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The map label for this gene is metG

Identifier: 150009383

GI number: 150009383

Start: 3329074

End: 3331113

Strand: Reverse

Name: metG

Synonym: BDI_2791

Alternate gene names: 150009383

Gene position: 3331113-3329074 (Counterclockwise)

Preceding gene: 150009384

Following gene: 150009382

Centisome position: 69.23

GC content: 49.07

Gene sequence:

>2040_bases
ATGGAAAAACATTTTAAGAGAACGCTGATTACTACGGCGTTGCCGTATGCGAACGGACCGGTGCATATCGGCCATTTGGC
CGGGGTATATGTGCCTGCTGATATTTATGCCCGTTACCTTCGTTTGAAGGGGGAGGAAGTGTTGATGATTGGTGGTTCGG
ACGAACATGGGGTACCCATCACGTTGAGAGCGAAGAAAGAGGGGATCACACCGCAGGATGTGGTGGATCGTTACCACGGT
ATTATCAAGAAATCGTTCGAGGAGTTCGGTATCACGTTTGATATCTATTCTCGTACTACTTCCGCTACGCATCATCAGAT
GGCCTCGGATTTTTTCCGTACATTGTATGATAAAGGCGAATTCATCGAGAAAACAAGCGAGCAATATTACGATGAGGAGG
CGAAGCAATTCTTGGCCGACCGTTATATCATGGGTACTTGTCCGCATTGTGGAAACGAGAAGGCGTATGGCGATCAGTGC
GAGGCTTGCGGTACGTCTCTTAGCCCGACGGATTTGATCGATCCGAAATCCGCTATCAGTGGCAGCAAGCCGGTGATGCG
TGAGACGAAGCATTGGTATCTGCCTTTGGACAAGTGGGAGCCGTTTTTGCGTAAATGGATCTTGGAGGATCATAAGGAAT
GGAAACCGAACGTATATGGGCAGTGTAAGAGCTGGCTGGATATGGGACTGCAACCCCGTGCGGTGAGTCGTGATTTGGAT
TGGGGTATCCCTGTTCCCGTGGAAGGGGCGGAAGGAAAGGTGCTGTATGTCTGGTTCGACGCTCCGATCGGTTATATCTC
TAATACGAAAGAGTTGCTGCCGGATAGCTGGGAGACTTGGTGGAAGGATCCGGAGACTAAAATGGTCCATTTCATCGGTA
AAGATAATATCGTGTTCCATTGTATCGTGTTCCCTTCCATGCTGAAAGCGGAAGGTTCTTATAACTTGCCGGAGAATGTC
CCCGCTAATGAGTTCTTGAACTTGGAGGGCGACAAGATCTCTACTTCCCGCAACTGGGCGGTTTGGTTGAACGAGTATCT
GGTGGATATGCCGGGCAAGCAAGACGTGTTGCGCTATGTCTTGACGGCCAACGCGCCGGAGACAAAGGATAATGATTTCA
CATGGAAAGATTTTCAGGCTCGTAATAATAATGAGTTGGTCGCTATCTTAGGGAACTTCGTGAACCGTGCGTTAGTCCTT
ACGGATAAGTATTTCGAGGGTAAGGTGCCGGTGGCTGGCGAGTTGACAGATTATGATCGCCAGACCTTGAAGGATTTCGC
GGATGTGAAAGAGAACGTGGAACGCCTGTTGGATACCTACCACTTCCGTGACGCTCAGAAAGAGGCGATGAACCTAGCCC
GTATCGGAAATAAGTATCTCGCTGATATGGAGCCTTGGAAGTTGGCGAAGACGGATATGCCTCGTGTCGCTACGATCATG
AACATCGCTTTGCAGATTACGGCTAATCTGGCGATCGCTTTTGAGCCGTTCTTGCCGTTTAGCATGGAGAAACTGAATAA
GATGCTGAATGTAGAGCCGTTGGGATGGAATCGCCTAGGTGCTACGGATCTATTGGAGGCCGGTCATCAATTGGGCAAGG
CAGAGTTGTTGTTCGAGAAGATAGAGGATAGCGTGATCGAGGCCCAAGTGCAGAAATTATTGGATACGAAGAAAGCGAAT
GAGGAAGCGAATTATAAGGCGAAACCGATCCGTGAGAATATTGAGTTCGATGATTTCATGAAACTGGATATCCGTGTGGG
TACGGTTCTGGAATGTGTGAAGGTTCCGAAAGCGGATAAGCTGCTTCAGTTCCGTATCGACGATGGCCTGGAGAAACGCA
CGATCGTGTCGGGTATCGCCCAGCATTATAAACCCGAGGAATTGGTAGGCAAGCAAGTTTGCTTCATCGCTAACTTAGCT
CCCCGTAAGCTGAAAGGTATTGTTTCCGAGGGTATGATTCTCTCCGCCGAGAATTTCGATGGAAAGCTGGCCGTTATCAC
TCCGGAGAAAGAGGTGAAACCGGGTAGTGAGGTGAAATAA

Upstream 100 bases:

>100_bases
CTAGCCTCATTAAGAGGAAACTCACTCACAAAGGAAATTATTCTTTTTCGTCTAACCAAATTATTCCGTACCTTTGCCCC
TCAAAAAGAGAACACAAGGT

Downstream 100 bases:

>100_bases
TAAGATAAACGCAATAATGAGGGGGCTATTTCGTTTTAACGACGGGATAGCCCTATTTTTTGAATGGATAAGATGCTAGA
GAAACAGATACAGATGGTAG

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS

Number of amino acids: Translated: 679; Mature: 679

Protein sequence:

>679_residues
MEKHFKRTLITTALPYANGPVHIGHLAGVYVPADIYARYLRLKGEEVLMIGGSDEHGVPITLRAKKEGITPQDVVDRYHG
IIKKSFEEFGITFDIYSRTTSATHHQMASDFFRTLYDKGEFIEKTSEQYYDEEAKQFLADRYIMGTCPHCGNEKAYGDQC
EACGTSLSPTDLIDPKSAISGSKPVMRETKHWYLPLDKWEPFLRKWILEDHKEWKPNVYGQCKSWLDMGLQPRAVSRDLD
WGIPVPVEGAEGKVLYVWFDAPIGYISNTKELLPDSWETWWKDPETKMVHFIGKDNIVFHCIVFPSMLKAEGSYNLPENV
PANEFLNLEGDKISTSRNWAVWLNEYLVDMPGKQDVLRYVLTANAPETKDNDFTWKDFQARNNNELVAILGNFVNRALVL
TDKYFEGKVPVAGELTDYDRQTLKDFADVKENVERLLDTYHFRDAQKEAMNLARIGNKYLADMEPWKLAKTDMPRVATIM
NIALQITANLAIAFEPFLPFSMEKLNKMLNVEPLGWNRLGATDLLEAGHQLGKAELLFEKIEDSVIEAQVQKLLDTKKAN
EEANYKAKPIRENIEFDDFMKLDIRVGTVLECVKVPKADKLLQFRIDDGLEKRTIVSGIAQHYKPEELVGKQVCFIANLA
PRKLKGIVSEGMILSAENFDGKLAVITPEKEVKPGSEVK

Sequences:

>Translated_679_residues
MEKHFKRTLITTALPYANGPVHIGHLAGVYVPADIYARYLRLKGEEVLMIGGSDEHGVPITLRAKKEGITPQDVVDRYHG
IIKKSFEEFGITFDIYSRTTSATHHQMASDFFRTLYDKGEFIEKTSEQYYDEEAKQFLADRYIMGTCPHCGNEKAYGDQC
EACGTSLSPTDLIDPKSAISGSKPVMRETKHWYLPLDKWEPFLRKWILEDHKEWKPNVYGQCKSWLDMGLQPRAVSRDLD
WGIPVPVEGAEGKVLYVWFDAPIGYISNTKELLPDSWETWWKDPETKMVHFIGKDNIVFHCIVFPSMLKAEGSYNLPENV
PANEFLNLEGDKISTSRNWAVWLNEYLVDMPGKQDVLRYVLTANAPETKDNDFTWKDFQARNNNELVAILGNFVNRALVL
TDKYFEGKVPVAGELTDYDRQTLKDFADVKENVERLLDTYHFRDAQKEAMNLARIGNKYLADMEPWKLAKTDMPRVATIM
NIALQITANLAIAFEPFLPFSMEKLNKMLNVEPLGWNRLGATDLLEAGHQLGKAELLFEKIEDSVIEAQVQKLLDTKKAN
EEANYKAKPIRENIEFDDFMKLDIRVGTVLECVKVPKADKLLQFRIDDGLEKRTIVSGIAQHYKPEELVGKQVCFIANLA
PRKLKGIVSEGMILSAENFDGKLAVITPEKEVKPGSEVK
>Mature_679_residues
MEKHFKRTLITTALPYANGPVHIGHLAGVYVPADIYARYLRLKGEEVLMIGGSDEHGVPITLRAKKEGITPQDVVDRYHG
IIKKSFEEFGITFDIYSRTTSATHHQMASDFFRTLYDKGEFIEKTSEQYYDEEAKQFLADRYIMGTCPHCGNEKAYGDQC
EACGTSLSPTDLIDPKSAISGSKPVMRETKHWYLPLDKWEPFLRKWILEDHKEWKPNVYGQCKSWLDMGLQPRAVSRDLD
WGIPVPVEGAEGKVLYVWFDAPIGYISNTKELLPDSWETWWKDPETKMVHFIGKDNIVFHCIVFPSMLKAEGSYNLPENV
PANEFLNLEGDKISTSRNWAVWLNEYLVDMPGKQDVLRYVLTANAPETKDNDFTWKDFQARNNNELVAILGNFVNRALVL
TDKYFEGKVPVAGELTDYDRQTLKDFADVKENVERLLDTYHFRDAQKEAMNLARIGNKYLADMEPWKLAKTDMPRVATIM
NIALQITANLAIAFEPFLPFSMEKLNKMLNVEPLGWNRLGATDLLEAGHQLGKAELLFEKIEDSVIEAQVQKLLDTKKAN
EEANYKAKPIRENIEFDDFMKLDIRVGTVLECVKVPKADKLLQFRIDDGLEKRTIVSGIAQHYKPEELVGKQVCFIANLA
PRKLKGIVSEGMILSAENFDGKLAVITPEKEVKPGSEVK

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain

Homologues:

Organism=Homo sapiens, GI14043022, Length=610, Percent_Identity=37.7049180327869, Blast_Score=411, Evalue=1e-115,
Organism=Homo sapiens, GI24308436, Length=535, Percent_Identity=25.981308411215, Blast_Score=145, Evalue=9e-35,
Organism=Homo sapiens, GI4507947, Length=101, Percent_Identity=39.6039603960396, Blast_Score=68, Evalue=3e-11,
Organism=Escherichia coli, GI1788432, Length=698, Percent_Identity=34.9570200573066, Blast_Score=399, Evalue=1e-112,
Organism=Escherichia coli, GI1789455, Length=108, Percent_Identity=37.037037037037, Blast_Score=75, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17540794, Length=568, Percent_Identity=35.9154929577465, Blast_Score=400, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI212645096, Length=552, Percent_Identity=24.6376811594203, Blast_Score=124, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI71992781, Length=308, Percent_Identity=25.6493506493506, Blast_Score=81, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6321704, Length=549, Percent_Identity=36.6120218579235, Blast_Score=363, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24655311, Length=617, Percent_Identity=36.3047001620746, Blast_Score=392, Evalue=1e-109,
Organism=Drosophila melanogaster, GI21358483, Length=587, Percent_Identity=25.3833049403748, Blast_Score=125, Evalue=1e-28,
Organism=Drosophila melanogaster, GI21357223, Length=81, Percent_Identity=44.4444444444444, Blast_Score=70, Evalue=6e-12,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYM_PARD8 (A6LFP0)

Other databases:

- EMBL:   CP000140
- RefSeq:   YP_001304126.1
- ProteinModelPortal:   A6LFP0
- SMR:   A6LFP0
- STRING:   A6LFP0
- GeneID:   5307940
- GenomeReviews:   CP000140_GR
- KEGG:   pdi:BDI_2791
- eggNOG:   COG0143
- HOGENOM:   HBG721667
- OMA:   RASDHAY
- ProtClustDB:   PRK00133
- BioCyc:   PDIS435591:BDI_2791-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00098
- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01041
- TIGRFAMs:   TIGR00398
- TIGRFAMs:   TIGR00399

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind; SSF50249 Nucleic_acid_OB; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.10

Molecular weight: Translated: 77408; Mature: 77408

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50886 TRBD

Important sites: BINDING 335-335

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKHFKRTLITTALPYANGPVHIGHLAGVYVPADIYARYLRLKGEEVLMIGGSDEHGVPI
CCHHHHHHHHHHHCCCCCCCEEEEECCCEEECHHHHHHHHHCCCCEEEEECCCCCCCCCE
TLRAKKEGITPQDVVDRYHGIIKKSFEEFGITFDIYSRTTSATHHQMASDFFRTLYDKGE
EEEECCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHCCCH
FIEKTSEQYYDEEAKQFLADRYIMGTCPHCGNEKAYGDQCEACGTSLSPTDLIDPKSAIS
HHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCHHHHCCCCCCCCCCCCCHHHCC
GSKPVMRETKHWYLPLDKWEPFLRKWILEDHKEWKPNVYGQCKSWLDMGLQPRAVSRDLD
CCCCHHHCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHCCCCC
WGIPVPVEGAEGKVLYVWFDAPIGYISNTKELLPDSWETWWKDPETKMVHFIGKDNIVFH
CCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHCCCCHHHHCCCCCCEEEEEECCCCEEEE
CIVFPSMLKAEGSYNLPENVPANEFLNLEGDKISTSRNWAVWLNEYLVDMPGKQDVLRYV
EEECHHHHHCCCCCCCCCCCCHHHEECCCCCEECCCCCHHEEEHHHHHCCCCHHHHHHHH
LTANAPETKDNDFTWKDFQARNNNELVAILGNFVNRALVLTDKYFEGKVPVAGELTDYDR
HHCCCCCCCCCCCCCCHHCCCCCCCEEEEEHHHHCCEEEEEECCCCCCCCCCCCCCHHHH
QTLKDFADVKENVERLLDTYHFRDAQKEAMNLARIGNKYLADMEPWKLAKTDMPRVATIM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCEECCCCCCHHHHHH
NIALQITANLAIAFEPFLPFSMEKLNKMLNVEPLGWNRLGATDLLEAGHQLGKAELLFEK
HHHHHEEEEEEEEECCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHH
IEDSVIEAQVQKLLDTKKANEEANYKAKPIRENIEFDDFMKLDIRVGTVLECVKVPKADK
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHCCCCCCCEEEEEEEHHHHHHHHHCCCCCC
LLQFRIDDGLEKRTIVSGIAQHYKPEELVGKQVCFIANLAPRKLKGIVSEGMILSAENFD
EEEEECCCCCHHHHHHHHHHHHCCCHHHHCCCEEEHCCCCHHHHHHHHHCCEEEEECCCC
GKLAVITPEKEVKPGSEVK
CEEEEECCHHCCCCCCCCC
>Mature Secondary Structure
MEKHFKRTLITTALPYANGPVHIGHLAGVYVPADIYARYLRLKGEEVLMIGGSDEHGVPI
CCHHHHHHHHHHHCCCCCCCEEEEECCCEEECHHHHHHHHHCCCCEEEEECCCCCCCCCE
TLRAKKEGITPQDVVDRYHGIIKKSFEEFGITFDIYSRTTSATHHQMASDFFRTLYDKGE
EEEECCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHCCCH
FIEKTSEQYYDEEAKQFLADRYIMGTCPHCGNEKAYGDQCEACGTSLSPTDLIDPKSAIS
HHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCHHHHCCCCCCCCCCCCCHHHCC
GSKPVMRETKHWYLPLDKWEPFLRKWILEDHKEWKPNVYGQCKSWLDMGLQPRAVSRDLD
CCCCHHHCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHCCCCC
WGIPVPVEGAEGKVLYVWFDAPIGYISNTKELLPDSWETWWKDPETKMVHFIGKDNIVFH
CCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHCCCCHHHHCCCCCCEEEEEECCCCEEEE
CIVFPSMLKAEGSYNLPENVPANEFLNLEGDKISTSRNWAVWLNEYLVDMPGKQDVLRYV
EEECHHHHHCCCCCCCCCCCCHHHEECCCCCEECCCCCHHEEEHHHHHCCCCHHHHHHHH
LTANAPETKDNDFTWKDFQARNNNELVAILGNFVNRALVLTDKYFEGKVPVAGELTDYDR
HHCCCCCCCCCCCCCCHHCCCCCCCEEEEEHHHHCCEEEEEECCCCCCCCCCCCCCHHHH
QTLKDFADVKENVERLLDTYHFRDAQKEAMNLARIGNKYLADMEPWKLAKTDMPRVATIM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCEECCCCCCHHHHHH
NIALQITANLAIAFEPFLPFSMEKLNKMLNVEPLGWNRLGATDLLEAGHQLGKAELLFEK
HHHHHEEEEEEEEECCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHH
IEDSVIEAQVQKLLDTKKANEEANYKAKPIRENIEFDDFMKLDIRVGTVLECVKVPKADK
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHCCCCCCCEEEEEEEHHHHHHHHHCCCCCC
LLQFRIDDGLEKRTIVSGIAQHYKPEELVGKQVCFIANLAPRKLKGIVSEGMILSAENFD
EEEEECCCCCHHHHHHHHHHHHCCCHHHHCCCEEEHCCCCHHHHHHHHHCCEEEEECCCC
GKLAVITPEKEVKPGSEVK
CEEEEECCHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA