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The map label for this gene is mraW

Identifier: 150009093

GI number: 150009093

Start: 2940543

End: 2941460

Strand: Reverse

Name: mraW

Synonym: BDI_2494

Alternate gene names: 150009093

Gene position: 2941460-2940543 (Counterclockwise)

Preceding gene: 150009096

Following gene: 150009092

Centisome position: 61.14

GC content: 50.98

Gene sequence:

>918_bases
ATGGAAGAAAAAGATAGTTGCTATCATGTACCGGTAATGCTGCGGGAAAGTCTGGAGGGATTGGATATCCGTCCCGACGG
GATTTACGTGGATGTTACGTTTGGCGGTGGAGGACATTCCCGCGAGATATTGAAACGGTTGGGTAGCGAGGGCGAACTTT
ACGGCTTTGACCAAGATGCGGACGCCGAGCATAATGTCCCGGCAGACCCACGATTCACTTTCGTGCGCAGTAATTTCCGT
TATTTGTATAATTTTATGCGTTACTACGGAGAAAGTGGGGAGGTGGACGGCCTGTTGGCGGATTTAGGTGTCTCTTCCCA
TCATTTCGACGATAAGGATCGCGGTTTCTCTTTCCGTTTCGACGGGACGTTGGATATGCGGATGAATACGCGTGCGGGGC
AGACCGCCGCCGATATCGTGAATAATTATACGGCGGAGGCGTTGGCCGATGTGTTTTATCTATATGGCGAGCTGAAGGTG
TCTCGGAAATTAGCCTCTGTGCTGGTGAAAGCTCGTGAGACAAAGAAGATAGAGACGATCGGAGACTTCTTGGAGGTGAT
CAAGCCGTTTACGGGAAAGGACAAAGAGAAGAAATTCTTGGCGCAAGTCTTTCAGGCGCTTCGTATCGAGGTGAACGATG
AGATGAGGGCGCTGAAAGAGATGCTGCGGAGTACCTTGCGGGTGTTGAGACCGGGAGGACGATTGGTGGTTATCACGTAT
CACTCCTTGGAAGACCGTCTGGTAAAGAACTTTTTGAAGACCGGAAACTTCGAGGGGAAATGCGAGCAGGATTTCTTCGG
GAACGTACGATCGCCTTTCCGCTTGGTCAATAATAAGGTAATCGTCCCGACCGACGAGGAGGTGGAGCGGAATCCCCGTT
CCCGGAGTGCCAAGTTGAGAATCGCTGAGAAGGTATAA

Upstream 100 bases:

>100_bases
TTTGGAAATGTGTACTTTTACCCACCGATAAGGCATGTGCGTGCGTATCAAAGGAGTACGGCGCTTGATTTTAAGAGGAA
GTTTAACGTAAGAACGACGA

Downstream 100 bases:

>100_bases
GGAGAAATAGATATGGAAGAAAACGGACAACATAAGAAAACGAGGAAGAAGGAGAAGCGTCTTTCGTTCTGGTATATACT
CGGTGGCGGCGTGCTAAAAG

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MEEKDSCYHVPVMLRESLEGLDIRPDGIYVDVTFGGGGHSREILKRLGSEGELYGFDQDADAEHNVPADPRFTFVRSNFR
YLYNFMRYYGESGEVDGLLADLGVSSHHFDDKDRGFSFRFDGTLDMRMNTRAGQTAADIVNNYTAEALADVFYLYGELKV
SRKLASVLVKARETKKIETIGDFLEVIKPFTGKDKEKKFLAQVFQALRIEVNDEMRALKEMLRSTLRVLRPGGRLVVITY
HSLEDRLVKNFLKTGNFEGKCEQDFFGNVRSPFRLVNNKVIVPTDEEVERNPRSRSAKLRIAEKV

Sequences:

>Translated_305_residues
MEEKDSCYHVPVMLRESLEGLDIRPDGIYVDVTFGGGGHSREILKRLGSEGELYGFDQDADAEHNVPADPRFTFVRSNFR
YLYNFMRYYGESGEVDGLLADLGVSSHHFDDKDRGFSFRFDGTLDMRMNTRAGQTAADIVNNYTAEALADVFYLYGELKV
SRKLASVLVKARETKKIETIGDFLEVIKPFTGKDKEKKFLAQVFQALRIEVNDEMRALKEMLRSTLRVLRPGGRLVVITY
HSLEDRLVKNFLKTGNFEGKCEQDFFGNVRSPFRLVNNKVIVPTDEEVERNPRSRSAKLRIAEKV
>Mature_305_residues
MEEKDSCYHVPVMLRESLEGLDIRPDGIYVDVTFGGGGHSREILKRLGSEGELYGFDQDADAEHNVPADPRFTFVRSNFR
YLYNFMRYYGESGEVDGLLADLGVSSHHFDDKDRGFSFRFDGTLDMRMNTRAGQTAADIVNNYTAEALADVFYLYGELKV
SRKLASVLVKARETKKIETIGDFLEVIKPFTGKDKEKKFLAQVFQALRIEVNDEMRALKEMLRSTLRVLRPGGRLVVITY
HSLEDRLVKNFLKTGNFEGKCEQDFFGNVRSPFRLVNNKVIVPTDEEVERNPRSRSAKLRIAEKV

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=339, Percent_Identity=37.1681415929204, Blast_Score=183, Evalue=2e-46,
Organism=Homo sapiens, GI165377202, Length=194, Percent_Identity=36.5979381443299, Blast_Score=110, Evalue=2e-24,
Organism=Escherichia coli, GI1786270, Length=305, Percent_Identity=40.655737704918, Blast_Score=221, Evalue=7e-59,
Organism=Drosophila melanogaster, GI62472493, Length=334, Percent_Identity=35.9281437125749, Blast_Score=167, Evalue=1e-41,
Organism=Drosophila melanogaster, GI28571637, Length=334, Percent_Identity=35.9281437125749, Blast_Score=167, Evalue=1e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_PARD8 (A6LEV0)

Other databases:

- EMBL:   CP000140
- RefSeq:   YP_001303836.1
- ProteinModelPortal:   A6LEV0
- SMR:   A6LEV0
- STRING:   A6LEV0
- GeneID:   5307643
- GenomeReviews:   CP000140_GR
- KEGG:   pdi:BDI_2494
- eggNOG:   COG0275
- HOGENOM:   HBG302779
- OMA:   PQLDDPE
- ProtClustDB:   PRK00050
- BioCyc:   PDIS435591:BDI_2494-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 34876; Mature: 34876

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: NA

Important sites: BINDING 57-57 BINDING 85-85 BINDING 101-101 BINDING 108-108

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEKDSCYHVPVMLRESLEGLDIRPDGIYVDVTFGGGGHSREILKRLGSEGELYGFDQDA
CCCCCCEEECHHHHHHCCCCCCCCCCCEEEEEEECCCCCHHHHHHHCCCCCCEECCCCCC
DAEHNVPADPRFTFVRSNFRYLYNFMRYYGESGEVDGLLADLGVSSHHFDDKDRGFSFRF
CCCCCCCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCCCEEEEE
DGTLDMRMNTRAGQTAADIVNNYTAEALADVFYLYGELKVSRKLASVLVKARETKKIETI
CCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GDFLEVIKPFTGKDKEKKFLAQVFQALRIEVNDEMRALKEMLRSTLRVLRPGGRLVVITY
HHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEE
HSLEDRLVKNFLKTGNFEGKCEQDFFGNVRSPFRLVNNKVIVPTDEEVERNPRSRSAKLR
CCHHHHHHHHHHHCCCCCCCCCHHHHCCCCCHHHHHCCEEEECCCHHHHCCCCCCCCEEE
IAEKV
EECCC
>Mature Secondary Structure
MEEKDSCYHVPVMLRESLEGLDIRPDGIYVDVTFGGGGHSREILKRLGSEGELYGFDQDA
CCCCCCEEECHHHHHHCCCCCCCCCCCEEEEEEECCCCCHHHHHHHCCCCCCEECCCCCC
DAEHNVPADPRFTFVRSNFRYLYNFMRYYGESGEVDGLLADLGVSSHHFDDKDRGFSFRF
CCCCCCCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCCCEEEEE
DGTLDMRMNTRAGQTAADIVNNYTAEALADVFYLYGELKVSRKLASVLVKARETKKIETI
CCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GDFLEVIKPFTGKDKEKKFLAQVFQALRIEVNDEMRALKEMLRSTLRVLRPGGRLVVITY
HHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEE
HSLEDRLVKNFLKTGNFEGKCEQDFFGNVRSPFRLVNNKVIVPTDEEVERNPRSRSAKLR
CCHHHHHHHHHHHCCCCCCCCCHHHHCCCCCHHHHHCCEEEECCCHHHHCCCCCCCCEEE
IAEKV
EECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA