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The map label for this gene is cobB [H]

Identifier: 150009308

GI number: 150009308

Start: 3218903

End: 3219592

Strand: Direct

Name: cobB [H]

Synonym: BDI_2710

Alternate gene names: 150009308

Gene position: 3218903-3219592 (Clockwise)

Preceding gene: 150009307

Following gene: 150009311

Centisome position: 66.9

GC content: 50.72

Gene sequence:

>690_bases
ATGAAAAAGTTAGTCATACTTACCGGAGCGGGAATGAGCGCGGAAAGCGGCATCTCCACTTTCCGTGACTCGGACGGGTT
ATGGGAGAAATATCGTGTAGAAGAGGTAGCTACACCAGAAGGCTTCGCCGCTAACCCAAAATTGGTTCTCGACTTCTACA
ACCAGCGTAGAAGAGAGCTATTGAATACGAAACCTAACGCCGGTCACCTCGGCCTAGCCGCCTTGGAGAAAGATTTCGAC
GTACGGATCATTACCCAGAATATCGATAACTTACATGAACGTGCGGGTAGCAGCCACGTGATCCATCTGCATGGAGAGTT
GATGAAGGCATGCTCCGTAAGGGATCTGGACACGACCTACGATCTATCACCGGAGAACCCGGATATTCATCTGGGTGACA
AGGACCCTCACGGAAATCAGCTACGTCCTTTCGTCGTATGGTTTGGAGAGGCGGTACCCATGATAGAGCCTTCCATCAAG
CTGGTGGAAGATTGTGATATCTTCGTGATTATCGGTACCTCCTTAAACGTCTATCCTGCCGCCGGATTGCTAAACTACGT
GCGCCGGGGCCAACCGATCTACTTGATCGACCCGAAAGATGTACGTACTTATCGCAACGATATTGAGCATATACAGGCCG
GGGCCTCGGAAGGGGTCAAGAGGCTGACCGAGTTGCTTACGCAGAATTAA

Upstream 100 bases:

>100_bases
AAGAAAGCGATGAAATGTAATTATTCATCGCTTTTTCAAGATAAAGTCGTAACTTCGTAACTATTAATATATACATCTAT
ACATTTTAAACAAGTAACAG

Downstream 100 bases:

>100_bases
ACAATTCATACACTCATTAGGTCTGACGAAGGCAAGTATAAAAACGAAGTCCTCGGCATGCGAACACATTGCCAAGGACT
TGTTTTTTTATCCGCTTCCA

Product: NAD-dependent deacetylase

Products: NA

Alternate protein names: Regulatory protein SIR2 homolog [H]

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MKKLVILTGAGMSAESGISTFRDSDGLWEKYRVEEVATPEGFAANPKLVLDFYNQRRRELLNTKPNAGHLGLAALEKDFD
VRIITQNIDNLHERAGSSHVIHLHGELMKACSVRDLDTTYDLSPENPDIHLGDKDPHGNQLRPFVVWFGEAVPMIEPSIK
LVEDCDIFVIIGTSLNVYPAAGLLNYVRRGQPIYLIDPKDVRTYRNDIEHIQAGASEGVKRLTELLTQN

Sequences:

>Translated_229_residues
MKKLVILTGAGMSAESGISTFRDSDGLWEKYRVEEVATPEGFAANPKLVLDFYNQRRRELLNTKPNAGHLGLAALEKDFD
VRIITQNIDNLHERAGSSHVIHLHGELMKACSVRDLDTTYDLSPENPDIHLGDKDPHGNQLRPFVVWFGEAVPMIEPSIK
LVEDCDIFVIIGTSLNVYPAAGLLNYVRRGQPIYLIDPKDVRTYRNDIEHIQAGASEGVKRLTELLTQN
>Mature_229_residues
MKKLVILTGAGMSAESGISTFRDSDGLWEKYRVEEVATPEGFAANPKLVLDFYNQRRRELLNTKPNAGHLGLAALEKDFD
VRIITQNIDNLHERAGSSHVIHLHGELMKACSVRDLDTTYDLSPENPDIHLGDKDPHGNQLRPFVVWFGEAVPMIEPSIK
LVEDCDIFVIIGTSLNVYPAAGLLNYVRRGQPIYLIDPKDVRTYRNDIEHIQAGASEGVKRLTELLTQN

Specific function: Modulates the activities of several enzymes which are inactive in their acetylated form [H]

COG id: COG0846

COG function: function code K; NAD-dependent protein deacetylases, SIR2 family

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 deacetylase sirtuin-type domain [H]

Homologues:

Organism=Homo sapiens, GI300795542, Length=203, Percent_Identity=39.4088669950739, Blast_Score=140, Evalue=9e-34,
Organism=Homo sapiens, GI13787215, Length=221, Percent_Identity=37.10407239819, Blast_Score=135, Evalue=3e-32,
Organism=Homo sapiens, GI6912664, Length=221, Percent_Identity=37.10407239819, Blast_Score=135, Evalue=3e-32,
Organism=Homo sapiens, GI7657575, Length=232, Percent_Identity=27.5862068965517, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI308199517, Length=193, Percent_Identity=51.2953367875648, Blast_Score=177, Evalue=4e-46,
Organism=Caenorhabditis elegans, GI17541892, Length=226, Percent_Identity=30.0884955752212, Blast_Score=77, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17567771, Length=238, Percent_Identity=26.890756302521, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003000 [H]

Pfam domain/function: PF02146 SIR2 [H]

EC number: 3.5.1.- [C]

Molecular weight: Translated: 25645; Mature: 25645

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: PS50305 SIRTUIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLVILTGAGMSAESGISTFRDSDGLWEKYRVEEVATPEGFAANPKLVLDFYNQRRREL
CCEEEEEECCCCCCCCCCHHHCCCCCCHHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHH
LNTKPNAGHLGLAALEKDFDVRIITQNIDNLHERAGSSHVIHLHGELMKACSVRDLDTTY
HCCCCCCCCCCHHHHCCCCCEEEEECCHHHHHHHCCCCCEEEECHHHHHHCCCCCCCCEE
DLSPENPDIHLGDKDPHGNQLRPFVVWFGEAVPMIEPSIKLVEDCDIFVIIGTSLNVYPA
CCCCCCCCEEECCCCCCCCCCCEEEEEECCCCCCCCCCCEEECCCCEEEEECCCCCEECH
AGLLNYVRRGQPIYLIDPKDVRTYRNDIEHIQAGASEGVKRLTELLTQN
HHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKLVILTGAGMSAESGISTFRDSDGLWEKYRVEEVATPEGFAANPKLVLDFYNQRRREL
CCEEEEEECCCCCCCCCCHHHCCCCCCHHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHH
LNTKPNAGHLGLAALEKDFDVRIITQNIDNLHERAGSSHVIHLHGELMKACSVRDLDTTY
HCCCCCCCCCCHHHHCCCCCEEEEECCHHHHHHHCCCCCEEEECHHHHHHCCCCCCCCEE
DLSPENPDIHLGDKDPHGNQLRPFVVWFGEAVPMIEPSIKLVEDCDIFVIIGTSLNVYPA
CCCCCCCCEEECCCCCCCCCCCEEEEEECCCCCCCCCCCEEECCCCEEEEECCCCCEECH
AGLLNYVRRGQPIYLIDPKDVRTYRNDIEHIQAGASEGVKRLTELLTQN
HHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12663928 [H]