHHH
TTRALDGQDEKHNAIDFALWKCAQPEHIMRWPSPWSDGFPGWHCECTAMGKKYLGEHFDI
HHHHCCCCCCCCCHHHHHHHHCCCCHHHCCCCCCCCCCCCCCEEEHHHHHHHHCCCCCEE
HGGGMDLIFPHHECEIAQAVASQGDDMVHYWMHNNMITINGQKMGKSLGNFITLEQFFTG
CCCCEEEECCCHHHHHHHHHHHCCCCEEEEEEECCEEEECHHHHHHHHHCEEEHHHHHCC
DHPSLQQAYSAMTIRFFILQAHYRSTVDFSNEALQAAEKGLSRLMEAYGHLMKLQPSATS
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCC
TVDTKGLREKCFEAMNDDLNSPIVISHLFDATRAINSVKDGKATLSEEDLKELQEVFHLF
CCCHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
LFDILGMKDEASASGNHYEAFGKAVDLLLSIRQQAKANKDWATSDKIRNELTAMGFEIKD
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCEEEC
TKDGAEWKLSK
CCCCCCEECCC
>Mature Secondary Structure
MEHPLNIYNTLTRKKEQFIPLHEPHVGMYVCGPTVYGDAHLGHARPAITFDLLFRYLTHL
CCCCHHHHHHHHHHHHHCCCCCCCCCCEEEECCEEECCCCCCCCCCHHHHHHHHHHHHHC
GYKVRYVRNITDVGHLEHDADDGEDKIAKKARLEQLEPMEVVQYYLNRYHQAMDALNVLP
CCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCC
PSIEPHASGHIIEQIELVKKILDNGYAYESQGSVYFDVEKYNKDHKYGILSGRNIEDMLN
CCCCCCCCCHHHHHHHHHHHHHHCCCEEECCCCEEEEHHHCCCCCCEEECCCCCHHHHHH
TTRALDGQDEKHNAIDFALWKCAQPEHIMRWPSPWSDGFPGWHCECTAMGKKYLGEHFDI
HHHHCCCCCCCCCHHHHHHHHCCCCHHHCCCCCCCCCCCCCCEEEHHHHHHHHCCCCCEE
HGGGMDLIFPHHECEIAQAVASQGDDMVHYWMHNNMITINGQKMGKSLGNFITLEQFFTG
CCCCEEEECCCHHHHHHHHHHHCCCCEEEEEEECCEEEECHHHHHHHHHCEEEHHHHHCC
DHPSLQQAYSAMTIRFFILQAHYRSTVDFSNEALQAAEKGLSRLMEAYGHLMKLQPSATS
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCC
TVDTKGLREKCFEAMNDDLNSPIVISHLFDATRAINSVKDGKATLSEEDLKELQEVFHLF
CCCHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
LFDILGMKDEASASGNHYEAFGKAVDLLLSIRQQAKANKDWATSDKIRNELTAMGFEIKD
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCEEEC
TKDGAEWKLSK
CCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA