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The map label for this gene is eno [H]

Identifier: 150008955

GI number: 150008955

Start: 2773484

End: 2774767

Strand: Reverse

Name: eno [H]

Synonym: BDI_2351

Alternate gene names: 150008955

Gene position: 2774767-2773484 (Counterclockwise)

Preceding gene: 150008957

Following gene: 150008952

Centisome position: 57.67

GC content: 48.6

Gene sequence:

>1284_bases
ATGGAAATTGTAAAAGTAGTAGGTAGAGAGATCCTTGATTCACGTGGTAACCCGACAGTAGAAGTAGACGTACATTTGGC
TTCCGGCGCATTCGGTCGTGCGGCTGTACCTTCTGGCGCCTCTACCGGTGAGAACGAGGCTATCGAGTTACGCGATGGCG
ATAAGAATCGCTACGGTGGCAAAGGCGTTCTCCGTGCCGTGGATAATGTAAATAAGGTAATCGCTCCCGCCATTTTAGGA
ATGAGCGCCTTGAATCAAAGAGAGATAGACCATAAGTTGCTGGATTTGGATGGTACAAAGACAAAATCAAATCTAGGAGC
TAACGCTATGTTAGGTGTATCGCTTGCCGTAGCGAAGGCAGCGGCTAATTATCTGGATCTGCCTTTATATCGTTATATCG
GTGGAACAAATACCTACGTGTTGCCGGTTCCTATGATGAACATTATCAATGGAGGTTCTCACTCCGACGCTCCGATCGCT
TTTCAGGAATTCATGATTCGTCCGGTAGGTGCGAAGAGTTTCCGTGAGGGGCTTCGTATGGGCGCAGAGGTTTTCCATGC
GTTGAAGAAAGTATTGCACGATCGTGGCTTGAGCACGGCTGTAGGTGATGAGGGCGGTTTCGCTCCGGCATTGGATGGTA
CGGAAGATGCCTTGAATTCTATTATGGCCGCTATCAAAGCCGCTGGATATGAGCCGGGCAAGGATGTGACGATAGCCATG
GATTGCGCTGCTTCCGAGTTTTATCACGATGGTGTGTATGATTATACGAAATTCGAGGGTGAGAAAGGCGCTAAGCGTAA
CGCCCAGCAACAAGTCGCTTATCTGAGTGAGTTGGTGAACAAATACCCGATTGATTCTATCGAGGATGGTATGGACGAGA
ATGACTGGGCCGGTTGGCAAATGCTAACCTCCACGCTAGGCGGAAAATGCCAGTTGGTAGGTGATGACCTATTCGTTACA
AACGTAGAGTTCTTGAAGAAAGGTATCGAGAAGGGTTGCGCAAACTCCATCTTGATCAAGGTAAACCAGATCGGTACATT
GAGCGAGACATTAGACGCTATCGAGATGGCCCACCGTAATGGCTATACATCCGTGACTTCCCACCGTTCCGGTGAGACAG
AGGACGCTACGATCGCCGATATCGCCGTAGCTACCAACAGCGGACAGATCAAGACCGGCTCATTGAGCCGTTCCGATCGT
ATGGCGAAATACAACCAGTTGCTTCGTATCGAAGAGGAATTGGGTTCCTTAGCTGTTTATGGTTATAAGACTTATAAGAA
GTAA

Upstream 100 bases:

>100_bases
TCGATAGGCTTCCGCAAACATTCCTTGCTTGGCTATTGTTATATTTGTGAAAAAGAAAAGTAAGACATCATTGAACAACT
AATAAAACAAATATAGTAAT

Downstream 100 bases:

>100_bases
TTTTCATTATTTGCTTGAGAAATGGGGGAGTTCACCTATCAAGGTGGACTGCCTTTTTCTTTTTAGTGGAAAAAACGAAG
CAAGCCTACTAATCAATAGC

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MEIVKVVGREILDSRGNPTVEVDVHLASGAFGRAAVPSGASTGENEAIELRDGDKNRYGGKGVLRAVDNVNKVIAPAILG
MSALNQREIDHKLLDLDGTKTKSNLGANAMLGVSLAVAKAAANYLDLPLYRYIGGTNTYVLPVPMMNIINGGSHSDAPIA
FQEFMIRPVGAKSFREGLRMGAEVFHALKKVLHDRGLSTAVGDEGGFAPALDGTEDALNSIMAAIKAAGYEPGKDVTIAM
DCAASEFYHDGVYDYTKFEGEKGAKRNAQQQVAYLSELVNKYPIDSIEDGMDENDWAGWQMLTSTLGGKCQLVGDDLFVT
NVEFLKKGIEKGCANSILIKVNQIGTLSETLDAIEMAHRNGYTSVTSHRSGETEDATIADIAVATNSGQIKTGSLSRSDR
MAKYNQLLRIEEELGSLAVYGYKTYKK

Sequences:

>Translated_427_residues
MEIVKVVGREILDSRGNPTVEVDVHLASGAFGRAAVPSGASTGENEAIELRDGDKNRYGGKGVLRAVDNVNKVIAPAILG
MSALNQREIDHKLLDLDGTKTKSNLGANAMLGVSLAVAKAAANYLDLPLYRYIGGTNTYVLPVPMMNIINGGSHSDAPIA
FQEFMIRPVGAKSFREGLRMGAEVFHALKKVLHDRGLSTAVGDEGGFAPALDGTEDALNSIMAAIKAAGYEPGKDVTIAM
DCAASEFYHDGVYDYTKFEGEKGAKRNAQQQVAYLSELVNKYPIDSIEDGMDENDWAGWQMLTSTLGGKCQLVGDDLFVT
NVEFLKKGIEKGCANSILIKVNQIGTLSETLDAIEMAHRNGYTSVTSHRSGETEDATIADIAVATNSGQIKTGSLSRSDR
MAKYNQLLRIEEELGSLAVYGYKTYKK
>Mature_427_residues
MEIVKVVGREILDSRGNPTVEVDVHLASGAFGRAAVPSGASTGENEAIELRDGDKNRYGGKGVLRAVDNVNKVIAPAILG
MSALNQREIDHKLLDLDGTKTKSNLGANAMLGVSLAVAKAAANYLDLPLYRYIGGTNTYVLPVPMMNIINGGSHSDAPIA
FQEFMIRPVGAKSFREGLRMGAEVFHALKKVLHDRGLSTAVGDEGGFAPALDGTEDALNSIMAAIKAAGYEPGKDVTIAM
DCAASEFYHDGVYDYTKFEGEKGAKRNAQQQVAYLSELVNKYPIDSIEDGMDENDWAGWQMLTSTLGGKCQLVGDDLFVT
NVEFLKKGIEKGCANSILIKVNQIGTLSETLDAIEMAHRNGYTSVTSHRSGETEDATIADIAVATNSGQIKTGSLSRSDR
MAKYNQLLRIEEELGSLAVYGYKTYKK

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI5803011, Length=435, Percent_Identity=52.6436781609195, Blast_Score=434, Evalue=1e-121,
Organism=Homo sapiens, GI301897477, Length=435, Percent_Identity=51.9540229885057, Blast_Score=421, Evalue=1e-118,
Organism=Homo sapiens, GI301897469, Length=435, Percent_Identity=51.9540229885057, Blast_Score=421, Evalue=1e-118,
Organism=Homo sapiens, GI4503571, Length=434, Percent_Identity=50.4608294930876, Blast_Score=413, Evalue=1e-115,
Organism=Homo sapiens, GI301897479, Length=432, Percent_Identity=47.4537037037037, Blast_Score=362, Evalue=1e-100,
Organism=Homo sapiens, GI169201331, Length=347, Percent_Identity=27.9538904899135, Blast_Score=115, Evalue=7e-26,
Organism=Homo sapiens, GI169201757, Length=347, Percent_Identity=27.9538904899135, Blast_Score=115, Evalue=7e-26,
Organism=Homo sapiens, GI239744207, Length=347, Percent_Identity=27.9538904899135, Blast_Score=115, Evalue=7e-26,
Organism=Escherichia coli, GI1789141, Length=423, Percent_Identity=62.1749408983452, Blast_Score=503, Evalue=1e-144,
Organism=Caenorhabditis elegans, GI71995829, Length=435, Percent_Identity=52.4137931034483, Blast_Score=415, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI17536383, Length=435, Percent_Identity=52.4137931034483, Blast_Score=415, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI32563855, Length=251, Percent_Identity=41.4342629482072, Blast_Score=174, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6321693, Length=436, Percent_Identity=50, Blast_Score=394, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6323985, Length=431, Percent_Identity=48.9559164733179, Blast_Score=394, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6324974, Length=431, Percent_Identity=48.9559164733179, Blast_Score=393, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6324969, Length=431, Percent_Identity=48.9559164733179, Blast_Score=393, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6321968, Length=436, Percent_Identity=49.5412844036697, Blast_Score=362, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580918, Length=437, Percent_Identity=50.3432494279176, Blast_Score=390, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580916, Length=437, Percent_Identity=50.3432494279176, Blast_Score=390, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580920, Length=437, Percent_Identity=50.3432494279176, Blast_Score=390, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580914, Length=437, Percent_Identity=50.3432494279176, Blast_Score=390, Evalue=1e-108,
Organism=Drosophila melanogaster, GI281360527, Length=437, Percent_Identity=50.3432494279176, Blast_Score=389, Evalue=1e-108,
Organism=Drosophila melanogaster, GI17137654, Length=437, Percent_Identity=50.3432494279176, Blast_Score=389, Evalue=1e-108,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 45870; Mature: 45870

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS00164 ENOLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIVKVVGREILDSRGNPTVEVDVHLASGAFGRAAVPSGASTGENEAIELRDGDKNRYGG
CHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCC
KGVLRAVDNVNKVIAPAILGMSALNQREIDHKLLDLDGTKTKSNLGANAMLGVSLAVAKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHH
AANYLDLPLYRYIGGTNTYVLPVPMMNIINGGSHSDAPIAFQEFMIRPVGAKSFREGLRM
HHHHHCCCHHHHHCCCCCEEEECCHHHHHCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHH
GAEVFHALKKVLHDRGLSTAVGDEGGFAPALDGTEDALNSIMAAIKAAGYEPGKDVTIAM
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEE
DCAASEFYHDGVYDYTKFEGEKGAKRNAQQQVAYLSELVNKYPIDSIEDGMDENDWAGWQ
ECHHHHHHHCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHH
MLTSTLGGKCQLVGDDLFVTNVEFLKKGIEKGCANSILIKVNQIGTLSETLDAIEMAHRN
HHHHHCCCEEEEEECCEEEHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHC
GYTSVTSHRSGETEDATIADIAVATNSGQIKTGSLSRSDRMAKYNQLLRIEEELGSLAVY
CCCCCCCCCCCCCCCCEEEEEEEEECCCCEEECCCCCHHHHHHHHHHHHHHHHHCCEEEE
GYKTYKK
EEHHCCC
>Mature Secondary Structure
MEIVKVVGREILDSRGNPTVEVDVHLASGAFGRAAVPSGASTGENEAIELRDGDKNRYGG
CHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCC
KGVLRAVDNVNKVIAPAILGMSALNQREIDHKLLDLDGTKTKSNLGANAMLGVSLAVAKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHH
AANYLDLPLYRYIGGTNTYVLPVPMMNIINGGSHSDAPIAFQEFMIRPVGAKSFREGLRM
HHHHHCCCHHHHHCCCCCEEEECCHHHHHCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHH
GAEVFHALKKVLHDRGLSTAVGDEGGFAPALDGTEDALNSIMAAIKAAGYEPGKDVTIAM
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEE
DCAASEFYHDGVYDYTKFEGEKGAKRNAQQQVAYLSELVNKYPIDSIEDGMDENDWAGWQ
ECHHHHHHHCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHH
MLTSTLGGKCQLVGDDLFVTNVEFLKKGIEKGCANSILIKVNQIGTLSETLDAIEMAHRN
HHHHHCCCEEEEEECCEEEHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHC
GYTSVTSHRSGETEDATIADIAVATNSGQIKTGSLSRSDRMAKYNQLLRIEEELGSLAVY
CCCCCCCCCCCCCCCCEEEEEEEEECCCCEEECCCCCHHHHHHHHHHHHHHHHHCCEEEE
GYKTYKK
EEHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA