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The map label for this gene is pckA [H]

Identifier: 150009397

GI number: 150009397

Start: 3345655

End: 3347262

Strand: Reverse

Name: pckA [H]

Synonym: BDI_2805

Alternate gene names: 150009397

Gene position: 3347262-3345655 (Counterclockwise)

Preceding gene: 150009398

Following gene: 150009396

Centisome position: 69.57

GC content: 44.9

Gene sequence:

>1608_bases
ATGGCTAATTTAGATTTAAGCAAGTACGGAATTGTTGATGTGAAAGAAATCGTTCACAATCCGTCTTATGATGTTCTGTT
CGCAGAAGAAACTAAACCGAGTTTGGAAGGCTTCGAGAAAGGCCAAGTAACTGAGCTAGGTGCTGTAAACGTGATGACTG
GTATTTATACAGGTCGTTCTCCTAAAGATAAATTTTTCGTAAAGAATGAAGCCAGCGAAGATTCTGTATGGTGGACTTCT
GATGAATATAAGAATGATAACAAGCCTTGTTCTGAGGCCGCTTGGGCAGACTTGAAGGCTAAAGCCGTAAAAGAATTGTC
TGGCAAACGTTTGTTCGTTGTCGATACATTCTGTGGCGCTAACGAGGCTACTCGTTTGAAAGTACGTTTCATCATGGAGG
TTGCTTGGCAAGCTCACTTCGTAACTAATATGTTCATCCGTCCGACAGCTGAGGAATTGGCTAACTACGGCGAACCTGAT
TTCGTTTCTTTCAACGCTGCTAAGGCTAAAGTTGACAACTACAAAGAATTAGGCTTGAACTCTGAGACTGCTACTGTTTT
CAACTTGAAGACTAAAGAGCAAGTTATCTTGAATACTTGGTACGGTGGTGAGATGAAGAAGGGTATCTTCTCTATCATGA
ACTACTTGAACCCGTTGCGTGGTATCGCTTCTATGCACTGCTCCGCTAACACGGATAAAGAGGGCAAGAGCTCAGCTATC
TTCTTCGGTTTGTCTGGTACTGGTAAGACAACTTTGTCTACGGACCCGAAACGTTTGTTGATCGGTGACGATGAGCACGG
ATGGGATAACGAGGGTGTATTCAACTACGAGGGTGGTTGCTACGCTAAGGTTATCAACTTGGATAAAGAGAGCGAGCCGG
ATATCTACAACGCTATCAAACGTGACGCTCTTCTTGAGAACGTAACAGTTGCCGCTGATGGTAAGATCGATTTCGCTGAT
AAGTCAGTTACAGAGAACACTCGTGTTTCTTATCCGATCTATCACATCGAGAACATCGTTAAGCCGGTTTCTAAAGGCCC
GCACGCAAAACAAGTTATCTTCTTGTCTGCTGACGCATTTGGTGTATTGCCTCCAGTATCTATCTTGACTCCGGAGCAAA
CTCAATACTACTTCTTGTCTGGATTTACCGCTAAATTAGCAGGTACAGAGCGTGGTATCACGGAACCGACTCCTACATTC
TCCGCTTGTTTCGGTGCTGCTTTCTTGTCATTGCACCCGACAAAATACGGCGAAGAGTTGGTTAAGAAGATGGAAATGAC
TGGCGCTAAGGCTTACTTGGTGAACACAGGTTGGAACGGTAGCGGCAAACGTATCTCTATCAAAGATACAAGAGGTATCA
TCGACGCTATCCTTGATGGTTCTATCAATGAGGCTCCGACAAAGAAGATCCCGTATTTCGACTTCGAGGTTCCGACTGCA
TTACCGGGCGTAGATCCGAAGATCTTAGATCCTCGCGATACCTATGCTGATCCTGCTCAATGGGATGAGAAAGCGAAGGA
TTTGGCTGCTCGCTTCCAGAAGAACTTTGCTAAGTTCACGGGCAACGAAGCTGGTAAGGCATTGGTTGCTGCTGGTCCTC
AGTTATAA

Upstream 100 bases:

>100_bases
TGGAGTCGCAGCAAGCTCCAAAAGGTCGGAAAGCCTCACCGTATCGAACTAGAGTAGAGAGAAAAGTTATATAAAGTCAA
TCATTTAAATTAAATTCAAG

Downstream 100 bases:

>100_bases
TTGCTTAAAAAACCTTGATCAAAAAAGGTCAAGGTCTAAAAATATCTTTATAAAAAAGCGTGGAATTACTAATTCTGCGC
TTTTTTTATGATAACTACGT

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase [H]

Number of amino acids: Translated: 535; Mature: 534

Protein sequence:

>535_residues
MANLDLSKYGIVDVKEIVHNPSYDVLFAEETKPSLEGFEKGQVTELGAVNVMTGIYTGRSPKDKFFVKNEASEDSVWWTS
DEYKNDNKPCSEAAWADLKAKAVKELSGKRLFVVDTFCGANEATRLKVRFIMEVAWQAHFVTNMFIRPTAEELANYGEPD
FVSFNAAKAKVDNYKELGLNSETATVFNLKTKEQVILNTWYGGEMKKGIFSIMNYLNPLRGIASMHCSANTDKEGKSSAI
FFGLSGTGKTTLSTDPKRLLIGDDEHGWDNEGVFNYEGGCYAKVINLDKESEPDIYNAIKRDALLENVTVAADGKIDFAD
KSVTENTRVSYPIYHIENIVKPVSKGPHAKQVIFLSADAFGVLPPVSILTPEQTQYYFLSGFTAKLAGTERGITEPTPTF
SACFGAAFLSLHPTKYGEELVKKMEMTGAKAYLVNTGWNGSGKRISIKDTRGIIDAILDGSINEAPTKKIPYFDFEVPTA
LPGVDPKILDPRDTYADPAQWDEKAKDLAARFQKNFAKFTGNEAGKALVAAGPQL

Sequences:

>Translated_535_residues
MANLDLSKYGIVDVKEIVHNPSYDVLFAEETKPSLEGFEKGQVTELGAVNVMTGIYTGRSPKDKFFVKNEASEDSVWWTS
DEYKNDNKPCSEAAWADLKAKAVKELSGKRLFVVDTFCGANEATRLKVRFIMEVAWQAHFVTNMFIRPTAEELANYGEPD
FVSFNAAKAKVDNYKELGLNSETATVFNLKTKEQVILNTWYGGEMKKGIFSIMNYLNPLRGIASMHCSANTDKEGKSSAI
FFGLSGTGKTTLSTDPKRLLIGDDEHGWDNEGVFNYEGGCYAKVINLDKESEPDIYNAIKRDALLENVTVAADGKIDFAD
KSVTENTRVSYPIYHIENIVKPVSKGPHAKQVIFLSADAFGVLPPVSILTPEQTQYYFLSGFTAKLAGTERGITEPTPTF
SACFGAAFLSLHPTKYGEELVKKMEMTGAKAYLVNTGWNGSGKRISIKDTRGIIDAILDGSINEAPTKKIPYFDFEVPTA
LPGVDPKILDPRDTYADPAQWDEKAKDLAARFQKNFAKFTGNEAGKALVAAGPQL
>Mature_534_residues
ANLDLSKYGIVDVKEIVHNPSYDVLFAEETKPSLEGFEKGQVTELGAVNVMTGIYTGRSPKDKFFVKNEASEDSVWWTSD
EYKNDNKPCSEAAWADLKAKAVKELSGKRLFVVDTFCGANEATRLKVRFIMEVAWQAHFVTNMFIRPTAEELANYGEPDF
VSFNAAKAKVDNYKELGLNSETATVFNLKTKEQVILNTWYGGEMKKGIFSIMNYLNPLRGIASMHCSANTDKEGKSSAIF
FGLSGTGKTTLSTDPKRLLIGDDEHGWDNEGVFNYEGGCYAKVINLDKESEPDIYNAIKRDALLENVTVAADGKIDFADK
SVTENTRVSYPIYHIENIVKPVSKGPHAKQVIFLSADAFGVLPPVSILTPEQTQYYFLSGFTAKLAGTERGITEPTPTFS
ACFGAAFLSLHPTKYGEELVKKMEMTGAKAYLVNTGWNGSGKRISIKDTRGIIDAILDGSINEAPTKKIPYFDFEVPTAL
PGVDPKILDPRDTYADPAQWDEKAKDLAARFQKNFAKFTGNEAGKALVAAGPQL

Specific function: Rate-limiting gluconeogenic enzyme. [C]

COG id: COG1866

COG function: function code C; Phosphoenolpyruvate carboxykinase (ATP)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [ATP] family [H]

Homologues:

Organism=Escherichia coli, GI1789807, Length=532, Percent_Identity=69.3609022556391, Blast_Score=759, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6322950, Length=532, Percent_Identity=45.1127819548872, Blast_Score=452, Evalue=1e-128,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001272
- InterPro:   IPR013035
- InterPro:   IPR008210
- InterPro:   IPR015994 [H]

Pfam domain/function: PF01293 PEPCK_ATP [H]

EC number: =4.1.1.49 [H]

Molecular weight: Translated: 58960; Mature: 58829

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANLDLSKYGIVDVKEIVHNPSYDVLFAEETKPSLEGFEKGQVTELGAVNVMTGIYTGRS
CCCCCCCCCCCEEHHHHHCCCCCEEEEECCCCCCCCCCCCCCEEECCHHHHHHHEECCCC
PKDKFFVKNEASEDSVWWTSDEYKNDNKPCSEAAWADLKAKAVKELSGKRLFVVDTFCGA
CCCCEEEECCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCC
NEATRLKVRFIMEVAWQAHFVTNMFIRPTAEELANYGEPDFVSFNAAKAKVDNYKELGLN
CCCCEEEHHHHHHHHHHHHHHHHEEECCCHHHHHCCCCCCEEEECHHHHHHCCHHHCCCC
SETATVFNLKTKEQVILNTWYGGEMKKGIFSIMNYLNPLRGIASMHCSANTDKEGKSSAI
CCCEEEEEECCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEE
FFGLSGTGKTTLSTDPKRLLIGDDEHGWDNEGVFNYEGGCYAKVINLDKESEPDIYNAIK
EEEECCCCCCEECCCCCEEEECCCCCCCCCCCEEEECCCEEEEEEECCCCCCCHHHHHHH
RDALLENVTVAADGKIDFADKSVTENTRVSYPIYHIENIVKPVSKGPHAKQVIFLSADAF
HHHHHHCEEEEECCEEECCCCCCCCCCEEECCHHHHHHHHHHHHCCCCCEEEEEEECCCC
GVLPPVSILTPEQTQYYFLSGFTAKLAGTERGITEPTPTFSACFGAAFLSLHPTKYGEEL
CCCCCCEEECCCCCCEEEEECCEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHH
VKKMEMTGAKAYLVNTGWNGSGKRISIKDTRGIIDAILDGSINEAPTKKIPYFDFEVPTA
HHHHHHCCCEEEEEECCCCCCCCEEEEEHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCC
LPGVDPKILDPRDTYADPAQWDEKAKDLAARFQKNFAKFTGNEAGKALVAAGPQL
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCC
>Mature Secondary Structure 
ANLDLSKYGIVDVKEIVHNPSYDVLFAEETKPSLEGFEKGQVTELGAVNVMTGIYTGRS
CCCCCCCCCCEEHHHHHCCCCCEEEEECCCCCCCCCCCCCCEEECCHHHHHHHEECCCC
PKDKFFVKNEASEDSVWWTSDEYKNDNKPCSEAAWADLKAKAVKELSGKRLFVVDTFCGA
CCCCEEEECCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCC
NEATRLKVRFIMEVAWQAHFVTNMFIRPTAEELANYGEPDFVSFNAAKAKVDNYKELGLN
CCCCEEEHHHHHHHHHHHHHHHHEEECCCHHHHHCCCCCCEEEECHHHHHHCCHHHCCCC
SETATVFNLKTKEQVILNTWYGGEMKKGIFSIMNYLNPLRGIASMHCSANTDKEGKSSAI
CCCEEEEEECCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEE
FFGLSGTGKTTLSTDPKRLLIGDDEHGWDNEGVFNYEGGCYAKVINLDKESEPDIYNAIK
EEEECCCCCCEECCCCCEEEECCCCCCCCCCCEEEECCCEEEEEEECCCCCCCHHHHHHH
RDALLENVTVAADGKIDFADKSVTENTRVSYPIYHIENIVKPVSKGPHAKQVIFLSADAF
HHHHHHCEEEEECCEEECCCCCCCCCCEEECCHHHHHHHHHHHHCCCCCEEEEEEECCCC
GVLPPVSILTPEQTQYYFLSGFTAKLAGTERGITEPTPTFSACFGAAFLSLHPTKYGEEL
CCCCCCEEECCCCCCEEEEECCEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHH
VKKMEMTGAKAYLVNTGWNGSGKRISIKDTRGIIDAILDGSINEAPTKKIPYFDFEVPTA
HHHHHHCCCEEEEEECCCCCCCCEEEEEHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCC
LPGVDPKILDPRDTYADPAQWDEKAKDLAARFQKNFAKFTGNEAGKALVAAGPQL
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA