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The map label for this gene is folE

Identifier: 150009291

GI number: 150009291

Start: 3200763

End: 3201353

Strand: Reverse

Name: folE

Synonym: BDI_2693

Alternate gene names: 150009291

Gene position: 3201353-3200763 (Counterclockwise)

Preceding gene: 150009292

Following gene: 150009288

Centisome position: 66.54

GC content: 49.58

Gene sequence:

>591_bases
ATGTCAGAAGTAAACGAAGAGACGCTACGTGCCCGGGAAGCTTTGGCCGGCCATTATAAGGAAGTATTGGGTTTGCTGGG
TGAGGACGTGGAGCGTGAGGGTCTGCTTAAAACGCCGGAACGTGTAGCGAAAGCGATGCAGTTTTTAACCAAGGGATACC
ATGAGGATCCGGAGGCGGTACTCCGTTCCGCCATGTTTCAGGAAGAGGATTACAAACAGATGGTTATCGTGAAAGATATC
GATTTCTTCTCCCTTTGTGAGCATCATATGTTGCCTTTCTTTGGCAAGGCCCACGTGGCTTATATCCCCAAGAAATATAT
TACGGGCTTAAGCAAGATCCCTAGGGTAGTGGATATCTTCGCCCGCCGCCTTCAGATACAGGAGCGTATGACGATGCAGA
TCAAGGATTGCATCCAGAGGACCTTGGACCCGTTGGGCGTGATGGTCGTGATCGAGGCGCAGCATATGTGTATGCAGATG
CGTGGCGTGGAGAAACAAAATTCCTTGACGACTACCTCTGATTTCACAGGCTTTTTCCAACAAGCTAAGACGCGCGAGGA
GTTTATGAATCTGATCAAGCATAACCGCTGA

Upstream 100 bases:

>100_bases
CCTATCATATGATGCGCCTGTTGATGGGTGCTTTCCCGAAATACGGGAAGGAAATGTATATCGTACGAGAAGAGATAAAA
ATTCCCCTAAATTAAAGAGA

Downstream 100 bases:

>100_bases
TGACTTAATACAAGTCGGCATGCGTACCAGTATCGGTCATGATCAAGACCAGTTCGGTATCGTTTTGTTGCCATATTAAG
AGCCAGTCGCTTTTAATATG

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I

Number of amino acids: Translated: 196; Mature: 195

Protein sequence:

>196_residues
MSEVNEETLRAREALAGHYKEVLGLLGEDVEREGLLKTPERVAKAMQFLTKGYHEDPEAVLRSAMFQEEDYKQMVIVKDI
DFFSLCEHHMLPFFGKAHVAYIPKKYITGLSKIPRVVDIFARRLQIQERMTMQIKDCIQRTLDPLGVMVVIEAQHMCMQM
RGVEKQNSLTTTSDFTGFFQQAKTREEFMNLIKHNR

Sequences:

>Translated_196_residues
MSEVNEETLRAREALAGHYKEVLGLLGEDVEREGLLKTPERVAKAMQFLTKGYHEDPEAVLRSAMFQEEDYKQMVIVKDI
DFFSLCEHHMLPFFGKAHVAYIPKKYITGLSKIPRVVDIFARRLQIQERMTMQIKDCIQRTLDPLGVMVVIEAQHMCMQM
RGVEKQNSLTTTSDFTGFFQQAKTREEFMNLIKHNR
>Mature_195_residues
SEVNEETLRAREALAGHYKEVLGLLGEDVEREGLLKTPERVAKAMQFLTKGYHEDPEAVLRSAMFQEEDYKQMVIVKDID
FFSLCEHHMLPFFGKAHVAYIPKKYITGLSKIPRVVDIFARRLQIQERMTMQIKDCIQRTLDPLGVMVVIEAQHMCMQMR
GVEKQNSLTTTSDFTGFFQQAKTREEFMNLIKHNR

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family

Homologues:

Organism=Homo sapiens, GI66932968, Length=180, Percent_Identity=57.2222222222222, Blast_Score=215, Evalue=3e-56,
Organism=Homo sapiens, GI4503949, Length=180, Percent_Identity=57.2222222222222, Blast_Score=215, Evalue=3e-56,
Organism=Homo sapiens, GI66932972, Length=139, Percent_Identity=56.8345323741007, Blast_Score=169, Evalue=1e-42,
Organism=Homo sapiens, GI66932970, Length=139, Percent_Identity=56.8345323741007, Blast_Score=169, Evalue=1e-42,
Organism=Escherichia coli, GI1788476, Length=197, Percent_Identity=39.0862944162437, Blast_Score=124, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI17560486, Length=182, Percent_Identity=54.3956043956044, Blast_Score=209, Evalue=6e-55,
Organism=Saccharomyces cerevisiae, GI6321706, Length=179, Percent_Identity=54.1899441340782, Blast_Score=202, Evalue=2e-53,
Organism=Drosophila melanogaster, GI24656782, Length=187, Percent_Identity=54.0106951871658, Blast_Score=194, Evalue=4e-50,
Organism=Drosophila melanogaster, GI24656791, Length=187, Percent_Identity=54.0106951871658, Blast_Score=193, Evalue=5e-50,
Organism=Drosophila melanogaster, GI24656796, Length=187, Percent_Identity=54.0106951871658, Blast_Score=193, Evalue=7e-50,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): GCH1_PARD8 (A6LFE8)

Other databases:

- EMBL:   CP000140
- RefSeq:   YP_001304034.1
- ProteinModelPortal:   A6LFE8
- SMR:   A6LFE8
- STRING:   A6LFE8
- GeneID:   5307842
- GenomeReviews:   CP000140_GR
- KEGG:   pdi:BDI_2693
- eggNOG:   COG0302
- HOGENOM:   HBG370191
- OMA:   ARIVEMF
- ProtClustDB:   PRK09347
- BioCyc:   PDIS435591:BDI_2693-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00223
- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234
- TIGRFAMs:   TIGR00063

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =3.5.4.16

Molecular weight: Translated: 22802; Mature: 22671

Theoretical pI: Translated: 7.20; Mature: 7.20

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1; PS00860 GTP_CYCLOHYDROL_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
6.1 %Met     (Translated Protein)
7.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
7.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEVNEETLRAREALAGHYKEVLGLLGEDVEREGLLKTPERVAKAMQFLTKGYHEDPEAV
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHH
LRSAMFQEEDYKQMVIVKDIDFFSLCEHHMLPFFGKAHVAYIPKKYITGLSKIPRVVDIF
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHCCHHHHHCCHHHHHHHHHHHHHHHHH
ARRLQIQERMTMQIKDCIQRTLDPLGVMVVIEAQHMCMQMRGVEKQNSLTTTSDFTGFFQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCHHHHHHHHH
QAKTREEFMNLIKHNR
HHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SEVNEETLRAREALAGHYKEVLGLLGEDVEREGLLKTPERVAKAMQFLTKGYHEDPEAV
CCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHH
LRSAMFQEEDYKQMVIVKDIDFFSLCEHHMLPFFGKAHVAYIPKKYITGLSKIPRVVDIF
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHCCHHHHHCCHHHHHHHHHHHHHHHHH
ARRLQIQERMTMQIKDCIQRTLDPLGVMVVIEAQHMCMQMRGVEKQNSLTTTSDFTGFFQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCHHHHHHHHH
QAKTREEFMNLIKHNR
HHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA