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The map label for this gene is mvaIM [H]

Identifier: 150009310

GI number: 150009310

Start: 3220765

End: 3222009

Strand: Reverse

Name: mvaIM [H]

Synonym: BDI_2712

Alternate gene names: 150009310

Gene position: 3222009-3220765 (Counterclockwise)

Preceding gene: 150009313

Following gene: 150009309

Centisome position: 66.97

GC content: 35.9

Gene sequence:

>1245_bases
ATGTACAAAAGAACGATTGATAATAGGTGGAGCTTTAAGGATGCCGATACCAAAGAATTTACTCATTGCTATCATGCGTA
TCCTGCGATGATGATTCCCCAAGTAGCAAGAGCTCTAATAGAGGAATATAGGCCAGAGGATGGAGTTGAGCTACTTTTTG
ATCCATATATGGGAAGCGGTACATCGTTAGTTGAGGCTTCTATAAAAGGTATAAACGCGATAGGAACAGACCTTAATCCA
TTGGCTAGATTGATGAGCCATGTAAAAACAACCCATTATGATTTATCTTGTATCAGAGATACTTTTTCAATGATGCAAGC
CTTGTTCTTTGAGTATTCTGAAGATAAGGTCAAGAATAAGAATTTCGATAATATTTCTAATTATACTTATTGGTATTCCA
GAGATAGCCTTTTGCGCCTTTCTTATATTTATCAAGTAATTAATGAATGTGTGGCTTTGGATTTCGCTGATTTCTTTAAA
GTTCCTCTTTCCGAGACTGTTAGAGAGGTGTCGTTTACGAGGAATGGAGAATTTAAGCGGTTTAGGATGAAGGAAGAGAA
AATTAAAGACTTCAAACCGGATGTCTTTCGATTGTTTGAAGAAAAGGTTATAAGAAATATAAATGGACTTGAGGAGTTTA
ATAGTATAAAATATCTATGTAATATAGGTATCTATGACTTTAATAGTACTATAGAAATCCCTTCCGATATAATACAGCCG
AATAGTGTTGATATGGTTGTAACCTCTCCGCCTTATGGCGATAGTAGAACAACCGTTGCTTATGGACAATTTTCAAGGTG
GGCAAATGAATGGTTCAATTTTGAAAATGCTAAAACACTAGATAATCTCCTAATGGGGGGACGAGTTCAGAAAGAGGAAC
TTTTTGAGACTAAAAGCATTAAATCTGAACTGGACGAAATAAAGAGCATTGACCCAAAGAGAAATTTGGAAGTGGTCTCG
TTTCTTAATGATTATAGCTGTTCTATAAAGAATGTCGCGTCTGTAATAAAGAGTGGTGGAGTCGTTTGTTATGTCGTAGG
GAACAGAACGGTTAAGGGGGTTCAAATTCCATTAGATTACTTTACTGCTGAAATGTTTGAGAAGAATGGTTTTACACATA
TCGATACTATTGTTAGAGAAATACCTAACAAAAGGATGCCTTCCAAAACAAGTCCTACTAATGTGGCAGGGAAGAAGGTT
AGCACGATGTGCAATGAATATATAGTCATATTGGAGAAGAAATAA

Upstream 100 bases:

>100_bases
GTTATTTTTGCCCAAAAATATAGTTTTATATATGCTTATGTACTCACAATATGTGAGCAATTGAGTATATTTGCACTCAC
ATAATATAAGTAAGGAAGGT

Downstream 100 bases:

>100_bases
GGAAAGAGGCTGTTTTATTTGACTGAATTGATAGCCAATGGCTCCTAGTGTCGAGTTCTTTCTTTTTGACAGTTTGTAAT
CTTAACGCCCGTAGAATCGA

Product: adenine-specific DNA methylase

Products: NA

Alternate protein names: M.MvaI; N-4 cytosine-specific methyltransferase MvaI [H]

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MYKRTIDNRWSFKDADTKEFTHCYHAYPAMMIPQVARALIEEYRPEDGVELLFDPYMGSGTSLVEASIKGINAIGTDLNP
LARLMSHVKTTHYDLSCIRDTFSMMQALFFEYSEDKVKNKNFDNISNYTYWYSRDSLLRLSYIYQVINECVALDFADFFK
VPLSETVREVSFTRNGEFKRFRMKEEKIKDFKPDVFRLFEEKVIRNINGLEEFNSIKYLCNIGIYDFNSTIEIPSDIIQP
NSVDMVVTSPPYGDSRTTVAYGQFSRWANEWFNFENAKTLDNLLMGGRVQKEELFETKSIKSELDEIKSIDPKRNLEVVS
FLNDYSCSIKNVASVIKSGGVVCYVVGNRTVKGVQIPLDYFTAEMFEKNGFTHIDTIVREIPNKRMPSKTSPTNVAGKKV
STMCNEYIVILEKK

Sequences:

>Translated_414_residues
MYKRTIDNRWSFKDADTKEFTHCYHAYPAMMIPQVARALIEEYRPEDGVELLFDPYMGSGTSLVEASIKGINAIGTDLNP
LARLMSHVKTTHYDLSCIRDTFSMMQALFFEYSEDKVKNKNFDNISNYTYWYSRDSLLRLSYIYQVINECVALDFADFFK
VPLSETVREVSFTRNGEFKRFRMKEEKIKDFKPDVFRLFEEKVIRNINGLEEFNSIKYLCNIGIYDFNSTIEIPSDIIQP
NSVDMVVTSPPYGDSRTTVAYGQFSRWANEWFNFENAKTLDNLLMGGRVQKEELFETKSIKSELDEIKSIDPKRNLEVVS
FLNDYSCSIKNVASVIKSGGVVCYVVGNRTVKGVQIPLDYFTAEMFEKNGFTHIDTIVREIPNKRMPSKTSPTNVAGKKV
STMCNEYIVILEKK
>Mature_414_residues
MYKRTIDNRWSFKDADTKEFTHCYHAYPAMMIPQVARALIEEYRPEDGVELLFDPYMGSGTSLVEASIKGINAIGTDLNP
LARLMSHVKTTHYDLSCIRDTFSMMQALFFEYSEDKVKNKNFDNISNYTYWYSRDSLLRLSYIYQVINECVALDFADFFK
VPLSETVREVSFTRNGEFKRFRMKEEKIKDFKPDVFRLFEEKVIRNINGLEEFNSIKYLCNIGIYDFNSTIEIPSDIIQP
NSVDMVVTSPPYGDSRTTVAYGQFSRWANEWFNFENAKTLDNLLMGGRVQKEELFETKSIKSELDEIKSIDPKRNLEVVS
FLNDYSCSIKNVASVIKSGGVVCYVVGNRTVKGVQIPLDYFTAEMFEKNGFTHIDTIVREIPNKRMPSKTSPTNVAGKKV
STMCNEYIVILEKK

Specific function: This methylase recognizes the double-stranded sequence CCWGG, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the MvaI endonuclease [H]

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017985
- InterPro:   IPR000241 [H]

Pfam domain/function: PF01170 UPF0020 [H]

EC number: =2.1.1.113 [H]

Molecular weight: Translated: 47860; Mature: 47860

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS00093 N4_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYKRTIDNRWSFKDADTKEFTHCYHAYPAMMIPQVARALIEEYRPEDGVELLFDPYMGSG
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCC
TSLVEASIKGINAIGTDLNPLARLMSHVKTTHYDLSCIRDTFSMMQALFFEYSEDKVKNK
CHHHHHHHCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCC
NFDNISNYTYWYSRDSLLRLSYIYQVINECVALDFADFFKVPLSETVREVSFTRNGEFKR
CCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHH
FRMKEEKIKDFKPDVFRLFEEKVIRNINGLEEFNSIKYLCNIGIYDFNSTIEIPSDIIQP
HHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEECCCCCCCCHHHCCC
NSVDMVVTSPPYGDSRTTVAYGQFSRWANEWFNFENAKTLDNLLMGGRVQKEELFETKSI
CCCEEEEECCCCCCCCCEEEHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHH
KSELDEIKSIDPKRNLEVVSFLNDYSCSIKNVASVIKSGGVVCYVVGNRTVKGVQIPLDY
HHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEECCCEEEEEECCHHH
FTAEMFEKNGFTHIDTIVREIPNKRMPSKTSPTNVAGKKVSTMCNEYIVILEKK
HHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCEEEEEECC
>Mature Secondary Structure
MYKRTIDNRWSFKDADTKEFTHCYHAYPAMMIPQVARALIEEYRPEDGVELLFDPYMGSG
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCC
TSLVEASIKGINAIGTDLNPLARLMSHVKTTHYDLSCIRDTFSMMQALFFEYSEDKVKNK
CHHHHHHHCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCC
NFDNISNYTYWYSRDSLLRLSYIYQVINECVALDFADFFKVPLSETVREVSFTRNGEFKR
CCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHH
FRMKEEKIKDFKPDVFRLFEEKVIRNINGLEEFNSIKYLCNIGIYDFNSTIEIPSDIIQP
HHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEECCCCCCCCHHHCCC
NSVDMVVTSPPYGDSRTTVAYGQFSRWANEWFNFENAKTLDNLLMGGRVQKEELFETKSI
CCCEEEEECCCCCCCCCEEEHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHH
KSELDEIKSIDPKRNLEVVSFLNDYSCSIKNVASVIKSGGVVCYVVGNRTVKGVQIPLDY
HHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEECCCEEEEEECCHHH
FTAEMFEKNGFTHIDTIVREIPNKRMPSKTSPTNVAGKKVSTMCNEYIVILEKK
HHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2690010 [H]