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The map label for this gene is prfB [H]

Identifier: 150009345

GI number: 150009345

Start: 3280538

End: 3281506

Strand: Reverse

Name: prfB [H]

Synonym: BDI_2753

Alternate gene names: 150009345

Gene position: 3281506-3280538 (Counterclockwise)

Preceding gene: 150009346

Following gene: 150009341

Centisome position: 68.2

GC content: 50.15

Gene sequence:

>969_bases
ATGAAGAAAGTAAAGGAGCTAAAGAAATGGATCGAGCTTTACGGTGAGGTACATGCGGCGGCTGAGGAGCTAGAGTTGGC
TTACGAATATGTAAAGGAAGAGATTATCACCGAGGAAGAGGTGGACGCGGCTTATAAGAAGGCCCTTCATTTACTGGAAG
ACTTGGAGTTCCGCAATATGCTTCGCGACGAGGCTGATCAAATGAGCTGCGTGCTCAAGATCAACTCGGGTGCCGGTGGT
ACCGAGAGCCAAGATTGGGCTTCCATGTTGATGCGTATGTATCAGCGTTGGGCTGAGAATAACGGTTATAAGATCTCTAT
CGCCAACTATCAGGAAGGGGATGAGGCCGGTATCAAGACCGTGACCTTCAATATTGAGGGCGATTATGCCTATGGCTATC
TAAAGAGCGAGAATGGCGTGCATCGTTTGGTACGTGTATCTCCTTACAACGCCCAAGGTAAACGAATGACCTCTTTCGCC
TCCGTATTCGTCACCCCGTTGGTGGATGATACGATCGAGGTAAAGGTAGAGACGGCGGCTATCTCTTGGGATACGTTCCG
TTCGGGCGGCGCCGGAGGACAGAACGTGAACAAGGTGGAATCCGGAGTTCGCTTGCGTTATCAGTTCAAGGACCCGTATA
CAGGTGAGGAAGAAGAGATCTTGATCGAGAATACCGAGACTCGCGACCAACCCAAGAACCGTGAGAACGCTATGCGTCAA
TTGCGTTCCATCCTTTACGATAAGGAGTTGCAGCATCGCATGGCCGAGCAGGCCAAGGTGGAGGCCGGAAAGAAAAAGAT
CGAGTGGGGTTCGCAGATCCGTAGTTACGTATTCGATGACCGTCGCGTAAAAGACCATCGTACGAACTACCAGACTTCCG
ACGTGAACGGTGTGATGGATGGAAAGATCGACGATTTCATCAAAGCATACTTGATGGAGTTCGCCGGAGAAGAAAGTGCG
GAAAAATAA

Upstream 100 bases:

>100_bases
TATCTTTGACATCGATAGCAAGACCATTCAGTTAGAAGAGGAGGAGCTACGTACGCAAGACCCCGGTTTCTGGGAAGACG
CCAAACGTGCGGAAGTCCAG

Downstream 100 bases:

>100_bases
GTTTTTCCCTTTAACATTTGCATATCTCGATAATAAATCGTAATATTGCACCCGATTTCAGAGCCTAGCTCTGAACAGGT
CCTATAGCTCAGTTGGTTAG

Product: peptide chain release factor RF-2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MKKVKELKKWIELYGEVHAAAEELELAYEYVKEEIITEEEVDAAYKKALHLLEDLEFRNMLRDEADQMSCVLKINSGAGG
TESQDWASMLMRMYQRWAENNGYKISIANYQEGDEAGIKTVTFNIEGDYAYGYLKSENGVHRLVRVSPYNAQGKRMTSFA
SVFVTPLVDDTIEVKVETAAISWDTFRSGGAGGQNVNKVESGVRLRYQFKDPYTGEEEEILIENTETRDQPKNRENAMRQ
LRSILYDKELQHRMAEQAKVEAGKKKIEWGSQIRSYVFDDRRVKDHRTNYQTSDVNGVMDGKIDDFIKAYLMEFAGEESA
EK

Sequences:

>Translated_322_residues
MKKVKELKKWIELYGEVHAAAEELELAYEYVKEEIITEEEVDAAYKKALHLLEDLEFRNMLRDEADQMSCVLKINSGAGG
TESQDWASMLMRMYQRWAENNGYKISIANYQEGDEAGIKTVTFNIEGDYAYGYLKSENGVHRLVRVSPYNAQGKRMTSFA
SVFVTPLVDDTIEVKVETAAISWDTFRSGGAGGQNVNKVESGVRLRYQFKDPYTGEEEEILIENTETRDQPKNRENAMRQ
LRSILYDKELQHRMAEQAKVEAGKKKIEWGSQIRSYVFDDRRVKDHRTNYQTSDVNGVMDGKIDDFIKAYLMEFAGEESA
EK
>Mature_322_residues
MKKVKELKKWIELYGEVHAAAEELELAYEYVKEEIITEEEVDAAYKKALHLLEDLEFRNMLRDEADQMSCVLKINSGAGG
TESQDWASMLMRMYQRWAENNGYKISIANYQEGDEAGIKTVTFNIEGDYAYGYLKSENGVHRLVRVSPYNAQGKRMTSFA
SVFVTPLVDDTIEVKVETAAISWDTFRSGGAGGQNVNKVESGVRLRYQFKDPYTGEEEEILIENTETRDQPKNRENAMRQ
LRSILYDKELQHRMAEQAKVEAGKKKIEWGSQIRSYVFDDRRVKDHRTNYQTSDVNGVMDGKIDDFIKAYLMEFAGEESA
EK

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=291, Percent_Identity=30.5841924398625, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI34577120, Length=271, Percent_Identity=32.1033210332103, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI166795305, Length=176, Percent_Identity=28.9772727272727, Blast_Score=86, Evalue=3e-17,
Organism=Escherichia coli, GI2367172, Length=290, Percent_Identity=48.9655172413793, Blast_Score=270, Evalue=1e-73,
Organism=Escherichia coli, GI1787462, Length=264, Percent_Identity=34.0909090909091, Blast_Score=144, Evalue=7e-36,
Organism=Caenorhabditis elegans, GI17542784, Length=253, Percent_Identity=30.4347826086957, Blast_Score=93, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6321295, Length=255, Percent_Identity=30.1960784313725, Blast_Score=96, Evalue=8e-21,
Organism=Drosophila melanogaster, GI19921226, Length=290, Percent_Identity=33.7931034482759, Blast_Score=115, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 37050; Mature: 37050

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVKELKKWIELYGEVHAAAEELELAYEYVKEEIITEEEVDAAYKKALHLLEDLEFRNM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
LRDEADQMSCVLKINSGAGGTESQDWASMLMRMYQRWAENNGYKISIANYQEGDEAGIKT
HHCCHHHEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEE
VTFNIEGDYAYGYLKSENGVHRLVRVSPYNAQGKRMTSFASVFVTPLVDDTIEVKVETAA
EEEEECCCEEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEEE
ISWDTFRSGGAGGQNVNKVESGVRLRYQFKDPYTGEEEEILIENTETRDQPKNRENAMRQ
EEHHHCCCCCCCCCCHHHHHCCCEEEEEECCCCCCCCCEEEEECCCCCCCCCCHHHHHHH
LRSILYDKELQHRMAEQAKVEAGKKKIEWGSQIRSYVFDDRRVKDHRTNYQTSDVNGVMD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCC
GKIDDFIKAYLMEFAGEESAEK
CCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKKVKELKKWIELYGEVHAAAEELELAYEYVKEEIITEEEVDAAYKKALHLLEDLEFRNM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
LRDEADQMSCVLKINSGAGGTESQDWASMLMRMYQRWAENNGYKISIANYQEGDEAGIKT
HHCCHHHEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEE
VTFNIEGDYAYGYLKSENGVHRLVRVSPYNAQGKRMTSFASVFVTPLVDDTIEVKVETAA
EEEEECCCEEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEEE
ISWDTFRSGGAGGQNVNKVESGVRLRYQFKDPYTGEEEEILIENTETRDQPKNRENAMRQ
EEHHHCCCCCCCCCCHHHHHCCCEEEEEECCCCCCCCCEEEEECCCCCCCCCCHHHHHHH
LRSILYDKELQHRMAEQAKVEAGKKKIEWGSQIRSYVFDDRRVKDHRTNYQTSDVNGVMD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCC
GKIDDFIKAYLMEFAGEESAEK
CCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]