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The map label for this gene is pnp

Identifier: 150009352

GI number: 150009352

Start: 3287851

End: 3290091

Strand: Direct

Name: pnp

Synonym: BDI_2760

Alternate gene names: 150009352

Gene position: 3287851-3290091 (Clockwise)

Preceding gene: 150009348

Following gene: 150009353

Centisome position: 68.33

GC content: 49.58

Gene sequence:

>2241_bases
ATGCTTAATCCAATTAACAAGACGATCGAGTTAGGTGATGGAAGAACCATTACCATCGAAACCGGGAAATTGGCCAAACA
GGCGGACGGAGCGGTTACCGTTCGTATGAACAACACCGTGTTGCTCGCCACTGTTTGTGCCGCTAAAGACGCGAACCCGG
GTGTTGACTTCATGCCGTTGCAAGTAGAATACAAAGAGAAATACTCCGCTTTTGGACGTTTCCCCGGAGGTTTTACAAAA
AGAGAAGGAAAAGCTTCTGATTATGAAATCCTCACATCTCGTTTAGTAGACCGTGCGTTGCGTCCTCTTTTCCCAGACAA
CTACCACGCTGAGGTATATGTAAATATTATATTGTTCTCCGCTGATGGCGAAGATTTGCCGGACGCATTAGCAGGTTTGG
CCGCTTCCGCCGCTTTGGCAGTTTCTGATATTCCTTTTAACGGACCGATCTCTGAGGTTCGCGTAGCTCGTACGGATGGA
AAATACATCGTCAACCCAACTTCCGCAGAATTGGAGAAAGCTGATATCGACATCATGGTTGCCGCTACCATAGACAACAT
CATGATGGTTGAGGGTGAAATGAACGAGGTTCAGGAATCTGAGATGCTTGAGGCTATCAAGGTCGCTCACGAGGCTATCA
AGGTACAATGCAAGGCTCAATTGGAATTATCCGAGGCTTGCGGCAAATTGGTAAAACGTGAATATTGCCACGAGGTAAAC
GATGACGAGTTGCGCAAGGACGTACACGACAAATGTTACGCCAAGGCTTACGCCGTAGCCACATCTGGTAGCGGCAAGCA
CGAACGCAGCGAGGCATTCGAGAAAATCGTAGAAGAATACAAGGCTCAATTCTCCGAGGAGGAGTTGACGGACGAGAAAC
TGGAAATGATCGGTCGCTACTATCACGATGTAGAGAAAGAGGCTATGCGCCGTGCCATCCTTGATGAGGGTAAGCGTTTG
GATGGTCGTAAGACTACCGAGATCCGTCCGATCTGGATCGAGACAGATTGCCTGCCGGGTCCTCACGGTTCCGCTATCTT
TACACGTGGAGAGACACAGTCTTTATCTACGGTAACTTTAGGTACGAAGTCTGATGAGAAAATGATAGACGACGTATTGA
ACCATGGATACGAGCGTTTCCTTTTACATTATAACTTCCCCCCATTCTCCACAGGCGAGGCCAAAGCAACACGTGGCGTA
GGCCGTCGTGAGATCGGACACGGCAACTTGGCTCACCGTGCGCTGAAACGTATGATCCCGGACAACTATCCGTATGTAGT
ACGTGTAATCTCCGATATCTTGGAGTCTAACGGTTCTTCTTCTATGGCTACGGTTTGCGCCGGTACCTTGGCGTTGAGAG
ACGCAGGTGTCCCGATGAAGAAACCGGTATCCGGTATCGCTATGGGATTGATCTCCGAGAACAAGGGTACGAACTACGCG
ATCTTGTCTGATATCTTAGGCGACGAGGATCACTTGGGTGATATGGACTTCAAGGTAACGGGTACGAAAGACGGTATCAC
GGCAACTCAGATGGATATCAAGGTAGACGGTTTGTCTTACGAGATCCTTGAGAATGCTTTGGCTCAAGCTAAAGAGGGTC
GTATGCATATCTTGGGCAAGATATTGGAGGCTCAACCGGAAGCTCGTGAGGATTTGAAACCTCATGCACCTCGTATCGAG
ACGATGATTATCGGTAAGGAATTCATCGGTGCGGTAATCGGCCCGGGCGGTAAGATCATCCAAGGTATCCAAGAGAAAAC
GGGAGCTACGGTATCTATCGATGAGGTAGACGGCGTAGGAAAGATCGAGATCTCCGGCACGAATAAGGCTACGATCGACG
CGGCTGTCAAGGCGATCAAGGCTATCGTCGCTGTTCCTGAGATCGGAGAGGTTTATGAAGGAAAGATTTCCTCTATCATG
CCTTATGGTGCATTCGTTGAGTTCATGCCGGGTAAGGATGGTTTGCTTCACATTTCCGAGATCGATTGGAAACGTCTGGA
GACCGTTGAGCAAGCCGGCTTGAAGGAAGGCGATACGGTTTCTGTCAAGTTGGTTGATATCGATCCGAAGACAGGCAAGT
TCAAACTTTCCCGCAAGGTATTGCTTCCGAAACCGGAAGGTTACGAGGAACGTCCGCCAAGACCAGAAAGAGGTGAGCGT
GGACCTCGCCAAGATCGTGGAGACCGTGGTCCTCGTCAGGATCGTGGTGATCGCGGACCTCGCAGGGAGTACAGAGACTA
A

Upstream 100 bases:

>100_bases
CTTTAAGACTGTTATTTCTTTTCATTTTCATGCAATTACAGGTAAACTTTCATTACATTTGCACCAATTTTTGACAGATA
GAAAAATAAGAAAAAAGATT

Downstream 100 bases:

>100_bases
GATAAGGTCTTATACATTATATATAATCCCGGGTAGATTAAACACTATCCGGGATTTTTTTATCAAATCATCTACCACAC
CTACAACTATAGCTATAATC

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 746; Mature: 746

Protein sequence:

>746_residues
MLNPINKTIELGDGRTITIETGKLAKQADGAVTVRMNNTVLLATVCAAKDANPGVDFMPLQVEYKEKYSAFGRFPGGFTK
REGKASDYEILTSRLVDRALRPLFPDNYHAEVYVNIILFSADGEDLPDALAGLAASAALAVSDIPFNGPISEVRVARTDG
KYIVNPTSAELEKADIDIMVAATIDNIMMVEGEMNEVQESEMLEAIKVAHEAIKVQCKAQLELSEACGKLVKREYCHEVN
DDELRKDVHDKCYAKAYAVATSGSGKHERSEAFEKIVEEYKAQFSEEELTDEKLEMIGRYYHDVEKEAMRRAILDEGKRL
DGRKTTEIRPIWIETDCLPGPHGSAIFTRGETQSLSTVTLGTKSDEKMIDDVLNHGYERFLLHYNFPPFSTGEAKATRGV
GRREIGHGNLAHRALKRMIPDNYPYVVRVISDILESNGSSSMATVCAGTLALRDAGVPMKKPVSGIAMGLISENKGTNYA
ILSDILGDEDHLGDMDFKVTGTKDGITATQMDIKVDGLSYEILENALAQAKEGRMHILGKILEAQPEAREDLKPHAPRIE
TMIIGKEFIGAVIGPGGKIIQGIQEKTGATVSIDEVDGVGKIEISGTNKATIDAAVKAIKAIVAVPEIGEVYEGKISSIM
PYGAFVEFMPGKDGLLHISEIDWKRLETVEQAGLKEGDTVSVKLVDIDPKTGKFKLSRKVLLPKPEGYEERPPRPERGER
GPRQDRGDRGPRQDRGDRGPRREYRD

Sequences:

>Translated_746_residues
MLNPINKTIELGDGRTITIETGKLAKQADGAVTVRMNNTVLLATVCAAKDANPGVDFMPLQVEYKEKYSAFGRFPGGFTK
REGKASDYEILTSRLVDRALRPLFPDNYHAEVYVNIILFSADGEDLPDALAGLAASAALAVSDIPFNGPISEVRVARTDG
KYIVNPTSAELEKADIDIMVAATIDNIMMVEGEMNEVQESEMLEAIKVAHEAIKVQCKAQLELSEACGKLVKREYCHEVN
DDELRKDVHDKCYAKAYAVATSGSGKHERSEAFEKIVEEYKAQFSEEELTDEKLEMIGRYYHDVEKEAMRRAILDEGKRL
DGRKTTEIRPIWIETDCLPGPHGSAIFTRGETQSLSTVTLGTKSDEKMIDDVLNHGYERFLLHYNFPPFSTGEAKATRGV
GRREIGHGNLAHRALKRMIPDNYPYVVRVISDILESNGSSSMATVCAGTLALRDAGVPMKKPVSGIAMGLISENKGTNYA
ILSDILGDEDHLGDMDFKVTGTKDGITATQMDIKVDGLSYEILENALAQAKEGRMHILGKILEAQPEAREDLKPHAPRIE
TMIIGKEFIGAVIGPGGKIIQGIQEKTGATVSIDEVDGVGKIEISGTNKATIDAAVKAIKAIVAVPEIGEVYEGKISSIM
PYGAFVEFMPGKDGLLHISEIDWKRLETVEQAGLKEGDTVSVKLVDIDPKTGKFKLSRKVLLPKPEGYEERPPRPERGER
GPRQDRGDRGPRQDRGDRGPRREYRD
>Mature_746_residues
MLNPINKTIELGDGRTITIETGKLAKQADGAVTVRMNNTVLLATVCAAKDANPGVDFMPLQVEYKEKYSAFGRFPGGFTK
REGKASDYEILTSRLVDRALRPLFPDNYHAEVYVNIILFSADGEDLPDALAGLAASAALAVSDIPFNGPISEVRVARTDG
KYIVNPTSAELEKADIDIMVAATIDNIMMVEGEMNEVQESEMLEAIKVAHEAIKVQCKAQLELSEACGKLVKREYCHEVN
DDELRKDVHDKCYAKAYAVATSGSGKHERSEAFEKIVEEYKAQFSEEELTDEKLEMIGRYYHDVEKEAMRRAILDEGKRL
DGRKTTEIRPIWIETDCLPGPHGSAIFTRGETQSLSTVTLGTKSDEKMIDDVLNHGYERFLLHYNFPPFSTGEAKATRGV
GRREIGHGNLAHRALKRMIPDNYPYVVRVISDILESNGSSSMATVCAGTLALRDAGVPMKKPVSGIAMGLISENKGTNYA
ILSDILGDEDHLGDMDFKVTGTKDGITATQMDIKVDGLSYEILENALAQAKEGRMHILGKILEAQPEAREDLKPHAPRIE
TMIIGKEFIGAVIGPGGKIIQGIQEKTGATVSIDEVDGVGKIEISGTNKATIDAAVKAIKAIVAVPEIGEVYEGKISSIM
PYGAFVEFMPGKDGLLHISEIDWKRLETVEQAGLKEGDTVSVKLVDIDPKTGKFKLSRKVLLPKPEGYEERPPRPERGER
GPRQDRGDRGPRQDRGDRGPRREYRD

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=715, Percent_Identity=37.2027972027972, Blast_Score=429, Evalue=1e-120,
Organism=Escherichia coli, GI145693187, Length=705, Percent_Identity=44.3971631205674, Blast_Score=555, Evalue=1e-159,
Organism=Caenorhabditis elegans, GI115534063, Length=707, Percent_Identity=35.0777934936351, Blast_Score=366, Evalue=1e-101,
Organism=Drosophila melanogaster, GI281362905, Length=718, Percent_Identity=36.7688022284123, Blast_Score=435, Evalue=1e-122,
Organism=Drosophila melanogaster, GI24651641, Length=718, Percent_Identity=36.7688022284123, Blast_Score=435, Evalue=1e-122,
Organism=Drosophila melanogaster, GI24651643, Length=718, Percent_Identity=36.7688022284123, Blast_Score=435, Evalue=1e-122,
Organism=Drosophila melanogaster, GI161079377, Length=655, Percent_Identity=36.793893129771, Blast_Score=386, Evalue=1e-107,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_PARD8 (A6LFK9)

Other databases:

- EMBL:   CP000140
- RefSeq:   YP_001304095.1
- ProteinModelPortal:   A6LFK9
- SMR:   A6LFK9
- STRING:   A6LFK9
- GeneID:   5307909
- GenomeReviews:   CP000140_GR
- KEGG:   pdi:BDI_2760
- eggNOG:   COG1185
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- ProtClustDB:   PRK11824
- BioCyc:   PDIS435591:BDI_2760-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 82020; Mature: 82020

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNPINKTIELGDGRTITIETGKLAKQADGAVTVRMNNTVLLATVCAAKDANPGVDFMPL
CCCCCCCEEECCCCCEEEEECCCCHHCCCCEEEEEECCEEEEEEEECCCCCCCCCCEEEE
QVEYKEKYSAFGRFPGGFTKREGKASDYEILTSRLVDRALRPLFPDNYHAEVYVNIILFS
EEEHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEE
ADGEDLPDALAGLAASAALAVSDIPFNGPISEVRVARTDGKYIVNPTSAELEKADIDIMV
CCCCCHHHHHHHHHHHHHHEEECCCCCCCHHHEEEEECCCCEEECCCCCHHHHCCCCEEE
AATIDNIMMVEGEMNEVQESEMLEAIKVAHEAIKVQCKAQLELSEACGKLVKREYCHEVN
EEECCCEEEEECCHHHHHHHHHHHHHHHHHHHHEEHHHHHEHHHHHHHHHHHHHHHCCCC
DDELRKDVHDKCYAKAYAVATSGSGKHERSEAFEKIVEEYKAQFSEEELTDEKLEMIGRY
HHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHH
YHDVEKEAMRRAILDEGKRLDGRKTTEIRPIWIETDCLPGPHGSAIFTRGETQSLSTVTL
HHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEECCCCCCCCCEEEECCCCCCEEEEEE
GTKSDEKMIDDVLNHGYERFLLHYNFPPFSTGEAKATRGVGRREIGHGNLAHRALKRMIP
CCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHCC
DNYPYVVRVISDILESNGSSSMATVCAGTLALRDAGVPMKKPVSGIAMGLISENKGTNYA
CCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHEEEEECCCCCCEE
ILSDILGDEDHLGDMDFKVTGTKDGITATQMDIKVDGLSYEILENALAQAKEGRMHILGK
HHHHHCCCCCCCCCCCEEEECCCCCCEEEEEEEEECCCHHHHHHHHHHHHHCCHHHHHHH
ILEAQPEAREDLKPHAPRIETMIIGKEFIGAVIGPGGKIIQGIQEKTGATVSIDEVDGVG
HHHCCCCHHHCCCCCCCCEEEEEECHHHHHHEECCCHHHHHHHHHHCCCEEEEECCCCCE
KIEISGTNKATIDAAVKAIKAIVAVPEIGEVYEGKISSIMPYGAFVEFMPGKDGLLHISE
EEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHCCHHHHCCCCCCEEEECCCCCCEEEEEC
IDWKRLETVEQAGLKEGDTVSVKLVDIDPKTGKFKLSRKVLLPKPEGYEERPPRPERGER
CCHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCEEEECEEECCCCCCCCCCCCCCCCCCC
GPRQDRGDRGPRQDRGDRGPRREYRD
CCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLNPINKTIELGDGRTITIETGKLAKQADGAVTVRMNNTVLLATVCAAKDANPGVDFMPL
CCCCCCCEEECCCCCEEEEECCCCHHCCCCEEEEEECCEEEEEEEECCCCCCCCCCEEEE
QVEYKEKYSAFGRFPGGFTKREGKASDYEILTSRLVDRALRPLFPDNYHAEVYVNIILFS
EEEHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEE
ADGEDLPDALAGLAASAALAVSDIPFNGPISEVRVARTDGKYIVNPTSAELEKADIDIMV
CCCCCHHHHHHHHHHHHHHEEECCCCCCCHHHEEEEECCCCEEECCCCCHHHHCCCCEEE
AATIDNIMMVEGEMNEVQESEMLEAIKVAHEAIKVQCKAQLELSEACGKLVKREYCHEVN
EEECCCEEEEECCHHHHHHHHHHHHHHHHHHHHEEHHHHHEHHHHHHHHHHHHHHHCCCC
DDELRKDVHDKCYAKAYAVATSGSGKHERSEAFEKIVEEYKAQFSEEELTDEKLEMIGRY
HHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHH
YHDVEKEAMRRAILDEGKRLDGRKTTEIRPIWIETDCLPGPHGSAIFTRGETQSLSTVTL
HHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEECCCCCCCCCEEEECCCCCCEEEEEE
GTKSDEKMIDDVLNHGYERFLLHYNFPPFSTGEAKATRGVGRREIGHGNLAHRALKRMIP
CCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHCC
DNYPYVVRVISDILESNGSSSMATVCAGTLALRDAGVPMKKPVSGIAMGLISENKGTNYA
CCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHEEEEECCCCCCEE
ILSDILGDEDHLGDMDFKVTGTKDGITATQMDIKVDGLSYEILENALAQAKEGRMHILGK
HHHHHCCCCCCCCCCCEEEECCCCCCEEEEEEEEECCCHHHHHHHHHHHHHCCHHHHHHH
ILEAQPEAREDLKPHAPRIETMIIGKEFIGAVIGPGGKIIQGIQEKTGATVSIDEVDGVG
HHHCCCCHHHCCCCCCCCEEEEEECHHHHHHEECCCHHHHHHHHHHCCCEEEEECCCCCE
KIEISGTNKATIDAAVKAIKAIVAVPEIGEVYEGKISSIMPYGAFVEFMPGKDGLLHISE
EEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHCCHHHHCCCCCCEEEECCCCCCEEEEEC
IDWKRLETVEQAGLKEGDTVSVKLVDIDPKTGKFKLSRKVLLPKPEGYEERPPRPERGER
CCHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCEEEECEEECCCCCCCCCCCCCCCCCCC
GPRQDRGDRGPRQDRGDRGPRREYRD
CCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA