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The map label for this gene is priA [H]

Identifier: 150008903

GI number: 150008903

Start: 2691976

End: 2694438

Strand: Reverse

Name: priA [H]

Synonym: BDI_2298

Alternate gene names: 150008903

Gene position: 2694438-2691976 (Counterclockwise)

Preceding gene: 150008904

Following gene: 150008902

Centisome position: 56.0

GC content: 52.78

Gene sequence:

>2463_bases
ATGAAATATGCGGACGTCATACTTCCCCTTCCTTTGGAGAATAGCTATACCTATCGGATACCCGAAGATATGGAAAGAGC
CGTGACTCCCGGTTGCCGGGTGATCGTGCATTTCGGGAAGAAACGATATTATACGGCCATCGTCATGGAGGTACACGACC
GGGAACCGGTATCCGATTTTGAGACGAAGGAGATCTACGCTTTGCTGGATGCCACTCCGGTGCTCCGCCGTCCGCAACTC
CGTTTCTGGAAATGGATATCTTCTTATTATATATGTAAGCTGGGCGACGTGTATAAGGCGGCCTTGCCTTCCGGCTTAAA
GTTGGAAAGCGAGACGGCGGTTACTTATAATGAGGATTTCGAGGCTACCGCCCCCTTGCGCCCGAACGAGCAAGCGGTAC
TGGATGCCTTTAGCGGGGTTTTGAAATTGACGATCTCGGAGTTGGAGAAGAAGACCGGTCTCCGAAATGTTGTCCCGATC
GTCTCTTCCTTGATGGTGAAAGGGGCGGTCGAGGTGAGCGAGGAGCTAAAGCGAGGATTCGTTCCCAAGACACAGGCGTT
TATCCGTTTGGCCGAAGCTTACTGGGATGAGACGAAATTGCAGGCGGTGTTCGAAGAATTGAAAAGGGCGAAGAAGCAAG
AGCTTCTGTTGGTCAGCTTCTTGGATTTAAGCCATACCTTGAATCCCGCTTTATCGAAGGAGCTTTCCAAGAAAGAGCTA
TTGGAACGCAGTGGTTATACCTCTGCGGTATTGGAGGGTCTGCTGAAACGGGGTATCTTGGAGAGTTACGAGAAAGAGGT
GGGTCGTTTACAAGTATCCGTCTGCCGCTTGCAAGAGCCGAATCCGCTCAGTCCGGCGCAAGAGAAAGCCTACGGAGAGA
TCCATGAGGCTTTCAAGACGAAGGAGGTTTGCTTGCTTCATGGCGTGACTTCCAGTGGTAAGACCGAGATATACGTCCGT
TTGATCCATGAGGTCTTGCGGCTGGGGCGGCAGGTTTTATATATGTTGCCGGAGATCGCTATCACGACCCAGATCACGGA
ACGGCTGGCGAAATTATTCGGGGATAAGCTATTGGTCTACCACTCTAAGTTCTCGGACAACGAGCGGGTGGAGGTCTGGA
ACAAGCTGCTGCATAGCGACGAGCCTATGCTGGTATTGGGGGTCCGTTCTTCCCTCTTCCTTCCGTTTAAGGACTTGGGC
TTGATTATCGTGGACGAGGAGCATGAGAATACGTATAAGCAGCAAGACCCGGCACCCCGTTACCATGCCCGTAACGCTGC
CATCGTCTTGGCGGGTATGCACGGCGGTAAGACCTTATTGGGTTCCGCCACCCCTTCCATCGACTCTTATTTCAACGCTA
CGGCGGGGAAATACGGTCTGGTCGAGCTATCCACCCGCTACGGTGATTGCTTGATGCCGGAGATTATTACGGTGGACGTG
AAGGAGCTAAAGCGCAAGAAGATTATGAAAGATACCCTTTTCTCCCCCCTGTTGGTGGAGAAGGTGCGGGAGGCTTTATC
CCGGGGTGAGCAAGCGATCTTGTTCCAGAACCGCCGGGGATTCGCCCCGATGATCGAGTGTCGGGGGTGCGGGTGGGTGC
CTCATTGCGTGAATTGCGACGTTAGCCTTACCTACCACAAGGCGCATAATCAGCTGGTTTGCCATTATTGCGGTTATACC
TACCGCTTGCCGCAGGTATGTCCAGAGTGTAAGGGGACGGAGTTCAAGATGCAAGGCTTCGGTACGGAGAAGGTAGAGGA
GGAGATCGCTGGGCAATTCCCAGCGGCGAAGGTGGAGCGGCTGGATTTCGATACGGCCCGTACCCGCACGGCTTACGAGC
GGATCATCTCGGATTTCGAGAAGGGGAAGACACAAATCTTGATTGGTACCCAGATGTTGTCGAAGGGATTGGATTTCGGG
AATGTCAGCGTGGTCGGAATCTTGAGCGCCGATAGCCTGATGAATTTCCCAGACTTCCGGGCGCACGAGCGGGCTTATCA
ATTGATGGTACAGGTTAGCGGACGTGCCGGACGACGGGATAAGCGGGGTACGGTGATCTTGCAGACTACTCAGCCGGAGC
ATCCTTTGATCCGTATGGTGCAGCATTTTGCTTATAAGGAAATGGTGAGCTTGCAATTGAGCGAGCGTAGTATGTTCCGT
TACCCGCCTTACTACCGTTTGATCGTCTTGATCCTTCGGAGTCGGAATGAGGAGGCTTTGCAGGAAATGTCTGCTTTATA
CGCCGAGAAACTTCGTGCTCGTTTGGGTGAGCGGGTGTTGGGGCCGGTTACGCCGCCTATCACCCGGGTACAGACGCTTC
ATATCAAGAAGATCGTCTTGAAGATCGAGATATCCGCCGGGATCGCTCCGCTTCGTGAGATATTGGAGGGTATTCATACT
GAGATGCAAGGATATGTACCGTTTAAGCAGTTGCTGGTTCATTATGATGTGGACCCGGCTTGA

Upstream 100 bases:

>100_bases
GATCTTGTTCTAAATAGGTGAAAATAAAATGAGAGAGGCTGGGCTTTAGAAGTCCGGCCTTTTTTGTTATTTTTGGGCTC
GTAATGAAACACCTGTTTGA

Downstream 100 bases:

>100_bases
GAATTGAAAATGGAGAATTGAAAATTGAAAATTAATGGAAAGGAGCTTAGTGATGAAGGAGGAGAAGGTACGGTTGTTGT
TTGTGTGCTTGGGGAATATA

Product: primosomal protein N'

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 820; Mature: 820

Protein sequence:

>820_residues
MKYADVILPLPLENSYTYRIPEDMERAVTPGCRVIVHFGKKRYYTAIVMEVHDREPVSDFETKEIYALLDATPVLRRPQL
RFWKWISSYYICKLGDVYKAALPSGLKLESETAVTYNEDFEATAPLRPNEQAVLDAFSGVLKLTISELEKKTGLRNVVPI
VSSLMVKGAVEVSEELKRGFVPKTQAFIRLAEAYWDETKLQAVFEELKRAKKQELLLVSFLDLSHTLNPALSKELSKKEL
LERSGYTSAVLEGLLKRGILESYEKEVGRLQVSVCRLQEPNPLSPAQEKAYGEIHEAFKTKEVCLLHGVTSSGKTEIYVR
LIHEVLRLGRQVLYMLPEIAITTQITERLAKLFGDKLLVYHSKFSDNERVEVWNKLLHSDEPMLVLGVRSSLFLPFKDLG
LIIVDEEHENTYKQQDPAPRYHARNAAIVLAGMHGGKTLLGSATPSIDSYFNATAGKYGLVELSTRYGDCLMPEIITVDV
KELKRKKIMKDTLFSPLLVEKVREALSRGEQAILFQNRRGFAPMIECRGCGWVPHCVNCDVSLTYHKAHNQLVCHYCGYT
YRLPQVCPECKGTEFKMQGFGTEKVEEEIAGQFPAAKVERLDFDTARTRTAYERIISDFEKGKTQILIGTQMLSKGLDFG
NVSVVGILSADSLMNFPDFRAHERAYQLMVQVSGRAGRRDKRGTVILQTTQPEHPLIRMVQHFAYKEMVSLQLSERSMFR
YPPYYRLIVLILRSRNEEALQEMSALYAEKLRARLGERVLGPVTPPITRVQTLHIKKIVLKIEISAGIAPLREILEGIHT
EMQGYVPFKQLLVHYDVDPA

Sequences:

>Translated_820_residues
MKYADVILPLPLENSYTYRIPEDMERAVTPGCRVIVHFGKKRYYTAIVMEVHDREPVSDFETKEIYALLDATPVLRRPQL
RFWKWISSYYICKLGDVYKAALPSGLKLESETAVTYNEDFEATAPLRPNEQAVLDAFSGVLKLTISELEKKTGLRNVVPI
VSSLMVKGAVEVSEELKRGFVPKTQAFIRLAEAYWDETKLQAVFEELKRAKKQELLLVSFLDLSHTLNPALSKELSKKEL
LERSGYTSAVLEGLLKRGILESYEKEVGRLQVSVCRLQEPNPLSPAQEKAYGEIHEAFKTKEVCLLHGVTSSGKTEIYVR
LIHEVLRLGRQVLYMLPEIAITTQITERLAKLFGDKLLVYHSKFSDNERVEVWNKLLHSDEPMLVLGVRSSLFLPFKDLG
LIIVDEEHENTYKQQDPAPRYHARNAAIVLAGMHGGKTLLGSATPSIDSYFNATAGKYGLVELSTRYGDCLMPEIITVDV
KELKRKKIMKDTLFSPLLVEKVREALSRGEQAILFQNRRGFAPMIECRGCGWVPHCVNCDVSLTYHKAHNQLVCHYCGYT
YRLPQVCPECKGTEFKMQGFGTEKVEEEIAGQFPAAKVERLDFDTARTRTAYERIISDFEKGKTQILIGTQMLSKGLDFG
NVSVVGILSADSLMNFPDFRAHERAYQLMVQVSGRAGRRDKRGTVILQTTQPEHPLIRMVQHFAYKEMVSLQLSERSMFR
YPPYYRLIVLILRSRNEEALQEMSALYAEKLRARLGERVLGPVTPPITRVQTLHIKKIVLKIEISAGIAPLREILEGIHT
EMQGYVPFKQLLVHYDVDPA
>Mature_820_residues
MKYADVILPLPLENSYTYRIPEDMERAVTPGCRVIVHFGKKRYYTAIVMEVHDREPVSDFETKEIYALLDATPVLRRPQL
RFWKWISSYYICKLGDVYKAALPSGLKLESETAVTYNEDFEATAPLRPNEQAVLDAFSGVLKLTISELEKKTGLRNVVPI
VSSLMVKGAVEVSEELKRGFVPKTQAFIRLAEAYWDETKLQAVFEELKRAKKQELLLVSFLDLSHTLNPALSKELSKKEL
LERSGYTSAVLEGLLKRGILESYEKEVGRLQVSVCRLQEPNPLSPAQEKAYGEIHEAFKTKEVCLLHGVTSSGKTEIYVR
LIHEVLRLGRQVLYMLPEIAITTQITERLAKLFGDKLLVYHSKFSDNERVEVWNKLLHSDEPMLVLGVRSSLFLPFKDLG
LIIVDEEHENTYKQQDPAPRYHARNAAIVLAGMHGGKTLLGSATPSIDSYFNATAGKYGLVELSTRYGDCLMPEIITVDV
KELKRKKIMKDTLFSPLLVEKVREALSRGEQAILFQNRRGFAPMIECRGCGWVPHCVNCDVSLTYHKAHNQLVCHYCGYT
YRLPQVCPECKGTEFKMQGFGTEKVEEEIAGQFPAAKVERLDFDTARTRTAYERIISDFEKGKTQILIGTQMLSKGLDFG
NVSVVGILSADSLMNFPDFRAHERAYQLMVQVSGRAGRRDKRGTVILQTTQPEHPLIRMVQHFAYKEMVSLQLSERSMFR
YPPYYRLIVLILRSRNEEALQEMSALYAEKLRARLGERVLGPVTPPITRVQTLHIKKIVLKIEISAGIAPLREILEGIHT
EMQGYVPFKQLLVHYDVDPA

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=563, Percent_Identity=37.4777975133215, Blast_Score=363, Evalue=1e-101,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 93244; Mature: 93244

Theoretical pI: Translated: 8.42; Mature: 8.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYADVILPLPLENSYTYRIPEDMERAVTPGCRVIVHFGKKRYYTAIVMEVHDREPVSDF
CCCCCEEEECCCCCCEEECCCHHHHHHCCCCCEEEEECCCCEEEEEEEEEECCCCCCCCC
ETKEIYALLDATPVLRRPQLRFWKWISSYYICKLGDVYKAALPSGLKLESETAVTYNEDF
CHHHHHHHHCCCCHHCCCHHHHHHHHHHHHEEECCHHHHHHCCCCCEECCCCEEEECCCC
EATAPLRPNEQAVLDAFSGVLKLTISELEKKTGLRNVVPIVSSLMVKGAVEVSEELKRGF
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
VPKTQAFIRLAEAYWDETKLQAVFEELKRAKKQELLLVSFLDLSHTLNPALSKELSKKEL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
LERSGYTSAVLEGLLKRGILESYEKEVGRLQVSVCRLQEPNPLSPAQEKAYGEIHEAFKT
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEHEECCCCCCCCHHHHHHHHHHHHHHHC
KEVCLLHGVTSSGKTEIYVRLIHEVLRLGRQVLYMLPEIAITTQITERLAKLFGDKLLVY
CCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEE
HSKFSDNERVEVWNKLLHSDEPMLVLGVRSSLFLPFKDLGLIIVDEEHENTYKQQDPAPR
ECCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHCCEEEEECCCCCHHCCCCCCCC
YHARNAAIVLAGMHGGKTLLGSATPSIDSYFNATAGKYGLVELSTRYGDCLMPEIITVDV
CCCCCCEEEEEECCCCCEEECCCCCCHHHHHCCCCCCCEEEEEECCCCCCCCCHHEEECH
KELKRKKIMKDTLFSPLLVEKVREALSRGEQAILFQNRRGFAPMIECRGCGWVPHCVNCD
HHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEECCCCCCCHHCCCC
VSLTYHKAHNQLVCHYCGYTYRLPQVCPECKGTEFKMQGFGTEKVEEEIAGQFPAAKVER
EEEEEECCCCCEEEEECCCCEECHHHCCCCCCCEEEECCCCHHHHHHHHHCCCCCHHHHH
LDFDTARTRTAYERIISDFEKGKTQILIGTQMLSKGLDFGNVSVVGILSADSLMNFPDFR
CCCCHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHCCCCCCCEEEEEEEEHHHHCCCCCCH
AHERAYQLMVQVSGRAGRRDKRGTVILQTTQPEHPLIRMVQHFAYKEMVSLQLSERSMFR
HHHHHHHHHHEECCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
YPPYYRLIVLILRSRNEEALQEMSALYAEKLRARLGERVLGPVTPPITRVQTLHIKKIVL
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHEEEEEEE
KIEISAGIAPLREILEGIHTEMQGYVPFKQLLVHYDVDPA
EEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCC
>Mature Secondary Structure
MKYADVILPLPLENSYTYRIPEDMERAVTPGCRVIVHFGKKRYYTAIVMEVHDREPVSDF
CCCCCEEEECCCCCCEEECCCHHHHHHCCCCCEEEEECCCCEEEEEEEEEECCCCCCCCC
ETKEIYALLDATPVLRRPQLRFWKWISSYYICKLGDVYKAALPSGLKLESETAVTYNEDF
CHHHHHHHHCCCCHHCCCHHHHHHHHHHHHEEECCHHHHHHCCCCCEECCCCEEEECCCC
EATAPLRPNEQAVLDAFSGVLKLTISELEKKTGLRNVVPIVSSLMVKGAVEVSEELKRGF
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
VPKTQAFIRLAEAYWDETKLQAVFEELKRAKKQELLLVSFLDLSHTLNPALSKELSKKEL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
LERSGYTSAVLEGLLKRGILESYEKEVGRLQVSVCRLQEPNPLSPAQEKAYGEIHEAFKT
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEHEECCCCCCCCHHHHHHHHHHHHHHHC
KEVCLLHGVTSSGKTEIYVRLIHEVLRLGRQVLYMLPEIAITTQITERLAKLFGDKLLVY
CCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEE
HSKFSDNERVEVWNKLLHSDEPMLVLGVRSSLFLPFKDLGLIIVDEEHENTYKQQDPAPR
ECCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHCCEEEEECCCCCHHCCCCCCCC
YHARNAAIVLAGMHGGKTLLGSATPSIDSYFNATAGKYGLVELSTRYGDCLMPEIITVDV
CCCCCCEEEEEECCCCCEEECCCCCCHHHHHCCCCCCCEEEEEECCCCCCCCCHHEEECH
KELKRKKIMKDTLFSPLLVEKVREALSRGEQAILFQNRRGFAPMIECRGCGWVPHCVNCD
HHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEECCCCCCCHHCCCC
VSLTYHKAHNQLVCHYCGYTYRLPQVCPECKGTEFKMQGFGTEKVEEEIAGQFPAAKVER
EEEEEECCCCCEEEEECCCCEECHHHCCCCCCCEEEECCCCHHHHHHHHHCCCCCHHHHH
LDFDTARTRTAYERIISDFEKGKTQILIGTQMLSKGLDFGNVSVVGILSADSLMNFPDFR
CCCCHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHCCCCCCCEEEEEEEEHHHHCCCCCCH
AHERAYQLMVQVSGRAGRRDKRGTVILQTTQPEHPLIRMVQHFAYKEMVSLQLSERSMFR
HHHHHHHHHHEECCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
YPPYYRLIVLILRSRNEEALQEMSALYAEKLRARLGERVLGPVTPPITRVQTLHIKKIVL
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHEEEEEEE
KIEISAGIAPLREILEGIHTEMQGYVPFKQLLVHYDVDPA
EEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377; 9086272 [H]