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The map label for this gene is mltD [H]

Identifier: 150009154

GI number: 150009154

Start: 3028028

End: 3029584

Strand: Reverse

Name: mltD [H]

Synonym: BDI_2556

Alternate gene names: 150009154

Gene position: 3029584-3028028 (Counterclockwise)

Preceding gene: 150009155

Following gene: 150009153

Centisome position: 62.97

GC content: 46.63

Gene sequence:

>1557_bases
ATGAAAAGATACCTGCTAGCACTCATTTGTTTAAATTGTTTGGTCTCATTGGTCTATGCGCAGGAAGAGACCCGAATTGA
GGAAAAAGATACCTTAAGCTCGCTGGAGTCTGAGGTCGGATTGATTCCGGAAAGCTTGGATGCGAACGTGGATAGTTTGC
TCCGTACTTGGCATGTGCAATATTTCTCAAAAAGAGAGGACTTTTGCCATGATGACGACGAGAATGTCTATTTCCCGGAT
TCGGTCTATATCGACCGCCTGTCTAGGCTTCCCAGCGTGATCGCTTTGCCCTATAATAATATAGTGCGTGATTGTATTGA
CTTATATGCGGAGCGTAAACGAAATTTAGTGCGTTATATGCTGGGGATGGCGGACTTTTATTTTCCGATAATCGAGCAGG
TGTTGGATGAGCATGGCCTGCCTATTGAATTGAAATATCTCGCCGTCGTGGAAAGTGCCTTGAATCCGGTAGCCCTCTCT
CGTGTGGGCGCTTGTGGTTTATGGCAATTCATGCTTCCTACGGGTAAGAGCTATGGCTTGGAGATTAATAGCTTGGTGGA
CGAGCGCCGGGACCCCTTGAAAGCGACCGAGGCCGCTTGTAAGTATTTCAAGGATATGTACGCTATCTATGGGGATTGGA
ATTTAGTGTTGGCATCTTATAACTGCGGGCCGGGCAACGTGAATAAAGCGATCCGTCGTTCGGGGGGCAAGACGGACTTC
TGGGATATCTTCCCTTATTTACCGAAAGAGACTCGTTCATACGTCCCCTTATTTATTGCCGCCAATTACGTCATGAATTA
TTATTGCGAGCATAACATCTGTCCGATGCAAACCAGCTTGCCGCTAGCCACGGATACGGTCATGGTGAATAATGCCCTAC
ATTTGCAGCAGGTATCGGATCTATTGCAAGTAGATATTGAGACGTTGCGTGCGTTAAATCCACAATATAAAAGAGACATC
GTTCCGGGTAATACCCGCCCTTCGGTTTTGAAACTACCTGCGGCGGAAACCTATGCTTTTATCGATAAAGAAGATACGGT
CTATACGCATCGGATCGAGGAACTGTTGGCGAATTGCATTTCTGCGAATAACTCTAATGGCTCTTTACCAAGTGGTGCGA
CCCGGGAAAAAATCACCCACGTGGTATTGAGTGGTGAGAATCTTTATACGATCGCTAATCGCTATGGCGTGACAGCGAAA
GATATCCGTAAATGGAACGGGCTAGGCTCCAACCGGGTAGCGAAAGGAAAACGGTTAAGATTATATGTAGATAATGGGGG
TGTCGCTTTTGCCTCGGCTACCAAGACTCCGGTGAAATCAACTACCGTGACTGCGAAATCGACTGCGACAACAACGAAAA
AGTCGGCTTCCGCCGCTACAAAACAGATCGCTTCCGCTAATAAAGATTTTGTCTCTTATAAGGTTAAATCGGGAGATTCG
CTTTATTCGATTTCCAAGAAATACCCTGGGGTTACCGCCTCTTCCTTACAAAAGGTGAATGGGCTGTCAAGTCCGGATAT
CCGTCCGGGTCAAGTACTGAAGATACCGGTAGGATGA

Upstream 100 bases:

>100_bases
CACCGGATTTATCCTTGCATTTGGAGCCGGTTGTTAGTCCGAAGACGAGTGTCACGCCTCGTACTTACGGATTGAATTGC
AGTCTAAAATTCTGATATAC

Downstream 100 bases:

>100_bases
TCATTCGGGGATAATTCGTCTTTAACTCTTTTTTTTCGCTTTCTTCCTGCACTGGAGAAGGATGTGAATGATTTTTTATT
CCTTTGTATACGAAAAAAGA

Product: glycoside hydrolase family protein

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: Murein hydrolase D; Regulatory protein dniR [H]

Number of amino acids: Translated: 518; Mature: 518

Protein sequence:

>518_residues
MKRYLLALICLNCLVSLVYAQEETRIEEKDTLSSLESEVGLIPESLDANVDSLLRTWHVQYFSKREDFCHDDDENVYFPD
SVYIDRLSRLPSVIALPYNNIVRDCIDLYAERKRNLVRYMLGMADFYFPIIEQVLDEHGLPIELKYLAVVESALNPVALS
RVGACGLWQFMLPTGKSYGLEINSLVDERRDPLKATEAACKYFKDMYAIYGDWNLVLASYNCGPGNVNKAIRRSGGKTDF
WDIFPYLPKETRSYVPLFIAANYVMNYYCEHNICPMQTSLPLATDTVMVNNALHLQQVSDLLQVDIETLRALNPQYKRDI
VPGNTRPSVLKLPAAETYAFIDKEDTVYTHRIEELLANCISANNSNGSLPSGATREKITHVVLSGENLYTIANRYGVTAK
DIRKWNGLGSNRVAKGKRLRLYVDNGGVAFASATKTPVKSTTVTAKSTATTTKKSASAATKQIASANKDFVSYKVKSGDS
LYSISKKYPGVTASSLQKVNGLSSPDIRPGQVLKIPVG

Sequences:

>Translated_518_residues
MKRYLLALICLNCLVSLVYAQEETRIEEKDTLSSLESEVGLIPESLDANVDSLLRTWHVQYFSKREDFCHDDDENVYFPD
SVYIDRLSRLPSVIALPYNNIVRDCIDLYAERKRNLVRYMLGMADFYFPIIEQVLDEHGLPIELKYLAVVESALNPVALS
RVGACGLWQFMLPTGKSYGLEINSLVDERRDPLKATEAACKYFKDMYAIYGDWNLVLASYNCGPGNVNKAIRRSGGKTDF
WDIFPYLPKETRSYVPLFIAANYVMNYYCEHNICPMQTSLPLATDTVMVNNALHLQQVSDLLQVDIETLRALNPQYKRDI
VPGNTRPSVLKLPAAETYAFIDKEDTVYTHRIEELLANCISANNSNGSLPSGATREKITHVVLSGENLYTIANRYGVTAK
DIRKWNGLGSNRVAKGKRLRLYVDNGGVAFASATKTPVKSTTVTAKSTATTTKKSASAATKQIASANKDFVSYKVKSGDS
LYSISKKYPGVTASSLQKVNGLSSPDIRPGQVLKIPVG
>Mature_518_residues
MKRYLLALICLNCLVSLVYAQEETRIEEKDTLSSLESEVGLIPESLDANVDSLLRTWHVQYFSKREDFCHDDDENVYFPD
SVYIDRLSRLPSVIALPYNNIVRDCIDLYAERKRNLVRYMLGMADFYFPIIEQVLDEHGLPIELKYLAVVESALNPVALS
RVGACGLWQFMLPTGKSYGLEINSLVDERRDPLKATEAACKYFKDMYAIYGDWNLVLASYNCGPGNVNKAIRRSGGKTDF
WDIFPYLPKETRSYVPLFIAANYVMNYYCEHNICPMQTSLPLATDTVMVNNALHLQQVSDLLQVDIETLRALNPQYKRDI
VPGNTRPSVLKLPAAETYAFIDKEDTVYTHRIEELLANCISANNSNGSLPSGATREKITHVVLSGENLYTIANRYGVTAK
DIRKWNGLGSNRVAKGKRLRLYVDNGGVAFASATKTPVKSTTVTAKSTATTTKKSASAATKQIASANKDFVSYKVKSGDS
LYSISKKYPGVTASSLQKVNGLSSPDIRPGQVLKIPVG

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1786405, Length=428, Percent_Identity=27.5700934579439, Blast_Score=154, Evalue=2e-38,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR010511
- InterPro:   IPR018392
- InterPro:   IPR002482
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01476 LysM; PF06474 MLTD_N; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 57796; Mature: 57796

Theoretical pI: Translated: 8.21; Mature: 8.21

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRYLLALICLNCLVSLVYAQEETRIEEKDTLSSLESEVGLIPESLDANVDSLLRTWHVQ
CHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
YFSKREDFCHDDDENVYFPDSVYIDRLSRLPSVIALPYNNIVRDCIDLYAERKRNLVRYM
HHHHCCCCCCCCCCCEECCCHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHH
LGMADFYFPIIEQVLDEHGLPIELKYLAVVESALNPVALSRVGACGLWQFMLPTGKSYGL
HHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHCCHHHHHHHCCHHHHHHHCCCCCCCCE
EINSLVDERRDPLKATEAACKYFKDMYAIYGDWNLVLASYNCGPGNVNKAIRRSGGKTDF
EHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHCCCCCCH
WDIFPYLPKETRSYVPLFIAANYVMNYYCEHNICPMQTSLPLATDTVMVNNALHLQQVSD
HHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEECEEEECCCHHHHHHHH
LLQVDIETLRALNPQYKRDIVPGNTRPSVLKLPAAETYAFIDKEDTVYTHRIEELLANCI
HHHHHHHHHHHCCCHHHHCCCCCCCCCCEEECCCCCCEEEECCCCCHHHHHHHHHHHHHH
SANNSNGSLPSGATREKITHVVLSGENLYTIANRYGVTAKDIRKWNGLGSNRVAKGKRLR
CCCCCCCCCCCCCHHHHHEEEEECCCCEEEEHHHCCCCHHHHHHHCCCCCCCCCCCCEEE
LYVDNGGVAFASATKTPVKSTTVTAKSTATTTKKSASAATKQIASANKDFVSYKVKSGDS
EEEECCCEEEEECCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCC
LYSISKKYPGVTASSLQKVNGLSSPDIRPGQVLKIPVG
EEEHHHCCCCCCHHHHHHHCCCCCCCCCCCCEEEECCC
>Mature Secondary Structure
MKRYLLALICLNCLVSLVYAQEETRIEEKDTLSSLESEVGLIPESLDANVDSLLRTWHVQ
CHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
YFSKREDFCHDDDENVYFPDSVYIDRLSRLPSVIALPYNNIVRDCIDLYAERKRNLVRYM
HHHHCCCCCCCCCCCEECCCHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHH
LGMADFYFPIIEQVLDEHGLPIELKYLAVVESALNPVALSRVGACGLWQFMLPTGKSYGL
HHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHCCHHHHHHHCCHHHHHHHCCCCCCCCE
EINSLVDERRDPLKATEAACKYFKDMYAIYGDWNLVLASYNCGPGNVNKAIRRSGGKTDF
EHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHCCCCCCH
WDIFPYLPKETRSYVPLFIAANYVMNYYCEHNICPMQTSLPLATDTVMVNNALHLQQVSD
HHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEECEEEECCCHHHHHHHH
LLQVDIETLRALNPQYKRDIVPGNTRPSVLKLPAAETYAFIDKEDTVYTHRIEELLANCI
HHHHHHHHHHHCCCHHHHCCCCCCCCCCEEECCCCCCEEEECCCCCHHHHHHHHHHHHHH
SANNSNGSLPSGATREKITHVVLSGENLYTIANRYGVTAKDIRKWNGLGSNRVAKGKRLR
CCCCCCCCCCCCCHHHHHEEEEECCCCEEEEHHHCCCCHHHHHHHCCCCCCCCCCCCEEE
LYVDNGGVAFASATKTPVKSTTVTAKSTATTTKKSASAATKQIASANKDFVSYKVKSGDS
EEEECCCEEEEECCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCC
LYSISKKYPGVTASSLQKVNGLSSPDIRPGQVLKIPVG
EEEHHHCCCCCCHHHHHHHCCCCCCCCCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]