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The map label for this gene is birA [H]

Identifier: 150009161

GI number: 150009161

Start: 3037331

End: 3038083

Strand: Reverse

Name: birA [H]

Synonym: BDI_2563

Alternate gene names: 150009161

Gene position: 3038083-3037331 (Counterclockwise)

Preceding gene: 150009162

Following gene: 150009160

Centisome position: 63.14

GC content: 45.82

Gene sequence:

>753_bases
ATGATGAGCACTGATAATGAATCTCCCCGCATCTTGCGTGTGGCGGAAACCGCTTCTACGAATAGCTTGCTTCGTGAGTT
GGTCATCAAGGAATCTCTTGCGGAAGGAAGTGTGGTCGTTGCCGATTTCCAGACAGCCGGACGTGGTCAGATCGGGAATA
CTTGGGAGTCTGAGGCGGGAAAGAACTTGATGTTTAGCTTAGTCCTGTACCCTACGTGCATCCCTGCGAACCGTCAGTTC
TTGATTTCTCAAATAGCAGCTCTAAGCGTGAAGGAGGCGTTGGACTTATATGCGGATCATATCACCGTGAAGTGGCCGAA
CGATATTTACTGGAAAGACAAGAAGATTTGCGGAATGTTGATTGAGAACGACCTGTCGGGGCATAATCTGTATTGCTCCA
TCATCGGGATCGGCATAAACTTGAATCAAACGGTATTTCGAGGAGATGCTCCGAATCCTGTATCTTTGTTTCAAATAATC
GGTAAGGAAGTAGATCGGGAAGAGGTTTTGCACCGTTTCCTCTCAATATTCTATCGCTATTATTTATCTCTTCTCCAAGA
GGAACACGAGGATATTCGAGCTCGTTATCAATCCGCTTTGTATAGAAGAGAGGGGTATCATGGTTATAAGGATGAGTCCG
GCGAGTTCGAGGCCTGTATCCATGATATAGAATCAACCGGGCATCTTTTGCTAGCATTACGGGATGGAAGCATTCGCCGT
TATGCTTTTAAGGAAGTCTCTTATTGCAAATGA

Upstream 100 bases:

>100_bases
CTGGACCGGGATATGGAAGACGAGGAGATTAAACGCTGGATCTTCCATTCTTACCGGGAAGTGATCGCTAAACTACCAAA
AAATATAAGGGAAATTTATA

Downstream 100 bases:

>100_bases
CTTTTTGATCGGAGTGGTTTTTTACCACGTATTTTTATAATGTACACGTACATTGTAAAATGTGTGTAATAGTGTATATC
TGTTGGAGGAATGAACCGAA

Product: putative biotin-(acetyl-CoA carboxylase) synthetase

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MMSTDNESPRILRVAETASTNSLLRELVIKESLAEGSVVVADFQTAGRGQIGNTWESEAGKNLMFSLVLYPTCIPANRQF
LISQIAALSVKEALDLYADHITVKWPNDIYWKDKKICGMLIENDLSGHNLYCSIIGIGINLNQTVFRGDAPNPVSLFQII
GKEVDREEVLHRFLSIFYRYYLSLLQEEHEDIRARYQSALYRREGYHGYKDESGEFEACIHDIESTGHLLLALRDGSIRR
YAFKEVSYCK

Sequences:

>Translated_250_residues
MMSTDNESPRILRVAETASTNSLLRELVIKESLAEGSVVVADFQTAGRGQIGNTWESEAGKNLMFSLVLYPTCIPANRQF
LISQIAALSVKEALDLYADHITVKWPNDIYWKDKKICGMLIENDLSGHNLYCSIIGIGINLNQTVFRGDAPNPVSLFQII
GKEVDREEVLHRFLSIFYRYYLSLLQEEHEDIRARYQSALYRREGYHGYKDESGEFEACIHDIESTGHLLLALRDGSIRR
YAFKEVSYCK
>Mature_250_residues
MMSTDNESPRILRVAETASTNSLLRELVIKESLAEGSVVVADFQTAGRGQIGNTWESEAGKNLMFSLVLYPTCIPANRQF
LISQIAALSVKEALDLYADHITVKWPNDIYWKDKKICGMLIENDLSGHNLYCSIIGIGINLNQTVFRGDAPNPVSLFQII
GKEVDREEVLHRFLSIFYRYYLSLLQEEHEDIRARYQSALYRREGYHGYKDESGEFEACIHDIESTGHLLLALRDGSIRR
YAFKEVSYCK

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Homo sapiens, GI46255045, Length=144, Percent_Identity=34.0277777777778, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1790408, Length=138, Percent_Identity=34.7826086956522, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 28496; Mature: 28496

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSTDNESPRILRVAETASTNSLLRELVIKESLAEGSVVVADFQTAGRGQIGNTWESEAG
CCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHCC
KNLMFSLVLYPTCIPANRQFLISQIAALSVKEALDLYADHITVKWPNDIYWKDKKICGML
CHHEEHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCEECCHHHHHHH
IENDLSGHNLYCSIIGIGINLNQTVFRGDAPNPVSLFQIIGKEVDREEVLHRFLSIFYRY
HHCCCCCCEEEEEEEEECCCCCCEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHH
YLSLLQEEHEDIRARYQSALYRREGYHGYKDESGEFEACIHDIESTGHLLLALRDGSIRR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCEEEEEEECCCCHH
YAFKEVSYCK
HHHHHHCCCC
>Mature Secondary Structure
MMSTDNESPRILRVAETASTNSLLRELVIKESLAEGSVVVADFQTAGRGQIGNTWESEAG
CCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHCC
KNLMFSLVLYPTCIPANRQFLISQIAALSVKEALDLYADHITVKWPNDIYWKDKKICGML
CHHEEHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCEECCHHHHHHH
IENDLSGHNLYCSIIGIGINLNQTVFRGDAPNPVSLFQIIGKEVDREEVLHRFLSIFYRY
HHCCCCCCEEEEEEEEECCCCCCEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHH
YLSLLQEEHEDIRARYQSALYRREGYHGYKDESGEFEACIHDIESTGHLLLALRDGSIRR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCEEEEEEECCCCHH
YAFKEVSYCK
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7730294 [H]